Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0301
(687 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09020.1 68414.m01006 protein kinase, putative similar to pro... 44 7e-05
At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 ... 40 0.001
At4g16360.1 68417.m02478 5'-AMP-activated protein kinase beta-2 ... 40 0.002
At3g52180.1 68416.m05729 protein tyrosine phosphatase/kinase int... 39 0.004
At1g27070.1 68414.m03300 5'-AMP-activated protein kinase-related... 36 0.033
At5g39790.1 68418.m04820 5'-AMP-activated protein kinase beta-1 ... 32 0.41
At4g10720.1 68417.m01752 ankyrin repeat family protein contains ... 30 1.3
At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2 ... 30 1.7
At5g02170.1 68418.m00138 amino acid transporter family protein b... 29 2.9
At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low sim... 29 2.9
>At1g09020.1 68414.m01006 protein kinase, putative similar to
protein kinase AKINbetagamma-2 [Zea mays] GI:11139548,
AKINbetagamma-1 [Zea mays] GI:11139546; contains Pfam
profile PF00571: CBS domain
Length = 487
Score = 44.4 bits (100), Expect = 7e-05
Identities = 18/41 (43%), Positives = 23/41 (56%)
Frame = +1
Query: 514 FVTIIDLPEGEHQYQYFVDGEWRHDPTVKVIDNGMGAQNNL 636
F I +L G HQY++FVDGEWRHD + G N +
Sbjct: 59 FQVICNLTPGYHQYKFFVDGEWRHDEHQPFVSGNGGVVNTI 99
Score = 36.7 bits (81), Expect = 0.014
Identities = 15/28 (53%), Positives = 21/28 (75%), Gaps = 1/28 (3%)
Frame = +2
Query: 416 PTVFKWEGGGKQVFISGTFTDW-KTIPM 496
PT F W GG++VF+SG+FT W + +PM
Sbjct: 22 PTRFVWPYGGRRVFLSGSFTRWTEHVPM 49
>At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1
subunit-related contains similarity to Swiss-Prot:P80387
5'-AMP-activated protein kinase, beta-1 subunit (AMPK
beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa]
Length = 591
Score = 40.3 bits (90), Expect = 0.001
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +1
Query: 514 FVTIIDLPEGEHQYQYFVDGEWRHDPTVKVIDNGMGAQNNLVTV 645
F T + L +G++ Y+Y ++G+WRH T + G NN++ V
Sbjct: 493 FETEVRLTQGKYYYKYIINGDWRHSATSPTERDDRGNTNNIIVV 536
>At4g16360.1 68417.m02478 5'-AMP-activated protein kinase beta-2
subunit, putative similar to Swiss-Prot:Q9QZH4
5'-AMP-activated protein kinase, beta-2 subunit (AMPK
beta-2 chain) [Rattus norvegicus]
Length = 259
Score = 39.9 bits (89), Expect = 0.002
Identities = 15/46 (32%), Positives = 28/46 (60%)
Frame = +1
Query: 511 DFVTIIDLPEGEHQYQYFVDGEWRHDPTVKVIDNGMGAQNNLVTVK 648
DF + LP G ++Y++ VDG+WRH P + + + G N++ ++
Sbjct: 106 DFTIMKVLPSGVYEYRFIVDGQWRHAPELPLARDDAGNTFNILDLQ 151
Score = 32.7 bits (71), Expect = 0.23
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +2
Query: 413 LPTVFKWEGGGKQVFISGTFTDWKT 487
+PT+ W GGK++ + G++ +WKT
Sbjct: 72 IPTMITWCHGGKEIAVEGSWDNWKT 96
>At3g52180.1 68416.m05729 protein tyrosine phosphatase/kinase
interaction sequence protein (PTPKIS1) identical to
PTPKIS1 protein [Arabidopsis thaliana] GI:11595504;
contains Pfam profile PF00782: Dual specificity
phosphatase, catalytic domain
Length = 379
Score = 38.7 bits (86), Expect = 0.004
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +1
Query: 529 DLPEGEHQYQYFVDGEWRHDPTVKVI-DNGMGAQNNLVTV 645
+LPEG+ +Y+Y +DGEW H+ I N G NN V
Sbjct: 298 ELPEGQFEYKYIIDGEWTHNEAEPFIGPNKDGHTNNYAKV 337
>At1g27070.1 68414.m03300 5'-AMP-activated protein kinase-related
contains weak similarity to Swiss-Prot:Q9QZH4
5'-AMP-activated protein kinase, beta-2 subunit (AMPK
beta-2 chain) [Rattus norvegicus]
Length = 532
Score = 35.5 bits (78), Expect = 0.033
Identities = 14/44 (31%), Positives = 28/44 (63%)
Frame = +1
Query: 514 FVTIIDLPEGEHQYQYFVDGEWRHDPTVKVIDNGMGAQNNLVTV 645
F + L G+++ ++ VDG+W+ DP ++ +G G +NNL+ +
Sbjct: 489 FSLSLKLYPGKYEIKFIVDGQWKVDPLRPIVTSG-GYENNLLII 531
>At5g39790.1 68418.m04820 5'-AMP-activated protein kinase beta-1
subunit-related contains similarity to Swiss-Prot:P80387
5'-AMP-activated protein kinase, beta-1 subunit (AMPK
beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa]
Length = 273
Score = 31.9 bits (69), Expect = 0.41
Identities = 14/45 (31%), Positives = 25/45 (55%)
Frame = +1
Query: 514 FVTIIDLPEGEHQYQYFVDGEWRHDPTVKVIDNGMGAQNNLVTVK 648
F T + L G ++ ++ VDGEW+ P G+ +NN++ V+
Sbjct: 230 FSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGL-MENNVLVVE 273
>At4g10720.1 68417.m01752 ankyrin repeat family protein contains
Pfam domain, PF00023: Ankyrin repeat
Length = 445
Score = 30.3 bits (65), Expect = 1.3
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = -2
Query: 626 WAPIPLSITFTVGSCLHSPSTKYWY*CSPSGKSIIVTKSHVIL 498
W +PL +++ V + SP T WY + +G IIV +++++
Sbjct: 371 WIAVPLYVSYLVSMSVISPDT-VWYVSTNAGSVIIVVFAYMVV 412
>At5g21170.1 68418.m02530 5'-AMP-activated protein kinase beta-2
subunit, putative similar to Swiss-Prot:Q9QZH4
5'-AMP-activated protein kinase, beta-2 subunit (AMPK
beta-2 chain) [Rattus norvegicus]
Length = 283
Score = 29.9 bits (64), Expect = 1.7
Identities = 8/25 (32%), Positives = 16/25 (64%)
Frame = +2
Query: 413 LPTVFKWEGGGKQVFISGTFTDWKT 487
+PT+ W GG V + G++ +W++
Sbjct: 100 IPTIITWNQGGNDVAVEGSWDNWRS 124
Score = 28.3 bits (60), Expect = 5.0
Identities = 13/45 (28%), Positives = 24/45 (53%)
Frame = +1
Query: 511 DFVTIIDLPEGEHQYQYFVDGEWRHDPTVKVIDNGMGAQNNLVTV 645
D + LP G + Y+ VDGE ++ P + + + +G N++ V
Sbjct: 134 DHSILFVLPSGIYHYKVIVDGESKYIPDLPFVADEVGNVCNILDV 178
>At5g02170.1 68418.m00138 amino acid transporter family protein
belongs to INTERPRO:IPR002422 amino acid/polyamine
transporter, family II
Length = 526
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/51 (29%), Positives = 23/51 (45%), Gaps = 1/51 (1%)
Frame = -1
Query: 624 GPHSIVNYFHCRIMPPFSINKILVLMFTFW*IYYCNKITCDFTM-GIVFQS 475
G HS+ + + P +L++ F F ++Y C FTM G QS
Sbjct: 332 GSHSVFPNIYSSMKEPSKFPTVLLISFAFCTLFYIAVAVCGFTMFGDAIQS 382
>At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low
similarity to breast cancer susceptibility protein
[Gallus gallus] GI:19568157; contains Pfam profile
PF00634: BRCA2 repeat
Length = 1155
Score = 29.1 bits (62), Expect = 2.9
Identities = 19/73 (26%), Positives = 39/73 (53%), Gaps = 3/73 (4%)
Frame = +1
Query: 436 RRWKTSIYFWNIYRL---ENYSHGKITCDFVTIIDLPEGEHQYQYFVDGEWRHDPTVKVI 606
R+W T+ Y W +++L + Y K +F+TI ++ E E +Y+Y + H +K I
Sbjct: 575 RKWVTNHYRWIVWKLACYDIYYPAKCRGNFLTITNVLE-ELKYRYEREVNHGHCSAIKRI 633
Query: 607 DNGMGAQNNLVTV 645
+G ++++ +
Sbjct: 634 LSGDAPASSMMVL 646
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,591,441
Number of Sequences: 28952
Number of extensions: 306040
Number of successful extensions: 768
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 753
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 767
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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