Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0299
(478 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 134 7e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 87 1e-18
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 85 4e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 66 2e-12
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 28 0.64
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.5
SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 4.5
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 25 5.9
SPBP22H7.08 |rps1002|rps10-2, rps10B|40S ribosomal protein S10|S... 25 5.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 134 bits (324), Expect = 7e-33
Identities = 59/74 (79%), Positives = 67/74 (90%)
Frame = +1
Query: 256 LVDLEPGTMDSVRSGPFGQIFRPDIFVFGQSGAGNNWAKGHYTEGAELDDSVLDVVRKEA 435
LVDLEPGTMD+V+SG FG +FRPD ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+EA
Sbjct: 65 LVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREA 124
Query: 436 ESCDCLQGFQLTHS 477
E+CD LQGFQLTHS
Sbjct: 125 EACDALQGFQLTHS 138
Score = 112 bits (270), Expect = 2e-26
Identities = 47/63 (74%), Positives = 54/63 (85%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 244
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 245 PRA 253
PRA
Sbjct: 61 PRA 63
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 87.0 bits (206), Expect = 1e-18
Identities = 39/78 (50%), Positives = 55/78 (70%), Gaps = 1/78 (1%)
Frame = +1
Query: 247 PRHL-VDLEPGTMDSVRSGPFGQIFRPDIFVFGQSGAGNNWAKGHYTEGAELDDSVLDVV 423
PR + VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E+ DSVL+ +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 424 RKEAESCDCLQGFQLTHS 477
R+ A++C LQGF + HS
Sbjct: 127 RRMADNCSGLQGFLVFHS 144
Score = 49.6 bits (113), Expect = 2e-07
Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 6/69 (8%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 226
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 227 SGGKYVPRA 253
GK+VPR+
Sbjct: 61 GQGKFVPRS 69
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 85.4 bits (202), Expect = 4e-18
Identities = 38/78 (48%), Positives = 53/78 (67%), Gaps = 1/78 (1%)
Frame = +1
Query: 247 PRHL-VDLEPGTMDSVRSGPFGQIFRPDIFVFGQSGAGNNWAKGHYTEGAELDDSVLDVV 423
PR + VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G EL D V D +
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 424 RKEAESCDCLQGFQLTHS 477
R+ A++C LQGF + HS
Sbjct: 123 RRIADNCSGLQGFLVFHS 140
Score = 55.6 bits (128), Expect = 4e-09
Identities = 27/65 (41%), Positives = 37/65 (56%), Gaps = 2/65 (3%)
Frame = +2
Query: 65 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 238
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 239 YVPRA 253
YVPR+
Sbjct: 61 YVPRS 65
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 66.5 bits (155), Expect = 2e-12
Identities = 27/62 (43%), Positives = 43/62 (69%)
Frame = +2
Query: 68 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 247
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 248 RA 253
RA
Sbjct: 63 RA 64
Score = 64.5 bits (150), Expect = 8e-12
Identities = 27/76 (35%), Positives = 49/76 (64%), Gaps = 2/76 (2%)
Frame = +1
Query: 256 LVDLEPGTMDSVRSGPFGQIFRPDIFVFGQS--GAGNNWAKGHYTEGAELDDSVLDVVRK 429
L+DLEP ++++ S +G ++ P+ + ++ GAGNNWA G Y+ + + ++D++ +
Sbjct: 66 LIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDR 124
Query: 430 EAESCDCLQGFQLTHS 477
EA+ D L+GF L HS
Sbjct: 125 EADGSDSLEGFSLLHS 140
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 28.3 bits (60), Expect = 0.64
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +1
Query: 298 GPFGQIFRPDIFVFGQSGAG-NNWAKGHYTEGAELDDS 408
GP+G +F P F+F +G NW+ Y E A LD +
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHLDSN 193
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.5
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +2
Query: 104 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 205
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 25.4 bits (53), Expect = 4.5
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 182 SDLQLERINVYYNEASGGKYVPRAT 256
S L +E I+ Y A KY+PR+T
Sbjct: 635 SFLDIENIDEYLESAENMKYLPRST 659
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 25.0 bits (52), Expect = 5.9
Identities = 16/43 (37%), Positives = 20/43 (46%)
Frame = +1
Query: 232 RQVRAPRHLVDLEPGTMDSVRSGPFGQIFRPDIFVFGQSGAGN 360
R+V AP+H GT RS +FR + FGQ GN
Sbjct: 486 REVFAPKH-ARRRLGTRFHSRSSHRPSVFRDNSVAFGQLDNGN 527
>SPBP22H7.08 |rps1002|rps10-2, rps10B|40S ribosomal protein
S10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 25.0 bits (52), Expect = 5.9
Identities = 13/29 (44%), Positives = 15/29 (51%)
Frame = -1
Query: 316 RSVRKVQSGQSPWCRAPSRRGGAGHVLAA 230
R K G +P APS RGG G +AA
Sbjct: 119 RRAEKKDDGAAPGGFAPSFRGGFGRPVAA 147
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 24.6 bits (51), Expect = 7.9
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -3
Query: 356 PAPDCPKTKMSGRKICPKGPERTESMVPGSKS 261
P+ PK + R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,956,707
Number of Sequences: 5004
Number of extensions: 37432
Number of successful extensions: 121
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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