Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0293
(637 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 95 3e-20
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 94 8e-20
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 92 2e-19
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 60 9e-10
At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical t... 54 8e-08
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 48 5e-06
At1g12550.1 68414.m01455 oxidoreductase family protein similar t... 46 2e-05
At2g45630.2 68415.m05674 oxidoreductase family protein low simil... 45 5e-05
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 31 0.85
At4g35240.1 68417.m05009 expressed protein contains Pfam domains... 30 1.1
At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) simil... 30 1.5
At5g43260.1 68418.m05286 chaperone protein dnaJ-related similar ... 28 4.5
At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 d... 28 4.5
At4g11420.1 68417.m01840 eukaryotic translation initiation facto... 28 6.0
At1g06460.1 68414.m00684 31.2 kDa small heat shock family protei... 27 7.9
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 95.1 bits (226), Expect = 3e-20
Identities = 43/102 (42%), Positives = 63/102 (61%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWDRALYTG 477
DN+D+ +A + G V+NAP AN ++A E L+ +AR+V A ++KAG+W+R+ Y G
Sbjct: 157 DNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVG 216
Query: 478 SELAGKTLAILALAXXGREVATRMYAFGMNIIGFDPFVSADQ 603
L GKTLA++ G EVA R GM +I DP+ AD+
Sbjct: 217 VSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADR 258
Score = 38.7 bits (86), Expect = 0.003
Identities = 20/59 (33%), Positives = 34/59 (57%)
Frame = +2
Query: 83 VLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKEVLDEA 259
+L+ + +G LL +G + +S E+L ++ DAL+VRS T+VT+EV + A
Sbjct: 85 ILVTEKLGEAGVNLLREFGDVDCSY-DLSPEDLKKKVAESDALIVRSGTKVTREVFEAA 142
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 93.9 bits (223), Expect = 8e-20
Identities = 45/102 (44%), Positives = 60/102 (58%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWDRALYTG 477
DN+D+ +A + G V+NAP AN ++A E LM +AR+V A ++KAG W R Y G
Sbjct: 136 DNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKAGEWKRNKYVG 195
Query: 478 SELAGKTLAILALAXXGREVATRMYAFGMNIIGFDPFVSADQ 603
L GKTLA+L G EVA R GM +I DP+ AD+
Sbjct: 196 VSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADR 237
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/32 (43%), Positives = 23/32 (71%)
Frame = +2
Query: 164 ISKEELLMEIPNHDALVVRSATQVTKEVLDEA 259
++ EEL ++I DAL+VRS T+V +EV + +
Sbjct: 90 MTPEELNIKISLCDALIVRSGTKVGREVFESS 121
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 92.3 bits (219), Expect = 2e-19
Identities = 43/102 (42%), Positives = 62/102 (60%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWDRALYTG 477
DN+D+ +A + G V+NAP AN ++A E L+ +AR++ A ++KAG+W R Y G
Sbjct: 121 DNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYVG 180
Query: 478 SELAGKTLAILALAXXGREVATRMYAFGMNIIGFDPFVSADQ 603
L GKTLA+L G EVA R GM++I DP+ AD+
Sbjct: 181 VSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADR 222
Score = 33.5 bits (73), Expect = 0.12
Identities = 19/60 (31%), Positives = 34/60 (56%)
Frame = +2
Query: 80 SVLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKEVLDEA 259
++L+ + +G +LL Y + +S EEL +I DAL+VRS T+V ++V + +
Sbjct: 48 TILVTEKLGQAGIDLLKKYANVDCSY-DLSLEELCTKISLCDALIVRSGTKVGRDVFESS 106
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 60.5 bits (140), Expect = 9e-10
Identities = 35/103 (33%), Positives = 53/103 (51%), Gaps = 4/103 (3%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAG---RWDRAL 468
+N+DV++A K G+ V N PG + EL +L L AR +V A ++ G W L
Sbjct: 98 NNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHL 157
Query: 469 YTGSELAGKTLAILALAXXGREVATRMY-AFGMNIIGFDPFVS 594
+ G+ L G+T+ ++ G A M F MN+I FD + S
Sbjct: 158 FVGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQS 200
>At5g14780.1 68418.m01734 formate dehydrogenase (FDH) identical to
GI:7677266
Length = 384
Score = 54.0 bits (124), Expect = 8e-08
Identities = 29/97 (29%), Positives = 48/97 (49%), Gaps = 2/97 (2%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWDRA--LY 471
D+ID+ +A G+ V G+N +S E +L+L R+ VP + G W+ A Y
Sbjct: 131 DHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVVKGEWNVAGIAY 190
Query: 472 TGSELAGKTLAILALAXXGREVATRMYAFGMNIIGFD 582
+L GKT+ + G+ + R+ FG N++ D
Sbjct: 191 RAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHD 227
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 48.0 bits (109), Expect = 5e-06
Identities = 26/102 (25%), Positives = 47/102 (46%), Gaps = 1/102 (0%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWDRALY-T 474
D ID+ +KG+ V N P +L L+L L R + +++G+W + +
Sbjct: 79 DKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLCECDRYVRSGKWKQGEFQL 138
Query: 475 GSELAGKTLAILALAXXGREVATRMYAFGMNIIGFDPFVSAD 600
++ +GK++ I+ L G +A R AF I + + D
Sbjct: 139 TTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIKPD 180
>At1g12550.1 68414.m01455 oxidoreductase family protein similar to
glyoxylate reductase from Homo sapiens (gi:6002730);
contains Pfam D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain PF02826
Length = 323
Score = 46.4 bits (105), Expect = 2e-05
Identities = 24/90 (26%), Positives = 48/90 (53%), Gaps = 2/90 (2%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWDRA--LY 471
D+ID+ + ++G+ + NA A + + L++ + R + A +++G W +
Sbjct: 86 DHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIPAADRYVRSGNWAKFGDFQ 145
Query: 472 TGSELAGKTLAILALAXXGREVATRMYAFG 561
GS+++GK + I+ L G VA R+ +FG
Sbjct: 146 LGSKVSGKRVGIVGLGSIGSFVAKRLESFG 175
>At2g45630.2 68415.m05674 oxidoreductase family protein low
similarity to SP|P36234 Glycerate dehydrogenase (EC
1.1.1.29) (NADH-dependent hydroxypyruvate reductase)
{Hyphomicrobium methylovorum}; contains Pfam profile
PF00389: D-isomer specific 2-hydroxyacid dehydrogenase,
catalytic domain
Length = 338
Score = 44.8 bits (101), Expect = 5e-05
Identities = 27/93 (29%), Positives = 48/93 (51%), Gaps = 2/93 (2%)
Frame = +1
Query: 298 DNIDVDSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVPASTALKAGRWD-RALYT 474
D++D+ ++G+ V NA + + + L++ + R + A+ +K W + Y
Sbjct: 102 DHVDLVECRRRGISVANAGSSFSEDVADTAVGLLIDVFRRISAANRFVKQRFWPLKGDYP 161
Query: 475 -GSELAGKTLAILALAXXGREVATRMYAFGMNI 570
GS+L K + I+ L G +VATR+ AFG I
Sbjct: 162 LGSKLGRKRIGIVGLGSIGSKVATRLDAFGCQI 194
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 30.7 bits (66), Expect = 0.85
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
Frame = +1
Query: 82 GFDRRRGWRQVCRTPQR-LRNRHHHQGQDLQGRTSYGDTQPRRSGCAFSNSS 234
G R+ RQ +R L N+HH++ QD+Q + Y Q G + S SS
Sbjct: 1165 GAGRKSRQRQPDLMKKRMLLNKHHNRNQDVQQQNIYSPLQKTSKGPSLSKSS 1216
>At4g35240.1 68417.m05009 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 828
Score = 30.3 bits (65), Expect = 1.1
Identities = 14/31 (45%), Positives = 16/31 (51%)
Frame = -2
Query: 513 GQDGEGLASELAASVQSPVPPASLQRSGSWD 421
G G AS AA+ + P PP S RS WD
Sbjct: 249 GYYGSSSASTTAAATKPPPPPPSPPRSNGWD 279
>At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) similar
to glutamate decarboxylase (gad) GI:294111 from [Petunia
hybrida]
Length = 494
Score = 29.9 bits (64), Expect = 1.5
Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +2
Query: 59 KMVVDIKSVLI-VDGVGAKCAELLNAYGIATTTKAKISKEELLMEI 193
++V DI VL +D + +K ++ + GIA K K ++E+LME+
Sbjct: 431 RLVADISKVLHELDTLPSKISKKMGIEGIAENVKEKKMEKEILMEV 476
>At5g43260.1 68418.m05286 chaperone protein dnaJ-related similar to
Chaperone protein dnaJ (SP:Q9WZV3) [Thermotoga maritima]
Length = 97
Score = 28.3 bits (60), Expect = 4.5
Identities = 19/57 (33%), Positives = 21/57 (36%)
Frame = -2
Query: 621 CNGTGALISRHERVESDDVHAEGVHPSGHLAAXXCQGQDGEGLASELAASVQSPVPP 451
CNGTG + R DV SG A C G G G +VQ P P
Sbjct: 41 CNGTGRVTCFCSRWSDGDVGCRRCSGSGRAACSNC-GGSGTGRPLPAQITVQPPNRP 96
>At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3
domain-containing protein similar to eukaryotic protein
synthesis initiation factor [Homo sapiens] GI:3941724;
contains Pfam profiles PF02854: MIF4G domain, PF02847:
MA3 domain
Length = 1544
Score = 28.3 bits (60), Expect = 4.5
Identities = 21/83 (25%), Positives = 39/83 (46%), Gaps = 1/83 (1%)
Frame = -3
Query: 575 PMMFMPKAYILVATSRPXLAKARMARVLPASSLPVYRARSHLPAFSAVEAGTTW-RARTN 399
P + P A +++ KAR A+ + +S+LPV S PA + + G+ R+
Sbjct: 298 PQLIRPAAPAHLSSDSTSSVKARNAQNVMSSALPVNAKVSVKPAGVSEKLGSPKDRSHGE 357
Query: 398 INVQVSSHALSALAPGALITPTP 330
+N+ +S + A + + P P
Sbjct: 358 VNISLSQKNVEACSLSSSQQPKP 380
>At4g11420.1 68417.m01840 eukaryotic translation initiation factor 3
subunit 10 / eIF-3 theta / eIF3a (TIF3A1) identical to
eukaryotic translation initiation factor 3 subunit 10
(eIF-3 theta) (Eukaryotic translation initiation factor 3
large subunit) (eIF3a) (p114). [Arabidopsis thaliana]
SWISS-PROT:Q9LD55
Length = 987
Score = 27.9 bits (59), Expect = 6.0
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -1
Query: 460 GPTCQPSAQWKLGPRGEPEPTSTCRSARTR 371
GP + +W GPRG + ST S+R R
Sbjct: 954 GPPAEGGDRWGSGPRGSDDRRSTFGSSRPR 983
>At1g06460.1 68414.m00684 31.2 kDa small heat shock family protein /
hsp20 family protein contains Pfam profile: PF00011
Hsp20/alpha crystallin family
Length = 285
Score = 27.5 bits (58), Expect = 7.9
Identities = 18/67 (26%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = -3
Query: 539 ATSRPXLAK-ARMARVLPASSLPVYRARSHLPAFSAVEAGTTWRARTNINVQVSSHALSA 363
A + P +K A+ P+ S + + R L GT W R+N+ S+ ++
Sbjct: 142 AYAAPHFSKPAKEDIFFPSLSPNLQKERPKLDLPKLANLGTVWSPRSNVAESTHSYVVAI 201
Query: 362 LAPGALI 342
PGA I
Sbjct: 202 ELPGASI 208
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,780,207
Number of Sequences: 28952
Number of extensions: 233936
Number of successful extensions: 710
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 682
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 710
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -