Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0288
(730 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 56 7e-09
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 42 7e-05
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 39 8e-04
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 37 0.003
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 30 0.39
SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.7
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 27 3.6
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 8.4
SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizos... 25 8.4
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 55.6 bits (128), Expect = 7e-09
Identities = 26/87 (29%), Positives = 39/87 (44%), Gaps = 1/87 (1%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNEP 200
+HE C C I R+ +C CY++K+A +C KC K I V +
Sbjct: 337 FHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPILGISVKGSDGE 396
Query: 201 WHRECFTCTNCNTSLAGQGSLRATRSP 281
+H +C+TC CN L +G +P
Sbjct: 397 YHSQCWTCGACNALLGDEGYFMIENTP 423
Score = 50.4 bits (115), Expect = 3e-07
Identities = 22/73 (30%), Positives = 33/73 (45%)
Frame = +2
Query: 266 RDEKPYCAECFGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSLVGKG 445
RD+ +C C+ +A +C C KPI GI + D +H+ C+ C C L +G
Sbjct: 360 RDDLYWCQTCYDNKYAVKCKKCRKPILGIS----VKGSDGEYHSQCWTCGACNALLGDEG 415
Query: 446 FITDGQDIICPEC 484
+ IC C
Sbjct: 416 YFMIENTPICRPC 428
Score = 47.6 bits (108), Expect = 2e-06
Identities = 21/73 (28%), Positives = 32/73 (43%)
Frame = +3
Query: 18 RQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNE 197
++ H +CF C C + F RE + YC Y ++F+ RC C I V N+
Sbjct: 276 KKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINND 335
Query: 198 PWHRECFTCTNCN 236
+H C C+
Sbjct: 336 WFHENHHFCAGCS 348
Score = 31.5 bits (68), Expect = 0.13
Identities = 20/64 (31%), Positives = 29/64 (45%)
Frame = +2
Query: 230 LQHVARRPGFTSRDEKPYCAECFGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFI 409
L+HV GF R+ K YC + E F+ RC C PI + + + +H +
Sbjct: 292 LEHV----GFYYREGKFYCHLDYHEQFSPRCKHCKTPIE----DQAVHINNDWFHENHHF 343
Query: 410 CAQC 421
CA C
Sbjct: 344 CAGC 347
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 42.3 bits (95), Expect = 7e-05
Identities = 22/88 (25%), Positives = 33/88 (37%), Gaps = 3/88 (3%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFI---PREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKN 194
WH+ CFCC C + + + C+ C A C C I +
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIKDYALMSGY 94
Query: 195 EPWHRECFTCTNCNTSLAGQGSLRATRS 278
+ +HRECF C +C + R R+
Sbjct: 95 DSYHRECFRCHDCRKQIIDSNFKRDNRT 122
Score = 39.9 bits (89), Expect = 4e-04
Identities = 21/72 (29%), Positives = 32/72 (44%)
Frame = +2
Query: 269 DEKPYCAECFGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSLVGKGF 448
D +P C+ C A CT+C I +S D + H +CF C C+ ++ F
Sbjct: 66 DGRPVCSSC-----AHTCTACRMRIKDYA---LMSGYDSY-HRECFRCHDCRKQIIDSNF 116
Query: 449 ITDGQDIICPEC 484
D + I C +C
Sbjct: 117 KRDNRTIFCNDC 128
Score = 35.5 bits (78), Expect = 0.008
Identities = 15/59 (25%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +3
Query: 129 KFATRCVKCNKIIT--QGGVTYKNEPWHRECFTCTNCNTSLAGQGSLRATRSPTAPSAS 299
K +T C +C + + +++ WH++CF CT C+ L + S P S
Sbjct: 14 KGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCS 72
Score = 32.7 bits (71), Expect = 0.055
Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 4/44 (9%)
Frame = +2
Query: 368 ISFEDRHWHNDCFICAQCKTSLVGKGFI----TDGQDIICPECA 487
ISF WH DCF C +C L + +DG+ +C CA
Sbjct: 33 ISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRP-VCSSCA 75
Score = 28.3 bits (60), Expect = 1.2
Identities = 11/40 (27%), Positives = 20/40 (50%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATR 143
+H +CF C C+ I +F + I+C C + + +R
Sbjct: 97 YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 38.7 bits (86), Expect = 8e-04
Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Frame = +3
Query: 18 RQWHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGCYEDKFATRCVKCN-KIITQGGVTYK 191
+++H + F C +C G S+ E ++YC Y FA RC C+ I+ Q Y+
Sbjct: 197 KKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYR 256
Query: 192 N---EPWHREC 215
N + WH C
Sbjct: 257 NGVSQNWHVPC 267
Score = 35.9 bits (79), Expect = 0.006
Identities = 21/77 (27%), Positives = 33/77 (42%), Gaps = 5/77 (6%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSF-----IPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTY 188
+H +CF C C + + +K F +Q C Y + C C + +T
Sbjct: 135 YHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCGMALRGYYITA 194
Query: 189 KNEPWHRECFTCTNCNT 239
N+ +H E FTC+ C T
Sbjct: 195 LNKKFHIEHFTCSLCYT 211
Score = 32.7 bits (71), Expect = 0.055
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +3
Query: 144 CVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSLAGQ 254
C C ++I+ V +H ECF C +CN+ +A +
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASK 152
Score = 29.5 bits (63), Expect = 0.51
Identities = 14/43 (32%), Positives = 19/43 (44%)
Frame = +2
Query: 275 KPYCAECFGELFAKRCTSCTKPITGIGGTRFISFEDRHWHNDC 403
K YC + LFA RC C PI + + ++WH C
Sbjct: 225 KVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 36.7 bits (81), Expect = 0.003
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 144 CVKC-NKIITQGGVTYKNEPWHRECFTCTNCNTSL 245
C+KC + + V + + WH +CF C NCN L
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKL 58
Score = 35.1 bits (77), Expect = 0.010
Identities = 19/61 (31%), Positives = 26/61 (42%), Gaps = 3/61 (4%)
Frame = +2
Query: 311 AKRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSL--VGKGFITDGQ-DIICPE 481
++R C K + T + F + WH+DCF C C L + F D Q I C
Sbjct: 18 SERTCFCIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKL 77
Query: 482 C 484
C
Sbjct: 78 C 78
Score = 29.5 bits (63), Expect = 0.51
Identities = 18/70 (25%), Positives = 29/70 (41%), Gaps = 3/70 (4%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPI--GTKSFIPREQ-EIYCAGCYEDKFATRCVKCNKIITQGGVTYKN 194
WH CF CV C + ++ F +Q +I+C C + C C+ I + +
Sbjct: 44 WHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD-----ICNGCSTPICEFNAVSNS 98
Query: 195 EPWHRECFTC 224
+ H C C
Sbjct: 99 QANHPCCSNC 108
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 29.9 bits (64), Expect = 0.39
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2
Query: 386 HWHNDCFICAQCKTSLVGKGFI 451
H + C++CA+CKT L KG +
Sbjct: 8 HLKSRCYVCAKCKTHLAFKGHL 29
>SPBC1E8.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 317
Score = 27.1 bits (57), Expect = 2.7
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = +3
Query: 174 GGVTYKNEPWHRECFTCTNCNTSLAGQGSLRATRSPTAPSASG 302
GG TY + C TCT +SL+ G++ +T APS G
Sbjct: 98 GGETYSQYFTLQACSTCTISTSSLSYSGTISST--SIAPSMIG 138
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 26.6 bits (56), Expect = 3.6
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = -2
Query: 444 PLPTSEVLHCAQMKQSLCQ-CLSSNEMKRVPPIPVMGLVQEVQRLANSSPKHSAQ*GFSS 268
P P+SEV C +Q + + L +N + P +Q RL N + + F +
Sbjct: 357 PFPSSEVFRCLATRQEILKGMLGANILGFQIPEFAYHFLQTCSRLVNIDIRKNGVVSFEN 416
Query: 267 REVN 256
R+++
Sbjct: 417 RQID 420
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 25.4 bits (53), Expect = 8.4
Identities = 9/18 (50%), Positives = 14/18 (77%), Gaps = 1/18 (5%)
Frame = -3
Query: 587 KHLSYINCIACE-GWSDA 537
KHL+++NCI C W++A
Sbjct: 209 KHLAHVNCINCNTPWANA 226
>SPCC1020.10 |oca2||serine/threonine protein kinase Oca2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 650
Score = 25.4 bits (53), Expect = 8.4
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +1
Query: 421 QDLARRQGLHHRRTGHH 471
Q + R+ GLHH + GHH
Sbjct: 213 QFIFRKLGLHHGKHGHH 229
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,467,021
Number of Sequences: 5004
Number of extensions: 48524
Number of successful extensions: 159
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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