BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0288
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g17890.1 68418.m02098 LIM domain-containing protein / disease... 46 3e-05
At4g36860.1 68417.m05226 LIM domain-containing protein low simil... 42 4e-04
At3g55770.1 68416.m06197 LIM domain-containing protein similar t... 32 0.002
At1g19270.1 68414.m02397 ubiquitin interaction motif-containing ... 40 0.002
At2g45800.1 68415.m05696 LIM domain-containing protein similar t... 31 0.003
At5g66610.1 68418.m08396 LIM domain-containing protein contains ... 38 0.007
At3g61230.1 68416.m06852 LIM domain-containing protein similar t... 34 0.084
At5g66620.1 68418.m08397 LIM domain-containing protein contains ... 33 0.19
At5g66630.1 68418.m08398 LIM domain-containing protein contains ... 31 1.0
At1g01780.1 68414.m00097 LIM domain-containing protein similar t... 31 1.0
At5g22760.1 68418.m02658 PHD finger family protein contains Pfam... 30 1.4
At3g23060.1 68416.m02907 zinc finger (C3HC4-type RING finger) fa... 30 1.8
At2g39900.1 68415.m04904 LIM domain-containing protein similar t... 30 1.8
At1g10200.1 68414.m01150 transcription factor LIM, putative stro... 29 2.4
At4g32960.1 68417.m04689 expressed protein 29 3.2
At1g77020.1 68414.m08969 DNAJ heat shock N-terminal domain-conta... 29 4.2
At1g36150.1 68414.m04494 protease inhibitor/seed storage/lipid t... 28 5.5
At1g05630.1 68414.m00584 endonuclease/exonuclease/phosphatase fa... 28 7.3
>At5g17890.1 68418.m02098 LIM domain-containing protein / disease
resistance protein-related low similarity to disease
resistance protein RPP4 [Arabidopsis thaliana]
GI:20270890; contains Pfam profiles PF00412: LIM domain,
PF00931: NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1613
Score = 46.0 bits (104), Expect = 3e-05
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 2/67 (2%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKCNKII--TQGGVTYKNE 197
WH +CFCC+ C+ PI I + +Y CY++ C C K I T G+ Y
Sbjct: 1260 WHPQCFCCLRCREPIAMNE-ISDLRGMYHKPCYKELRHPNCYVCEKKIPRTAEGLKYHEH 1318
Query: 198 PWHRECF 218
P+ E +
Sbjct: 1319 PFWMETY 1325
Score = 30.7 bits (66), Expect = 1.0
Identities = 11/38 (28%), Positives = 16/38 (42%), Gaps = 1/38 (2%)
Frame = +3
Query: 138 TRCVKCNKIITQG-GVTYKNEPWHRECFTCTNCNTSLA 248
++C C I G + WH +CF C C +A
Sbjct: 1238 SKCKDCKSAIEDGISINAYGSVWHPQCFCCLRCREPIA 1275
>At4g36860.1 68417.m05226 LIM domain-containing protein low
similarity to LIM-domain protein [Branchiostoma
floridae] GI:3360516, DRAL/Slim3/FHL2 [Homo sapiens]
GI:7209525; contains Pfam profile PF00412: LIM domain
Length = 351
Score = 41.9 bits (94), Expect = 4e-04
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKCNKII---TQGGVTYKN 194
WH +CFCC C PI F Y CY+++ +C C+ I G + Y+
Sbjct: 5 WHPECFCCNACDKPIIDYEFSMSGNRPYHKLCYKEQHHPKCDVCHNFIPTNPAGLIEYRA 64
Query: 195 EPW 203
P+
Sbjct: 65 HPF 67
>At3g55770.1 68416.m06197 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 199
Score = 31.9 bits (69), Expect(2) = 0.002
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF 134
+H+ CF C CK+ + S+ E +YC +E F
Sbjct: 30 YHKSCFKCTHCKSRLQLSSYSSMEGVLYCKPHFEQLF 66
Score = 29.1 bits (62), Expect = 3.2
Identities = 11/40 (27%), Positives = 20/40 (50%)
Frame = +2
Query: 314 KRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSL 433
++C +C K + + +S + +H CF C CK+ L
Sbjct: 8 QKCKACEKTVYAV---ELLSADGVGYHKSCFKCTHCKSRL 44
Score = 27.1 bits (57), Expect(2) = 0.002
Identities = 9/31 (29%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = +3
Query: 141 RCVKCNKIITQ-GGVTYKNEPWHRECFTCTN 230
+C C+K + VT +++ +H+ CF C++
Sbjct: 107 KCATCSKTVYPIEKVTVESQTYHKSCFKCSH 137
>At1g19270.1 68414.m02397 ubiquitin interaction motif-containing
protein / LIM domain-containing protein weak similarity
to LIM-homeobox protein [Mus musculus] GI:2149584, Hic-5
[Mus musculus] GI:664955; contains Pfam profiles
PF02809: Ubiquitin interaction motif, PF00412: LIM
domain
Length = 532
Score = 39.5 bits (88), Expect = 0.002
Identities = 17/63 (26%), Positives = 27/63 (42%), Gaps = 3/63 (4%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKCNKIIT---QGGVTYKN 194
WH +CF C C PI F + CY +++ +C C+ I G + Y+
Sbjct: 192 WHPECFRCYGCSQPISEYEFSTSGNYPFHKACYRERYHPKCDVCSHFIPTNHAGLIEYRA 251
Query: 195 EPW 203
P+
Sbjct: 252 HPF 254
Score = 32.3 bits (70), Expect = 0.34
Identities = 22/101 (21%), Positives = 38/101 (37%), Gaps = 1/101 (0%)
Frame = +2
Query: 320 CTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSLVGKGFITDGQDIICPECAKQKL 499
C C I G RF++ + WH +CF C C + F T G C +++
Sbjct: 172 CAGCNMEI---GHGRFLNCLNSLWHPECFRCYGCSQPISEYEFSTSGNYPFHKACYRERY 228
Query: 500 M*ITCLAS-TLPLSHRSIPHTQYNLYMTNVFVMCSEHTILP 619
+ S +P +H + + + + + EH P
Sbjct: 229 HPKCDVCSHFIPTNHAGLIEYRAHPFWVQKYCPSHEHDATP 269
Score = 31.1 bits (67), Expect = 0.78
Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 1/36 (2%)
Frame = +3
Query: 144 CVKCNKIITQGG-VTYKNEPWHRECFTCTNCNTSLA 248
C CN I G + N WH ECF C C+ ++
Sbjct: 172 CAGCNMEIGHGRFLNCLNSLWHPECFRCYGCSQPIS 207
>At2g45800.1 68415.m05696 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 226
Score = 31.1 bits (67), Expect = 0.78
Identities = 11/36 (30%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +3
Query: 141 RCVKCNKII-TQGGVTYKNEPWHRECFTCTNCNTSL 245
+C C+K + +T + +H+ CF CT+C +L
Sbjct: 9 KCKACDKTVYVMDLLTLEGNTYHKSCFRCTHCKGTL 44
Score = 29.5 bits (63), Expect = 2.4
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +2
Query: 317 RCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSLV 436
+C +C K + + ++ E +H CF C CK +LV
Sbjct: 9 KCKACDKTVYVMD---LLTLEGNTYHKSCFRCTHCKGTLV 45
Score = 29.1 bits (62), Expect(2) = 0.003
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF 134
+H+ CF C CK + ++ + +YC +E F
Sbjct: 30 YHKSCFRCTHCKGTLVISNYSSMDGVLYCKPHFEQLF 66
Score = 29.1 bits (62), Expect(2) = 0.003
Identities = 12/36 (33%), Positives = 18/36 (50%), Gaps = 1/36 (2%)
Frame = +3
Query: 141 RCVKCNKIITQ-GGVTYKNEPWHRECFTCTNCNTSL 245
+C C K + VT + E +H+ CF CT+ L
Sbjct: 105 KCATCKKTVYPLEKVTMEGESYHKTCFRCTHSGCPL 140
>At5g66610.1 68418.m08396 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 529
Score = 37.9 bits (84), Expect = 0.007
Identities = 22/74 (29%), Positives = 30/74 (40%), Gaps = 4/74 (5%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSF--IPREQEIYCAGCYEDKFATRCVKCNKIITQGG--VTYK 191
WH +CFCC C PI + P +E YC +E +C C ++ G V
Sbjct: 221 WHPECFCCRYCDKPIAMHEYKEHPFWKEKYCP-FHEVDGTPKCCSCERLEPWGTKYVMLA 279
Query: 192 NEPWHRECFTCTNC 233
+ W C C C
Sbjct: 280 DNRW--LCVKCMEC 291
Score = 27.9 bits (59), Expect = 7.3
Identities = 13/36 (36%), Positives = 15/36 (41%), Gaps = 1/36 (2%)
Frame = +3
Query: 144 CVKCNKIITQG-GVTYKNEPWHRECFTCTNCNTSLA 248
C C I G V WH ECF C C+ +A
Sbjct: 201 CDGCKSAIEYGRSVHALGVNWHPECFCCRYCDKPIA 236
>At3g61230.1 68416.m06852 LIM domain-containing protein similar to
SP|P29675 Pollen specific protein SF3 {Helianthus
annuus}; contains Pfam profile PF00412: LIM domain
Length = 213
Score = 34.3 bits (75), Expect = 0.084
Identities = 12/36 (33%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = +3
Query: 141 RCVKCNKII-TQGGVTYKNEPWHRECFTCTNCNTSL 245
+C C+K + +T + P+H+ CF C++CN +L
Sbjct: 10 KCKACDKTVYVMDLMTLEGMPYHKSCFRCSHCNGTL 45
Score = 25.8 bits (54), Expect(2) = 0.38
Identities = 10/36 (27%), Positives = 17/36 (47%), Gaps = 1/36 (2%)
Frame = +3
Query: 141 RCVKCNKIITQ-GGVTYKNEPWHRECFTCTNCNTSL 245
+C C K + +T + E +H+ CF C + L
Sbjct: 106 KCAACKKTVYPLEKMTMEGESYHKTCFRCAHSGCPL 141
Score = 25.0 bits (52), Expect(2) = 0.38
Identities = 9/37 (24%), Positives = 17/37 (45%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF 134
+H+ CF C C + ++ + +YC +E F
Sbjct: 31 YHKSCFRCSHCNGTLVICNYSSMDGVLYCKTHFEQLF 67
>At5g66620.1 68418.m08397 LIM domain-containing protein contains
Pfam profile PF00412: LIM domain
Length = 644
Score = 33.1 bits (72), Expect = 0.19
Identities = 19/66 (28%), Positives = 27/66 (40%), Gaps = 3/66 (4%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIG---TKSFIPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKN 194
WH CFCC C PI ++ + + + CYE C C + + TY N
Sbjct: 306 WHPGCFCCRACHKPIAIHDIENHVSNSRGKFHKSCYE----RYCYVCKE---KKMKTYNN 358
Query: 195 EPWHRE 212
P+ E
Sbjct: 359 HPFWEE 364
Score = 29.5 bits (63), Expect = 2.4
Identities = 13/36 (36%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +3
Query: 144 CVKCNKIITQGG-VTYKNEPWHRECFTCTNCNTSLA 248
C CN + GG V WH CF C C+ +A
Sbjct: 286 CGGCNFAVEHGGSVNILGVLWHPGCFCCRACHKPIA 321
>At5g66630.1 68418.m08398 LIM domain-containing protein contains low
similarity to Pfam profile PF00412: LIM domain
Length = 702
Score = 30.7 bits (66), Expect = 1.0
Identities = 12/37 (32%), Positives = 17/37 (45%), Gaps = 3/37 (8%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIG---TKSFIPREQEIYCAGCYE 125
WH CFCC C PI ++ + + + CYE
Sbjct: 367 WHPGCFCCRSCDKPIAIHELENHVSNSRGKFHKSCYE 403
>At1g01780.1 68414.m00097 LIM domain-containing protein similar to
PGPS/D1 [Petunia x hybrida] GI:4105772, LIM domain
protein PLIM1 [Nicotiana tabacum] GI:5932418; contains
Pfam profile PF00412: LIM domain
Length = 205
Score = 30.7 bits (66), Expect = 1.0
Identities = 11/36 (30%), Positives = 21/36 (58%), Gaps = 1/36 (2%)
Frame = +3
Query: 141 RCVKCNKII-TQGGVTYKNEPWHRECFTCTNCNTSL 245
+C C+K + ++ + P+H+ CF CT+C +L
Sbjct: 9 KCNVCDKTVYVVDMLSIEGMPYHKSCFRCTHCKGTL 44
Score = 29.5 bits (63), Expect = 2.4
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF 134
+H+ CF C CK + ++ + +YC +E F
Sbjct: 30 YHKSCFRCTHCKGTLQMSNYSSMDGVLYCKTHFEQLF 66
Score = 28.7 bits (61), Expect = 4.2
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = +2
Query: 317 RCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSL 433
+C C K + + +S E +H CF C CK +L
Sbjct: 9 KCNVCDKTVYVVD---MLSIEGMPYHKSCFRCTHCKGTL 44
>At5g22760.1 68418.m02658 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 1566
Score = 30.3 bits (65), Expect = 1.4
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = -3
Query: 620 PVVWYVRCTSQKHLSYINCIACEGWSDARAEALKRDRL 507
PV W + L+YI+C +C+ W A L+ ++
Sbjct: 1243 PVCWICKLPYNPGLTYIHCTSCDMWYHIEAVKLEESKI 1280
>At3g23060.1 68416.m02907 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 480
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/45 (28%), Positives = 17/45 (37%), Gaps = 4/45 (8%)
Frame = +3
Query: 36 CFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF----ATRCVKCN 158
C C +C NP + I +C C +KF C CN
Sbjct: 13 CLACPICTNPFKDATTISECLHTFCRSCIRNKFINERVNACPVCN 57
>At2g39900.1 68415.m04904 LIM domain-containing protein similar to
pollen specific LIM domain protein 1b [Nicotiana
tabacum] GI:6467905, PGPS/D1 [Petunia x hybrida]
GI:4105772; contains Pfam profile PF00412: LIM domain
Length = 200
Score = 29.9 bits (64), Expect = 1.8
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = +3
Query: 24 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF 134
+H+ CF C CK+ + ++ E +YC +E F
Sbjct: 30 YHKACFKCSHCKSRLQLSNYSSMEGVVYCRPHFEQLF 66
Score = 28.3 bits (60), Expect = 5.5
Identities = 11/40 (27%), Positives = 21/40 (52%)
Frame = +2
Query: 314 KRCTSCTKPITGIGGTRFISFEDRHWHNDCFICAQCKTSL 433
++C +C K + + +S + +H CF C+ CK+ L
Sbjct: 8 QKCRACEKTVYPV---ELLSADGISYHKACFKCSHCKSRL 44
>At1g10200.1 68414.m01150 transcription factor LIM, putative strong
similarity to transcription factor Ntlim1 [Nicotiana
tabacum] GI:5689136, LIM domain protein WLIM-1
[Helianthus annuus] GI:5070280; contains Pfam profile
PF00412: LIM domain
Length = 190
Score = 29.5 bits (63), Expect = 2.4
Identities = 11/36 (30%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +3
Query: 141 RCVKCNKII-TQGGVTYKNEPWHRECFTCTNCNTSL 245
+C+ C+K + +T N +H+ CF C +C +L
Sbjct: 9 KCMACDKTVYLVDKLTADNRVYHKACFRCHHCKGTL 44
Score = 28.7 bits (61), Expect = 4.2
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = +3
Query: 18 RQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKF 134
R +H+ CF C CK + ++ E +YC ++ F
Sbjct: 28 RVYHKACFRCHHCKGTLKLSNYNSFEGVLYCRPHFDQNF 66
>At4g32960.1 68417.m04689 expressed protein
Length = 264
Score = 29.1 bits (62), Expect = 3.2
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
Frame = +2
Query: 341 ITGIGGTRFISFEDRHWHNDCFIC--AQCKTSLVGKGFITDGQDIICPECAKQKLM 502
+TG+G TR+ R D IC ++ + K T+G+ +I EC KQ ++
Sbjct: 8 VTGVGTTRYALKPGRIKSEDILICIDVDAESMVEMKTTGTNGRPLIRMECVKQAII 63
>At1g77020.1 68414.m08969 DNAJ heat shock N-terminal
domain-containing protein similar to SP|P39101 CAJ1
protein [Saccharomyces cerevisiae]; contains Pfam
profile PF00226 DnaJ domain
Length = 379
Score = 28.7 bits (61), Expect = 4.2
Identities = 15/46 (32%), Positives = 26/46 (56%)
Frame = +2
Query: 137 HPLRQV*QDNHTRRRDVQERAMAPRVLHVHELQHVARRPGFTSRDE 274
H + V ++N+ R+ +++ RAMA ++L Q + G TSR E
Sbjct: 280 HVCQMVFRENNLRKEELKSRAMALKILGKIFQQEKQSKNGTTSRKE 325
>At1g36150.1 68414.m04494 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein low similarity to
glucoamylase S1/S2 [Precursor] from Saccharomyces
cerevisiae [SP|P08640], proteophosphoglycan from
Leishmania major [GI:5420387]; contains Pfam protease
inhibitor/seed storage/LTP family domain PF00234
Length = 256
Score = 28.3 bits (60), Expect = 5.5
Identities = 22/70 (31%), Positives = 29/70 (41%), Gaps = 1/70 (1%)
Frame = -2
Query: 465 SCPSVMKPLPT-SEVLHCAQMKQSLCQCLSSNEMKRVPPIPVMGLVQEVQRLANSSPKHS 289
S P M P P S C+ + S+ CLS + P P V+ + N SPK
Sbjct: 28 SQPPSMAPTPQPSNSTDCSSVIYSMVDCLSFLTVGSTDPSPTKTCCVGVKTVLNYSPKCL 87
Query: 288 AQ*GFSSREV 259
SSRE+
Sbjct: 88 CSALESSREM 97
>At1g05630.1 68414.m00584 endonuclease/exonuclease/phosphatase
family protein similar to inositol 1,4,5-trisphosphate
5-phosphatase (GI:4688596) [Arabidopsis thaliana]
Length = 861
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +1
Query: 316 ALHFLHQAHHRYRRHPLHLV-RRQTLAQRLLHLRAVQD 426
+L F H+ HH+ R+H L V R TLA ++ + D
Sbjct: 30 SLQFDHKPHHQIRKHSLDEVPRSATLASEAVYFDSSDD 67
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,382,726
Number of Sequences: 28952
Number of extensions: 271682
Number of successful extensions: 890
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 815
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 890
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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