BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0283 (497 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 103 1e-23 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 103 1e-23 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 63 2e-11 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 44 2e-05 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 40 2e-04 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 35 0.008 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.014 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.014 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.014 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 32 0.055 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 29 0.39 SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 28 0.90 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 28 0.90 SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 27 2.1 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 26 2.7 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 2.7 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 26 2.7 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 3.6 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 4.8 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 4.8 SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 25 4.8 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 6.3 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 25 6.3 SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 25 6.3 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 8.4 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 103 bits (248), Expect = 1e-23 Identities = 48/66 (72%), Positives = 55/66 (83%) Frame = +2 Query: 53 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTRFTDTR 232 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AGD RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 233 KDEQDR 250 DEQ+R Sbjct: 61 ADEQER 66 Score = 74.1 bits (174), Expect = 1e-14 Identities = 44/81 (54%), Positives = 51/81 (62%), Gaps = 1/81 (1%) Frame = +1 Query: 256 TIKSTAISMFFELEEKDLVFITNPDQREKSD-KGFLINLIASPGHVDFSSEVTAALRVTX 432 TIKSTAIS+F E+ + D+ D +E +D FL+NLI SPGHVDFSSEVTAALRVT Sbjct: 69 TIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTD 123 Query: 433 XXXXXXXXXXXXXXQTETVLR 495 QTETVLR Sbjct: 124 GALVVVDTIEGVCVQTETVLR 144 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 103 bits (248), Expect = 1e-23 Identities = 48/66 (72%), Positives = 55/66 (83%) Frame = +2 Query: 53 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTRFTDTR 232 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AGD RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 233 KDEQDR 250 DEQ+R Sbjct: 61 ADEQER 66 Score = 74.1 bits (174), Expect = 1e-14 Identities = 44/81 (54%), Positives = 51/81 (62%), Gaps = 1/81 (1%) Frame = +1 Query: 256 TIKSTAISMFFELEEKDLVFITNPDQREKSD-KGFLINLIASPGHVDFSSEVTAALRVTX 432 TIKSTAIS+F E+ + D+ D +E +D FL+NLI SPGHVDFSSEVTAALRVT Sbjct: 69 TIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTD 123 Query: 433 XXXXXXXXXXXXXXQTETVLR 495 QTETVLR Sbjct: 124 GALVVVDTIEGVCVQTETVLR 144 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 63.3 bits (147), Expect = 2e-11 Identities = 28/60 (46%), Positives = 41/60 (68%) Frame = +2 Query: 71 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTRFTDTRKDEQDR 250 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Score = 56.0 bits (129), Expect = 3e-09 Identities = 35/80 (43%), Positives = 49/80 (61%) Frame = +1 Query: 256 TIKSTAISMFFELEEKDLVFITNPDQREKSDKGFLINLIASPGHVDFSSEVTAALRVTXX 435 T+KS+AIS+FF++ I+ D++ + +K +LINLI SPGHVDFSSEV++A R+ Sbjct: 69 TMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDG 120 Query: 436 XXXXXXXXXXXXXQTETVLR 495 QT TVLR Sbjct: 121 AFVLVDAVEGVCSQTITVLR 140 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 43.6 bits (98), Expect = 2e-05 Identities = 25/70 (35%), Positives = 40/70 (57%) Frame = +2 Query: 77 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTRFTDTRKDEQDRCT 256 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D K E +R Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLD--KLEVERRR 104 Query: 257 PLNLRPSLCS 286 + ++ CS Sbjct: 105 GITVKAQTCS 114 Score = 32.7 bits (71), Expect = 0.032 Identities = 13/24 (54%), Positives = 19/24 (79%) Frame = +1 Query: 349 KGFLINLIASPGHVDFSSEVTAAL 420 + +L+NLI +PGHVDF +EV +L Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSL 145 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 39.9 bits (89), Expect = 2e-04 Identities = 22/58 (37%), Positives = 29/58 (50%) Frame = +1 Query: 322 NPDQREKSDKGFLINLIASPGHVDFSSEVTAALRVTXXXXXXXXXXXXXXXQTETVLR 495 N Q+ +K + IN+I +PGH+DF+ EV ALRV QT TV R Sbjct: 134 NEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDR 191 Score = 35.1 bits (77), Expect = 0.006 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +2 Query: 59 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 190 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 34.7 bits (76), Expect = 0.008 Identities = 13/27 (48%), Positives = 21/27 (77%) Frame = +2 Query: 104 NIRNMSVIAHVDHGKSTLTDSLVSKAG 184 +IRN+ +IAH+D GK+TLT+ ++ G Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGG 53 Score = 28.7 bits (61), Expect = 0.51 Identities = 12/22 (54%), Positives = 16/22 (72%) Frame = +1 Query: 361 INLIASPGHVDFSSEVTAALRV 426 INLI +PGH DF+ EV ++ V Sbjct: 95 INLIDTPGHADFTFEVERSVAV 116 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.014 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 89 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 184 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.014 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 89 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 184 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.014 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 89 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 184 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 31.9 bits (69), Expect = 0.055 Identities = 18/52 (34%), Positives = 27/52 (51%) Frame = +2 Query: 95 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTRFTDTRKDEQDR 250 KK ++ N+ I HVDHGK+TLT ++ + A D D +E+ R Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKAR 100 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 29.1 bits (62), Expect = 0.39 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +1 Query: 349 KGFLINLIASPGHVDFSSEVTAALRVT 429 K F I +PGHVDF EV A + ++ Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAIS 233 >SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 794 Score = 27.9 bits (59), Expect = 0.90 Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 1/44 (2%) Frame = +2 Query: 230 RKDEQDRCTPLNLRPSLCSSSLKRKI-*YSSQTLTSVKRVIKVS 358 +KD R +PLN + + S +K+K+ ++S T TS+++ I S Sbjct: 424 QKDSMRRSSPLNEKVTASSERMKKKLALFASSTDTSMQKTIDSS 467 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.9 bits (59), Expect = 0.90 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +2 Query: 116 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTR 217 ++++ HVDHGK+TL D+ K+ I + G T+ Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQ 206 >SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 26.6 bits (56), Expect = 2.1 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = -1 Query: 455 QSTTTRDPSVTRSAAVTSEEKSTCPGEAIKLIKKPLSL 342 QSTT ++ S+ +A +S E ST G + PL++ Sbjct: 336 QSTTNKNDSLRNTAVESSTEPSTSNGFPATSVSPPLTI 373 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 26.2 bits (55), Expect = 2.7 Identities = 20/70 (28%), Positives = 32/70 (45%) Frame = -1 Query: 491 STVSVCTHTPDTQSTTTRDPSVTRSAAVTSEEKSTCPGEAIKLIKKPLSLFSRWSGFVMN 312 S+ S T TP + STT+ S + S ++S S+ A + S S S + Sbjct: 140 SSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSS-SASSSGSISSAD 198 Query: 311 TKSFSSSSKN 282 K+ S+SS + Sbjct: 199 AKTVSASSNS 208 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 2.7 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 113 NMSVIAHVDHGKSTLTDSLVSKAGII 190 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 26.2 bits (55), Expect = 2.7 Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 1/37 (2%) Frame = +3 Query: 138 ITASQPSRTRWFPR-PVSLLVREPETPVSLTRVRTNK 245 +T S T + P P S + REP +P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.8 bits (54), Expect = 3.6 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = -1 Query: 353 PLSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLM 258 P S +S W+ V+NTK SFS ++M +L+ Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELI 199 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 4.8 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -2 Query: 109 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 14 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 4.8 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -1 Query: 104 SASCPSSHGSRLP*NSPF*MVYDFCSIK 21 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPBC25H2.15 |||programmed cell death protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 25.4 bits (53), Expect = 4.8 Identities = 18/92 (19%), Positives = 40/92 (43%) Frame = +2 Query: 107 IRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGDTRFTDTRKDEQDRCTPLNLRPSLCS 286 +++ I+H++ KS+ + V+ I + + G F+D ++ PS S Sbjct: 112 VKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDATSANNPFSLSTDVNPSKPS 171 Query: 287 SSLKRKI*YSSQTLTSVKRVIKVS*ST*LPHL 382 S++ K ++++ S+ K T H+ Sbjct: 172 SNVFSKPSFAAKAQQSITDQQKTQAKTKTKHI 203 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.0 bits (52), Expect = 6.3 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +2 Query: 113 NMSVIAHVDHGKSTLTDSL 169 N+ I HV HGKST+ ++ Sbjct: 25 NIGTIGHVAHGKSTVVKAI 43 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.0 bits (52), Expect = 6.3 Identities = 17/53 (32%), Positives = 28/53 (52%) Frame = +2 Query: 32 KNHKPSKMVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII 190 K P+ +V+ V EI + K + ++ V HVD GKST+ ++ + G I Sbjct: 155 KKQNPTDLVS--VPEIFEQSNPKPVV-HLVVTGHVDSGKSTMLGRIMFELGEI 204 >SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 940 Score = 25.0 bits (52), Expect = 6.3 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = -1 Query: 437 DPSVTRSAAVTSEEKSTCPGEAIKLIKKP 351 D + +AVTSE S EA+K KKP Sbjct: 516 DGTYATESAVTSEALSAARLEAVKASKKP 544 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 24.6 bits (51), Expect = 8.4 Identities = 10/19 (52%), Positives = 15/19 (78%) Frame = -1 Query: 314 NTKSFSSSSKNIEMAVDLM 258 + KSFSS+S NI+ +DL+ Sbjct: 92 SAKSFSSTSSNIDSNLDLL 110 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,060,304 Number of Sequences: 5004 Number of extensions: 40252 Number of successful extensions: 145 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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