BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0277 (769 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 126 3e-30 SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 34 0.019 SPBC16E9.19 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 29 0.73 SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 27 3.0 SPAC977.03 |||methyltransferase |Schizosaccharomyces pombe|chr 1... 26 5.2 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 26 6.8 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 26 6.8 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 6.8 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 9.0 >SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 590 Score = 126 bits (304), Expect = 3e-30 Identities = 73/168 (43%), Positives = 103/168 (61%), Gaps = 27/168 (16%) Frame = +3 Query: 255 LLPAIVELAEDTKWRVRLAIIEHMPLLAGQLGQEFFDEKLTSLCMSWLVDHVYAIREAAT 434 LLPAIV LAED +WRVRLAII+++PLLA QLG EFF+EK+ +LCMSWL DHVY+IREAA Sbjct: 402 LLPAIVTLAEDKQWRVRLAIIDYIPLLAQQLGVEFFNEKMGNLCMSWLEDHVYSIREAAI 461 Query: 435 LNLKKLVEQYGPQW---VLVLH*RAVR--------------------RCGKDITTRVLLP 545 NL+KL E +G +W ++ A+R ++ + +LP Sbjct: 462 KNLRKLTEIFGLEWATETIIPKFLAMRSHPNYLYRMTTIFAISEIAPALNAEVIEKQILP 521 Query: 546 TVLSMADDNVANVRFNVAKTLQIMAKYL----DPAVIQPQVKPVLEKL 677 T+ + +D + N+RFNVAK +++ L D V + Q+ P+LE+L Sbjct: 522 TLEQLVNDPIPNIRFNVAKAFEVLKPVLAAGGDSTVYEQQIIPLLEQL 569 Score = 89.4 bits (212), Expect = 5e-19 Identities = 43/82 (52%), Positives = 61/82 (74%) Frame = +1 Query: 1 EHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECP 180 + I++ I+P I++L+ D QHV++AL I L+P +G++ T E+LLP+FL LKDE P Sbjct: 317 KRIVLEEIIPVIQELINDPAQHVRAALGMNIGALAPQLGKEKTTEYLLPMFLELLKDENP 376 Query: 181 EVRLNIISNLECVNEVIGIQQL 246 EVRLNIIS LE VN+V+GI+ L Sbjct: 377 EVRLNIISKLEVVNKVVGIELL 398 Score = 39.9 bits (89), Expect = 4e-04 Identities = 34/141 (24%), Positives = 57/141 (40%) Frame = +3 Query: 255 LLPAIVELAEDTKWRVRLAIIEHMPLLAGQLGQEFFDEKLTSLCMSWLVDHVYAIREAAT 434 L+ V L +DT+ VR AI +P L + E++ + + D +R A Sbjct: 285 LIKPFVLLMKDTEQEVRRAIATQIPGFCELLDKRIVLEEIIPVIQELINDPAQHVRAALG 344 Query: 435 LNLKKLVEQYGPQWVLVLH*RAVRRCGKDITTRVLLPTVLSMADDNVANVRFNVAKTLQI 614 +N+ L Q G K+ TT LLP L + D VR N+ L++ Sbjct: 345 MNIGALAPQLG----------------KEKTTEYLLPMFLELLKDENPEVRLNIISKLEV 388 Query: 615 MAKYLDPAVIQPQVKPVLEKL 677 + K + ++ + P + L Sbjct: 389 VNKVVGIELLSQSLLPAIVTL 409 Score = 37.1 bits (82), Expect = 0.003 Identities = 20/73 (27%), Positives = 40/73 (54%) Frame = +1 Query: 22 ILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNII 201 +LP +L+ D N V+ + S + ++ +VG + + LLP +T +D+ VRL II Sbjct: 363 LLPMFLELLKDENPEVRLNIISKLEVVNKVVGIELLSQSLLPAIVTLAEDKQWRVRLAII 422 Query: 202 SNLECVNEVIGIQ 240 + + + +G++ Sbjct: 423 DYIPLLAQQLGVE 435 Score = 36.3 bits (80), Expect = 0.005 Identities = 36/141 (25%), Positives = 63/141 (44%) Frame = +3 Query: 207 PRVCE*SDRHPTAGAVLLPAIVELAEDTKWRVRLAIIEHMPLLAGQLGQEFFDEKLTSLC 386 P CE D+ ++ P I EL D VR A+ ++ LA QLG+E E L + Sbjct: 309 PGFCELLDKRIVLEEII-PVIQELINDPAQHVRAALGMNIGALAPQLGKEKTTEYLLPMF 367 Query: 387 MSWLVDHVYAIREAATLNLKKLVEQYGPQWVLVLH*RAVRRCGKDITTRVLLPTVLSMAD 566 + L D +R LN+ +E + G ++ ++ LLP ++++A+ Sbjct: 368 LELLKDENPEVR----LNIISKLEVVN------------KVVGIELLSQSLLPAIVTLAE 411 Query: 567 DNVANVRFNVAKTLQIMAKYL 629 D VR + + ++A+ L Sbjct: 412 DKQWRVRLAIIDYIPLLAQQL 432 Score = 31.9 bits (69), Expect = 0.10 Identities = 20/74 (27%), Positives = 35/74 (47%) Frame = +3 Query: 255 LLPAIVELAEDTKWRVRLAIIEHMPLLAGQLGQEFFDEKLTSLCMSWLVDHVYAIREAAT 434 LLP +EL +D VRL II + ++ +G E + L ++ D + +R A Sbjct: 363 LLPMFLELLKDENPEVRLNIISKLEVVNKVVGIELLSQSLLPAIVTLAEDKQWRVRLAII 422 Query: 435 LNLKKLVEQYGPQW 476 + L +Q G ++ Sbjct: 423 DYIPLLAQQLGVEF 436 Score = 27.1 bits (57), Expect = 3.0 Identities = 15/63 (23%), Positives = 32/63 (50%) Frame = +1 Query: 22 ILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNII 201 +L ++ V D++ + +A+ + L+ +VG + L+ F+ +KD EVR I Sbjct: 246 LLQPLRSFVSDSSWRTRYMVAANFVKLAKVVGPSLIKDELIKPFVLLMKDTEQEVRRAIA 305 Query: 202 SNL 210 + + Sbjct: 306 TQI 308 >SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizosaccharomyces pombe|chr 2|||Manual Length = 1706 Score = 34.3 bits (75), Expect = 0.019 Identities = 21/77 (27%), Positives = 40/77 (51%) Frame = +1 Query: 19 MILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNI 198 +IL + + D + ++ A I GLS +G ++ E++LPL L L D P V ++ Sbjct: 631 LILSHLITYLNDTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESV 690 Query: 199 ISNLECVNEVIGIQQLV 249 + + + E+ ++LV Sbjct: 691 LGSFSGLIELHLFEKLV 707 Score = 33.1 bits (72), Expect = 0.045 Identities = 19/69 (27%), Positives = 33/69 (47%) Frame = +3 Query: 252 VLLPAIVELAEDTKWRVRLAIIEHMPLLAGQLGQEFFDEKLTSLCMSWLVDHVYAIREAA 431 ++L ++ DT W +R A E + L+ +G DE + L + LVD A+ E+ Sbjct: 631 LILSHLITYLNDTDWMLRCAFFESITGLSIFIGPRSVDEYILPLMLQALVDPEPAVLESV 690 Query: 432 TLNLKKLVE 458 + L+E Sbjct: 691 LGSFSGLIE 699 Score = 26.6 bits (56), Expect = 3.9 Identities = 18/75 (24%), Positives = 40/75 (53%), Gaps = 2/75 (2%) Frame = +1 Query: 19 MILPQIKDLVCDAN-QHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLN 195 ++LP + + N + K S++ LS + ++ ++ +LP +T L+D+ +VR++ Sbjct: 431 VLLPIVLSTIRHVNTRESKINALSLVQILSRNICDESKLDTVLPFVMTLLRDQYADVRIS 490 Query: 196 -IISNLECVNEVIGI 237 +I+ V+ V I Sbjct: 491 ALITITRLVSNVTSI 505 >SPBC16E9.19 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 101 Score = 29.1 bits (62), Expect = 0.73 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = -3 Query: 323 VLDDGQTDPPLGVLCQLHDGGEEDCTSCWMPITS 222 + D T P +GV L GGE+D S W IT+ Sbjct: 14 ITGDMSTLPEIGVRTLLGGGGEDDIKSHWAQITA 47 >SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Schizosaccharomyces pombe|chr 1|||Manual Length = 1188 Score = 27.1 bits (57), Expect = 3.0 Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 3/75 (4%) Frame = +1 Query: 22 ILPQIKDLVCDANQHVKSALASVIMGLS---PIVGRQNTIEHLLPLFLTQLKDECPEVRL 192 I P + D + D + + ASVI LS +G ++ + HLL + + +E P V Sbjct: 1053 ITPLLADALMDRDAVHRQTAASVIKHLSLGCVGLGVEDAMIHLLNILWPNILEESPHVIN 1112 Query: 193 NIISNLECVNEVIGI 237 + ++ + IG+ Sbjct: 1113 AVREGIDGIRNCIGV 1127 >SPAC977.03 |||methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 145 Score = 26.2 bits (55), Expect = 5.2 Identities = 11/38 (28%), Positives = 17/38 (44%) Frame = -2 Query: 258 GGLHQLLDADHFIHTLEVGDYVESDFGTFILQLCEEQR 145 G H +L++DH HT + SD F+ E + Sbjct: 82 GCFHSILNSDHEPHTASLSHICHSDSSVFLRAFSETNK 119 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 25.8 bits (54), Expect = 6.8 Identities = 9/42 (21%), Positives = 19/42 (45%) Frame = -3 Query: 143 RRCSIVFCLPTMGLRPMMTEASADLTCWLASHTKSLICGKIM 18 R S+++CLP+ +P+ + +H + C +M Sbjct: 387 REASLLYCLPSTDFQPLFVSEKLSIQEVTYAHCVRIFCEHVM 428 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 25.8 bits (54), Expect = 6.8 Identities = 14/34 (41%), Positives = 14/34 (41%) Frame = +2 Query: 254 PPPRHRGAGRGHQVEGPSGHHRAHASTGGTAGPG 355 PPP G G G HH H GG GPG Sbjct: 200 PPPGPGGFGGFGGFGGEGHHHGGHGGFGG--GPG 231 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.8 bits (54), Expect = 6.8 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = +3 Query: 642 IQPQVKPVLEKLNVGPRRRRQVLR 713 ++ QV+ +L +LNVGPR Q L+ Sbjct: 562 LKEQVESMLSQLNVGPRDPMQFLK 585 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 25.4 bits (53), Expect = 9.0 Identities = 15/39 (38%), Positives = 24/39 (61%), Gaps = 1/39 (2%) Frame = -1 Query: 631 SKYLAIICSVL-ATLNRTLATLSSAIDNTVGSSTLVVMS 518 S Y A + ++ ++L+ T + SSAI +V SSTL+ S Sbjct: 707 STYSASLSNITHSSLSLTAMSSSSAIPTSVNSSTLITAS 745 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,152,218 Number of Sequences: 5004 Number of extensions: 65171 Number of successful extensions: 226 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 204 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 226 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 369323696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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