Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0255
(699 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g71100.1 68414.m08205 ribose 5-phosphate isomerase-related si... 64 9e-11
At2g01290.1 68415.m00043 expressed protein 63 2e-10
At3g04790.1 68416.m00516 ribose 5-phosphate isomerase-related si... 61 6e-10
At5g15690.1 68418.m01835 hypothetical protein very low similarit... 31 0.97
At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family... 31 0.97
At1g50900.1 68414.m05723 expressed protein 30 1.3
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 29 3.9
At3g04050.1 68416.m00427 pyruvate kinase, putative similar to py... 29 3.9
At5g63550.1 68418.m07976 expressed protein 28 5.2
At1g26110.1 68414.m03186 expressed protein 28 5.2
>At1g71100.1 68414.m08205 ribose 5-phosphate isomerase-related
similar to ribose-5-phosphate isomerase GI:18654317 from
[Spinacia oleracea]
Length = 267
Score = 64.1 bits (149), Expect = 9e-11
Identities = 32/96 (33%), Positives = 60/96 (62%), Gaps = 3/96 (3%)
Frame = +2
Query: 206 TSEASRGVSSCRSICY--DNCIFGVGSGSTVVYAVQRLAERVESEKLK-VTCIPTSFQAK 376
T E + +++ +++ + + G+G+GST +AV R++E + KLK + IPTS
Sbjct: 28 TQEELKKIAAYKAVEFVESGMVIGLGTGSTAKHAVARISELLREGKLKDIIGIPTSTTTH 87
Query: 377 QLIIKHGLNLGELETNPNIDVTIDGADEVDSNMTLI 484
+ + G+ L +L+++P +D++IDGADEVD + L+
Sbjct: 88 EQAVSLGIPLSDLDSHPVVDLSIDGADEVDPALNLV 123
Score = 33.1 bits (72), Expect = 0.18
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +1
Query: 490 RGRVSVKEKIIASCSKKLIVIADYTKDSVKLGDRYKKGVPIEVIP 624
RG ++EK+I SKK +VI D +K VK VP+EV+P
Sbjct: 126 RGGSLLREKMIEGASKKFVVIVDESK-LVKYIGGSGLAVPVEVVP 169
>At2g01290.1 68415.m00043 expressed protein
Length = 265
Score = 62.9 bits (146), Expect = 2e-10
Identities = 30/75 (40%), Positives = 50/75 (66%), Gaps = 1/75 (1%)
Frame = +2
Query: 263 IFGVGSGSTVVYAVQRLAERVESEKLK-VTCIPTSFQAKQLIIKHGLNLGELETNPNIDV 439
+ G+G+GST +AV R+ E + KL+ + IPTS + ++ + G+ L +L+ +P ID+
Sbjct: 53 VLGLGTGSTAKHAVDRIGELLRQGKLENIVGIPTSKKTQEQALSLGIPLSDLDAHPVIDL 112
Query: 440 TIDGADEVDSNMTLI 484
+IDGADEVD + L+
Sbjct: 113 SIDGADEVDPFLNLV 127
Score = 35.1 bits (77), Expect = 0.045
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = +1
Query: 490 RGRVSVKEKIIASCSKKLIVIADYTKDSVKLGDRYKKGVPIEVIPMAY 633
RG ++EK+I SKK +VI D +K +G K +P+E++P +
Sbjct: 130 RGGSLLREKMIEGASKKFVVIVDDSKMVKHIGGS-KLALPVEIVPFCW 176
>At3g04790.1 68416.m00516 ribose 5-phosphate isomerase-related
similar to ribose-5-phosphate isomerase GI:18654317 from
[Spinacia oleracea]
Length = 276
Score = 61.3 bits (142), Expect = 6e-10
Identities = 30/75 (40%), Positives = 49/75 (65%), Gaps = 1/75 (1%)
Frame = +2
Query: 263 IFGVGSGSTVVYAVQRLAERVESEKL-KVTCIPTSFQAKQLIIKHGLNLGELETNPNIDV 439
+ G+G+GST +AV ++ + + S +L + IPTS + ++ G+ L L+T+P ID+
Sbjct: 65 VLGLGTGSTAAFAVDQIGKLLSSGELYDIVGIPTSKRTEEQARSLGIPLVGLDTHPRIDL 124
Query: 440 TIDGADEVDSNMTLI 484
IDGADEVD N+ L+
Sbjct: 125 AIDGADEVDPNLDLV 139
Score = 31.5 bits (68), Expect = 0.56
Identities = 17/53 (32%), Positives = 29/53 (54%)
Frame = +1
Query: 475 DTYQRRGRVSVKEKIIASCSKKLIVIADYTKDSVKLGDRYKKGVPIEVIPMAY 633
D + RG ++EK++ + + K IV+AD TK LG +P+EV+ +
Sbjct: 137 DLVKGRGGALLREKMVEAVADKFIVVADDTKLVTGLGGS-GLAMPVEVVQFCW 188
>At5g15690.1 68418.m01835 hypothetical protein very low similarity
to MtN20 [Medicago truncatula] GI:2598591
Length = 169
Score = 30.7 bits (66), Expect = 0.97
Identities = 14/45 (31%), Positives = 21/45 (46%)
Frame = +2
Query: 158 KSSFLHTKYDVEDVFRTSEASRGVSSCRSICYDNCIFGVGSGSTV 292
+ L K +VE++ RT CR C D CI+ + G +V
Sbjct: 118 EGELLTMKENVEELGRTCHGKVSFEDCRGCCCDCCIYCMALGKSV 162
>At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family
protein
Length = 635
Score = 30.7 bits (66), Expect = 0.97
Identities = 28/78 (35%), Positives = 36/78 (46%), Gaps = 4/78 (5%)
Frame = -1
Query: 489 PLISVMFESTSSAPSIVTSIFGFVSSSPRLSPCLIISCFA*KEVGMQVTFSFSDST-RSA 313
PL S S SAP+ +TS+ + SP SP L I FA + S + S S+
Sbjct: 139 PLHSSSPFSFGSAPAAITSVSSGPAQSPASSPRLWIDRFATSSSASATSSSSTSSPFHSS 198
Query: 312 SL---CTA*TTVDPLPTP 268
SL A T+V PTP
Sbjct: 199 SLLGFAPAVTSVSSAPTP 216
>At1g50900.1 68414.m05723 expressed protein
Length = 175
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = -1
Query: 465 STSSAPSIVTSIFGFVSSSPRL 400
S S APS+ TS+F SSSPRL
Sbjct: 7 SFSCAPSLATSLFSTTSSSPRL 28
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 28.7 bits (61), Expect = 3.9
Identities = 17/54 (31%), Positives = 27/54 (50%)
Frame = +2
Query: 281 GSTVVYAVQRLAERVESEKLKVTCIPTSFQAKQLIIKHGLNLGELETNPNIDVT 442
GST + AVQ L +V ++ VT P A ++ G+ G++ +DVT
Sbjct: 413 GSTRIPAVQELVRKVTGKEPNVTVNPDEVVALGAAVQAGVLAGDVSDIVLLDVT 466
>At3g04050.1 68416.m00427 pyruvate kinase, putative similar to
pyruvate kinase, cytosolic isozyme [Nicotiana tabacum]
SWISS-PROT:Q42954
Length = 510
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +1
Query: 520 IASCSKKLIVIADYTKDSVKLGDRYKKGVPIEVIPMAYVPIKKQNHIIVW 669
+ SC K L ++ ++S LG+R +P V+ + + K Q I+ W
Sbjct: 148 VLSCDKNLGLVRARCENSAVLGERKNVNLPGIVVDLPTLTEKDQEDILQW 197
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 28.3 bits (60), Expect = 5.2
Identities = 18/69 (26%), Positives = 33/69 (47%)
Frame = +1
Query: 484 QRRGRVSVKEKIIASCSKKLIVIADYTKDSVKLGDRYKKGVPIEVIPMAYVPIKKQNHII 663
+ + R +KEKI +KLIV D + + K+ + ++V+ P K+ +I
Sbjct: 188 EEKQRARIKEKIDKCVKEKLIVFCDVLDIPISRSNVKKEELAVKVLEFLESP-KETRDVI 246
Query: 664 VWR*NQAKK 690
+ +AKK
Sbjct: 247 IADQEKAKK 255
>At1g26110.1 68414.m03186 expressed protein
Length = 611
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/35 (42%), Positives = 20/35 (57%)
Frame = -1
Query: 498 PPPPLISVMFESTSSAPSIVTSIFGFVSSSPRLSP 394
PPPP S + SS P +S+F F +SS L+P
Sbjct: 217 PPPPTGSSSLQG-SSLPEAPSSLFPFSTSSQMLAP 250
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,848,073
Number of Sequences: 28952
Number of extensions: 243013
Number of successful extensions: 639
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 621
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 636
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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