Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0250
(533 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 52 3e-07
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 50 1e-06
At1g29050.1 68414.m03556 expressed protein similar to hypothetic... 29 2.6
At2g24610.1 68415.m02940 cyclic nucleotide-regulated ion channel... 28 3.4
At1g52150.2 68414.m05885 homeobox-leucine zipper family protein ... 28 4.5
At1g52150.1 68414.m05884 homeobox-leucine zipper family protein ... 28 4.5
At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative / arg... 27 5.9
At4g20730.1 68417.m03013 filament protein-related similar to Cyt... 27 5.9
At3g10100.1 68416.m01210 filament protein-related similar to YEA... 27 5.9
At5g24240.1 68418.m02852 phosphatidylinositol 3- and 4-kinase fa... 27 7.9
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot... 27 7.9
At4g26090.1 68417.m03756 disease resistance protein RPS2 (CC-NBS... 27 7.9
At2g48050.1 68415.m06014 expressed protein ; expression supporte... 27 7.9
At2g09910.1 68415.m01029 hypothetical protein 27 7.9
At1g70060.1 68414.m08061 paired amphipathic helix repeat-contain... 27 7.9
>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 51.6 bits (118), Expect = 3e-07
Identities = 24/73 (32%), Positives = 42/73 (57%)
Frame = +2
Query: 281 ERGVTSLVTALNKGQLEGRLLEFLPLNRRS*EALGAAFSSRGLAELLRLHRAQASQEALR 460
E + L++ L +G++E L++FLP RRS E+ F++ GL +L+ H + + LR
Sbjct: 195 ENSLEDLISILRRGKMEDNLMDFLPPVRRSAESFAEHFTNEGLTDLVEYHSKKMFEVKLR 254
Query: 461 ELTASLLEQLAEE 499
E+ L ++ EE
Sbjct: 255 EIKTVLTSKVTEE 267
Score = 37.5 bits (83), Expect = 0.006
Identities = 24/74 (32%), Positives = 39/74 (52%), Gaps = 3/74 (4%)
Frame = +3
Query: 42 KLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIG---NGCVPPSVLLVLV 212
K++RR +L K E ++ L L+ F+ +R KLA TAL +G P +V L+
Sbjct: 112 KILRRKPFLIKNLENVTRRFLQSLELFEENERKKLAIFTALAFSQKLSGLPPETVFQPLL 171
Query: 213 NEHLLKDNLALDFV 254
++L+ + L FV
Sbjct: 172 KDNLVAKGIVLSFV 185
>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein low similarity to SP|P47823
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Oryctolagus
cuniculus}; contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 411
Score = 49.6 bits (113), Expect = 1e-06
Identities = 23/73 (31%), Positives = 42/73 (57%)
Frame = +2
Query: 281 ERGVTSLVTALNKGQLEGRLLEFLPLNRRS*EALGAAFSSRGLAELLRLHRAQASQEALR 460
E + L++ L +G+++ +LLEFLP +R+ E+ F+ GL L+ + + + L+
Sbjct: 195 ENSLDDLISILRRGKMDDKLLEFLPPTKRTTESFAEHFTKAGLTALVEYNERKIFEVKLK 254
Query: 461 ELTASLLEQLAEE 499
E+ A L Q+ EE
Sbjct: 255 EIKAVLTSQVTEE 267
Score = 33.5 bits (73), Expect = 0.091
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 3/74 (4%)
Frame = +3
Query: 42 KLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIG---NGCVPPSVLLVLV 212
K++RR +L K E ++ L L+ F+ +R KLA TAL +G +V L+
Sbjct: 112 KILRRKAFLIKNLENVTRRFLQSLELFEENERKKLAIFTALAFSQKLSGLPAETVFQPLL 171
Query: 213 NEHLLKDNLALDFV 254
+ L+ + L+FV
Sbjct: 172 KDTLVAKGIVLNFV 185
>At1g29050.1 68414.m03556 expressed protein similar to hypothetical
protein GB:AAB67625 GI:2342727 from [Arabidopsis
thaliana]
Length = 380
Score = 28.7 bits (61), Expect = 2.6
Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 3/60 (5%)
Frame = -1
Query: 218 FVHQDQQHGWRHASVTDPQCSHARQLDALF-GIEAFQ--VDQNLFHFLFEHFFQVLVPTH 48
+ H+ Q GW + +LDA + G+ + VDQN+ FFQ + PTH
Sbjct: 224 WTHKGQSQGWDYIRDGSSLVRDMNRLDAFYKGLSTWARWVDQNVDTAKTRVFFQGISPTH 283
>At2g24610.1 68415.m02940 cyclic nucleotide-regulated ion channel,
putative (CNGC14) similar to cyclic nucleotide and
calmodulin-regulated ion channel (GI:4581205)
[Arabidopsis thaliana]
Length = 726
Score = 28.3 bits (60), Expect = 3.4
Identities = 10/40 (25%), Positives = 22/40 (55%)
Frame = -1
Query: 215 VHQDQQHGWRHASVTDPQCSHARQLDALFGIEAFQVDQNL 96
+H+D ++ W++ +V C + + FGI A + +N+
Sbjct: 302 LHRDDRNNWQNTTVVFSNCDPSNNIQFTFGIFADALTKNV 341
>At1g52150.2 68414.m05885 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
to HD-zip transcription factor (athb-8) (GI:7270235)
[Arabidopsis thaliana]; contains Pfam profiles PF01852:
START domain, PF00046: Homeobox domain
Length = 837
Score = 27.9 bits (59), Expect = 4.5
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +3
Query: 102 LVYLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEH 221
L + GF P + L ++ + N VPP++LL + EH
Sbjct: 440 LTFANGFAPVSNVVLCAKASMLLQN--VPPAILLRFLREH 477
>At1g52150.1 68414.m05884 homeobox-leucine zipper family protein /
lipid-binding START domain-containing protein similar to
to HD-zip transcription factor (athb-8) (GI:7270235)
[Arabidopsis thaliana]; contains Pfam profiles PF01852:
START domain, PF00046: Homeobox domain
Length = 836
Score = 27.9 bits (59), Expect = 4.5
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = +3
Query: 102 LVYLKGFDPEQRIKLARMTALWIGNGCVPPSVLLVLVNEH 221
L + GF P + L ++ + N VPP++LL + EH
Sbjct: 440 LTFANGFAPVSNVVLCAKASMLLQN--VPPAILLRFLREH 477
>At4g26300.1 68417.m03783 arginyl-tRNA synthetase, putative /
arginine--tRNA ligase, putative similar to SP|P37880
Arginyl-tRNA synthetase (EC 6.1.1.19) (Arginine--tRNA
ligase) (ArgRS) {Cricetulus longicaudatus}; contains
Pfam profiles PF00750: arginyl-tRNA synthetase, PF03485:
arginyl-tRNA synthetase N-terminal domain
Length = 642
Score = 27.5 bits (58), Expect = 5.9
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +3
Query: 30 QVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARM 155
QVF K + L++ F+E+ ++ +V L+G DP R A++
Sbjct: 260 QVFYKASKHKFDLDEAFKEKAQQAVVRLQGGDPVYRKAWAKI 301
>At4g20730.1 68417.m03013 filament protein-related similar to
Cytadherence high molecular weight protein 2 (SP:P47460)
[Mycoplasma genitalium]; similar to YEAST NUF1
protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; also
SP|Q9UKX2|MYH2_HUMAN Myosin heavy chain, skeletal
muscle, SP|P31732|OV71_ONCVO Muscle cell intermediate
filament protein SP|P12882|MYH1_HUMAN Myosin heavy
chain, skeletal muscle,. SP|Q17107|AV71_ACAVI Muscle
cell intermediate filament protein
Length = 800
Score = 27.5 bits (58), Expect = 5.9
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = +2
Query: 41 ETDASVQVLGKNVRRGNEKGSGLLERLRSRTAHQAG 148
+ D +V LG + R EKG G+ + + R+A +AG
Sbjct: 455 DKDETVAELGISRRSREEKGKGVATQSKKRSASEAG 490
>At3g10100.1 68416.m01210 filament protein-related similar to YEAST
NUF1 protein (Spindle poly body spacer protein SPC110)
(SP:P32380) {Saccharomyces cerevisiae}; similar to
Myosin heavy chain, smooth muscle isoform (SMMHC)
(SP:P35749) {Homo sapiens}
Length = 1004
Score = 27.5 bits (58), Expect = 5.9
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 56 VQVLGKNVRRGNEKGSGLLERLRSRTAHQAGAHDCTVDR 172
V LG + R EKG GL + + R+A +AG + +
Sbjct: 459 VAELGASRRSREEKGKGLASQSKKRSASEAGLDEAAAPK 497
>At5g24240.1 68418.m02852 phosphatidylinositol 3- and 4-kinase
family protein / ubiquitin family protein contains
INTERPRO:IPR000626 ubiquitin domain, Pfam profile
PF00454: Phosphatidylinositol 3- and 4-kinase
Length = 574
Score = 27.1 bits (57), Expect = 7.9
Identities = 23/74 (31%), Positives = 32/74 (43%), Gaps = 1/74 (1%)
Frame = +3
Query: 24 FEQVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIGNGCVPPSVLL 203
F F + Y + E + L Y+K DPE+ I+L R W +PPS
Sbjct: 445 FPNKFEDCTFEWLYWPQAKEPYSSETLEYIKSLDPEKDIELLRFHG-WE----IPPSCTR 499
Query: 204 VL-VNEHLLKDNLA 242
VL ++ LLK A
Sbjct: 500 VLRISTMLLKKGSA 513
>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor [Homo
sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1706
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +3
Query: 72 KMFEEEMKKVLVYLKGFDPEQRIKLARMTALWI 170
++ E K V +YLK DP+Q+ + R LW+
Sbjct: 1600 RLVTECAKIVKIYLKCTDPQQQEQQQRKPVLWV 1632
>At4g26090.1 68417.m03756 disease resistance protein RPS2
(CC-NBS-LRR class), putative domain signature CC-NBS-LRR
exists, suggestive of a disease resistance protein.
identical to RPS2 (gi:13661831)
Length = 909
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/60 (25%), Positives = 26/60 (43%)
Frame = +3
Query: 15 MRNFEQVFVKLMRRYKYLEKMFEEEMKKVLVYLKGFDPEQRIKLARMTALWIGNGCVPPS 194
M+ VF L Y LE + ++ +Y F E I++ ++ W+G G + S
Sbjct: 382 MKGMNYVFALLKFSYDNLES---DLLRSCFLYCALFPEEHSIEIEQLVEYWVGEGFLTSS 438
>At2g48050.1 68415.m06014 expressed protein ; expression supported by
MPSS
Length = 1500
Score = 27.1 bits (57), Expect = 7.9
Identities = 15/43 (34%), Positives = 21/43 (48%), Gaps = 1/43 (2%)
Frame = -3
Query: 300 SEVTPRSCLMIANTSTRSPAPGCPSAD-VRSPGPAARMEARIR 175
S TP L++ SP GC SA+ +S PA+ + IR
Sbjct: 1233 STETPNEALVVLEVEYASPTNGCSSAEWYKSLTPASDVAKEIR 1275
>At2g09910.1 68415.m01029 hypothetical protein
Length = 985
Score = 27.1 bits (57), Expect = 7.9
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = +2
Query: 38 RETDASVQVLGKNVRRGNEKGSGLLERLRSRTAHQAG 148
++ D +V+ L + R EKG G+ + + R+A +AG
Sbjct: 419 QDKDETVEELETSRRSREEKGKGVANQSKKRSASEAG 455
>At1g70060.1 68414.m08061 paired amphipathic helix repeat-containing
protein similar to transcription co-repressor Sin3
[Xenopus laevis] GI:4960210; contains Pfam profile
PF02671: Paired amphipathic helix repeat
Length = 1362
Score = 27.1 bits (57), Expect = 7.9
Identities = 11/44 (25%), Positives = 22/44 (50%)
Frame = -1
Query: 254 HEVQRQVVLQQMFVHQDQQHGWRHASVTDPQCSHARQLDALFGI 123
H++ +L W+ ++ T+P S+AR +DAL+ +
Sbjct: 1088 HQILYDRILSAKINSSSPDRKWKTSNPTNPADSYARIMDALYNL 1131
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,191,662
Number of Sequences: 28952
Number of extensions: 196216
Number of successful extensions: 778
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 777
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 984125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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