Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0247
(481 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 126 1e-29
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 95 3e-20
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 93 9e-20
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 93 9e-20
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 89 2e-18
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 59 2e-09
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 43 9e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 43 9e-05
At2g47500.1 68415.m05929 kinesin motor protein-related 30 0.93
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 28 3.7
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 27 5.0
At5g43300.1 68418.m05292 glycerophosphoryl diester phosphodieste... 27 8.7
At3g22160.1 68416.m02797 VQ motif-containing protein contains PF... 27 8.7
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 27 8.7
At1g37020.1 68414.m04616 Ulp1 protease family protein 27 8.7
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 126 bits (303), Expect = 1e-29
Identities = 68/100 (68%), Positives = 74/100 (74%), Gaps = 1/100 (1%)
Frame = +3
Query: 3 KFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQCINEESGEHIVAGAGELHLEIC 182
KFSVSPVVRVAV+ K +DLPKLVEGLKRLAKS PMV C EESGEHIVAGAGELHLEIC
Sbjct: 482 KFSVSPVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEIC 541
Query: 183 LKGLEEDH-ACIPIKKSDPVVSYREP*LRNRTSSVSQSRP 299
LK L++D I KSDPVVS+RE T +V P
Sbjct: 542 LKDLQDDFMGGAEIIKSDPVVSFRETVCDRSTRTVMSKSP 581
Score = 95.5 bits (227), Expect = 2e-20
Identities = 40/69 (57%), Positives = 54/69 (78%)
Frame = +2
Query: 269 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEGIDEGRVNPRDDFKTRARYLTEKYGYDVTEA 448
S + +SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K R++ L E++G+D A
Sbjct: 572 STRTVMSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLA 631
Query: 449 RKIWCFGPE 475
+KIW FGPE
Sbjct: 632 KKIWAFGPE 640
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 94.7 bits (225), Expect = 3e-20
Identities = 42/84 (50%), Positives = 62/84 (73%)
Frame = +3
Query: 3 KFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQCINEESGEHIVAGAGELHLEIC 182
KF+ PVV+ A EP NP++LPK+VEGL++++KSYP+ EESGEH + G GEL+L+
Sbjct: 581 KFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSI 640
Query: 183 LKGLEEDHACIPIKKSDPVVSYRE 254
+K L E ++ + +K +DPVVS+ E
Sbjct: 641 IKDLRELYSEVQVKVADPVVSFCE 664
Score = 55.6 bits (128), Expect = 2e-08
Identities = 26/69 (37%), Positives = 39/69 (56%)
Frame = +2
Query: 269 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEGIDEGRVNPRDDFKTRARYLTEKYGYDVTEA 448
S C +++PNK N+L M A+P+ GL E I+ G V+ + + KY +D+ A
Sbjct: 670 SSMKCFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAA 729
Query: 449 RKIWCFGPE 475
R IW FGP+
Sbjct: 730 RSIWAFGPD 738
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 93.1 bits (221), Expect = 9e-20
Identities = 41/84 (48%), Positives = 62/84 (73%)
Frame = +3
Query: 3 KFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQCINEESGEHIVAGAGELHLEIC 182
+F+ PVV+ A EP NP++LPK+VEGL++++KSYP+ EESGEH + G GEL+L+
Sbjct: 595 QFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSI 654
Query: 183 LKGLEEDHACIPIKKSDPVVSYRE 254
+K L E ++ + +K +DPVVS+ E
Sbjct: 655 MKDLRELYSEVEVKVADPVVSFCE 678
Score = 57.6 bits (133), Expect = 4e-09
Identities = 26/69 (37%), Positives = 40/69 (57%)
Frame = +2
Query: 269 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEGIDEGRVNPRDDFKTRARYLTEKYGYDVTEA 448
S C +++PNK N++ M A+P+ GL E I+ G V+ + K + KY +D+ A
Sbjct: 684 SSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAA 743
Query: 449 RKIWCFGPE 475
R IW FGP+
Sbjct: 744 RSIWAFGPD 752
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 93.1 bits (221), Expect = 9e-20
Identities = 41/84 (48%), Positives = 62/84 (73%)
Frame = +3
Query: 3 KFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQCINEESGEHIVAGAGELHLEIC 182
+F+ PVV+ A EP NP++LPK+VEGL++++KSYP+ EESGEH + G GEL+L+
Sbjct: 595 QFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSI 654
Query: 183 LKGLEEDHACIPIKKSDPVVSYRE 254
+K L E ++ + +K +DPVVS+ E
Sbjct: 655 MKDLRELYSEVEVKVADPVVSFCE 678
Score = 57.6 bits (133), Expect = 4e-09
Identities = 26/69 (37%), Positives = 40/69 (57%)
Frame = +2
Query: 269 SDQLCLSKSPNKHNRLFMKAQPMPDGLPEGIDEGRVNPRDDFKTRARYLTEKYGYDVTEA 448
S C +++PNK N++ M A+P+ GL E I+ G V+ + K + KY +D+ A
Sbjct: 684 SSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAA 743
Query: 449 RKIWCFGPE 475
R IW FGP+
Sbjct: 744 RSIWAFGPD 752
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 88.6 bits (210), Expect = 2e-18
Identities = 40/84 (47%), Positives = 60/84 (71%)
Frame = +3
Query: 3 KFSVSPVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQCINEESGEHIVAGAGELHLEIC 182
+F VSP +RVA+EP +PAD+ L++GL+ L ++ P V+ GEH++A AGE+HLE C
Sbjct: 532 EFQVSPTLRVAIEPSDPADMSALMKGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERC 591
Query: 183 LKGLEEDHACIPIKKSDPVVSYRE 254
+K L+E A + ++ S P+VSYRE
Sbjct: 592 VKDLKERFAKVNLEVSPPLVSYRE 615
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 58.8 bits (136), Expect = 2e-09
Identities = 31/80 (38%), Positives = 47/80 (58%), Gaps = 1/80 (1%)
Frame = +3
Query: 18 PVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQ-CINEESGEHIVAGAGELHLEICLKGL 194
PV++VA+EPK AD+ K+ GL +LA+ P +EE + ++ G GELHLEI + L
Sbjct: 493 PVIKVAIEPKTKADIDKMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRL 552
Query: 195 EEDHACIPIKKSDPVVSYRE 254
+ + + P V+YRE
Sbjct: 553 KREFK-VEANVGAPQVNYRE 571
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 43.2 bits (97), Expect = 9e-05
Identities = 24/80 (30%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
Frame = +3
Query: 18 PVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQC-INEESGEHIVAGAGELHLEICLKGL 194
PV+ +AV+P + + + L R K P + ++ ESG+ I++G GELHL+I ++ +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 195 EEDHACIPIKKSDPVVSYRE 254
++ + P V++RE
Sbjct: 521 RREYK-VDATVGKPRVNFRE 539
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 43.2 bits (97), Expect = 9e-05
Identities = 24/80 (30%), Positives = 45/80 (56%), Gaps = 1/80 (1%)
Frame = +3
Query: 18 PVVRVAVEPKNPADLPKLVEGLKRLAKSYPMVQC-INEESGEHIVAGAGELHLEICLKGL 194
PV+ +AV+P + + + L R K P + ++ ESG+ I++G GELHL+I ++ +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 195 EEDHACIPIKKSDPVVSYRE 254
++ + P V++RE
Sbjct: 521 RREYK-VDATVGKPRVNFRE 539
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 29.9 bits (64), Expect = 0.93
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 9 SVSPVVRVAVEPKNPADLPKLVEGL 83
S+S +VR + K P D+PKL+E L
Sbjct: 229 SLSTLVRAVLSDKKPEDVPKLIESL 253
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 27.9 bits (59), Expect = 3.7
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 5/56 (8%)
Frame = +2
Query: 287 SKSPNKHNRLFMKAQPM-----PDGLPEGIDEGRVNPRDDFKTRARYLTEKYGYDV 439
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 27.5 bits (58), Expect = 5.0
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2
Query: 272 DQLCLSKSPNKHNRLFMKAQPMPDGLPEGI 361
+ LCL PN+H + + A+ +P LPE +
Sbjct: 46 ENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At5g43300.1 68418.m05292 glycerophosphoryl diester
phosphodiesterase family protein contains Pfam profile
PF03009: Glycerophosphoryl diester phosphodiesterase
family
Length = 333
Score = 26.6 bits (56), Expect = 8.7
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -1
Query: 319 EQTVVLVGRL*ETELVRFLSYGSRYDTT 236
EQ V++ R+ E +L FLSYG + D T
Sbjct: 64 EQGVIIEKRVTEMDLHEFLSYGPQRDGT 91
>At3g22160.1 68416.m02797 VQ motif-containing protein contains
PF05678: VQ motif
Length = 192
Score = 26.6 bits (56), Expect = 8.7
Identities = 13/40 (32%), Positives = 21/40 (52%)
Frame = -1
Query: 244 DTTGSDFLIGMQAWSSSRPLRQISRWSSPAPATMCSPDSS 125
DTT D + + ++P R+ SR S P T+ + D+S
Sbjct: 35 DTTSIDSRLSPETGRVTKPTRRRSRASRRTPTTLLNTDTS 74
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 26.6 bits (56), Expect = 8.7
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +3
Query: 120 INEESGEHIVAGAGELHLEICLKGLEEDH 206
I EE G G G+ ICL+ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At1g37020.1 68414.m04616 Ulp1 protease family protein
Length = 611
Score = 26.6 bits (56), Expect = 8.7
Identities = 12/39 (30%), Positives = 22/39 (56%), Gaps = 1/39 (2%)
Frame = -3
Query: 269 IPQLRLTVRHDRVR-LLDWNASMVLLKTLKTDLKMEFSS 156
IP+ V +++ L+DW M+L++ +K D +SS
Sbjct: 386 IPEHLQPVSDEKLHSLMDWLGLMILIRKMKNDYNKHYSS 424
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,754,574
Number of Sequences: 28952
Number of extensions: 217269
Number of successful extensions: 628
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 614
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 625
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 819227264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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