Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0244
(762 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2; Endopter... 182 9e-45
UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep: CG3... 102 8e-21
UniRef50_P43307 Cluster: Translocon-associated protein subunit a... 90 5e-17
UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella ve... 87 4e-16
UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2; ... 76 1e-12
UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;... 73 8e-12
UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole... 66 7e-10
UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1; ... 52 2e-05
UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2; Schis... 51 3e-05
UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole... 38 0.21
UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Re... 38 0.27
UniRef50_P45434 Cluster: Translocon-associated protein subunit a... 36 0.83
UniRef50_Q54VL8 Cluster: Putative uncharacterized protein; n=1; ... 35 1.9
UniRef50_Q24U67 Cluster: Putative uncharacterized protein; n=2; ... 33 5.8
>UniRef50_Q2F5L0 Cluster: Signal sequence receptor; n=2;
Endopterygota|Rep: Signal sequence receptor - Bombyx
mori (Silk moth)
Length = 279
Score = 182 bits (443), Expect = 9e-45
Identities = 85/85 (100%), Positives = 85/85 (100%)
Frame = +1
Query: 256 VVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP 435
VVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP
Sbjct: 58 VVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP 117
Query: 436 MDYTYYIQNFTALPYNREVKPKQEA 510
MDYTYYIQNFTALPYNREVKPKQEA
Sbjct: 118 MDYTYYIQNFTALPYNREVKPKQEA 142
Score = 133 bits (321), Expect = 5e-30
Identities = 62/65 (95%), Positives = 63/65 (96%)
Frame = +3
Query: 498 KTGSTFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF 677
K +TFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF
Sbjct: 139 KQEATFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF 198
Query: 678 FLYVF 692
FLYVF
Sbjct: 199 FLYVF 203
Score = 81.0 bits (191), Expect = 3e-14
Identities = 41/59 (69%), Positives = 43/59 (72%)
Frame = +2
Query: 86 MKLIYFTFLIVPAALLCLGSGNILARAXXXXXXXXXXXXXXXNQVVGDDALVDDDEMLL 262
MKLIYFTFLIVPAALLCLGSGNILARA NQVVGDDALVDDDE ++
Sbjct: 1 MKLIYFTFLIVPAALLCLGSGNILARAEEDELDDVVDIEGEDNQVVGDDALVDDDESVV 59
>UniRef50_Q9W335 Cluster: CG32701-PA; n=5; Endopterygota|Rep:
CG32701-PA - Drosophila melanogaster (Fruit fly)
Length = 302
Score = 102 bits (245), Expect = 8e-21
Identities = 46/82 (56%), Positives = 63/82 (76%)
Frame = +1
Query: 265 SSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDY 444
SS + DT +LFTKP+ + G D+ G PVEF +GF NKG+ ++V+E++EASFRYPMD+
Sbjct: 64 SSPNADTYLLFTKPLYTPGQ-QLDLPGGKPVEFLIGFTNKGAEEFVIETVEASFRYPMDF 122
Query: 445 TYYIQNFTALPYNREVKPKQEA 510
Y+IQNF+A+ YNREVKP E+
Sbjct: 123 NYFIQNFSAVAYNREVKPGFES 144
Score = 93.5 bits (222), Expect = 5e-18
Identities = 41/61 (67%), Positives = 51/61 (83%)
Frame = +3
Query: 507 STFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFFLY 686
ST +Y+F+P++ FAGRPFGLN+ L YRDA+G Y EAV+N+T+ I EV EGLDGETFFLY
Sbjct: 144 STVSYTFLPSDQFAGRPFGLNIALAYRDANGIQYNEAVFNETVLISEVDEGLDGETFFLY 203
Query: 687 V 689
V
Sbjct: 204 V 204
>UniRef50_P43307 Cluster: Translocon-associated protein subunit
alpha precursor; n=51; Euteleostomi|Rep:
Translocon-associated protein subunit alpha precursor -
Homo sapiens (Human)
Length = 286
Score = 90.2 bits (214), Expect = 5e-17
Identities = 36/62 (58%), Positives = 49/62 (79%)
Frame = +3
Query: 507 STFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFFLY 686
+TF YSFIP E GRPFGL + LNY+D +GN +Q+AV+NQT+ ++E +GLDGET F+Y
Sbjct: 152 ATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMY 211
Query: 687 VF 692
+F
Sbjct: 212 MF 213
Score = 89.0 bits (211), Expect = 1e-16
Identities = 48/95 (50%), Positives = 62/95 (65%), Gaps = 3/95 (3%)
Frame = +1
Query: 262 KSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMD 441
++S DTTILF K G+ D A V+F VGF NKG+ D++VES++ASFRYP D
Sbjct: 78 EASPSADTTILFVK-----GE---DFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQD 129
Query: 442 YTYYIQNFTALPYNREVKPKQEAPLP---IPSSPM 537
Y +YIQNFTALP N V P+++A IP+ PM
Sbjct: 130 YQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPM 164
>UniRef50_A7RRI8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 291
Score = 87.0 bits (206), Expect = 4e-16
Identities = 34/62 (54%), Positives = 50/62 (80%)
Frame = +3
Query: 507 STFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFFLY 686
++F Y+F P+E+F GRPFGL++ L Y+D G Y++AV+N+T+ +VE EG+DGETFFLY
Sbjct: 157 ASFEYAFRPHESFGGRPFGLSINLLYKDTDGKMYRDAVFNETVQLVESDEGMDGETFFLY 216
Query: 687 VF 692
+F
Sbjct: 217 LF 218
Score = 80.2 bits (189), Expect = 5e-14
Identities = 42/86 (48%), Positives = 49/86 (56%)
Frame = +1
Query: 253 DVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRY 432
D +K S D DT ILFTK AG + V F N G D++V+ MEAS RY
Sbjct: 78 DALKPSPDADTVILFTKNTEQ------QFSAGLVSKCLVSFSNNGRNDFMVDMMEASLRY 131
Query: 433 PMDYTYYIQNFTALPYNREVKPKQEA 510
P DY+YYIQNFTA YN VKP +A
Sbjct: 132 PQDYSYYIQNFTAFQYNTVVKPGHQA 157
>UniRef50_Q95XT5 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 257
Score = 75.8 bits (178), Expect = 1e-12
Identities = 34/79 (43%), Positives = 49/79 (62%)
Frame = +1
Query: 280 DTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQ 459
D + F PS ++ + G PV++ +GF NKG D+VV+ E SFR+P D++Y++Q
Sbjct: 48 DAGLAFHFVQPSDANVVREFYTGKPVKYLIGFQNKGEKDFVVKYAETSFRFPTDHSYHLQ 107
Query: 460 NFTALPYNREVKPKQEAPL 516
NFT YNR V PK+E L
Sbjct: 108 NFTRGEYNRRVAPKEEVTL 126
Score = 74.5 bits (175), Expect = 3e-12
Identities = 38/90 (42%), Positives = 50/90 (55%)
Frame = +3
Query: 423 FPVSNGLHILHSELHRVAL*QRS*AKTGSTFAYSFIPNEAFAGRPFGLNVQLNYRDASGN 602
FP + H+ R +R K T Y F +E FAGRP GL V ++Y+DA GN
Sbjct: 98 FPTDHSYHL--QNFTRGEYNRRVAPKEEVTLDYGFYAHETFAGRPVGLVVNVHYQDADGN 155
Query: 603 AYQEAVYNQTLNIVEVSEGLDGETFFLYVF 692
+ VYNQT+NI+E G GET FL++F
Sbjct: 156 VFVNNVYNQTINIMEDDSGFSGETGFLFIF 185
>UniRef50_UPI0000E46DC1 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 247
Score = 72.9 bits (171), Expect = 8e-12
Identities = 36/84 (42%), Positives = 52/84 (61%)
Frame = +1
Query: 259 VKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYPM 438
+K+S D DTT LFT + G+ +I P++ VGF N G D+ +ES+ A+ RYP
Sbjct: 88 LKASPDADTTFLFT----TNGEK--EIPVTIPLQVLVGFTNNGQQDFTIESLHATLRYPQ 141
Query: 439 DYTYYIQNFTALPYNREVKPKQEA 510
D+++YI NFT YN V+P +EA
Sbjct: 142 DFSFYIYNFTEKEYNTVVEPNKEA 165
>UniRef50_Q4T9K6 Cluster: Chromosome undetermined SCAF7543, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF7543,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 316
Score = 66.5 bits (155), Expect = 7e-10
Identities = 34/86 (39%), Positives = 47/86 (54%), Gaps = 1/86 (1%)
Frame = +3
Query: 438 GLHILHSELHRVAL*QRS*AKTGSTFAYSFIPNEAFAGRP-FGLNVQLNYRDASGNAYQE 614
GL +LH ELH + P A G L + LNY+D++GN +Q+
Sbjct: 159 GLPVLHPELHCSPAGNCGPSHATGHLRVLLHPRRAHGGGGRSALVINLNYKDSAGNVFQD 218
Query: 615 AVYNQTLNIVEVSEGLDGETFFLYVF 692
AV+NQT+ + E +GLDGET F+YVF
Sbjct: 219 AVFNQTVTVTEKEDGLDGETIFMYVF 244
Score = 54.0 bits (124), Expect = 4e-06
Identities = 31/59 (52%), Positives = 39/59 (66%)
Frame = +1
Query: 259 VKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRYP 435
VK+S DTTILF K G+ D A V+F +GF NKGS ++VVES++ASFRYP
Sbjct: 84 VKASPHADTTILFVK-----GE---DFPANNIVKFLLGFTNKGSENFVVESLDASFRYP 134
>UniRef50_Q54R45 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 236
Score = 51.6 bits (118), Expect = 2e-05
Identities = 23/59 (38%), Positives = 35/59 (59%)
Frame = +3
Query: 507 STFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFFL 683
+T AY F+P+E R FGL + L Y+D + N + + +N T+NI E D ++FFL
Sbjct: 110 ATLAYRFVPSEYLEPREFGLLISLEYQDGAQN-FTQTFFNSTINITEKETSFDMDSFFL 167
Score = 37.9 bits (84), Expect = 0.27
Identities = 28/98 (28%), Positives = 44/98 (44%)
Frame = +1
Query: 253 DVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRY 432
+VV+++ D + F+ P D F AG VE VGF N + + AS +
Sbjct: 29 EVVQATPTSDVS--FSYIFPDNQDKNF--AAGSVVEVLVGFTNNADKALNITHIFASLNH 84
Query: 433 PMDYTYYIQNFTALPYNREVKPKQEAPLPIPSSPMKLL 546
P D++ +IQN+T + V+ A L P + L
Sbjct: 85 PQDFSVFIQNYTRGDFGIAVEKGHHATLAYRFVPSEYL 122
>UniRef50_Q86FE2 Cluster: Clone ZZD1494 mRNA sequence; n=2;
Schistosoma japonicum|Rep: Clone ZZD1494 mRNA sequence -
Schistosoma japonicum (Blood fluke)
Length = 269
Score = 51.2 bits (117), Expect = 3e-05
Identities = 22/57 (38%), Positives = 33/57 (57%)
Frame = +3
Query: 519 YSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFFLYV 689
Y F P+ AGR F L++ + Y D + Y ++NQT+N+ E EG+D E FL +
Sbjct: 141 YRFKPSPELAGRSFDLSIVVYYHDNNNIYYSHKLFNQTVNLFETEEGVDTELIFLVI 197
Score = 35.5 bits (78), Expect = 1.4
Identities = 25/93 (26%), Positives = 38/93 (40%), Gaps = 5/93 (5%)
Frame = +1
Query: 253 DVVKSSQDIDTTILFTKPVPSLGDLTFDIQ--AGYPVEFFVGFINKGSVD---YVVESME 417
DV K S + + F+KP L ++ AG N D + ++ +E
Sbjct: 47 DVYKGSPAVSVLVAFSKPAYGLYSARREMNFPAGEVSSLVASLANTNPPDKEQFRLDFIE 106
Query: 418 ASFRYPMDYTYYIQNFTALPYNREVKPKQEAPL 516
+ YP Y Y +QNFT ++P QE L
Sbjct: 107 GALHYPSYYDYRMQNFTRQRLQGTLEPGQEISL 139
>UniRef50_Q4T5B3 Cluster: Chromosome undetermined SCAF9335, whole
genome shotgun sequence; n=8; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF9335,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 219
Score = 38.3 bits (85), Expect = 0.21
Identities = 17/33 (51%), Positives = 20/33 (60%)
Frame = +3
Query: 507 STFAYSFIPNEAFAGRPFGLNVQLNYRDASGNA 605
+TF SFIP E G PFGL + LNY D + A
Sbjct: 17 ATFECSFIPAEPMGGAPFGLVINLNYEDGAVRA 49
>UniRef50_A4RX78 Cluster: Predicted protein; n=2; Ostreococcus|Rep:
Predicted protein - Ostreococcus lucimarinus CCE9901
Length = 249
Score = 37.9 bits (84), Expect = 0.27
Identities = 23/87 (26%), Positives = 42/87 (48%), Gaps = 1/87 (1%)
Frame = +1
Query: 253 DVVKSSQDIDTTILFTKPVPSLGDLTFDIQAGYPVEFFVGFINKGSVDYVVESMEASFRY 432
D+ ++ D+++ + F P D F G V+ +G N G+ V + S
Sbjct: 31 DIPAANPDVESVVAF----PDHADRAFP--GGERVKVLLGLRNTGNDAVNVTHLAGSLNV 84
Query: 433 PMDYTYYIQNFTALPY-NREVKPKQEA 510
P ++ +Y+ NFT + + +KPK+EA
Sbjct: 85 PNNFNFYVTNFTVSSFGDAVIKPKKEA 111
Score = 33.1 bits (72), Expect = 7.7
Identities = 19/64 (29%), Positives = 30/64 (46%)
Frame = +3
Query: 498 KTGSTFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETF 677
K +TF Y F + FAG L + Y D +G AY +N T+ +++ D E
Sbjct: 108 KKEATFEYEFAIDPQFAGHSLQLALTAFY-DEAGVAYATTYFNSTVPVLDPKVVFDRELA 166
Query: 678 FLYV 689
+Y+
Sbjct: 167 VIYL 170
>UniRef50_P45434 Cluster: Translocon-associated protein subunit
alpha precursor; n=9; Magnoliophyta|Rep:
Translocon-associated protein subunit alpha precursor -
Arabidopsis thaliana (Mouse-ear cress)
Length = 258
Score = 36.3 bits (80), Expect = 0.83
Identities = 21/59 (35%), Positives = 28/59 (47%)
Frame = +3
Query: 507 STFAYSFIPNEAFAGRPFGLNVQLNYRDASGNAYQEAVYNQTLNIVEVSEGLDGETFFL 683
+TF Y F ++ F L + Y D G YQ YN T+ +VE L GE+ FL
Sbjct: 136 ATFPYIFAVSQYLQPGAFDLVGYIIY-DVEGKPYQSVFYNGTIEVVESGGLLSGESVFL 193
Score = 33.9 bits (74), Expect = 4.4
Identities = 18/54 (33%), Positives = 23/54 (42%)
Frame = +1
Query: 337 IQAGYPVEFFVGFINKGSVDYVVESMEASFRYPMDYTYYIQNFTALPYNREVKP 498
+ AG E VG N+G V + AS P D+ +QN T L N P
Sbjct: 78 VPAGEETELLVGLKNEGKTRVGVMGIRASVHLPYDHKLLVQNLTMLRLNNASIP 131
>UniRef50_Q54VL8 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 580
Score = 35.1 bits (77), Expect = 1.9
Identities = 23/75 (30%), Positives = 36/75 (48%)
Frame = +1
Query: 478 YNREVKPKQEAPLPIPSSPMKLLLEDLSD*MCSLITEMPAEMLTRKQSTTRHSTLWKYPK 657
Y+R+VK K+E + IP S + LL ++ + P++ L S + +WK P
Sbjct: 208 YDRDVKMKEEDSIEIPDSQVTTLLGHSNE--VFICAWNPSQSLLASGSGDSTARIWKIPP 265
Query: 658 V*MERHSSYMSSLVL 702
E +SSLVL
Sbjct: 266 GPCENSKEQVSSLVL 280
>UniRef50_Q24U67 Cluster: Putative uncharacterized protein; n=2;
Desulfitobacterium hafniense|Rep: Putative
uncharacterized protein - Desulfitobacterium hafniense
(strain Y51)
Length = 143
Score = 33.5 bits (73), Expect = 5.8
Identities = 19/54 (35%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
Frame = +1
Query: 493 KPKQEAPLPIPSSPMKLLLEDLSD*MCSLITEMPA-EMLTRKQSTTRHSTLWKY 651
K +Q PLPIPS+P + L D SD + + +P + +S T++ TL K+
Sbjct: 57 KEEQALPLPIPSAPEDITLYDASDRIVFIEVYLPPYRNEAKNKSVTKNCTLPKW 110
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 718,905,565
Number of Sequences: 1657284
Number of extensions: 14335486
Number of successful extensions: 35081
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 33924
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 35070
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 63381147830
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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