Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0238
(723 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 117 9e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 117 9e-27
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 31 0.77
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 30 1.8
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.4
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.1
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.1
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 29 3.1
At1g21390.1 68414.m02676 expressed protein 29 3.1
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 4.1
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 5.5
At4g08960.1 68417.m01476 phosphotyrosyl phosphatase activator (P... 28 7.2
At1g18670.1 68414.m02330 protein kinase family protein contains ... 28 7.2
At4g38225.3 68417.m05398 expressed protein 27 9.5
At4g38225.2 68417.m05396 expressed protein 27 9.5
At4g38225.1 68417.m05397 expressed protein 27 9.5
At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related... 27 9.5
At1g76010.1 68414.m08825 expressed protein 27 9.5
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 27 9.5
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 27 9.5
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 117 bits (281), Expect = 9e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 126
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPIFP 527
ARGH IE P P
Sbjct: 127 RHAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 72.1 bits (169), Expect = 3e-13
Identities = 31/68 (45%), Positives = 46/68 (67%)
Frame = +2
Query: 512 IPDLPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 691
+P++PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 212
Query: 692 PLIIFNKD 715
PL++F +
Sbjct: 213 PLVVFGTE 220
Score = 66.1 bits (154), Expect = 2e-11
Identities = 33/63 (52%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +1
Query: 76 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 249
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 250 GHK 258
GH+
Sbjct: 65 GHQ 67
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 117 bits (281), Expect = 9e-27
Identities = 55/91 (60%), Positives = 57/91 (62%)
Frame = +3
Query: 255 QTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXX 434
QTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 QTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMK 125
Query: 435 XXXXXXXXXXXXXXXXXQARGHIIERFPIFP 527
ARGH IE P P
Sbjct: 126 RHAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 70.9 bits (166), Expect = 8e-13
Identities = 30/68 (44%), Positives = 46/68 (67%)
Frame = +2
Query: 512 IPDLPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 691
+P++PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 211
Query: 692 PLIIFNKD 715
PL+++ +
Sbjct: 212 PLVVYGTE 219
Score = 68.1 bits (159), Expect = 6e-12
Identities = 33/66 (50%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +1
Query: 67 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 240
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 241 KEAGHK 258
K+AGH+
Sbjct: 61 KKAGHQ 66
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 31.1 bits (67), Expect = 0.77
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -1
Query: 369 HHDTCYRRHPDRTYGYHHHGHAEF 298
HH Y H YGY +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 29.9 bits (64), Expect = 1.8
Identities = 16/64 (25%), Positives = 32/64 (50%)
Frame = -3
Query: 697 EGSLTLDTTTVAHFTLTSTKTLRLVHFKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKI 518
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + KI
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 517 GNLS 506
G ++
Sbjct: 302 GEIN 305
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +1
Query: 91 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 29.1 bits (62), Expect = 3.1
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 365 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 463
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 29.1 bits (62), Expect = 3.1
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +2
Query: 365 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 463
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/25 (56%), Positives = 17/25 (68%)
Frame = -1
Query: 570 CLVLLISWTLSATTKGRSGIFQ*CV 496
CLVLL+S +A TK SGIF C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At1g21390.1 68414.m02676 expressed protein
Length = 248
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -2
Query: 524 EDRESFNNVSSSLNERWDASSSNGCCQGRSPLSE 423
ED+E +N +S S +R +SSSN + RS L +
Sbjct: 185 EDKEWWNRMSESSTKRSGSSSSNNSIRSRSSLRD 218
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.7 bits (61), Expect = 4.1
Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 3/64 (4%)
Frame = +3
Query: 216 LEAALLREQGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 386
+ AL+R + G ++ +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMG-AKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 387 TKPW 398
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 9.5
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 142 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 237
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At4g08960.1 68417.m01476 phosphotyrosyl phosphatase activator
(PTPA) family protein similar to Protein phosphatase 2A,
regulatory subunit B' (PP2A, subunit B', PR53 isoform)
(Phosphotyrosyl phosphatase activator) (PTPA)
(Swiss-Prot:Q28717) [Oryctolagus cuniculus]
Length = 392
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/36 (41%), Positives = 20/36 (55%)
Frame = -3
Query: 352 PKAP*PDLWVPPPRTRGIRATARPVPHDSALVCDQP 245
P A P++ PPP R IRA A +PH+S + P
Sbjct: 46 PLASFPEM-SPPPNYRPIRAPAINLPHNSQAIILSP 80
>At1g18670.1 68414.m02330 protein kinase family protein contains
Protein kinases ATP-binding region signature,
PROSITE:PS00107 and Serine/Threonine protein kinases
active-site signature, PROSITE:PS00108
Length = 662
Score = 27.9 bits (59), Expect = 7.2
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = +1
Query: 511 DSRSSLGCSRQSPGDQQDQTGCHLPEAPQGMV*YP*SVQVSASS 642
DS S+L Q P + + H+ A QG V + +QVS SS
Sbjct: 501 DSDSTLFEKMQKPSNHEKDEASHVKNASQGDVPFSGPLQVSVSS 544
>At4g38225.3 68417.m05398 expressed protein
Length = 365
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/47 (25%), Positives = 25/47 (53%)
Frame = -2
Query: 545 LCRLQPREDRESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVP 405
L + QP D ++ + +++ WD ++ C+G+ PL ++ VP
Sbjct: 62 LAQSQPAPDFNPWSEFAQNVSGEWDGFGADFTCEGQ-PLELPESVVP 107
>At4g38225.2 68417.m05396 expressed protein
Length = 308
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/47 (25%), Positives = 25/47 (53%)
Frame = -2
Query: 545 LCRLQPREDRESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVP 405
L + QP D ++ + +++ WD ++ C+G+ PL ++ VP
Sbjct: 62 LAQSQPAPDFNPWSEFAQNVSGEWDGFGADFTCEGQ-PLELPESVVP 107
>At4g38225.1 68417.m05397 expressed protein
Length = 363
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/47 (25%), Positives = 25/47 (53%)
Frame = -2
Query: 545 LCRLQPREDRESFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVP 405
L + QP D ++ + +++ WD ++ C+G+ PL ++ VP
Sbjct: 62 LAQSQPAPDFNPWSEFAQNVSGEWDGFGADFTCEGQ-PLELPESVVP 107
>At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related
protein, putative similar to kinesin like protein
GB:CAB10194 from [Arabidopsis thaliana]
Length = 1313
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 515 ESFNNVSSSLNERWDASSSNGCCQGRSPLSEVD 417
ES+NN +E D + + CCQ + SE D
Sbjct: 557 ESYNNSHLKSSEATDVNMEDACCQTENNGSETD 589
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 27.5 bits (58), Expect = 9.5
Identities = 17/43 (39%), Positives = 18/43 (41%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 368
QG + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +3
Query: 240 QGGWSQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 374
+G S+ + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,548,501
Number of Sequences: 28952
Number of extensions: 340483
Number of successful extensions: 1229
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1219
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1575119672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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