SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0237
         (713 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g21630.1 68414.m02708 calcium-binding EF hand family protein ...    47   1e-05
At1g20760.1 68414.m02600 calcium-binding EF hand family protein ...    46   3e-05
At3g20290.1 68416.m02571 calcium-binding EF hand family protein ...    37   0.015
At1g49890.1 68414.m05593 expressed protein contains Pfam domain,...    31   0.57 
At5g04020.1 68418.m00382 calmodulin-binding protein-related (PIC...    28   5.3  
At4g03410.2 68417.m00465 peroxisomal membrane protein-related co...    28   5.3  
At3g46580.1 68416.m05056 methyl-CpG-binding domain-containing pr...    28   7.1  
At3g19570.2 68416.m02482 expressed protein contains Pfam domain,...    28   7.1  
At3g19570.1 68416.m02481 expressed protein contains Pfam domain,...    28   7.1  
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p...    27   9.3  
At1g03870.1 68414.m00371 fasciclin-like arabinogalactan-protein ...    27   9.3  

>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
           contains INTERPRO:IPR002048 calcium-binding EF-hand
           domain; ESTs gb|T44428 and gb|AA395440 come from this
           gene
          Length = 1218

 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 31/84 (36%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
 Frame = +3

Query: 75  ISEAQAHHYASQFSQLRPER-GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSL 251
           ++ A    Y   F Q+  +R G ++G  AR  F   RLP   L+++W LSD D D MLSL
Sbjct: 420 MTPADVQKYTKVFVQVDTDRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSL 479

Query: 252 ESSA*PCISLYCGGTIFRYRRCYP 323
                 CI++Y    + RYR   P
Sbjct: 480 REF---CIAVY---LMERYREGRP 497



 Score = 30.7 bits (66), Expect = 1.0
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +3

Query: 135 GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGML 245
           G +SG  A  FF+ S LP   L ++W  +D  K G L
Sbjct: 26  GHISGAEAVAFFQGSNLPKHVLAQVWSYADSKKAGYL 62


>At1g20760.1 68414.m02600 calcium-binding EF hand family protein
           contains INTERPRO:IPR002048 calcium-binding EF-hand
           domain
          Length = 1019

 Score = 45.6 bits (103), Expect = 3e-05
 Identities = 30/76 (39%), Positives = 41/76 (53%), Gaps = 1/76 (1%)
 Frame = +3

Query: 99  YASQFSQLRPER-GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSLESSA*PCI 275
           Y   F ++  ++ G ++G+ AR  F   RLP   L+ +W+LSD D D MLSL      CI
Sbjct: 363 YTKVFMEVDSDKDGKITGEQARNLFLSWRLPREVLKHVWELSDQDNDTMLSLREF---CI 419

Query: 276 SLYCGGTIFRYRRCYP 323
           SLY    + RYR   P
Sbjct: 420 SLY---LMERYREGRP 432



 Score = 29.1 bits (62), Expect = 3.1
 Identities = 16/41 (39%), Positives = 21/41 (51%)
 Frame = +3

Query: 135 GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSLES 257
           G +SG  A  FF+ S L    L +IW LSD    G L  ++
Sbjct: 24  GRISGAEAVGFFQGSGLSKQVLAQIWSLSDRSHSGFLDRQN 64


>At3g20290.1 68416.m02571 calcium-binding EF hand family protein
           similar to EH-domain containing protein 1 from {Mus
           musculus} SP|Q9WVK4 and {Homo sapiens} SP|Q9H4M9,
           receptor-mediated endocytosis 1 from [Caenorhabditis
           elegans] GI:13487775, GI:13487777, GI:13487779; contains
           INTERPRO:IPR002048 calcium-binding EF-hand domain
          Length = 545

 Score = 36.7 bits (81), Expect = 0.015
 Identities = 15/40 (37%), Positives = 24/40 (60%)
 Frame = +3

Query: 135 GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSLE 254
           G ++G  A  FF  S LP P+L++IW ++D  + G L  +
Sbjct: 32  GRITGNDAIKFFTMSNLPRPELKQIWAIADSKRQGYLGFK 71


>At1g49890.1 68414.m05593 expressed protein contains Pfam domain,
           PF04484: Family of unknown function (DUF566)
          Length = 659

 Score = 31.5 bits (68), Expect = 0.57
 Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 3/70 (4%)
 Frame = +3

Query: 393 APRCSARGLQQTSISHR---NRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFV 563
           +P  S+ GL+ +SIS +   ++  +          G+ S +RG A ++ A P+K W    
Sbjct: 346 SPLSSSPGLKTSSISSKFGLSKRFSSDAVPLSSPRGMASPVRGSAIRS-ASPSKLWATTT 404

Query: 564 DSPTSSVSSP 593
            SP  ++SSP
Sbjct: 405 SSPARALSSP 414


>At5g04020.1 68418.m00382 calmodulin-binding protein-related (PICBP)
           contains similarity to potato calmodulin-binding protein
           PCBP GI:17933110 from [Solanum tuberosum]
          Length = 1495

 Score = 28.3 bits (60), Expect = 5.3
 Identities = 17/47 (36%), Positives = 25/47 (53%)
 Frame = -2

Query: 268 GYAELSNESIPSLSMSDSCQIFLKSGTGSLDFSKNSLAVCPDRSPRS 128
           GY   S +S  S  MSD  + + KS   + +  + SL +C +R PRS
Sbjct: 61  GYTAESEDSSSS-EMSDDSRTYSKSSDENEELDQASLTICSER-PRS 105


>At4g03410.2 68417.m00465 peroxisomal membrane protein-related
           contains weak similarity to Swiss-Prot:P42925 22 kDa
           peroxisomal membrane protein [Mus musculus]
          Length = 361

 Score = 28.3 bits (60), Expect = 5.3
 Identities = 17/51 (33%), Positives = 24/51 (47%)
 Frame = -2

Query: 283 YNEMHGYAELSNESIPSLSMSDSCQIFLKSGTGSLDFSKNSLAVCPDRSPR 131
           Y+     A+ S E+  S   S+ CQ+FL S   S   S   L VC + S +
Sbjct: 295 YSNEKAEAQASEETNSSSHSSEVCQVFLFSKNLSSKISLERLNVCSECSSK 345


>At3g46580.1 68416.m05056 methyl-CpG-binding domain-containing
           protein weak similarity to SP|P51608 Methyl-CpG-binding
           protein 2 (MeCP-2 protein) (MeCP2) {Homo sapiens};
           contains Pfam profile PF01429: Methyl-CpG binding domain
          Length = 182

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = +3

Query: 582 VSSPGPKPVNFDFQRAP 632
           V+ P PKP+NFDF   P
Sbjct: 117 VTEPPPKPLNFDFLNVP 133


>At3g19570.2 68416.m02482 expressed protein contains Pfam domain,
           PF04484: Family of unknown function (DUF566)
          Length = 644

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
 Frame = +3

Query: 393 APRCSARGLQQTSISHR---NRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFV 563
           +P+CS+   + +SIS +   ++  +    +     G+ S IRG      A P+K W    
Sbjct: 343 SPQCSSPSSRISSISSKFSQSKRFSSDSPLTSSPRGMTSPIRGAT--RPASPSKLWATAT 400

Query: 564 DSPTSSVSSP 593
            +P  + SSP
Sbjct: 401 SAPARTSSSP 410


>At3g19570.1 68416.m02481 expressed protein contains Pfam domain,
           PF04484: Family of unknown function (DUF566)
          Length = 627

 Score = 27.9 bits (59), Expect = 7.1
 Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
 Frame = +3

Query: 393 APRCSARGLQQTSISHR---NRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFV 563
           +P+CS+   + +SIS +   ++  +    +     G+ S IRG      A P+K W    
Sbjct: 343 SPQCSSPSSRISSISSKFSQSKRFSSDSPLTSSPRGMTSPIRGAT--RPASPSKLWATAT 400

Query: 564 DSPTSSVSSP 593
            +P  + SSP
Sbjct: 401 SAPARTSSSP 410


>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
           putative similar to dihydrolipoamide acetyltransferase
           (E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
           contains Pfam profiles PF00198: 2-oxo acid
           dehydrogenases acyltransferase (catalytic domain),
           PF00364: Biotin-requiring enzyme, PF02817: e3 binding
           domain; supporting cDNA
           gi|5881964|gb|AF066080.1|AF066080
          Length = 637

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 22/92 (23%), Positives = 39/92 (42%)
 Frame = +3

Query: 414 GLQQTSISHRNRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFVDSPTSSVSSP 593
           GL+ +SI        L+++          A +  A     QP+KE      S +SS S P
Sbjct: 343 GLEASSIEASGPYGTLLKSDVVAAIASGKASKSSASTKKKQPSKE----TPSKSSSTSKP 398

Query: 594 GPKPVNFDFQRAPSNEIRKFFIRSRCGLHQKL 689
                + +++  P+++IRK   +      QK+
Sbjct: 399 SVTQSDNNYEDFPNSQIRKIIAKRLLESKQKI 430


>At1g03870.1 68414.m00371 fasciclin-like arabinogalactan-protein
           (FLA9) identical to gi_13377784_gb_AAK20861
          Length = 247

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 19/55 (34%), Positives = 27/55 (49%)
 Frame = +2

Query: 365 VVTTDLVDLGAEMFGSGTSADFNFAPKSDSGADPYDAKTSRRPEERHPRLRSKSG 529
           V   D+V L  EMFG    +    APKS SG    D+ ++++     P  +S SG
Sbjct: 174 VYVVDMVLLPGEMFGEHKLSPIAPAPKSKSGGVTDDSGSTKKAAS--PSDKSGSG 226


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,318,641
Number of Sequences: 28952
Number of extensions: 291925
Number of successful extensions: 1014
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 972
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1013
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -