BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0237 (713 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 47 1e-05 At1g20760.1 68414.m02600 calcium-binding EF hand family protein ... 46 3e-05 At3g20290.1 68416.m02571 calcium-binding EF hand family protein ... 37 0.015 At1g49890.1 68414.m05593 expressed protein contains Pfam domain,... 31 0.57 At5g04020.1 68418.m00382 calmodulin-binding protein-related (PIC... 28 5.3 At4g03410.2 68417.m00465 peroxisomal membrane protein-related co... 28 5.3 At3g46580.1 68416.m05056 methyl-CpG-binding domain-containing pr... 28 7.1 At3g19570.2 68416.m02482 expressed protein contains Pfam domain,... 28 7.1 At3g19570.1 68416.m02481 expressed protein contains Pfam domain,... 28 7.1 At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 27 9.3 At1g03870.1 68414.m00371 fasciclin-like arabinogalactan-protein ... 27 9.3 >At1g21630.1 68414.m02708 calcium-binding EF hand family protein contains INTERPRO:IPR002048 calcium-binding EF-hand domain; ESTs gb|T44428 and gb|AA395440 come from this gene Length = 1218 Score = 47.2 bits (107), Expect = 1e-05 Identities = 31/84 (36%), Positives = 43/84 (51%), Gaps = 1/84 (1%) Frame = +3 Query: 75 ISEAQAHHYASQFSQLRPER-GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSL 251 ++ A Y F Q+ +R G ++G AR F RLP L+++W LSD D D MLSL Sbjct: 420 MTPADVQKYTKVFVQVDTDRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSL 479 Query: 252 ESSA*PCISLYCGGTIFRYRRCYP 323 CI++Y + RYR P Sbjct: 480 REF---CIAVY---LMERYREGRP 497 Score = 30.7 bits (66), Expect = 1.0 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = +3 Query: 135 GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGML 245 G +SG A FF+ S LP L ++W +D K G L Sbjct: 26 GHISGAEAVAFFQGSNLPKHVLAQVWSYADSKKAGYL 62 >At1g20760.1 68414.m02600 calcium-binding EF hand family protein contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 1019 Score = 45.6 bits (103), Expect = 3e-05 Identities = 30/76 (39%), Positives = 41/76 (53%), Gaps = 1/76 (1%) Frame = +3 Query: 99 YASQFSQLRPER-GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSLESSA*PCI 275 Y F ++ ++ G ++G+ AR F RLP L+ +W+LSD D D MLSL CI Sbjct: 363 YTKVFMEVDSDKDGKITGEQARNLFLSWRLPREVLKHVWELSDQDNDTMLSLREF---CI 419 Query: 276 SLYCGGTIFRYRRCYP 323 SLY + RYR P Sbjct: 420 SLY---LMERYREGRP 432 Score = 29.1 bits (62), Expect = 3.1 Identities = 16/41 (39%), Positives = 21/41 (51%) Frame = +3 Query: 135 GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSLES 257 G +SG A FF+ S L L +IW LSD G L ++ Sbjct: 24 GRISGAEAVGFFQGSGLSKQVLAQIWSLSDRSHSGFLDRQN 64 >At3g20290.1 68416.m02571 calcium-binding EF hand family protein similar to EH-domain containing protein 1 from {Mus musculus} SP|Q9WVK4 and {Homo sapiens} SP|Q9H4M9, receptor-mediated endocytosis 1 from [Caenorhabditis elegans] GI:13487775, GI:13487777, GI:13487779; contains INTERPRO:IPR002048 calcium-binding EF-hand domain Length = 545 Score = 36.7 bits (81), Expect = 0.015 Identities = 15/40 (37%), Positives = 24/40 (60%) Frame = +3 Query: 135 GLLSGQTARLFFEKSRLPVPDLRKIWQLSDIDKDGMLSLE 254 G ++G A FF S LP P+L++IW ++D + G L + Sbjct: 32 GRITGNDAIKFFTMSNLPRPELKQIWAIADSKRQGYLGFK 71 >At1g49890.1 68414.m05593 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 659 Score = 31.5 bits (68), Expect = 0.57 Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 3/70 (4%) Frame = +3 Query: 393 APRCSARGLQQTSISHR---NRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFV 563 +P S+ GL+ +SIS + ++ + G+ S +RG A ++ A P+K W Sbjct: 346 SPLSSSPGLKTSSISSKFGLSKRFSSDAVPLSSPRGMASPVRGSAIRS-ASPSKLWATTT 404 Query: 564 DSPTSSVSSP 593 SP ++SSP Sbjct: 405 SSPARALSSP 414 >At5g04020.1 68418.m00382 calmodulin-binding protein-related (PICBP) contains similarity to potato calmodulin-binding protein PCBP GI:17933110 from [Solanum tuberosum] Length = 1495 Score = 28.3 bits (60), Expect = 5.3 Identities = 17/47 (36%), Positives = 25/47 (53%) Frame = -2 Query: 268 GYAELSNESIPSLSMSDSCQIFLKSGTGSLDFSKNSLAVCPDRSPRS 128 GY S +S S MSD + + KS + + + SL +C +R PRS Sbjct: 61 GYTAESEDSSSS-EMSDDSRTYSKSSDENEELDQASLTICSER-PRS 105 >At4g03410.2 68417.m00465 peroxisomal membrane protein-related contains weak similarity to Swiss-Prot:P42925 22 kDa peroxisomal membrane protein [Mus musculus] Length = 361 Score = 28.3 bits (60), Expect = 5.3 Identities = 17/51 (33%), Positives = 24/51 (47%) Frame = -2 Query: 283 YNEMHGYAELSNESIPSLSMSDSCQIFLKSGTGSLDFSKNSLAVCPDRSPR 131 Y+ A+ S E+ S S+ CQ+FL S S S L VC + S + Sbjct: 295 YSNEKAEAQASEETNSSSHSSEVCQVFLFSKNLSSKISLERLNVCSECSSK 345 >At3g46580.1 68416.m05056 methyl-CpG-binding domain-containing protein weak similarity to SP|P51608 Methyl-CpG-binding protein 2 (MeCP-2 protein) (MeCP2) {Homo sapiens}; contains Pfam profile PF01429: Methyl-CpG binding domain Length = 182 Score = 27.9 bits (59), Expect = 7.1 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = +3 Query: 582 VSSPGPKPVNFDFQRAP 632 V+ P PKP+NFDF P Sbjct: 117 VTEPPPKPLNFDFLNVP 133 >At3g19570.2 68416.m02482 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 644 Score = 27.9 bits (59), Expect = 7.1 Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 3/70 (4%) Frame = +3 Query: 393 APRCSARGLQQTSISHR---NRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFV 563 +P+CS+ + +SIS + ++ + + G+ S IRG A P+K W Sbjct: 343 SPQCSSPSSRISSISSKFSQSKRFSSDSPLTSSPRGMTSPIRGAT--RPASPSKLWATAT 400 Query: 564 DSPTSSVSSP 593 +P + SSP Sbjct: 401 SAPARTSSSP 410 >At3g19570.1 68416.m02481 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 627 Score = 27.9 bits (59), Expect = 7.1 Identities = 19/70 (27%), Positives = 33/70 (47%), Gaps = 3/70 (4%) Frame = +3 Query: 393 APRCSARGLQQTSISHR---NRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFV 563 +P+CS+ + +SIS + ++ + + G+ S IRG A P+K W Sbjct: 343 SPQCSSPSSRISSISSKFSQSKRFSSDSPLTSSPRGMTSPIRGAT--RPASPSKLWATAT 400 Query: 564 DSPTSSVSSP 593 +P + SSP Sbjct: 401 SAPARTSSSP 410 >At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide acetyltransferase (E2) subunit of PDC [Arabidopsis thaliana] GI:559395; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain; supporting cDNA gi|5881964|gb|AF066080.1|AF066080 Length = 637 Score = 27.5 bits (58), Expect = 9.3 Identities = 22/92 (23%), Positives = 39/92 (42%) Frame = +3 Query: 414 GLQQTSISHRNRTVALIRTMPKRVAGLRSAIRGFAPKADAQPNKEWTKFVDSPTSSVSSP 593 GL+ +SI L+++ A + A QP+KE S +SS S P Sbjct: 343 GLEASSIEASGPYGTLLKSDVVAAIASGKASKSSASTKKKQPSKE----TPSKSSSTSKP 398 Query: 594 GPKPVNFDFQRAPSNEIRKFFIRSRCGLHQKL 689 + +++ P+++IRK + QK+ Sbjct: 399 SVTQSDNNYEDFPNSQIRKIIAKRLLESKQKI 430 >At1g03870.1 68414.m00371 fasciclin-like arabinogalactan-protein (FLA9) identical to gi_13377784_gb_AAK20861 Length = 247 Score = 27.5 bits (58), Expect = 9.3 Identities = 19/55 (34%), Positives = 27/55 (49%) Frame = +2 Query: 365 VVTTDLVDLGAEMFGSGTSADFNFAPKSDSGADPYDAKTSRRPEERHPRLRSKSG 529 V D+V L EMFG + APKS SG D+ ++++ P +S SG Sbjct: 174 VYVVDMVLLPGEMFGEHKLSPIAPAPKSKSGGVTDDSGSTKKAAS--PSDKSGSG 226 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,318,641 Number of Sequences: 28952 Number of extensions: 291925 Number of successful extensions: 1014 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 972 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1013 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1545769616 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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