Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0232
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,... 127 6e-30
At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) ... 104 5e-23
At5g04140.2 68418.m00402 glutamate synthase (GLU1) / ferredoxin-... 102 2e-22
At5g04140.1 68418.m00401 glutamate synthase (GLU1) / ferredoxin-... 102 2e-22
At2g27600.1 68415.m03346 AAA-type ATPase family protein / vacuol... 29 2.8
At4g25730.1 68417.m03703 FtsJ-like methyltransferase family prot... 29 3.8
At1g54390.4 68414.m06205 PHD finger protein-related contains low... 29 3.8
At1g54390.3 68414.m06204 PHD finger protein-related contains low... 29 3.8
At1g54390.2 68414.m06202 PHD finger protein-related contains low... 29 3.8
At1g54390.1 68414.m06203 PHD finger protein-related contains low... 29 3.8
At4g31980.1 68417.m04547 expressed protein contains Pfam profile... 28 6.6
At5g17890.1 68418.m02098 LIM domain-containing protein / disease... 27 8.7
>At5g53460.1 68418.m06644 glutamate synthase [NADH], chloroplast,
putative similar to SP|Q03460 Glutamate synthase [NADH],
chloroplast precursor (EC 1.4.1.14) (NADH- GOGAT)
{Medicago sativa}
Length = 2208
Score = 127 bits (307), Expect = 6e-30
Identities = 56/84 (66%), Positives = 66/84 (78%)
Frame = +2
Query: 2 DYVGKGLSGGTVVIYPPRDSPFESHLNVIVGNVCLYGATSGRAYFRGIASERFCVRNSSC 181
DYVGKGLSGG VV+YPP+ S F+ N+++GNV LYGATSG AYF G+A+ERF VRNS
Sbjct: 1461 DYVGKGLSGGKVVVYPPKGSSFDPKENIVIGNVALYGATSGEAYFNGMAAERFSVRNSGA 1520
Query: 182 TAVVEVVGDHACEYMTXGAALVLG 253
AVVE +GDH CEYMT G +VLG
Sbjct: 1521 KAVVEGLGDHGCEYMTGGTVVVLG 1544
Score = 60.5 bits (140), Expect = 1e-09
Identities = 28/76 (36%), Positives = 43/76 (56%)
Frame = +1
Query: 280 MSGGIAYVYDIDGSFKGKCNPEMVXXXXXXXXXXXKYVQKLLEEFVEYTGSLIAVELLKT 459
MSGGIAYV D+DG F +CN E+V ++ ++++ +T S +A E+L
Sbjct: 1554 MSGGIAYVLDVDGKFNTRCNLELVDLDKVEDEEDKMTLKMMIQQHQRHTNSQLAQEVLAD 1613
Query: 460 WPEPAKKFVKVFPYEY 507
+ KF+KVFP +Y
Sbjct: 1614 FENLLPKFIKVFPRDY 1629
>At2g41220.1 68415.m05090 glutamate synthase, chloroplast (GLU2) /
ferredoxin-dependent glutamate synthase (Fd-GOGAT 2)
identical to SP|Q9T0P4 Ferredoxin-dependent glutamate
synthase 2, chloroplast precursor (EC 1.4.7.1) (Fd-GOGAT
2) {Arabidopsis thaliana}
Length = 1629
Score = 104 bits (250), Expect = 5e-23
Identities = 49/84 (58%), Positives = 55/84 (65%)
Frame = +2
Query: 2 DYVGKGLSGGTVVIYPPRDSPFESHLNVIVGNVCLYGATSGRAYFRGIASERFCVRNSSC 181
DYVGKG++GG VVI P + F IVGN CLYGAT G + RG A ERF VRNS
Sbjct: 1432 DYVGKGMAGGEVVILPVESTGFRPEDATIVGNTCLYGATGGLLFVRGKAGERFAVRNSLA 1491
Query: 182 TAVVEVVGDHACEYMTXGAALVLG 253
AVVE GDH CEYMT G ++LG
Sbjct: 1492 QAVVEGTGDHCCEYMTGGCVVILG 1515
Score = 39.1 bits (87), Expect = 0.003
Identities = 20/73 (27%), Positives = 35/73 (47%)
Frame = +1
Query: 280 MSGGIAYVYDIDGSFKGKCNPEMVXXXXXXXXXXXKYVQKLLEEFVEYTGSLIAVELLKT 459
M+GG+AY+ D D + K N E+V ++ L++ VE TGS +++
Sbjct: 1525 MTGGLAYILDEDNTLLPKMNKEIVKIQRVTSPVGQTQLKSLIQAHVEKTGSSKGAMIVEE 1584
Query: 460 WPEPAKKFVKVFP 498
W + F ++ P
Sbjct: 1585 WDKYLAMFWQLVP 1597
>At5g04140.2 68418.m00402 glutamate synthase (GLU1) /
ferredoxin-dependent glutamate synthase (Fd-GOGAT 1)
identical to ferredoxin-dependent glutamate synthase
precursor [Arabidopsis thaliana] GI:3869251
Length = 1648
Score = 102 bits (245), Expect = 2e-22
Identities = 48/84 (57%), Positives = 55/84 (65%)
Frame = +2
Query: 2 DYVGKGLSGGTVVIYPPRDSPFESHLNVIVGNVCLYGATSGRAYFRGIASERFCVRNSSC 181
DYVGKG++GG +V+ P F IVGN CLYGAT G+ + RG A ERF VRNS
Sbjct: 1457 DYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGATGGQIFARGKAGERFAVRNSLA 1516
Query: 182 TAVVEVVGDHACEYMTXGAALVLG 253
AVVE GDH CEYMT G +VLG
Sbjct: 1517 EAVVEGTGDHCCEYMTGGCVVVLG 1540
Score = 39.1 bits (87), Expect = 0.003
Identities = 22/73 (30%), Positives = 34/73 (46%)
Frame = +1
Query: 280 MSGGIAYVYDIDGSFKGKCNPEMVXXXXXXXXXXXKYVQKLLEEFVEYTGSLIAVELLKT 459
M+GG+AY+ D D + K N E+V ++ L+E VE TGS +L
Sbjct: 1550 MTGGLAYLLDEDDTLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNE 1609
Query: 460 WPEPAKKFVKVFP 498
W + F ++ P
Sbjct: 1610 WEKYLPLFWQLVP 1622
>At5g04140.1 68418.m00401 glutamate synthase (GLU1) /
ferredoxin-dependent glutamate synthase (Fd-GOGAT 1)
identical to ferredoxin-dependent glutamate synthase
precursor [Arabidopsis thaliana] GI:3869251
Length = 1622
Score = 102 bits (245), Expect = 2e-22
Identities = 48/84 (57%), Positives = 55/84 (65%)
Frame = +2
Query: 2 DYVGKGLSGGTVVIYPPRDSPFESHLNVIVGNVCLYGATSGRAYFRGIASERFCVRNSSC 181
DYVGKG++GG +V+ P F IVGN CLYGAT G+ + RG A ERF VRNS
Sbjct: 1431 DYVGKGMAGGEIVVTPVEKIGFVPEEATIVGNTCLYGATGGQIFARGKAGERFAVRNSLA 1490
Query: 182 TAVVEVVGDHACEYMTXGAALVLG 253
AVVE GDH CEYMT G +VLG
Sbjct: 1491 EAVVEGTGDHCCEYMTGGCVVVLG 1514
Score = 39.1 bits (87), Expect = 0.003
Identities = 22/73 (30%), Positives = 34/73 (46%)
Frame = +1
Query: 280 MSGGIAYVYDIDGSFKGKCNPEMVXXXXXXXXXXXKYVQKLLEEFVEYTGSLIAVELLKT 459
M+GG+AY+ D D + K N E+V ++ L+E VE TGS +L
Sbjct: 1524 MTGGLAYLLDEDDTLLPKINREIVKIQRVTAPAGELQLKSLIEAHVEKTGSSKGATILNE 1583
Query: 460 WPEPAKKFVKVFP 498
W + F ++ P
Sbjct: 1584 WEKYLPLFWQLVP 1596
>At2g27600.1 68415.m03346 AAA-type ATPase family protein / vacuolar
sorting protein-related similar to SP|P46467 SKD1
protein (Vacuolar sorting protein 4b) {Mus musculus};
contains Pfam profiles PF00004: ATPase AAA family,
PF04212: MIT domain
Length = 435
Score = 29.1 bits (62), Expect = 2.8
Identities = 18/54 (33%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = +2
Query: 236 AALVLGPPA-GTSRPA*AVASPTSTTLMVHSKGNVILKWLNSYLWNSKTTSNMY 394
A L+ GPP G S A AVA+ +T S +++ KW+ + K SN++
Sbjct: 167 AFLLYGPPGTGKSYLAKAVATEADSTFFSVSSSDLVSKWMGE---SEKLVSNLF 217
>At4g25730.1 68417.m03703 FtsJ-like methyltransferase family protein
contains Pfam profile: PF01728 FtsJ-like
methyltransferase
Length = 821
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 519 KQMALKQPAAQKGDTNGHQNGVL-DIEETVKDVEFEKKNLEKILDKTR 659
K++A +P K D + +L ++EE V+ +KK +KIL K R
Sbjct: 330 KEVAKPEPDVGKEDEENEDDKLLNELEELTNTVDRKKKQAKKILAKRR 377
>At1g54390.4 68414.m06205 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 262
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 4/40 (10%)
Frame = +3
Query: 525 MALKQPAAQKGDTNGHQNGVLDIEETV----KDVEFEKKN 632
+ L +++KG+ N + NG LD EET+ K++E ++N
Sbjct: 53 LGLASQSSKKGNGNHYNNGGLDEEETIEKMRKEIESSQEN 92
>At1g54390.3 68414.m06204 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 262
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 4/40 (10%)
Frame = +3
Query: 525 MALKQPAAQKGDTNGHQNGVLDIEETV----KDVEFEKKN 632
+ L +++KG+ N + NG LD EET+ K++E ++N
Sbjct: 53 LGLASQSSKKGNGNHYNNGGLDEEETIEKMRKEIESSQEN 92
>At1g54390.2 68414.m06202 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 328
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 4/40 (10%)
Frame = +3
Query: 525 MALKQPAAQKGDTNGHQNGVLDIEETV----KDVEFEKKN 632
+ L +++KG+ N + NG LD EET+ K++E ++N
Sbjct: 53 LGLASQSSKKGNGNHYNNGGLDEEETIEKMRKEIESSQEN 92
>At1g54390.1 68414.m06203 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 306
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/40 (35%), Positives = 25/40 (62%), Gaps = 4/40 (10%)
Frame = +3
Query: 525 MALKQPAAQKGDTNGHQNGVLDIEETV----KDVEFEKKN 632
+ L +++KG+ N + NG LD EET+ K++E ++N
Sbjct: 53 LGLASQSSKKGNGNHYNNGGLDEEETIEKMRKEIESSQEN 92
>At4g31980.1 68417.m04547 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 680
Score = 27.9 bits (59), Expect = 6.6
Identities = 15/41 (36%), Positives = 24/41 (58%)
Frame = +2
Query: 287 VASPTSTTLMVHSKGNVILKWLNSYLWNSKTTSNMYRNFSK 409
+ SPT L++HS G ++ +YL NS SN++ + SK
Sbjct: 579 IKSPTDADLLIHS-GIIV-----NYLGNSVDVSNLFNSISK 613
>At5g17890.1 68418.m02098 LIM domain-containing protein / disease
resistance protein-related low similarity to disease
resistance protein RPP4 [Arabidopsis thaliana]
GI:20270890; contains Pfam profiles PF00412: LIM domain,
PF00931: NB-ARC domain, PF00560: Leucine Rich Repeat
Length = 1613
Score = 27.5 bits (58), Expect = 8.7
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +2
Query: 269 SRPA*AVASPTSTTLMVHSKGNVILKWLNSY 361
SR + + +ST+ V SKGNV KWL +
Sbjct: 1072 SRSSSELQKLSSTSSKVRSKGNVFWKWLGCF 1102
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,926,801
Number of Sequences: 28952
Number of extensions: 202960
Number of successful extensions: 705
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 683
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 705
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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