Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0231
(793 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 59 4e-09
At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 56 4e-08
At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 55 5e-08
At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 52 4e-07
At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 50 1e-06
At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma m... 36 0.031
At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma m... 36 0.031
At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma m... 35 0.071
At1g62420.1 68414.m07042 expressed protein contains Pfam profile... 29 4.7
At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma mem... 28 6.2
At1g12940.1 68414.m01503 high-affinity nitrate transporter, puta... 28 8.2
>At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to
profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis
thaliana]
Length = 134
Score = 58.8 bits (136), Expect = 4e-09
Identities = 29/63 (46%), Positives = 40/63 (63%), Gaps = 1/63 (1%)
Frame = +3
Query: 255 AGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLI 431
AG +Y+ + G + +IR K G G+ KT Q++V LYEEP+ P Q VVE+LG+YLI
Sbjct: 71 AGLKYMVIQGEPNAVIRGKKGAGGITIKKTGQSMVFGLYEEPVTPGQCNMVVERLGDYLI 130
Query: 432 TCG 440
G
Sbjct: 131 EQG 133
Score = 48.4 bits (110), Expect = 5e-06
Identities = 27/68 (39%), Positives = 36/68 (52%), Gaps = 7/68 (10%)
Frame = +1
Query: 67 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 225
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E+ I+ F+
Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANFPQFKPQEITDIMKDFDEP 60
Query: 226 SLLTSGGV 249
L G+
Sbjct: 61 GHLAPTGM 68
>At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to
profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis
thaliana]
Length = 134
Score = 55.6 bits (128), Expect = 4e-08
Identities = 27/63 (42%), Positives = 38/63 (60%), Gaps = 1/63 (1%)
Frame = +3
Query: 255 AGTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLI 431
AG +Y+ + G +IR K G G+ KT Q+ V +YEEP+ P Q VVE+LG+YL+
Sbjct: 71 AGAKYMVIQGEPGAVIRGKKGAGGITIKKTGQSCVFGIYEEPVTPGQCNMVVERLGDYLL 130
Query: 432 TCG 440
G
Sbjct: 131 EQG 133
Score = 48.8 bits (111), Expect = 4e-06
Identities = 28/70 (40%), Positives = 37/70 (52%), Gaps = 7/70 (10%)
Frame = +1
Query: 67 MSWQDYVDKQLMAS------RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENE 225
MSWQ YVD+ LM +T AAI GHDG+VWA+S F + E + I+ F+
Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANFPQFKGQEFSDIMKDFDEP 60
Query: 226 SLLTSGGVTM 255
L G+ M
Sbjct: 61 GHLAPTGLFM 70
>At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to
SP|Q9FE63 Profilin 5 {Arabidopsis thaliana}
Length = 168
Score = 55.2 bits (127), Expect = 5e-08
Identities = 28/62 (45%), Positives = 37/62 (59%), Gaps = 1/62 (1%)
Frame = +3
Query: 258 GTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLIT 434
G +Y+ + G + +IR K G GV KT A+V +Y+EP+ P Q VVE LGEYLI
Sbjct: 106 GNKYMVIQGEPNAVIRGKKGAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVENLGEYLIE 165
Query: 435 CG 440
G
Sbjct: 166 SG 167
Score = 48.4 bits (110), Expect = 5e-06
Identities = 27/65 (41%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +1
Query: 67 MSWQDYVDKQLM---ASRCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 234
MSWQ YVD LM A +T AAI G DG+VWA+S F ++ +E+ I F L
Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNFPQVKPEEIQGIKDDFTTPGTL 97
Query: 235 TSGGV 249
G+
Sbjct: 98 APTGL 102
>At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical
to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis
thaliana]; identical to cDNA profilin (PRF2) GI:9965570
Length = 131
Score = 52.0 bits (119), Expect = 4e-07
Identities = 27/62 (43%), Positives = 37/62 (59%), Gaps = 1/62 (1%)
Frame = +3
Query: 258 GTRYIYLSG-TDHIIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLIT 434
G +Y+ + G +IR K G GV KT QA+V +Y+EP+ Q VVE+LG+YLI
Sbjct: 69 GEKYMVVQGEAGAVIRGKKGPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVERLGDYLIE 128
Query: 435 CG 440
G
Sbjct: 129 SG 130
Score = 48.4 bits (110), Expect = 5e-06
Identities = 28/65 (43%), Positives = 35/65 (53%), Gaps = 4/65 (6%)
Frame = +1
Query: 67 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 234
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+A I FE L
Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFEEAGHL 60
Query: 235 TSGGV 249
G+
Sbjct: 61 APTGL 65
>At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen
Ara t 8 identical to profilin 1 (Allergen Ara t 8)
SP:Q42449 GI:1353770 from [Arabidopsis thaliana]
Length = 131
Score = 50.4 bits (115), Expect = 1e-06
Identities = 26/59 (44%), Positives = 35/59 (59%), Gaps = 1/59 (1%)
Frame = +3
Query: 258 GTRYIYLSGTDH-IIRAKLGKVGVHCMKTQQAVVISLYEEPIQPQQAASVVEKLGEYLI 431
G +Y+ + G +IR K G GV KT QA+V Y+EP+ Q VVE+LG+YLI
Sbjct: 69 GEKYMVIQGEQGAVIRGKKGPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVERLGDYLI 127
Score = 47.6 bits (108), Expect = 9e-06
Identities = 27/65 (41%), Positives = 34/65 (52%), Gaps = 4/65 (6%)
Frame = +1
Query: 67 MSWQDYVDKQLMAS---RCVTKAAIAGHDGNVWAKSEGF-EISKDEVAKIVAGFENESLL 234
MSWQ YVD LM +T AAI G DG+VWA+S F ++ E+ I FE L
Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60
Query: 235 TSGGV 249
G+
Sbjct: 61 APTGL 65
>At1g27770.2 68414.m03396 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 946
Score = 35.9 bits (79), Expect = 0.031
Identities = 19/51 (37%), Positives = 26/51 (50%)
Frame = +1
Query: 160 KSEGFEISKDEVAKIVAGFENESLLTSGGVTMRARGTSTSVAQTISSARSL 312
+ GFEI DE+ IV G + + L GG STS+A IS++ L
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGGTEGLTEKLSTSIASGISTSEDL 142
>At1g27770.1 68414.m03395 calcium-transporting ATPase 1, plasma
membrane-type / Ca(2+)-ATPase isoform 1 (ACA1) / plastid
envelope ATPase 1 (PEA1) identical to SP|Q37145
Calcium-transporting ATPase 1, plasma membrane-type (EC
3.6.3.8) (Ca(2+)-ATPase isoform 1) (Plastid envelope
ATPase 1) {Arabidopsis thaliana}; identical to cDNA
envelope Ca2+-ATPase (PEA1) chloroplast gene encoding
chloroplast protein GI:509809
Length = 1020
Score = 35.9 bits (79), Expect = 0.031
Identities = 19/51 (37%), Positives = 26/51 (50%)
Frame = +1
Query: 160 KSEGFEISKDEVAKIVAGFENESLLTSGGVTMRARGTSTSVAQTISSARSL 312
+ GFEI DE+ IV G + + L GG STS+A IS++ L
Sbjct: 92 RKAGFEICPDELGSIVEGHDLKKLKIHGGTEGLTEKLSTSIASGISTSEDL 142
>At2g41560.1 68415.m05136 calcium-transporting ATPase 4, plasma
membrane-type / Ca2+-ATPase, isoform 4 (ACA4) identical
to SP|O22218 Calcium-transporting ATPase 4, plasma
membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 4)
{Arabidopsis thaliana}
Length = 1030
Score = 34.7 bits (76), Expect = 0.071
Identities = 18/48 (37%), Positives = 27/48 (56%)
Frame = +1
Query: 160 KSEGFEISKDEVAKIVAGFENESLLTSGGVTMRARGTSTSVAQTISSA 303
K GF I DE+A +V + +SL GGV A+ S S+++ I S+
Sbjct: 89 KKAGFSIEADELASMVRKNDTKSLAQKGGVEELAKKVSVSLSEGIRSS 136
>At1g62420.1 68414.m07042 expressed protein contains Pfam profile
PF04720: Protein of unknown function (DUF506)
Length = 465
Score = 28.7 bits (61), Expect = 4.7
Identities = 12/49 (24%), Positives = 27/49 (55%)
Frame = +2
Query: 266 VHLPQWHRPYHPREAWQGRRALHEDTASCGHFSL*RTHPTSAGRICRGE 412
+H+P W R + + W G ++ T++ ++ + T+ T+A + C+ E
Sbjct: 213 IHVPPWRRNGYMQAKWFG---FYKRTSTTNNYEMVNTYDTTAFKGCKEE 258
>At2g22950.1 68415.m02725 calcium-transporting ATPase, plasma
membrane-type, putative / Ca2+-ATPase, putative (ACA7)
identical to SP|O64806 Potential calcium-transporting
ATPase 7, plasma membrane-type (EC 3.6.3.8)
(Ca(2+)-ATPase isoform 7) {Arabidopsis thaliana}; strong
similarity to SP|O81108 Calcium-transporting ATPase 2,
plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform
2) {Arabidopsis thaliana}
Length = 1015
Score = 28.3 bits (60), Expect = 6.2
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +1
Query: 160 KSEGFEISKDEVAKIVAGFENESLLTSGGV 249
K+ GF+I DE+ IV G + + L GGV
Sbjct: 90 KAAGFDICADELGSIVEGHDVKKLKFHGGV 119
>At1g12940.1 68414.m01503 high-affinity nitrate transporter,
putative similar to trans-membrane nitrate transporter
protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058,
high-affinity nitrate transporter ACH1 [Arabidopsis
thaliana] GI:3608362
Length = 502
Score = 27.9 bits (59), Expect = 8.2
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 142 DGNVWAKSEGFEISKDEVAKIVA-GFENESLLTSGGVTMRARGTSTSVAQTISS 300
DG+ WA + E KD+V K+++ G +N G +T A G V TI +
Sbjct: 233 DGDYWAMHKSGEREKDDVGKVISNGIKN----YRGWITALAYGYCFGVELTIDN 282
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,938,725
Number of Sequences: 28952
Number of extensions: 349182
Number of successful extensions: 767
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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