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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0228
         (713 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   111   1e-25
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   111   1e-25
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c...    26   4.7  
SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos...    26   6.1  
SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom...    26   6.1  

>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  111 bits (266), Expect = 1e-25
 Identities = 52/90 (57%), Positives = 55/90 (61%)
 Frame = +2

Query: 254 TSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXX 433
           TSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH         
Sbjct: 62  TSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKR 121

Query: 434 XXXXXXXXXXXXXXXXQARGHIIEKFSSFP 523
                            ARGH IE+    P
Sbjct: 122 YAIASAVAASGVPSLLLARGHRIEEIPEVP 151



 Score = 92.7 bits (220), Expect = 5e-20
 Identities = 39/69 (56%), Positives = 56/69 (81%)
 Frame = +1

Query: 505 KVLELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRK 684
           ++ E+PLVV D VQ   KTK+AV  L+ +KA+ D++KV  S++LRAGKGK+RNRR +QR+
Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRR 205

Query: 685 GPLIIFNKD 711
           GPL++FN+D
Sbjct: 206 GPLVVFNED 214



 Score = 79.0 bits (186), Expect = 6e-16
 Identities = 36/62 (58%), Positives = 46/62 (74%)
 Frame = +3

Query: 69  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 248
           ++ ARP VS+Y+ K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++AG
Sbjct: 1   MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59

Query: 249 HQ 254
           HQ
Sbjct: 60  HQ 61


>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  111 bits (266), Expect = 1e-25
 Identities = 52/90 (57%), Positives = 55/90 (61%)
 Frame = +2

Query: 254 TSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXX 433
           TSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH         
Sbjct: 62  TSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKR 121

Query: 434 XXXXXXXXXXXXXXXXQARGHIIEKFSSFP 523
                            ARGH IE+    P
Sbjct: 122 YAISSAVAASGVPSLLLARGHRIEEIPEVP 151



 Score = 92.3 bits (219), Expect = 6e-20
 Identities = 39/69 (56%), Positives = 56/69 (81%)
 Frame = +1

Query: 505 KVLELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRK 684
           ++ E+PLVV D VQ   KTK+AV  L+ +KA+ D++KV  S++LRAGKGK+RNRR +QR+
Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRR 205

Query: 685 GPLIIFNKD 711
           GPL++FN+D
Sbjct: 206 GPLVVFNED 214



 Score = 79.8 bits (188), Expect = 4e-16
 Identities = 37/62 (59%), Positives = 46/62 (74%)
 Frame = +3

Query: 69  LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 248
           ++ ARP VS+YS K  +V      LPFVFKAPIRPDLV  VH +++KN RQPY VS++AG
Sbjct: 1   MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59

Query: 249 HQ 254
           HQ
Sbjct: 60  HQ 61


>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 606

 Score = 26.2 bits (55), Expect = 4.7
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = -2

Query: 130 APCTVSLFSEYTDTKGRATDRLISLL 53
           A C V ++SEYT   G ++  L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515


>SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 532

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/37 (35%), Positives = 19/37 (51%)
 Frame = +2

Query: 68  SIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGP 178
           SI SP+ SVG   +  D +  S + P+ I G  +  P
Sbjct: 312 SITSPSSSVGQIPQPTDHSALSPSKPMSISGTESPNP 348


>SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 355

 Score = 25.8 bits (54), Expect = 6.1
 Identities = 13/32 (40%), Positives = 16/32 (50%)
 Frame = +2

Query: 143 PVRIQGAHTSGPGQ*CSRFYVQELEAALLREQ 238
           PV +QG HT G      +F  Q L A +L  Q
Sbjct: 29  PVDVQGCHTLGERNDPKKFRFQTLVALMLSSQ 60


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,758,934
Number of Sequences: 5004
Number of extensions: 54026
Number of successful extensions: 175
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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