BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0228 (713 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 111 1e-25 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 111 1e-25 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 4.7 SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 26 6.1 SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pom... 26 6.1 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 111 bits (266), Expect = 1e-25 Identities = 52/90 (57%), Positives = 55/90 (61%) Frame = +2 Query: 254 TSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXX 433 TSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 62 TSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKR 121 Query: 434 XXXXXXXXXXXXXXXXQARGHIIEKFSSFP 523 ARGH IE+ P Sbjct: 122 YAIASAVAASGVPSLLLARGHRIEEIPEVP 151 Score = 92.7 bits (220), Expect = 5e-20 Identities = 39/69 (56%), Positives = 56/69 (81%) Frame = +1 Query: 505 KVLELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRK 684 ++ E+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+ Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRR 205 Query: 685 GPLIIFNKD 711 GPL++FN+D Sbjct: 206 GPLVVFNED 214 Score = 79.0 bits (186), Expect = 6e-16 Identities = 36/62 (58%), Positives = 46/62 (74%) Frame = +3 Query: 69 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 248 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59 Query: 249 HQ 254 HQ Sbjct: 60 HQ 61 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 111 bits (266), Expect = 1e-25 Identities = 52/90 (57%), Positives = 55/90 (61%) Frame = +2 Query: 254 TSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXX 433 TSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 62 TSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKR 121 Query: 434 XXXXXXXXXXXXXXXXQARGHIIEKFSSFP 523 ARGH IE+ P Sbjct: 122 YAISSAVAASGVPSLLLARGHRIEEIPEVP 151 Score = 92.3 bits (219), Expect = 6e-20 Identities = 39/69 (56%), Positives = 56/69 (81%) Frame = +1 Query: 505 KVLELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRK 684 ++ E+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR+ Sbjct: 146 EIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRR 205 Query: 685 GPLIIFNKD 711 GPL++FN+D Sbjct: 206 GPLVVFNED 214 Score = 79.8 bits (188), Expect = 4e-16 Identities = 37/62 (59%), Positives = 46/62 (74%) Frame = +3 Query: 69 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAG 248 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AG Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAG 59 Query: 249 HQ 254 HQ Sbjct: 60 HQ 61 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 4.7 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -2 Query: 130 APCTVSLFSEYTDTKGRATDRLISLL 53 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 532 Score = 25.8 bits (54), Expect = 6.1 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +2 Query: 68 SIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGP 178 SI SP+ SVG + D + S + P+ I G + P Sbjct: 312 SITSPSSSVGQIPQPTDHSALSPSKPMSISGTESPNP 348 >SPAC30D11.07 |nth1||DNA endonuclease III|Schizosaccharomyces pombe|chr 1|||Manual Length = 355 Score = 25.8 bits (54), Expect = 6.1 Identities = 13/32 (40%), Positives = 16/32 (50%) Frame = +2 Query: 143 PVRIQGAHTSGPGQ*CSRFYVQELEAALLREQ 238 PV +QG HT G +F Q L A +L Q Sbjct: 29 PVDVQGCHTLGERNDPKKFRFQTLVALMLSSQ 60 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,758,934 Number of Sequences: 5004 Number of extensions: 54026 Number of successful extensions: 175 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 173 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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