Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0228
(713 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 113 1e-25
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 113 1e-25
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 31 0.57
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 31 0.76
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.3
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.1
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.1
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 5.3
At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1 GI... 27 9.3
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 113 bits (271), Expect = 1e-25
Identities = 53/90 (58%), Positives = 55/90 (61%)
Frame = +2
Query: 254 TSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXX 433
TSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 68 TSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKR 127
Query: 434 XXXXXXXXXXXXXXXXQARGHIIEKFSSFP 523
ARGH IE P
Sbjct: 128 HAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 69.7 bits (163), Expect = 2e-12
Identities = 32/68 (47%), Positives = 45/68 (66%)
Frame = +1
Query: 508 VLELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 687
V E+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 153 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 212
Query: 688 PLIIFNKD 711
PL++F +
Sbjct: 213 PLVVFGTE 220
Score = 67.7 bits (158), Expect = 7e-12
Identities = 34/63 (53%), Positives = 41/63 (65%), Gaps = 2/63 (3%)
Frame = +3
Query: 72 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 245
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
Query: 246 GHQ 254
GHQ
Sbjct: 65 GHQ 67
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 113 bits (271), Expect = 1e-25
Identities = 53/90 (58%), Positives = 55/90 (61%)
Frame = +2
Query: 254 TSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXXXX 433
TSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 67 TSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNMKR 126
Query: 434 XXXXXXXXXXXXXXXXQARGHIIEKFSSFP 523
ARGH IE P
Sbjct: 127 HAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 69.7 bits (163), Expect = 2e-12
Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 2/66 (3%)
Frame = +3
Query: 63 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 236
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 237 KEAGHQ 254
K+AGHQ
Sbjct: 61 KKAGHQ 66
Score = 68.5 bits (160), Expect = 4e-12
Identities = 31/68 (45%), Positives = 45/68 (66%)
Frame = +1
Query: 508 VLELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQRKG 687
V E+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I RKG
Sbjct: 152 VPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISRKG 211
Query: 688 PLIIFNKD 711
PL+++ +
Sbjct: 212 PLVVYGTE 219
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 31.5 bits (68), Expect = 0.57
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
Frame = +2
Query: 212 LEAALLREQGGWSPTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 382
+ AL+R + G PT AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMGAKPT-AEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 383 TKPW 394
W
Sbjct: 135 ISKW 138
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 31.1 bits (67), Expect = 0.76
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -1
Query: 365 HHDTCYRRHPDRTYGYHHHGHAEF 294
HH Y H YGY +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +3
Query: 87 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 233
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 29.1 bits (62), Expect = 3.1
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 361 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 459
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 29.1 bits (62), Expect = 3.1
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 361 WWTYVRPHEALAALAPSRQPPTAESGLGSSRCC 459
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 5.3
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 138 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 233
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 9.3
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 138 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 233
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At3g50110.1 68416.m05478 phosphatase-related similar to PTEN1
GI:5566292 from [Drosophila melanogaster]; contains
prosite evidence: PS00383: Tyrosine specific protein
phosphatases active site
Length = 632
Score = 27.5 bits (58), Expect = 9.3
Identities = 16/45 (35%), Positives = 22/45 (48%)
Frame = -2
Query: 523 REARELFNNVSSSLNERWDASSSNGCCQGRSPLSEVDATVPAPPG 389
REA E + +S+ + D + G PLS+ D VP PPG
Sbjct: 42 REAHEDSISTEASV-AKVDDTQMPASSTGSEPLSKTDDIVPCPPG 85
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,124,298
Number of Sequences: 28952
Number of extensions: 330925
Number of successful extensions: 1195
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1115
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1187
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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