BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0227 (465 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 29 0.46 SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 28 0.81 SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 27 1.9 SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 25 4.3 SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces p... 25 7.5 SPCC1259.09c |||pyruvate dehydrogenase protein x component|Schiz... 24 9.9 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 24 9.9 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 24 9.9 SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 24 9.9 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 28.7 bits (61), Expect = 0.46 Identities = 21/56 (37%), Positives = 31/56 (55%), Gaps = 4/56 (7%) Frame = +3 Query: 72 AFEEKEEELRDLYADLKHKLNIADI*IMDLKGSLVDTNVR---IYPTNGF-LEYIK 227 +F+EK EL +LK KLN A+ +L S ++NV+ + P NG LE +K Sbjct: 584 SFQEKINELNSQIDELKLKLNEANKKYQELAISFENSNVKTQSVEPDNGLSLEALK 639 >SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 27.9 bits (59), Expect = 0.81 Identities = 11/30 (36%), Positives = 18/30 (60%) Frame = +2 Query: 278 SDYETKCDVKDFDYFAKTVNLTNEDIELLR 367 SDYE +C V+D + T+ +T D +L+ Sbjct: 406 SDYEKECKVQDIGLYEDTILITLSDGRILK 435 >SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1639 Score = 26.6 bits (56), Expect = 1.9 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +2 Query: 254 NILKLKNGSDYETKCDVKDFDYFAKTVNLTNEDIE 358 N +K N + T+C V+ DY TV L E I+ Sbjct: 1120 NAIKFTNVGEVFTRCSVEKIDYSTNTVVLKWECID 1154 >SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 900 Score = 25.4 bits (53), Expect = 4.3 Identities = 13/56 (23%), Positives = 28/56 (50%) Frame = +2 Query: 287 ETKCDVKDFDYFAKTVNLTNEDIELLRTIKKTISRGSAVKINNTEIEPEFVEHDKI 454 E + D + A N+ ED+ L +T+ +TI++ K + + F+E+ ++ Sbjct: 711 ELALEADDCELAATIANIPEEDVVLKKTLWQTIAKYMFSKKSGIKETLRFLENSEV 766 >SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 24.6 bits (51), Expect = 7.5 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +3 Query: 63 IDNAFEEKEEELRDLYADLKHKL 131 ID F E+ E +R+ YAD H L Sbjct: 85 IDFEFSEELEPIRNAYADSLHNL 107 >SPCC1259.09c |||pyruvate dehydrogenase protein x component|Schizosaccharomyces pombe|chr 3|||Manual Length = 456 Score = 24.2 bits (50), Expect = 9.9 Identities = 14/37 (37%), Positives = 20/37 (54%), Gaps = 4/37 (10%) Frame = +2 Query: 341 TNEDIELLRTIKKTISRGSAVK----INNTEIEPEFV 439 T ED+ L +IK IS+ A+K + + PEFV Sbjct: 416 TTEDLTLTLSIKDNISKSRAIKFVDCLKSNFENPEFV 452 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 24.2 bits (50), Expect = 9.9 Identities = 10/21 (47%), Positives = 12/21 (57%) Frame = -3 Query: 460 CVYFIMFNKLRLYFCVIYFHS 398 CVY I F L YF YF++ Sbjct: 1129 CVYDISFGSLTFYFQKSYFNA 1149 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 24.2 bits (50), Expect = 9.9 Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 1/46 (2%) Frame = +2 Query: 227 RNQR*QSTRNILKLKNGSDY-ETKCDVKDFDYFAKTVNLTNEDIEL 361 RNQ Q RNI K SDY +T+ D + + AK +I L Sbjct: 396 RNQLLQINRNINSTKENSDYLKTEYDEMENELKAKLSRKKEIEISL 441 >SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosaccharomyces pombe|chr 1|||Manual Length = 988 Score = 24.2 bits (50), Expect = 9.9 Identities = 11/35 (31%), Positives = 20/35 (57%) Frame = +2 Query: 260 LKLKNGSDYETKCDVKDFDYFAKTVNLTNEDIELL 364 LK+++GS+ E++ D ++ + TN D LL Sbjct: 62 LKVESGSERESQSDKDSSKKYSDSAKSTNSDDHLL 96 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,607,773 Number of Sequences: 5004 Number of extensions: 27456 Number of successful extensions: 82 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 80 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 82 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 176367270 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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