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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0225
         (822 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,...    76   2e-14
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E...    70   2e-12
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-...    52   5e-07
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ...    51   8e-07
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ...    51   8e-07
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ...    51   8e-07
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ...    51   8e-07
At2g32000.1 68415.m03910 DNA topoisomerase family protein simila...    28   6.5  

>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
           putative similar to EF-1-alpha-related GTP-binding
           protein gi|1009232|gb|AAA79032
          Length = 532

 Score = 76.2 bits (179), Expect = 2e-14
 Identities = 35/83 (42%), Positives = 49/83 (59%)
 Frame = +3

Query: 3   VMHIHCAAEEITVKALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFA 182
           ++HIH   EE  +  L   +D KT +  K +  FVK     + RI+    IC+E F  F 
Sbjct: 444 ILHIHAVVEECEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFP 503

Query: 183 QMGRFTLRDENKTIAIGKVLKVI 251
           Q+GRFTLR E KTIA+GKV +++
Sbjct: 504 QLGRFTLRTEGKTIAVGKVTELL 526


>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
           EF-1-alpha, putative contains similarity to
           SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
           [Aeropyrum pernix]
          Length = 667

 Score = 69.7 bits (163), Expect = 2e-12
 Identities = 30/84 (35%), Positives = 50/84 (59%)
 Frame = +3

Query: 9   HIHCAAEEITVKALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQM 188
           H+H A E  TV  L+ ++D KTG+ +K  PR +   Q A++ +     +C+E F +   +
Sbjct: 584 HVHHAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRAL 643

Query: 189 GRFTLRDENKTIAIGKVLKVIEKS 260
           GR  LR   +T+A+GKV ++I+ S
Sbjct: 644 GRVFLRSSGRTVAMGKVTRIIQDS 667


>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
           domain-containing protein similar to SP|P13905
           Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
           thaliana}; contains Pfam profile PF03143: Elongation
           factor Tu C-terminal domain
          Length = 104

 Score = 52.0 bits (119), Expect = 5e-07
 Identities = 22/84 (26%), Positives = 46/84 (54%), Gaps = 2/84 (2%)
 Frame = +3

Query: 12  IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185
           + C    I VK   ++  +D +TG + +  P+F+K  + AI+ +     + +E +  +  
Sbjct: 15  LDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPP 74

Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257
           +GRF +RD  +T+ +G +  V++K
Sbjct: 75  LGRFAIRDMRQTVGVGVIKSVVKK 98


>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
           identical to SWISS-PROT:P13905 elongation factor 1-alpha
           (EF-1-alpha) [Arabidopsis thaliana]
          Length = 449

 Score = 51.2 bits (117), Expect = 8e-07
 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
 Frame = +3

Query: 12  IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185
           + C    I VK   ++  +D+++G++ +  P+F+K     ++++     + +E F ++  
Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408

Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257
           +GRF +RD  +T+A+G V+K ++K
Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431


>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
           identical to GB:CAA34456 from [Arabidopsis thaliana]
           (Plant Mol. Biol. 14 (1), 107-110 (1990))
          Length = 449

 Score = 51.2 bits (117), Expect = 8e-07
 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
 Frame = +3

Query: 12  IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185
           + C    I VK   ++  +D+++G++ +  P+F+K     ++++     + +E F ++  
Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408

Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257
           +GRF +RD  +T+A+G V+K ++K
Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431


>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
           identical to GB:CAA34456 from [Arabidopsis thaliana]
           (Plant Mol. Biol. 14 (1), 107-110 (1990))
          Length = 449

 Score = 51.2 bits (117), Expect = 8e-07
 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
 Frame = +3

Query: 12  IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185
           + C    I VK   ++  +D+++G++ +  P+F+K     ++++     + +E F ++  
Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408

Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257
           +GRF +RD  +T+A+G V+K ++K
Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431


>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
           identical to GB:CAA34456 from [Arabidopsis thaliana]
           (Plant Mol. Biol. 14 (1), 107-110 (1990))
          Length = 449

 Score = 51.2 bits (117), Expect = 8e-07
 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
 Frame = +3

Query: 12  IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185
           + C    I VK   ++  +D+++G++ +  P+F+K     ++++     + +E F ++  
Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408

Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257
           +GRF +RD  +T+A+G V+K ++K
Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431


>At2g32000.1 68415.m03910 DNA topoisomerase family protein similar
           to DNA topoisomerase III beta-1 (EC 5.99.1.2)(SP:Q9Z321)
           {Mus musculus}
          Length = 865

 Score = 28.3 bits (60), Expect = 6.5
 Identities = 10/42 (23%), Positives = 23/42 (54%)
 Frame = +3

Query: 15  HCAAEEITVKALICLVDKKTGEKSKTRPRFVKQDQVAIMRIE 140
           HC  + +T K    ++     ++ K  P F+K +++ ++R+E
Sbjct: 436 HCTGQRVTEKGFTAIMPWSAVDEKKL-PSFLKGERIEVLRVE 476


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,326,222
Number of Sequences: 28952
Number of extensions: 290571
Number of successful extensions: 584
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 572
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 583
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1882599200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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