BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0225 (822 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 76 2e-14 At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 70 2e-12 At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 52 5e-07 At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 51 8e-07 At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 51 8e-07 At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 51 8e-07 At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 51 8e-07 At2g32000.1 68415.m03910 DNA topoisomerase family protein simila... 28 6.5 >At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein, putative similar to EF-1-alpha-related GTP-binding protein gi|1009232|gb|AAA79032 Length = 532 Score = 76.2 bits (179), Expect = 2e-14 Identities = 35/83 (42%), Positives = 49/83 (59%) Frame = +3 Query: 3 VMHIHCAAEEITVKALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFA 182 ++HIH EE + L +D KT + K + FVK + RI+ IC+E F F Sbjct: 444 ILHIHAVVEECEIIELKSQIDLKTRKPMKKKVLFVKNGAAVVCRIQVTNSICIEKFSDFP 503 Query: 183 QMGRFTLRDENKTIAIGKVLKVI 251 Q+GRFTLR E KTIA+GKV +++ Sbjct: 504 QLGRFTLRTEGKTIAVGKVTELL 526 >At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / EF-1-alpha, putative contains similarity to SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha) [Aeropyrum pernix] Length = 667 Score = 69.7 bits (163), Expect = 2e-12 Identities = 30/84 (35%), Positives = 50/84 (59%) Frame = +3 Query: 9 HIHCAAEEITVKALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQM 188 H+H A E TV L+ ++D KTG+ +K PR + Q A++ + +C+E F + + Sbjct: 584 HVHHAKEAATVVKLVAMLDPKTGQPTKKSPRCLTAKQSAMLEVSLQNPVCVETFSESRAL 643 Query: 189 GRFTLRDENKTIAIGKVLKVIEKS 260 GR LR +T+A+GKV ++I+ S Sbjct: 644 GRVFLRSSGRTVAMGKVTRIIQDS 667 >At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-containing protein similar to SP|P13905 Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis thaliana}; contains Pfam profile PF03143: Elongation factor Tu C-terminal domain Length = 104 Score = 52.0 bits (119), Expect = 5e-07 Identities = 22/84 (26%), Positives = 46/84 (54%), Gaps = 2/84 (2%) Frame = +3 Query: 12 IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185 + C I VK ++ +D +TG + + P+F+K + AI+ + + +E + + Sbjct: 15 LDCHTSHIAVKFSEILTKIDWRTGHEIEKEPKFLKNSEAAIINMTPTKPMVVEAYSAYPP 74 Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257 +GRF +RD +T+ +G + V++K Sbjct: 75 LGRFAIRDMRQTVGVGVIKSVVKK 98 >At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha identical to SWISS-PROT:P13905 elongation factor 1-alpha (EF-1-alpha) [Arabidopsis thaliana] Length = 449 Score = 51.2 bits (117), Expect = 8e-07 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%) Frame = +3 Query: 12 IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185 + C I VK ++ +D+++G++ + P+F+K ++++ + +E F ++ Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408 Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257 +GRF +RD +T+A+G V+K ++K Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431 >At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 51.2 bits (117), Expect = 8e-07 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%) Frame = +3 Query: 12 IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185 + C I VK ++ +D+++G++ + P+F+K ++++ + +E F ++ Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408 Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257 +GRF +RD +T+A+G V+K ++K Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431 >At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 51.2 bits (117), Expect = 8e-07 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%) Frame = +3 Query: 12 IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185 + C I VK ++ +D+++G++ + P+F+K ++++ + +E F ++ Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408 Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257 +GRF +RD +T+A+G V+K ++K Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431 >At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha identical to GB:CAA34456 from [Arabidopsis thaliana] (Plant Mol. Biol. 14 (1), 107-110 (1990)) Length = 449 Score = 51.2 bits (117), Expect = 8e-07 Identities = 22/84 (26%), Positives = 50/84 (59%), Gaps = 2/84 (2%) Frame = +3 Query: 12 IHCAAEEITVK--ALICLVDKKTGEKSKTRPRFVKQDQVAIMRIECAGVICLEPFKKFAQ 185 + C I VK ++ +D+++G++ + P+F+K ++++ + +E F ++ Sbjct: 349 LDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEYPP 408 Query: 186 MGRFTLRDENKTIAIGKVLKVIEK 257 +GRF +RD +T+A+G V+K ++K Sbjct: 409 LGRFAVRDMRQTVAVG-VIKSVDK 431 >At2g32000.1 68415.m03910 DNA topoisomerase family protein similar to DNA topoisomerase III beta-1 (EC 5.99.1.2)(SP:Q9Z321) {Mus musculus} Length = 865 Score = 28.3 bits (60), Expect = 6.5 Identities = 10/42 (23%), Positives = 23/42 (54%) Frame = +3 Query: 15 HCAAEEITVKALICLVDKKTGEKSKTRPRFVKQDQVAIMRIE 140 HC + +T K ++ ++ K P F+K +++ ++R+E Sbjct: 436 HCTGQRVTEKGFTAIMPWSAVDEKKL-PSFLKGERIEVLRVE 476 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,326,222 Number of Sequences: 28952 Number of extensions: 290571 Number of successful extensions: 584 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 572 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 583 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1882599200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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