Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0223
(776 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces po... 134 1e-32
SPAC732.01 |vma11||V-type ATPase proteolipid subunit|Schizosacch... 118 1e-27
SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces... 58 1e-09
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.24
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.3
SPAC212.04c |||S. pombe specific DUF999 family protein 1|Schizos... 28 1.7
SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|c... 27 3.0
SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 9.2
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 9.2
SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces pombe... 25 9.2
>SPAC1B3.14 |vma3||V-type ATPase subunit c|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 161
Score = 134 bits (325), Expect = 1e-32
Identities = 70/87 (80%), Positives = 77/87 (88%)
Frame = +2
Query: 254 AGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVR 433
AGIIAIYGLVV+VLI+G L++ + LY GFI LGAGL+VG +GLAAGFAIGIVGDAGVR
Sbjct: 61 AGIIAIYGLVVSVLISGNLKQILS--LYSGFIQLGAGLSVGLAGLAAGFAIGIVGDAGVR 118
Query: 434 GTAQQPRLFVGMILILIFAEVLGLYDL 514
GTAQQPRLFV MILILIFAEVLGLY L
Sbjct: 119 GTAQQPRLFVAMILILIFAEVLGLYGL 145
Score = 85.8 bits (203), Expect = 6e-18
Identities = 36/54 (66%), Positives = 47/54 (87%)
Frame = +3
Query: 93 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVM 254
P+Y PFFGVMG +AI+F++ GAAYGTAK+G GI+AM V+RP+LI+K+ IPVVM
Sbjct: 7 PVYAPFFGVMGCTAAIVFASFGAAYGTAKAGVGISAMGVLRPDLIVKNTIPVVM 60
>SPAC732.01 |vma11||V-type ATPase proteolipid
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 162
Score = 118 bits (283), Expect = 1e-27
Identities = 55/87 (63%), Positives = 70/87 (80%)
Frame = +2
Query: 254 AGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVR 433
+GII +YGLV++VLIAG + +Y L+ GFIHL AGLAVG +G+AAG+AIG+VGD GV+
Sbjct: 61 SGIIGVYGLVMSVLIAGDMSPDNDYSLFSGFIHLSAGLAVGLTGVAAGYAIGVVGDRGVQ 120
Query: 434 GTAQQPRLFVGMILILIFAEVLGLYDL 514
+Q R+FV M+LILIFAEVLGLY L
Sbjct: 121 SFMRQDRIFVSMVLILIFAEVLGLYGL 147
Score = 74.9 bits (176), Expect = 1e-14
Identities = 32/54 (59%), Positives = 41/54 (75%)
Frame = +3
Query: 93 PIYGPFFGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVVM 254
PIY FFG G ++++FS LGA YGTA +G GIAA+ RPE++MKS+IPVVM
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVM 60
Score = 29.5 bits (63), Expect = 0.56
Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Frame = +2
Query: 329 PLYKGFIHL-GAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGL 505
P+Y F G ++ FS L AG+ + G A +P + + ++ ++ + ++G+
Sbjct: 7 PIYSSFFGFAGVCASMVFSCLGAGYGTALAGRGIAAVGAFRPEIVMKSLIPVVMSGIIGV 66
Query: 506 YDL 514
Y L
Sbjct: 67 YGL 69
>SPAC2C4.13 |vma16||V-type ATPase subunit c''|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 199
Score = 58.4 bits (135), Expect = 1e-09
Identities = 30/91 (32%), Positives = 48/91 (52%), Gaps = 6/91 (6%)
Frame = +2
Query: 260 IIAIYGLVVAVLIAGALQE--PANY----PLYKGFIHLGAGLAVGFSGLAAGFAIGIVGD 421
++AIY L++A++ + + + PA + Y GF G+ VG L G +GI G
Sbjct: 95 VVAIYSLIIAIVFSAKINDINPAGFYTKSHYYTGFALFWGGITVGLCNLICGVCVGITGS 154
Query: 422 AGVRGTAQQPRLFVGMILILIFAEVLGLYDL 514
+ AQ LFV ++++ IF VLGL+ L
Sbjct: 155 SAALADAQDASLFVKVLVVEIFGSVLGLFGL 185
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/47 (34%), Positives = 28/47 (59%)
Frame = +3
Query: 111 FGVMGAASAIIFSALGAAYGTAKSGTGIAAMSVMRPELIMKSIIPVV 251
+G++G AS + F +GAA+G GT I +V P + K++I ++
Sbjct: 45 WGLLGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISII 91
Score = 34.3 bits (75), Expect = 0.020
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +2
Query: 353 LGAGLAVGFSGLAAGFAIGIVGDAGVRGTAQQPRLFVGMILILIFAEVLGLYDL 514
LG V F + A + I I G + + G + PR+ ++ +IF EV+ +Y L
Sbjct: 48 LGIASCVAFGIIGAAWGIFICGTSILGGAVKAPRIKTKNLISIIFCEVVAIYSL 101
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.24
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -3
Query: 441 AVPRTPASPTMPMAKPAARPENPTAKPAPKWMNP 340
+VP P++P MP P+A P A AP NP
Sbjct: 1713 SVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.3
Identities = 26/65 (40%), Positives = 30/65 (46%), Gaps = 2/65 (3%)
Frame = -3
Query: 441 AVPRTPAS--PTMPMAKPAARPENPTAKPAPKWMNPL*RG*LAGSWRAPAIRTATTRP*M 268
A P P S P++PM PAA P P+A AP PL AG AP + A P
Sbjct: 427 APPSLPPSAPPSLPMGAPAAPPLPPSAPIAP----PL----PAGMPAAPPLPPAAPAPPP 478
Query: 267 AIIPA 253
A PA
Sbjct: 479 APAPA 483
>SPAC212.04c |||S. pombe specific DUF999 family protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 288
Score = 27.9 bits (59), Expect = 1.7
Identities = 19/62 (30%), Positives = 27/62 (43%)
Frame = +2
Query: 254 AGIIAIYGLVVAVLIAGALQEPANYPLYKGFIHLGAGLAVGFSGLAAGFAIGIVGDAGVR 433
AG+IA + +++ IAG + G ++ G L LA GF I GD V
Sbjct: 199 AGVIAAFSVIITATIAGVIAAMV------GILYFGHWLVYKILILAFGFKIVTSGDVCVS 252
Query: 434 GT 439
T
Sbjct: 253 NT 254
>SPAC607.08c |||DUF726 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 579
Score = 27.1 bits (57), Expect = 3.0
Identities = 21/55 (38%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Frame = +2
Query: 365 LAVGFSGLAAGFAIGIVGD-AGVRGTAQQPRLFVGMIL-ILIFAEVLGLYDLSSP 523
+A+G +GLA G IG+ G A A LF G+ L +I A LG S+P
Sbjct: 173 IAMGLAGLAGGALIGLTGGLAAPFVAAGLGTLFAGLGLGTMIGATYLGTLITSAP 227
>SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 25.4 bits (53), Expect = 9.2
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = -3
Query: 51 TGPKNCDDYLHTL 13
TGP N DDY+H +
Sbjct: 369 TGPSNTDDYIHRI 381
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = -3
Query: 441 AVPRTPASPTMPMA----KPAARPENPTAKPAPK 352
+VP+ PA+P +P A +P A P P AP+
Sbjct: 513 SVPQPPAAPVVPEAPSVHQPPAAPVAPEVPSAPQ 546
>SPAC4D7.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 281
Score = 25.4 bits (53), Expect = 9.2
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = -2
Query: 523 WRR*VVKTQYFSENKNKNHSDE*PRLLSSTT 431
W+ V K+QY +EN N N + P L S+TT
Sbjct: 241 WKDLVWKSQYATENANTNSINNSP-LSSNTT 270
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,473,221
Number of Sequences: 5004
Number of extensions: 75361
Number of successful extensions: 221
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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