BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0221 (764 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 89 2e-18 At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 86 2e-17 At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 83 1e-16 At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger) fa... 29 3.4 At5g18490.1 68418.m02181 expressed protein 29 3.4 At3g54980.1 68416.m06100 pentatricopeptide (PPR) repeat-containi... 28 7.8 At1g34060.1 68414.m04222 alliinase family protein contains Pfam ... 28 7.8 >At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase precursor SP:P43077 from [Candida albicans] Length = 580 Score = 89.4 bits (212), Expect = 2e-18 Identities = 35/78 (44%), Positives = 50/78 (64%) Frame = +2 Query: 2 KMNVFHWHIVDDQSFPYQSERFPDLSRLGAYHETLIYTKENIQTVIDHARNRGIRVIPEF 181 K+NVFHWHI D QSFP P L+ G+ ++YT E++ ++ + G+RV+PE Sbjct: 201 KLNVFHWHITDSQSFPLVLPSEPSLAAKGSLGPDMVYTPEDVSKIVQYGFEHGVRVLPEI 260 Query: 182 DVPGHTRSWGVAKPDLLT 235 D PGHT SWG A P+++T Sbjct: 261 DTPGHTGSWGEAYPEIVT 278 Score = 51.2 bits (117), Expect = 7e-07 Identities = 21/81 (25%), Positives = 45/81 (55%), Gaps = 1/81 (1%) Frame = +1 Query: 265 GLGPMNPIKDSTYTFLQELFHEVQALFPDRYIHIGGDEVDLDCWESNPEFQRYIQEHNLT 444 G G +NP+ TY ++ + ++ FP+ + H GGDEV CW+++P ++ Sbjct: 300 GTGQLNPLSPKTYEVVKNVIQDIVNQFPESFFHGGGDEVIPGCWKTDPAINSFLSSGG-- 357 Query: 445 SVADFHALFMRNTIP-LLSEN 504 +++ ++ +T+P ++S+N Sbjct: 358 TLSQLLEKYINSTLPYIVSQN 378 >At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase A SP:P13723 from [Dictyostelium discoideum] Length = 541 Score = 86.2 bits (204), Expect = 2e-17 Identities = 38/76 (50%), Positives = 50/76 (65%) Frame = +2 Query: 2 KMNVFHWHIVDDQSFPYQSERFPDLSRLGAYHETLIYTKENIQTVIDHARNRGIRVIPEF 181 K+NV HWHIVD+QSFP ++ +P+L + GAY YT E+ ++ A+ RGI V+ E Sbjct: 215 KLNVLHWHIVDEQSFPLETPTYPNLWK-GAYSRWERYTVEDASEIVRFAKMRGINVMAEV 273 Query: 182 DVPGHTRSWGVAKPDL 229 DVPGH SWG PDL Sbjct: 274 DVPGHAESWGTGYPDL 289 Score = 51.2 bits (117), Expect = 7e-07 Identities = 20/77 (25%), Positives = 42/77 (54%) Frame = +1 Query: 274 PMNPIKDSTYTFLQELFHEVQALFPDRYIHIGGDEVDLDCWESNPEFQRYIQEHNLTSVA 453 P++ K+ T+ + + +++ +FP H+GGDEV+ DCW++ + ++Q N T+ Sbjct: 298 PLDVTKNFTFDVISGILADMRKIFPFELFHLGGDEVNTDCWKNTTHVKEWLQGRNFTTKD 357 Query: 454 DFHALFMRNTIPLLSEN 504 + +R +S+N Sbjct: 358 AYKYFVLRAQQIAISKN 374 Score = 30.3 bits (65), Expect = 1.5 Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 1/69 (1%) Frame = +2 Query: 560 TIVQVWKENEAPEMLNILRASHQLIYSTG-WYLDHLNTGGDWTEFFNKDPRDLVNGLSKD 736 T++Q W ++ + + + + + G WYLDHL+ W E +N +P +NG+ Sbjct: 395 TVIQNWLVSDICQKA-VAKGFRCIFSNQGYWYLDHLDV--PWEEVYNTEP---LNGIEDP 448 Query: 737 INVDNIVGG 763 ++GG Sbjct: 449 SLQKLVIGG 457 >At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein contains Pfam PF00728: Glycosyl hydrolase family 20, catalytic domain; contains Pfam PF02838: Glycosyl hydrolase family 20, domain 2; similar to Beta-hexosaminidase beta chain precursor (EC 3.2.1.52) (N-acetyl-beta- glucosaminidase) (Beta-N-acetylhexosaminidase) (Hexosaminidase B) (Swiss-Prot:P07686) [Homo sapiens] Length = 535 Score = 83.4 bits (197), Expect = 1e-16 Identities = 38/76 (50%), Positives = 48/76 (63%) Frame = +2 Query: 2 KMNVFHWHIVDDQSFPYQSERFPDLSRLGAYHETLIYTKENIQTVIDHARNRGIRVIPEF 181 K+NV HWHIVD QSFP + +P L GAY + YT E+ ++++AR RGI V+ E Sbjct: 212 KLNVLHWHIVDTQSFPLEIPSYPKLWN-GAYSSSQRYTFEDAAEIVNYARRRGIHVLAEI 270 Query: 182 DVPGHTRSWGVAKPDL 229 DVPGH SWG P L Sbjct: 271 DVPGHALSWGKGYPAL 286 Score = 49.2 bits (112), Expect = 3e-06 Identities = 15/68 (22%), Positives = 37/68 (54%) Frame = +1 Query: 274 PMNPIKDSTYTFLQELFHEVQALFPDRYIHIGGDEVDLDCWESNPEFQRYIQEHNLTSVA 453 P++ D T+ + + + +F +++H+GGDEV+ CW + P +++++H ++ Sbjct: 295 PLDVSSDFTFKVIDGILSDFSKIFKFKFVHLGGDEVNTTCWSATPRIAQWLKKHRMSEKE 354 Query: 454 DFHALFMR 477 + +R Sbjct: 355 AYQYFVLR 362 >At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger) family protein contains similarity to polycomb-M33 interacting protein Ring1B [Mus musculus] GI:2239142; contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger) Length = 486 Score = 29.1 bits (62), Expect = 3.4 Identities = 21/64 (32%), Positives = 31/64 (48%) Frame = -2 Query: 427 VCISGILDSTPSNLDRLHHLRCECIYPETRPGPHETVPAGRCMCCP*LGSSDRDPRSHVL 248 +C+ GI+ T + ++ LH ECI R G +E PA R C D DP+ L Sbjct: 138 ICL-GIIKKTRTVMECLHRFCRECIDKSMRLGNNE-CPACRKHCASRRSLRD-DPKFDAL 194 Query: 247 VVAM 236 + A+ Sbjct: 195 IAAL 198 >At5g18490.1 68418.m02181 expressed protein Length = 553 Score = 29.1 bits (62), Expect = 3.4 Identities = 11/23 (47%), Positives = 13/23 (56%) Frame = -1 Query: 434 CSCMYLWNSGFDSQQSRSTSSPP 366 C C Y WN GF +S S+ S P Sbjct: 3 CDCFY-WNKGFSELESESSESKP 24 >At3g54980.1 68416.m06100 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 851 Score = 27.9 bits (59), Expect = 7.8 Identities = 13/35 (37%), Positives = 19/35 (54%) Frame = +1 Query: 37 PELPISERKVSGFKPSGSLSRDLDLHQGKHSDGYR 141 P PI +SGF+ G++ LDL++ DG R Sbjct: 687 PSQPIYNSLISGFRNLGNMVAALDLYKKMLKDGLR 721 >At1g34060.1 68414.m04222 alliinase family protein contains Pfam profiles: PF04864 allinase C-terminal domain, PF04863 alliinase EGF-like domain Length = 463 Score = 27.9 bits (59), Expect = 7.8 Identities = 12/47 (25%), Positives = 25/47 (53%) Frame = +2 Query: 14 FHWHIVDDQSFPYQSERFPDLSRLGAYHETLIYTKENIQTVIDHARN 154 F W +V D++ + +RF L+ +G ET ++ + ++ V+ N Sbjct: 294 FGWGLVKDKAIYEKMDRFIRLTSMGVSKETQLHVLQLLKVVVGDGGN 340 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,952,879 Number of Sequences: 28952 Number of extensions: 422065 Number of successful extensions: 1105 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 1076 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1103 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1712086600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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