Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0215
(574 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 138 2e-33
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 132 2e-31
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 130 6e-31
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 126 1e-29
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 113 6e-26
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 108 3e-24
At1g80600.1 68414.m09457 acetylornithine aminotransferase, mitoc... 29 2.2
At3g45670.1 68416.m04935 protein kinase-related contains eukaryo... 29 2.9
At2g45830.1 68415.m05699 expressed protein 29 2.9
At4g09670.1 68417.m01588 oxidoreductase family protein similar t... 27 8.9
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 138 bits (334), Expect = 2e-33
Identities = 59/87 (67%), Positives = 74/87 (85%)
Frame = +1
Query: 4 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 183
+RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP+GLFI++ DKG + DVLKN
Sbjct: 107 ERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISLKDKGKILDVLKN 166
Query: 184 WPDTDVRAIVVTDGERILGLGDLGHAG 264
WP+ +++ IVVTDGERILGLGDLG G
Sbjct: 167 WPERNIQVIVVTDGERILGLGDLGCQG 193
Score = 124 bits (300), Expect = 3e-29
Identities = 55/86 (63%), Positives = 68/86 (79%)
Frame = +3
Query: 249 LGACWMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSILDDPLYIGLRQRRVRGPDY 428
LG MGIPVGKLALY+ALGG++P CLP+TIDVGTN + +L+D YIGLRQ+R G +Y
Sbjct: 189 LGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEY 248
Query: 429 DELLDEFMLAVVRRYGQNCLVQFEDF 506
ELL+EFM AV + YG+ L+QFEDF
Sbjct: 249 SELLNEFMSAVKQNYGEKVLIQFEDF 274
Score = 31.1 bits (67), Expect = 0.55
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +2
Query: 509 NANAFRLLQKYRNKYCTFNDDI 574
N NAF LL KY + + FNDDI
Sbjct: 276 NHNAFELLAKYSDTHLVFNDDI 297
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 132 bits (319), Expect = 2e-31
Identities = 55/87 (63%), Positives = 72/87 (82%)
Frame = +1
Query: 4 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 183
+RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+GL+I++ +KG + +VLKN
Sbjct: 114 ERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKN 173
Query: 184 WPDTDVRAIVVTDGERILGLGDLGHAG 264
WP ++ IVVTDGERILGLGDLG G
Sbjct: 174 WPQRGIQVIVVTDGERILGLGDLGCQG 200
Score = 121 bits (291), Expect = 4e-28
Identities = 55/86 (63%), Positives = 66/86 (76%)
Frame = +3
Query: 249 LGACWMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSILDDPLYIGLRQRRVRGPDY 428
LG MGIPVGKL+LYTALGGI+P CLPITIDVGTN + +L++ YIGL+Q+R G +Y
Sbjct: 196 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANGEEY 255
Query: 429 DELLDEFMLAVVRRYGQNCLVQFEDF 506
E L EFM AV + YG+ LVQFEDF
Sbjct: 256 AEFLQEFMCAVKQNYGEKVLVQFEDF 281
Score = 29.1 bits (62), Expect = 2.2
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2
Query: 509 NANAFRLLQKYRNKYCTFNDDI 574
N +AF LL KY + + FNDDI
Sbjct: 283 NHHAFELLSKYCSSHLVFNDDI 304
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 130 bits (314), Expect = 6e-31
Identities = 53/87 (60%), Positives = 73/87 (83%)
Frame = +1
Query: 4 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 183
+RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL+I++++KG + +VLKN
Sbjct: 114 ERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISLNEKGKILEVLKN 173
Query: 184 WPDTDVRAIVVTDGERILGLGDLGHAG 264
WP ++ IVVTDGERILGLGDLG G
Sbjct: 174 WPQRGIQVIVVTDGERILGLGDLGCQG 200
Score = 123 bits (297), Expect = 7e-29
Identities = 57/86 (66%), Positives = 66/86 (76%)
Frame = +3
Query: 249 LGACWMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSILDDPLYIGLRQRRVRGPDY 428
LG MGIPVGKL+LYTALGGI+P CLPITIDVGTN + +L+D YIGL+QRR G +Y
Sbjct: 196 LGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEY 255
Query: 429 DELLDEFMLAVVRRYGQNCLVQFEDF 506
E L EFM AV + YG+ LVQFEDF
Sbjct: 256 AEFLHEFMCAVKQNYGEKVLVQFEDF 281
Score = 31.1 bits (67), Expect = 0.55
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +2
Query: 509 NANAFRLLQKYRNKYCTFNDDI 574
N NAF LL KY + + FNDDI
Sbjct: 283 NHNAFDLLSKYSDSHLVFNDDI 304
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 126 bits (303), Expect = 1e-29
Identities = 50/87 (57%), Positives = 73/87 (83%)
Frame = +1
Query: 4 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 183
+ NE LFY+ + D+V E++P++YTPTVG ACQK+G ++ RP+GLFI++ +KG +++VL+N
Sbjct: 172 ETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRN 231
Query: 184 WPDTDVRAIVVTDGERILGLGDLGHAG 264
WP+ +++ IVVTDGERILGLGDLG G
Sbjct: 232 WPEKNIQVIVVTDGERILGLGDLGCQG 258
Score = 124 bits (298), Expect = 6e-29
Identities = 54/86 (62%), Positives = 67/86 (77%)
Frame = +3
Query: 249 LGACWMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSILDDPLYIGLRQRRVRGPDY 428
LG MGIPVGKL+LYTALGG++P CLP+TIDVGTN + +L+D YIGLRQRR G +Y
Sbjct: 254 LGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEY 313
Query: 429 DELLDEFMLAVVRRYGQNCLVQFEDF 506
EL+ EFM AV + YG+ ++QFEDF
Sbjct: 314 SELMHEFMTAVKQNYGEKVVIQFEDF 339
Score = 29.5 bits (63), Expect = 1.7
Identities = 13/22 (59%), Positives = 14/22 (63%)
Frame = +2
Query: 509 NANAFRLLQKYRNKYCTFNDDI 574
N NAF LL KY + FNDDI
Sbjct: 341 NHNAFDLLAKYGTTHLVFNDDI 362
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 113 bits (273), Expect = 6e-26
Identities = 48/87 (55%), Positives = 62/87 (71%)
Frame = +1
Query: 4 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 183
DRNE L+YR + DN+ + PI+YTPTVGL CQ + +YRRPRG++ + DKG + ++ N
Sbjct: 114 DRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYN 173
Query: 184 WPDTDVRAIVVTDGERILGLGDLGHAG 264
WP V IV+TDG RILGLGDLG G
Sbjct: 174 WPAPQVDMIVITDGSRILGLGDLGVQG 200
Score = 93.1 bits (221), Expect = 1e-19
Identities = 43/86 (50%), Positives = 59/86 (68%)
Frame = +3
Query: 249 LGACWMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSILDDPLYIGLRQRRVRGPDY 428
LG +GIP+GKL +Y A GI P + LPI +DVGTN + +L + LY+G+RQ R+ G +Y
Sbjct: 196 LGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDLYLGVRQPRLEGEEY 255
Query: 429 DELLDEFMLAVVRRYGQNCLVQFEDF 506
E++DEFM A R+ +VQFEDF
Sbjct: 256 LEIIDEFMEAAFTRW-PKAVVQFEDF 280
Score = 32.3 bits (70), Expect = 0.24
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 518 AFRLLQKYRNKYCTFNDDI 574
AF L++YR K+C FNDD+
Sbjct: 285 AFGTLERYRKKFCMFNDDV 303
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 108 bits (259), Expect = 3e-24
Identities = 46/87 (52%), Positives = 62/87 (71%)
Frame = +1
Query: 4 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 183
DRNE ++Y+ + +N+ E PIVYTPTVGL CQ + ++RRPRG++ + D+G + ++ N
Sbjct: 121 DRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYN 180
Query: 184 WPDTDVRAIVVTDGERILGLGDLGHAG 264
WP V IVVTDG RILGLGDLG G
Sbjct: 181 WPAEQVDMIVVTDGSRILGLGDLGVHG 207
Score = 95.9 bits (228), Expect = 2e-20
Identities = 45/86 (52%), Positives = 61/86 (70%)
Frame = +3
Query: 249 LGACWMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSILDDPLYIGLRQRRVRGPDY 428
LG +GI VGKL LY A GI P + LP+ IDVGTN + + +DP+Y+GL+QRR+ DY
Sbjct: 203 LGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLEDDDY 262
Query: 429 DELLDEFMLAVVRRYGQNCLVQFEDF 506
+++DEFM AV R+ + +VQFEDF
Sbjct: 263 IDVIDEFMEAVYTRW-PHVIVQFEDF 287
Score = 30.3 bits (65), Expect = 0.95
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = +2
Query: 518 AFRLLQKYRNKYCTFNDDI 574
AF+LLQ+YR Y FNDD+
Sbjct: 292 AFKLLQRYRCTYRMFNDDV 310
>At1g80600.1 68414.m09457 acetylornithine aminotransferase,
mitochondrial, putative / acetylornithine transaminase,
putative / AOTA, putative / ACOAT, putative similar to
SP|O04866 Acetylornithine aminotransferase,
mitochondrial precursor (EC 2.6.1.11) (ACOAT)
(Acetylornithine transaminase) (AOTA) {Alnus glutinosa};
contains Pfam profile PF00202: aminotransferase, class
III
Length = 457
Score = 29.1 bits (62), Expect = 2.2
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = -3
Query: 398 EPDVQRVVQNRLGVRTHVDSDGQTLMRLDASERGVEREFANR 273
+PD R V + GV HV + T+ +++ ++R V FA+R
Sbjct: 112 DPDWLRAVTEQAGVLAHVSNVYYTIPQIELAKRLVASSFADR 153
>At3g45670.1 68416.m04935 protein kinase-related contains eukaryotic
protein kinase domain, INTERPRO:IPR000719
Length = 379
Score = 28.7 bits (61), Expect = 2.9
Identities = 19/56 (33%), Positives = 27/56 (48%), Gaps = 4/56 (7%)
Frame = +1
Query: 223 GERILG-LGDLGHAGWASLLANSRSTPRSEASSRISVCPS---LSTWVRTPSRFWT 378
G+R+ G GDL H W L+ +S TP + S R+S + V+ P WT
Sbjct: 302 GKRVWGEYGDLAHDDWVDLIGHSDLTP--QISIRLSAEAQDFLMRCLVKEPGSRWT 355
>At2g45830.1 68415.m05699 expressed protein
Length = 523
Score = 28.7 bits (61), Expect = 2.9
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +1
Query: 280 ANSRSTPRSEASSRISVCPSLSTWVRTPSRFW 375
+ + PRS + SRIS CPS W+ R W
Sbjct: 103 SRNNDKPRS-SHSRISTCPSYFRWIHEDLRPW 133
>At4g09670.1 68417.m01588 oxidoreductase family protein similar to
AX110P [Daucus carota] GI:285739; contains Pfam profiles
PF01408: Oxidoreductase family NAD-binding Rossmann
fold, PF02894: Oxidoreductase family C-terminal
alpha/beta domain
Length = 362
Score = 27.1 bits (57), Expect = 8.9
Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 4/27 (14%)
Frame = +1
Query: 238 GLGDLGHAGW----ASLLANSRSTPRS 306
GLG LG AGW A+LLAN+ P++
Sbjct: 181 GLGALGDAGWYAIRATLLANNFELPKT 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,890,432
Number of Sequences: 28952
Number of extensions: 251052
Number of successful extensions: 792
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -