Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0202
(629 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast... 123 1e-28
At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondri... 119 2e-27
At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal, puta... 113 9e-26
At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondri... 113 9e-26
At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal ident... 105 3e-23
At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative stron... 41 6e-04
At2g36700.1 68415.m04503 pectinesterase family protein contains ... 30 1.5
At4g38670.1 68417.m05475 pathogenesis-related thaumatin family p... 29 1.9
At1g55540.1 68414.m06356 proline-rich family protein contains pr... 29 1.9
At2g18380.1 68415.m02141 zinc finger (GATA type) family protein 29 2.5
At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing ... 29 3.4
At1g51540.1 68414.m05801 kelch repeat-containing protein contain... 28 5.9
At1g23180.1 68414.m02896 armadillo/beta-catenin repeat family pr... 28 5.9
At5g28823.1 68418.m03544 hypothetical protein 27 7.8
At5g04320.1 68418.m00424 expressed protein 27 7.8
>At3g47520.1 68416.m05168 malate dehydrogenase [NAD], chloroplast
(MDH) identical to chloroplast NAD-malate dehydrogenase
[Arabidopsis thaliana] GI:3256066; contains InterPro
entry IPR001236: Lactate/malate dehydrogenase; contains
Pfam profiles PF00056: lactate/malate dehydrogenase, NAD
binding domain and PF02866: lactate/malate
dehydrogenase, alpha/beta C-terminal domain
Length = 403
Score = 123 bits (296), Expect = 1e-28
Identities = 55/94 (58%), Positives = 73/94 (77%)
Frame = +3
Query: 255 AADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIVRDI 434
AADLSH NTP++V GP EL+ +KD +VVVIPAGVPRKPGMTRDDLFN NA+IV+ +
Sbjct: 123 AADLSHCNTPSQVRDFTGPSELADCLKDVNVVVIPAGVPRKPGMTRDDLFNINANIVKTL 182
Query: 435 ALSIAQNAPKAIVAIITNPVNSTVPLPLKCSRRR 536
++A+N P A + II+NPVNSTVP+ + +++
Sbjct: 183 VEAVAENCPNAFIHIISNPVNSTVPIAAEVLKKK 216
Score = 45.2 bits (102), Expect = 4e-05
Identities = 20/26 (76%), Positives = 23/26 (88%)
Frame = +2
Query: 509 IASEVLKKAGVYDPNRVLGVTTLDVV 586
IA+EVLKK GVYDP ++ GVTTLDVV
Sbjct: 208 IAAEVLKKKGVYDPKKLFGVTTLDVV 233
Score = 38.3 bits (85), Expect = 0.004
Identities = 17/24 (70%), Positives = 21/24 (87%)
Frame = +1
Query: 166 IGQPLALLLKQNPLVTRLALYDIA 237
IGQPL+LL+K +PLV+ L LYDIA
Sbjct: 94 IGQPLSLLIKMSPLVSTLHLYDIA 117
>At3g15020.1 68416.m01900 malate dehydrogenase [NAD], mitochondrial,
putative similar to mitochondrial NAD-dependent malate
dehydrogenase GB:CAA10320 SP|Q9ZP06 [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 119 bits (286), Expect = 2e-27
Identities = 52/89 (58%), Positives = 75/89 (84%)
Frame = +3
Query: 246 PRRAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 425
P AAD+ H+NT ++VSG+ G ++L A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV
Sbjct: 67 PGVAADVGHINTRSQVSGYMGDDDLGKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIV 126
Query: 426 RDIALSIAQNAPKAIVAIITNPVNSTVPL 512
++++++IA+ P+A+V +I+NPVNSTVP+
Sbjct: 127 KNLSIAIAKYCPQALVNMISNPVNSTVPI 155
Score = 44.0 bits (99), Expect = 8e-05
Identities = 26/64 (40%), Positives = 37/64 (57%), Gaps = 2/64 (3%)
Frame = +1
Query: 52 MFSRALKPAALAVQNGA--KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLAL 225
MF + +A V+ G + F++ S + K IGQPL+LL+K NPLV+ L+L
Sbjct: 1 MFRSMIVRSASPVKQGLLRRGFASESVPDRKVVILGAAGGIGQPLSLLMKLNPLVSSLSL 60
Query: 226 YDIA 237
YDIA
Sbjct: 61 YDIA 64
Score = 40.7 bits (91), Expect = 8e-04
Identities = 20/44 (45%), Positives = 25/44 (56%)
Frame = +2
Query: 455 CTQSYCGHHHKPC*LYSAIASEVLKKAGVYDPNRVLGVTTLDVV 586
C Q+ P IA+E+ KKAG YD ++ GVTTLDVV
Sbjct: 137 CPQALVNMISNPVNSTVPIAAEIFKKAGTYDEKKLFGVTTLDVV 180
>At2g22780.1 68415.m02702 malate dehydrogenase, glyoxysomal,
putative strong similarity to glyoxysomal malate
dehydrogenase (EC 1.1.1.37) SP|P19446 {Citrullus
lanatus}, SP|P46488 {Cucumis sativus}, [Medicago sativa]
GI:2827078, SP|Q42972 {Oryza sativa}, SP|Q9ZP05
{Arabidopsis thaliana}, SP|P37228 {Glycine max};
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 354
Score = 113 bits (272), Expect = 9e-26
Identities = 55/89 (61%), Positives = 67/89 (75%)
Frame = +3
Query: 246 PRRAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 425
P AD+SHM+T A V G G +L A+ D+V+IPAGVPRKPGMTRDDLFN NA IV
Sbjct: 80 PGVTADISHMDTSAVVRGFLGQPQLEEALTGMDLVIIPAGVPRKPGMTRDDLFNINAGIV 139
Query: 426 RDIALSIAQNAPKAIVAIITNPVNSTVPL 512
R ++ +IA+ PKAIV II+NPVNSTVP+
Sbjct: 140 RTLSEAIAKCCPKAIVNIISNPVNSTVPI 168
Score = 44.8 bits (101), Expect = 5e-05
Identities = 20/40 (50%), Positives = 27/40 (67%)
Frame = +2
Query: 509 IASEVLKKAGVYDPNRVLGVTTLDVVALPPSSARINGVDP 628
IA+EV KKAG +DP +++GVT LDVV A + +DP
Sbjct: 168 IAAEVFKKAGTFDPKKLMGVTMLDVVRANTFVAEVMSLDP 207
Score = 40.3 bits (90), Expect = 0.001
Identities = 19/35 (54%), Positives = 23/35 (65%)
Frame = +1
Query: 133 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIA 237
FK IGQPLA+L+K NPLV+ L LYD+A
Sbjct: 43 FKVAILGAAGGIGQPLAMLMKMNPLVSVLHLYDVA 77
>At1g53240.1 68414.m06033 malate dehydrogenase [NAD], mitochondrial
identical to mitochondrial NAD-dependent malate
dehydrogenase GI:3929649 SP|Q9ZP06 from [Arabidopsis
thaliana]; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 341
Score = 113 bits (272), Expect = 9e-26
Identities = 50/89 (56%), Positives = 71/89 (79%)
Frame = +3
Query: 246 PRRAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 425
P AAD+ H+NT ++V G+ G + L+ A++ AD+V+IPAGVPRKPGMTRDDLFN NA IV
Sbjct: 67 PGVAADVGHINTRSEVVGYMGDDNLAKALEGADLVIIPAGVPRKPGMTRDDLFNINAGIV 126
Query: 426 RDIALSIAQNAPKAIVAIITNPVNSTVPL 512
+++ +IA+ P A++ +I+NPVNSTVP+
Sbjct: 127 KNLCTAIAKYCPHALINMISNPVNSTVPI 155
Score = 44.0 bits (99), Expect = 8e-05
Identities = 23/45 (51%), Positives = 29/45 (64%)
Frame = +1
Query: 103 KNFSTTSQRNFKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDIA 237
++FS+ S K IGQPLALL+K NPLV+ L+LYDIA
Sbjct: 20 RSFSSGSVPERKVAILGAAGGIGQPLALLMKLNPLVSSLSLYDIA 64
Score = 40.7 bits (91), Expect = 8e-04
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = +2
Query: 509 IASEVLKKAGVYDPNRVLGVTTLDVV 586
IA+E+ KKAG+YD ++ GVTTLDVV
Sbjct: 155 IAAEIFKKAGMYDEKKLFGVTTLDVV 180
>At5g09660.1 68418.m01117 malate dehydrogenase, glyoxysomal
identical to SP|Q9ZP05; identical to cDNA microbody
NAD-dependent malate dehydrogenase GI:3929650
Length = 354
Score = 105 bits (251), Expect = 3e-23
Identities = 49/89 (55%), Positives = 64/89 (71%)
Frame = +3
Query: 246 PRRAADLSHMNTPAKVSGHKGPEELSAAIKDADVVVIPAGVPRKPGMTRDDLFNTNASIV 425
P AD+SHM+T A V G G ++L A+ D+V+IPAG+PRKPGMTRDDLF NA IV
Sbjct: 80 PGVTADVSHMDTGAVVRGFLGAKQLEDALTGMDLVIIPAGIPRKPGMTRDDLFKINAGIV 139
Query: 426 RDIALSIAQNAPKAIVAIITNPVNSTVPL 512
+ + +A+ P AIV +I+NPVNSTVP+
Sbjct: 140 KTLCEGVAKCCPNAIVNLISNPVNSTVPI 168
Score = 50.0 bits (114), Expect = 1e-06
Identities = 23/40 (57%), Positives = 28/40 (70%)
Frame = +2
Query: 509 IASEVLKKAGVYDPNRVLGVTTLDVVALPPSSARINGVDP 628
IA+EV KKAG YDP ++LGVTTLDV A + G+DP
Sbjct: 168 IAAEVFKKAGTYDPKKLLGVTTLDVARANTFVAEVLGLDP 207
Score = 35.5 bits (78), Expect = 0.029
Identities = 17/34 (50%), Positives = 21/34 (61%)
Frame = +1
Query: 133 FKXXXXXXXXXIGQPLALLLKQNPLVTRLALYDI 234
FK IGQ L+LL+K NPLV+ L LYD+
Sbjct: 43 FKVAILGAAGGIGQSLSLLMKMNPLVSLLHLYDV 76
>At4g17260.1 68417.m02595 L-lactate dehydrogenase, putative strong
similarity to L-lactate dehydrogenase from Lycopersicon
esculentum (GI:1620970, GI:1620972), Hordeum vulgare
(SP|P22988, SP|P22989); contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 353
Score = 41.1 bits (92), Expect = 6e-04
Identities = 17/53 (32%), Positives = 34/53 (64%)
Frame = +3
Query: 339 ADVVVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVN 497
+D+ ++ AG + PG +R +L N ++ R I +A+ +P +I+ I++NPV+
Sbjct: 109 SDLCIVTAGARQNPGESRLNLLQRNVALFRHIIPPLAKASPDSILIIVSNPVD 161
>At2g36700.1 68415.m04503 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 333
Score = 29.9 bits (64), Expect = 1.5
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -1
Query: 470 NSFGCILSNGQSNVTD-NGSISVE*VITGHSRFTGYSCWDDYNISIF 333
N+ GCI +NG+ + D G + V ITG +R W Y IF
Sbjct: 217 NTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIF 263
>At4g38670.1 68417.m05475 pathogenesis-related thaumatin family
protein similar to receptor serine/threonine kinase PR5K
[Arabidopsis thaliana] GI:1235680; contains Pfam profile
PF00314: Thaumatin family
Length = 281
Score = 29.5 bits (63), Expect = 1.9
Identities = 34/127 (26%), Positives = 50/127 (39%), Gaps = 5/127 (3%)
Frame = -1
Query: 566 RRARGSGRTLPPS*ALQRQWHCRV--NRVC--DDGHNSFGCILSN-GQSNVTDNGSISVE 402
R +RG +T+ A+ W R+ +C D SF C+ + G V +GS +
Sbjct: 54 RLSRGKSKTV----AIPESWSGRLWARTLCSQDRSSGSFVCLTGDCGSGKVECSGSGAKP 109
Query: 401 *VITGHSRFTGYSCWDDYNISIFDGC**LLRSLVAADLGWGVHVGKVGCTPGVTGAMSYK 222
G D Y++S+ DG + L + G G GC + GA
Sbjct: 110 PATLAEFTLNGTGGLDFYDVSLVDGYNLPMLILPKKIVIGGC--GATGCLVDLNGACPRD 167
Query: 221 ASLVTRG 201
LVTRG
Sbjct: 168 LKLVTRG 174
>At1g55540.1 68414.m06356 proline-rich family protein contains
proline rich extensin domain, INTERPRO:IPR002965
Length = 915
Score = 29.5 bits (63), Expect = 1.9
Identities = 24/113 (21%), Positives = 42/113 (37%), Gaps = 1/113 (0%)
Frame = +3
Query: 177 FGPSTE-AESSGDQAGFIRHSACDPRRAADLSHMNTPAKVSGHKGPEELSAAIKDADVVV 353
F PS ++ S DQA R + S +TP + A +
Sbjct: 457 FPPSAPTSQVSSDQASATSSLTDSSRLFSSTSLSSTPPITPPDAFQSPQVSTPSSAVPIT 516
Query: 354 IPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPL 512
P P+KP + +T +++ + QN P + + I+NP + P+
Sbjct: 517 EPVSEPKKPEAQSSSILSTQSTVDSVANATKTQNEPLPVKSEISNPGTTVTPV 569
>At2g18380.1 68415.m02141 zinc finger (GATA type) family protein
Length = 207
Score = 29.1 bits (62), Expect = 2.5
Identities = 17/45 (37%), Positives = 24/45 (53%)
Frame = +3
Query: 195 AESSGDQAGFIRHSACDPRRAADLSHMNTPAKVSGHKGPEELSAA 329
A++S + G + HS PRR A +TP +G KGP+ L A
Sbjct: 75 AKTSSYKKGGVAHSL--PRRCASCDTTSTPLWRNGPKGPKSLCNA 117
>At4g21030.1 68417.m03042 Dof-type zinc finger domain-containing
protein prolamin box binding factor, Zea mays,
PID:g2393775
Length = 194
Score = 28.7 bits (61), Expect = 3.4
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +3
Query: 12 SNIHVYTR*TNNENVLPRPKTCRPC 86
+N++V+T N NV+P P+ C C
Sbjct: 2 NNLNVFTNEDNEMNVMPPPRVCPRC 26
>At1g51540.1 68414.m05801 kelch repeat-containing protein contains
Pfam profile PF01344: Kelch motif
Length = 1036
Score = 27.9 bits (59), Expect = 5.9
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -1
Query: 398 VITGHSRFTGYSCWDDYNISIFDG 327
++T HS FT WD YN S+ DG
Sbjct: 314 LVTDHSSFTEKEAWDGYNKSL-DG 336
>At1g23180.1 68414.m02896 armadillo/beta-catenin repeat family
protein contains Pfam profile: PF00514
armadillo/beta-catenin-like repeat
Length = 834
Score = 27.9 bits (59), Expect = 5.9
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
Frame = +3
Query: 348 VVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQ-NAPKAIVAIITNPVNSTVPLPLKC 524
+V+ G+ + TR +ASI D+ +S + A K +V ++ N TV LP+
Sbjct: 430 LVLILGLADELAATRAAESIADASINEDMRVSFMEAGAVKPLVQLLANNNKETVKLPVIR 489
Query: 525 SRRRECTTRTAC 560
+ + +RT C
Sbjct: 490 ALKNLSLSRTVC 501
>At5g28823.1 68418.m03544 hypothetical protein
Length = 568
Score = 27.5 bits (58), Expect = 7.8
Identities = 20/54 (37%), Positives = 27/54 (50%), Gaps = 5/54 (9%)
Frame = +3
Query: 429 DIALSIAQNAP--KAIVAIIT---NPVNSTVPLPLKCSRRRECTTRTACSASPL 575
D A + +Q AP K+++ I T PV PLPL+C +E A S PL
Sbjct: 80 DSATTKSQLAPLVKSLIEIATPTIEPVVQRSPLPLRCRLSKELGRFLATSVLPL 133
>At5g04320.1 68418.m00424 expressed protein
Length = 419
Score = 27.5 bits (58), Expect = 7.8
Identities = 17/63 (26%), Positives = 27/63 (42%)
Frame = +3
Query: 348 VVIPAGVPRKPGMTRDDLFNTNASIVRDIALSIAQNAPKAIVAIITNPVNSTVPLPLKCS 527
V+ G+P + T DD+ + R + I K V NPV +V +C+
Sbjct: 151 VIDTTGIPEEICQTEDDI--DKGVVSRGVNQDIDNVVNKKFVPDAANPVKESVHRKRQCT 208
Query: 528 RRR 536
RR+
Sbjct: 209 RRQ 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,009,538
Number of Sequences: 28952
Number of extensions: 343489
Number of successful extensions: 936
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 898
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 936
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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