BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0195 (582 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 155 6e-39 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 124 7e-30 SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 120 2e-28 SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|... 109 2e-25 SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccha... 100 2e-22 SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizos... 81 2e-16 SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 60 2e-10 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 58 9e-10 SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 57 2e-09 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 2.0 SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 27 2.6 SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyce... 27 2.6 SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 26 4.6 SPBC215.03c |csn1||COP9/signalosome complex subunit Csn1|Schizos... 25 8.1 >SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosaccharomyces pombe|chr 1|||Manual Length = 1168 Score = 155 bits (375), Expect = 6e-39 Identities = 72/83 (86%), Positives = 78/83 (93%) Frame = +1 Query: 259 PGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFEPAPPGS 438 PGDIL+FLTGQEEIDT+CEILYER K LG +PEL+ILPVYSALPSE+Q+RIFEPAPPG Sbjct: 719 PGDILVFLTGQEEIDTSCEILYERSKMLGDSIPELVILPVYSALPSEIQSRIFEPAPPGG 778 Query: 439 RKVVIATNIAETSLTIDGIYYVV 507 RKVVIATNIAETSLTIDGIYYVV Sbjct: 779 RKVVIATNIAETSLTIDGIYYVV 801 Score = 142 bits (343), Expect = 4e-35 Identities = 66/90 (73%), Positives = 79/90 (87%), Gaps = 1/90 (1%) Frame = +2 Query: 2 AHERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEV 181 AHERT+ TDVLFGLLK V KRP+LKLIVTSATLDA +FS YF++ PIFTIPGR++PVE+ Sbjct: 633 AHERTVATDVLFGLLKGTVLKRPDLKLIVTSATLDAERFSSYFYKCPIFTIPGRSYPVEI 692 Query: 182 LYTKEPETDYLDASLITVMQIHL-KGPRGI 268 +YTK+PE DYLDA+L+TVMQIHL +GP I Sbjct: 693 MYTKQPEADYLDAALMTVMQIHLSEGPGDI 722 Score = 40.7 bits (91), Expect = 2e-04 Identities = 17/24 (70%), Positives = 20/24 (83%) Frame = +3 Query: 510 PGFVKQKVYNSKTGMDSLVVTPIS 581 PGFVKQ ++ K GMDSL+VTPIS Sbjct: 803 PGFVKQSCFDPKLGMDSLIVTPIS 826 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 124 bits (300), Expect = 7e-30 Identities = 55/90 (61%), Positives = 74/90 (82%), Gaps = 1/90 (1%) Frame = +2 Query: 2 AHERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEV 181 AHERT+ TD+L GL+K+ +RP+LK+IV SATLDA KF +YFF+AP+ +PGRT+PVE+ Sbjct: 199 AHERTLATDILMGLMKRLATRRPDLKIIVMSATLDAKKFQKYFFDAPLLAVPGRTYPVEI 258 Query: 182 LYTKEPETDYLDASLITVMQIHL-KGPRGI 268 YT+EPE DYL+A+L TV+QIH+ +GP I Sbjct: 259 YYTQEPERDYLEAALRTVLQIHVEEGPGDI 288 Score = 85.8 bits (203), Expect = 4e-18 Identities = 49/93 (52%), Positives = 59/93 (63%), Gaps = 7/93 (7%) Frame = +1 Query: 250 EGPPGDILLFLTGQEEIDTACEILYERMKSLGPD--VPELIILPVYSALPSEMQTRIFEP 423 E PGDIL+FLTG+EEI+ AC + L + L + P+Y +LP Q RIFEP Sbjct: 282 EEGPGDILVFLTGEEEIEDACRKITLEADDLVREGAAGPLKVYPLYGSLPPNQQQRIFEP 341 Query: 424 APPGS-----RKVVIATNIAETSLTIDGIYYVV 507 P + RKVVI+TNIAETSLTIDGI YVV Sbjct: 342 TPEDTKSGYGRKVVISTNIAETSLTIDGIVYVV 374 Score = 35.5 bits (78), Expect = 0.006 Identities = 14/24 (58%), Positives = 20/24 (83%) Frame = +3 Query: 510 PGFVKQKVYNSKTGMDSLVVTPIS 581 PGF KQK+YN + ++SL+V+PIS Sbjct: 376 PGFSKQKIYNPRIRVESLLVSPIS 399 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 120 bits (288), Expect = 2e-28 Identities = 48/88 (54%), Positives = 70/88 (79%) Frame = +2 Query: 2 AHERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEV 181 AHERT+HTD+LFGL+K + RP+LK++++SAT+DA KFS YF EAP+F +PGR +PV++ Sbjct: 541 AHERTLHTDILFGLVKDIARFRPDLKVLISSATIDAEKFSAYFDEAPVFYVPGRRYPVDI 600 Query: 182 LYTKEPETDYLDASLITVMQIHLKGPRG 265 YT +PE +Y+ A++ T++QIH P G Sbjct: 601 YYTPQPEANYIQAAITTILQIHTTQPAG 628 Score = 113 bits (273), Expect = 1e-26 Identities = 49/84 (58%), Positives = 68/84 (80%) Frame = +1 Query: 256 PPGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFEPAPPG 435 P GDIL+FLTGQ+EI+ E + E + LG +PE+I+ P+Y+ LPSE+Q +IF+P PPG Sbjct: 626 PAGDILVFLTGQDEIELMSENMQELCRILGKRIPEIILCPIYANLPSELQAKIFDPTPPG 685 Query: 436 SRKVVIATNIAETSLTIDGIYYVV 507 +RKVV+ATNIAETS+TIDG+ +V+ Sbjct: 686 ARKVVLATNIAETSITIDGVNFVI 709 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/23 (65%), Positives = 18/23 (78%) Frame = +3 Query: 513 GFVKQKVYNSKTGMDSLVVTPIS 581 GFVKQ +YN +TGM+SLV P S Sbjct: 712 GFVKQNMYNPRTGMESLVSVPCS 734 >SPBC1711.17 |prp16|SPBC17G9.01|ATP-dependent RNA helicase Prp16|Schizosaccharomyces pombe|chr 2|||Manual Length = 1173 Score = 109 bits (263), Expect = 2e-25 Identities = 50/84 (59%), Positives = 66/84 (78%) Frame = +1 Query: 256 PPGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFEPAPPG 435 P GDIL+F+TGQE+I+ CEI+ +R+ L D P L ILP+YS +P+++Q +IF+ A PG Sbjct: 699 PAGDILVFMTGQEDIEATCEIIADRLNQLH-DAPRLSILPIYSQMPADLQAKIFDSAEPG 757 Query: 436 SRKVVIATNIAETSLTIDGIYYVV 507 RKVV+ATNIAETSLT+ GI YVV Sbjct: 758 VRKVVVATNIAETSLTVHGISYVV 781 Score = 109 bits (261), Expect = 4e-25 Identities = 46/88 (52%), Positives = 71/88 (80%) Frame = +2 Query: 2 AHERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEV 181 AHER+++TD+L GLLK+ + +R ++KL+VTSAT+++ KFS +F AP FTIPGRT+PV++ Sbjct: 614 AHERSLNTDILMGLLKKVLSRRRDIKLLVTSATMNSQKFSDFFGGAPQFTIPGRTYPVDI 673 Query: 182 LYTKEPETDYLDASLITVMQIHLKGPRG 265 ++ K P +DY++A++ V+QIHL P G Sbjct: 674 MFAKAPCSDYVEAAVRQVLQIHLSQPAG 701 Score = 35.5 bits (78), Expect = 0.006 Identities = 15/23 (65%), Positives = 19/23 (82%) Frame = +3 Query: 513 GFVKQKVYNSKTGMDSLVVTPIS 581 G+ K K+YNSK G+D+L VTPIS Sbjct: 784 GYCKLKMYNSKLGIDTLQVTPIS 806 >SPAC2G11.11c |prh1||ATP-dependent RNA helicase Prh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 719 Score = 100 bits (239), Expect = 2e-22 Identities = 46/88 (52%), Positives = 64/88 (72%) Frame = +2 Query: 2 AHERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEV 181 AHERT+ TD+L G +K+ ++KRP L++I+ SATL+A +FS++F A I I GR +PV++ Sbjct: 218 AHERTLMTDMLLGFVKKIIKKRPALRVIIMSATLNAERFSEFFDGAEICYISGRQYPVQI 277 Query: 182 LYTKEPETDYLDASLITVMQIHLKGPRG 265 YT PE DYLDA L T+ Q+H K P G Sbjct: 278 HYTYTPEPDYLDACLRTIFQLHTKLPPG 305 Score = 89.8 bits (213), Expect = 3e-19 Identities = 41/84 (48%), Positives = 60/84 (71%) Frame = +1 Query: 256 PPGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFEPAPPG 435 PPGDIL+FLTGQ+EI+ ++ K L ++P++ P++++LP E Q ++F PA Sbjct: 303 PPGDILVFLTGQDEIEALEALIKSYSKQLPSNLPQIQACPLFASLPQEQQLQVFLPALAN 362 Query: 436 SRKVVIATNIAETSLTIDGIYYVV 507 RKVV++TNIAETS+TI GI YV+ Sbjct: 363 HRKVVLSTNIAETSVTISGIRYVI 386 Score = 30.7 bits (66), Expect = 0.16 Identities = 15/31 (48%), Positives = 21/31 (67%) Frame = +3 Query: 489 RYILRGGPGFVKQKVYNSKTGMDSLVVTPIS 581 RY++ G +KQ +NSK G++SL V PIS Sbjct: 383 RYVIDTGLAKIKQ--FNSKLGLESLTVQPIS 411 >SPAC20H4.09 |||ATP-dependent RNA helicase, spliceosomal |Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 80.6 bits (190), Expect = 2e-16 Identities = 37/89 (41%), Positives = 64/89 (71%), Gaps = 2/89 (2%) Frame = +2 Query: 5 HERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFT--IPGRTFPVE 178 HERT+ TD+L G+LK+ ++KR + +L+++SA++DA K SQ+F + + T I G+ FPVE Sbjct: 149 HERTLSTDLLLGVLKRILEKRNDFRLVLSSASVDANKLSQFFGQDKVCTMSIEGKLFPVE 208 Query: 179 VLYTKEPETDYLDASLITVMQIHLKGPRG 265 L+ ++P +Y+D+++ TV+ I+ P G Sbjct: 209 TLFLQKPTENYVDSAIETVININSTYPPG 237 Score = 76.2 bits (179), Expect = 3e-15 Identities = 37/84 (44%), Positives = 55/84 (65%) Frame = +1 Query: 256 PPGDILLFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFEPAPPG 435 PPGDIL+FL+G++EI+ + + + + D L+ LP+++ L + Q R+F Sbjct: 235 PPGDILVFLSGRKEIEYCIKKIEDSLIHASEDCQTLVPLPLHAGLTVDEQMRVFNIYDGD 294 Query: 436 SRKVVIATNIAETSLTIDGIYYVV 507 RKV+ +TNIAETS+TIDGI YVV Sbjct: 295 FRKVIFSTNIAETSITIDGIVYVV 318 Score = 26.2 bits (55), Expect = 3.5 Identities = 11/23 (47%), Positives = 14/23 (60%) Frame = +3 Query: 513 GFVKQKVYNSKTGMDSLVVTPIS 581 GF KQ++YN T L+ PIS Sbjct: 321 GFNKQRIYNPYTRTSKLINVPIS 343 >SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1428 Score = 60.1 bits (139), Expect = 2e-10 Identities = 27/64 (42%), Positives = 43/64 (67%) Frame = +2 Query: 5 HERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEVL 184 HER+I +D+L + +++ P LK+I+ SATL+A KF YF + + TIPG+T+PV Sbjct: 761 HERSIDSDLLLIHVLHLLKQHPHLKIIIMSATLNAEKFQLYFEGSNLITIPGKTYPVHRF 820 Query: 185 YTKE 196 Y ++ Sbjct: 821 YLED 824 Score = 46.0 bits (104), Expect = 4e-06 Identities = 32/86 (37%), Positives = 46/86 (53%), Gaps = 6/86 (6%) Frame = +1 Query: 268 ILLFLTGQEEIDTACEILYERMKSLGPDVP------ELIILPVYSALPSEMQTRIFEPAP 429 +L+FL G EI R+KSL D+P + I ++S L S Q +F P Sbjct: 906 VLVFLPGISEI--------LRVKSLIEDMPMFRNHRKFCIYMLHSTLSSAQQQSVFNIPP 957 Query: 430 PGSRKVVIATNIAETSLTIDGIYYVV 507 G RK+V++TNIAET +TI + V+ Sbjct: 958 KGCRKIVLSTNIAETGVTIPDVTCVI 983 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 58.0 bits (134), Expect = 9e-10 Identities = 37/102 (36%), Positives = 53/102 (51%), Gaps = 14/102 (13%) Frame = +2 Query: 2 AHERTIHTDVLFGLLKQAVQKRPE----------LKLIVTSATLDAVKFSQ----YFFEA 139 AHER+++TD+L GLL + V+ R E LKLI+ SATL FS+ + Sbjct: 524 AHERSVNTDILLGLLSRIVRLRREMSKSDQKVKPLKLIIMSATLRVTDFSENKLLFSVPP 583 Query: 140 PIFTIPGRTFPVEVLYTKEPETDYLDASLITVMQIHLKGPRG 265 PI I R +PV + + + + DYL + V IH + P G Sbjct: 584 PIIKIDARQYPVSIHFNRTTKPDYLQDAFDKVCLIHKRLPAG 625 Score = 57.2 bits (132), Expect = 2e-09 Identities = 24/49 (48%), Positives = 36/49 (73%) Frame = +1 Query: 361 LIILPVYSALPSEMQTRIFEPAPPGSRKVVIATNIAETSLTIDGIYYVV 507 + +LP+YS L +E Q ++F+ +P G R ++ATN+AETS+TI I YVV Sbjct: 702 MYVLPLYSLLTTEDQMKVFDSSPEGHRMCIVATNVAETSITIPNIRYVV 750 Score = 35.5 bits (78), Expect = 0.006 Identities = 15/32 (46%), Positives = 24/32 (75%), Gaps = 1/32 (3%) Frame = +1 Query: 256 PPGDILLFLTGQEEIDTACEILYER-MKSLGP 348 P G IL+FLTGQ+E++ C++L +R ++S P Sbjct: 623 PAGSILVFLTGQQEVEQLCQMLRKRFVRSFRP 654 >SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1327 Score = 57.2 bits (132), Expect = 2e-09 Identities = 27/64 (42%), Positives = 40/64 (62%) Frame = +2 Query: 5 HERTIHTDVLFGLLKQAVQKRPELKLIVTSATLDAVKFSQYFFEAPIFTIPGRTFPVEVL 184 HER+I D+L LLK + + P LK+I+ SAT+++ F YF A I GRTFP++ Sbjct: 706 HERSIENDILLTLLKLVISRIPNLKVILMSATVNSDTFKYYFGNAGHLHIHGRTFPIKDY 765 Query: 185 YTKE 196 Y ++ Sbjct: 766 YIED 769 Score = 45.6 bits (103), Expect = 5e-06 Identities = 38/115 (33%), Positives = 56/115 (48%) Frame = +1 Query: 163 NLPRRSLVHQGTGDGLFGRLTDHGHADPPEGPPGDILLFLTGQEEIDTACEILYERMKSL 342 ++PRR L RL A+ G IL+FL G I C +KS Sbjct: 782 DVPRRKKKEYEIDYHLISRLVSSIDAELGSSS-GSILVFLPGVSNI-ARC---IREIKS- 835 Query: 343 GPDVPELIILPVYSALPSEMQTRIFEPAPPGSRKVVIATNIAETSLTIDGIYYVV 507 D + +LP++++L + Q R F+ RK++ ATNIAETS+TID + V+ Sbjct: 836 -KDGSKFEVLPLHASLNTSEQRRCFKTYT--KRKIICATNIAETSITIDDVVAVI 887 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.1 bits (57), Expect = 2.0 Identities = 14/44 (31%), Positives = 22/44 (50%) Frame = -3 Query: 382 STPAELSAPARPAPRTSFSRTISHTPCQSLLGQ*GTAGYPPGAL 251 + PA SAPA PAP + + + P S++ A PP ++ Sbjct: 1468 NAPAPSSAPAPPAPVSQLPPAVPNVPVPSMIP--SVAQQPPSSV 1509 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 26.6 bits (56), Expect = 2.6 Identities = 13/50 (26%), Positives = 21/50 (42%) Frame = +3 Query: 396 RDADPDLRAGAPRLQEGGDRHQHRGDVADHRRYILRGGPGFVKQKVYNSK 545 R D R+ R +E D QH D +H R R +++ +N + Sbjct: 171 RHRDRHHRSNQDRSRERSDNEQHSSDKREHSRRSYRNDRNNWRERTHNDR 220 >SPCC1620.05 |||Rab geranylgeranyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 344 Score = 26.6 bits (56), Expect = 2.6 Identities = 12/44 (27%), Positives = 18/44 (40%) Frame = +2 Query: 155 PGRTFPVEVLYTKEPETDYLDASLITVMQIHLKGPRGISCCSSL 286 P P+ + T E YL + + ++H P CC SL Sbjct: 248 PNSMTPLISMITIEERIQYLQKEIELIQELHEMEPENRWCCESL 291 >SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.8 bits (54), Expect = 4.6 Identities = 16/49 (32%), Positives = 22/49 (44%) Frame = +1 Query: 340 LGPDVPELIILPVYSALPSEMQTRIFEPAPPGSRKVVIATNIAETSLTI 486 LGP P+L IL V P Q +F G +V+ T I L++ Sbjct: 48 LGPQPPQLEILEVGDLAPDRFQ-GLFNLHYSGDASLVLQTKIRANPLSV 95 >SPBC215.03c |csn1||COP9/signalosome complex subunit Csn1|Schizosaccharomyces pombe|chr 2|||Manual Length = 422 Score = 25.0 bits (52), Expect = 8.1 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +1 Query: 460 NIAETSLTIDGIYYVVALASLNRKST 537 NIA+TSL Y+ LA++N ++T Sbjct: 378 NIAQTSLLYSKALYLQTLAAMNTENT 403 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.137 0.404 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,202,061 Number of Sequences: 5004 Number of extensions: 45457 Number of successful extensions: 188 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 181 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 250133048 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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