BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0184 (607 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 140 1e-34 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 133 2e-32 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 117 1e-27 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 114 8e-27 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 86 3e-18 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 55 7e-09 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 46 6e-06 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 33 0.032 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 31 0.13 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.70 SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar... 25 6.5 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 25 6.5 SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|... 25 8.6 SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces pomb... 25 8.6 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 25 8.6 SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 25 8.6 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 25 8.6 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 140 bits (340), Expect = 1e-34 Identities = 65/97 (67%), Positives = 77/97 (79%) Frame = +3 Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416 P DAKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120 Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527 VL KM+ETAEAYLG V +AV+TVPAYFNDS+ + K Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATK 157 Score = 118 bits (285), Expect = 5e-28 Identities = 54/62 (87%), Positives = 59/62 (95%) Frame = +1 Query: 67 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 246 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 247 TI 252 TI Sbjct: 64 TI 65 Score = 65.3 bits (152), Expect = 7e-12 Identities = 31/33 (93%), Positives = 32/33 (96%) Frame = +2 Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607 QRQATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 152 QRQATKDAGLIAGLNVLRIINEPTAAAIAYGLD 184 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 133 bits (321), Expect = 2e-32 Identities = 61/97 (62%), Positives = 75/97 (77%) Frame = +3 Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416 P DAKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120 Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527 VL KM+E+AEA+LG V +AV+TVPAYFNDS+ + K Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATK 157 Score = 118 bits (285), Expect = 5e-28 Identities = 54/62 (87%), Positives = 59/62 (95%) Frame = +1 Query: 67 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 246 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP+N Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHN 63 Query: 247 TI 252 TI Sbjct: 64 TI 65 Score = 65.3 bits (152), Expect = 7e-12 Identities = 31/33 (93%), Positives = 32/33 (96%) Frame = +2 Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607 QRQATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 152 QRQATKDAGLIAGLNVLRIINEPTAAAIAYGLD 184 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 117 bits (282), Expect = 1e-27 Identities = 54/92 (58%), Positives = 69/92 (75%) Frame = +3 Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416 P+ DAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+M Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124 Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSK 512 VLTKMKE +EA L K V+ AVITVPAYF+DS+ Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPAYFSDSQ 156 Score = 105 bits (253), Expect = 4e-24 Identities = 47/62 (75%), Positives = 54/62 (87%) Frame = +1 Query: 67 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 246 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP N Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRN 67 Query: 247 TI 252 T+ Sbjct: 68 TV 69 Score = 63.3 bits (147), Expect = 3e-11 Identities = 30/33 (90%), Positives = 31/33 (93%) Frame = +2 Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607 QR ATKDAG I+GLNVLRIINEPTAAAIAYGLD Sbjct: 156 QRAATKDAGAIAGLNVLRIINEPTAAAIAYGLD 188 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 114 bits (275), Expect = 8e-27 Identities = 51/97 (52%), Positives = 73/97 (75%) Frame = +3 Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416 P+ D KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+M Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527 +L+KMK+TAEAYLGK V +AV+TVPAYFND++ + K Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190 Score = 104 bits (249), Expect = 1e-23 Identities = 46/61 (75%), Positives = 53/61 (86%) Frame = +1 Query: 70 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 249 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP NT Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97 Query: 250 I 252 I Sbjct: 98 I 98 Score = 66.5 bits (155), Expect = 3e-12 Identities = 30/33 (90%), Positives = 33/33 (100%) Frame = +2 Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607 QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLD Sbjct: 185 QRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLD 217 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 86.2 bits (204), Expect = 3e-18 Identities = 41/97 (42%), Positives = 60/97 (61%) Frame = +3 Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416 P+ KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166 Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527 +L+KM+ETA YLGK V+NAV+TVPAYFNDS+ + K Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATK 203 Score = 77.4 bits (182), Expect = 2e-15 Identities = 36/65 (55%), Positives = 46/65 (70%), Gaps = 1/65 (1%) Frame = +1 Query: 58 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 234 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 235 NPNNT 249 NP NT Sbjct: 109 NPENT 113 Score = 60.9 bits (141), Expect = 1e-10 Identities = 27/33 (81%), Positives = 31/33 (93%) Frame = +2 Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607 QRQATK AG I+GLNVLR++NEPTAAA+AYGLD Sbjct: 198 QRQATKAAGAIAGLNVLRVVNEPTAAALAYGLD 230 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 55.2 bits (127), Expect = 7e-09 Identities = 23/66 (34%), Positives = 42/66 (63%) Frame = +1 Query: 55 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 234 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 235 NPNNTI 252 N NT+ Sbjct: 63 NFRNTV 68 Score = 43.6 bits (98), Expect = 2e-05 Identities = 26/83 (31%), Positives = 43/83 (51%), Gaps = 1/83 (1%) Frame = +3 Query: 261 KRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 437 KRL GR ++D ++ + ++ G KV Y E+ F ++ + TK+K Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131 Query: 438 TAEAYLGKTVQNAVITVPAYFND 506 AEA L +V + VI++PA+F D Sbjct: 132 IAEAELIGSVSDVVISIPAWFTD 154 Score = 38.7 bits (86), Expect = 7e-04 Identities = 16/33 (48%), Positives = 25/33 (75%) Frame = +2 Query: 506 LQRQATKDAGTISGLNVLRIINEPTAAAIAYGL 604 +QR+A +A I+GLN LR++N+ AAA+ YG+ Sbjct: 155 IQRRALLEAANIAGLNPLRLMNDNAAAALTYGI 187 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 45.6 bits (103), Expect = 6e-06 Identities = 18/61 (29%), Positives = 35/61 (57%) Frame = +1 Query: 70 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNT 249 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N +N+ Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRNADNS 86 Query: 250 I 252 + Sbjct: 87 V 87 Score = 37.1 bits (82), Expect = 0.002 Identities = 17/47 (36%), Positives = 27/47 (57%) Frame = +3 Query: 378 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDK 518 ++K E S L ++ E+AE +LG V V++VP YF D++ K Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 33.1 bits (72), Expect = 0.032 Identities = 27/137 (19%), Positives = 61/137 (44%), Gaps = 11/137 (8%) Frame = -1 Query: 607 VKTVSNRSSSRFIDDSENVQARDGTCIFCGLSLESLKYAGTVITAFCTVLPR*ASAVSFI 428 V ++ + S+S S ++ + + F S S + I++ T+L + S + Sbjct: 576 VSSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSISSSSTILSSPTPSTSSL 635 Query: 427 FVSTMELTSSGKKVLSSPL-----------YATLILGLPPSLTTSKGQCFMSACTVASSN 281 +S+ + S +LSS + Y++ ++ +L +S +S+ VASS+ Sbjct: 636 MISSSSIISGSSSILSSSISTIPISSSLSTYSSSVIPSSSTLVSSSSSLIVSSSPVASSS 695 Query: 280 LRPMRRLASILCCWGSS 230 P+ +S++ + +S Sbjct: 696 SSPIPSSSSLVSTYSAS 712 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 31.1 bits (67), Expect = 0.13 Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Frame = +1 Query: 70 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNN 246 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P + Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPAH 84 Query: 247 TI 252 +I Sbjct: 85 SI 86 Score = 27.5 bits (58), Expect = 1.6 Identities = 11/43 (25%), Positives = 24/43 (55%) Frame = +3 Query: 378 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFND 506 +++ + EE+ +M + AE + + + V+TVP +FN+ Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNE 173 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.70 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +2 Query: 62 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 157 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1087 Score = 25.4 bits (53), Expect = 6.5 Identities = 17/66 (25%), Positives = 31/66 (46%) Frame = +3 Query: 48 QNGKSTRSRNRSGYHVLLRWCLPAREGGDHRQRPGQQDHSVLCCVHRHRASHRRCRQEPG 227 +NGK T +R +S + W P++E R G D + V R + + +EP Sbjct: 477 RNGKKTPTRTKSRMQQFINWFKPSKE----RSSNGNSDSASPPPVPRLSITRSQVSREP- 531 Query: 228 GDEPQQ 245 ++P++ Sbjct: 532 -EKPEE 536 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.4 bits (53), Expect = 6.5 Identities = 11/35 (31%), Positives = 19/35 (54%) Frame = -1 Query: 148 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCI 44 G W SP S + H ++++ +F +RVL C+ Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCM 310 >SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 25.0 bits (52), Expect = 8.6 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = -3 Query: 377 TLICYLDLRFASITDNLERPVLHVGLHSSIFELTSDETFGI 255 T I L + S+TDN+ER + +G + I +TS F + Sbjct: 142 TNIALLLATYPSVTDNIER-FIFMGGSTGIGNITSQAEFNV 181 >SPCC306.05c |ins1||INSIG domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 281 Score = 25.0 bits (52), Expect = 8.6 Identities = 24/73 (32%), Positives = 32/73 (43%), Gaps = 5/73 (6%) Frame = -3 Query: 389 GFVFTLICYLDLRFASITDNLERPVLHVGLHSSIFELTSDETFGINIVLLGFI-----AT 225 GFVFT + I + + P+L V L S FE FG V+LG Sbjct: 104 GFVFTYLA------EQIVQDAKLPLLTVNLKSWKFEPPWPAIFGFVAVILGLSYRRMDTK 157 Query: 224 WFLAASPMRRSVS 186 + L A+P+R S S Sbjct: 158 YPLGAAPLRPSQS 170 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 25.0 bits (52), Expect = 8.6 Identities = 11/38 (28%), Positives = 19/38 (50%) Frame = -1 Query: 538 GTCIFCGLSLESLKYAGTVITAFCTVLPR*ASAVSFIF 425 G C+FCGL+ + T+ C R A+ +S ++ Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864 >SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|||Manual Length = 809 Score = 25.0 bits (52), Expect = 8.6 Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Frame = -1 Query: 604 KTVSNRSSSRFIDDSENVQARDGTCIFC-GLSLESLKY 494 KTV+ R++ F +V +RD +CI C L +S +Y Sbjct: 3 KTVNFRATKNFYLQFVSVSSRDTSCIPCIHLFFDSKRY 40 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 25.0 bits (52), Expect = 8.6 Identities = 11/38 (28%), Positives = 19/38 (50%) Frame = -1 Query: 538 GTCIFCGLSLESLKYAGTVITAFCTVLPR*ASAVSFIF 425 G C+FCGL+ + T+ C R A+ +S ++ Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,646,200 Number of Sequences: 5004 Number of extensions: 56275 Number of successful extensions: 235 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 187 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 232 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 266270664 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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