Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0184
(607 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_8490| Best HMM Match : HSP70 (HMM E-Value=0) 142 3e-34
SB_45540| Best HMM Match : HSP70 (HMM E-Value=0) 139 2e-33
SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.) 136 1e-32
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0) 119 2e-27
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.) 73 1e-13
SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36) 71 6e-13
SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049) 62 4e-10
SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07) 42 4e-04
SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.) 41 9e-04
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 31 0.72
SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.7
SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3) 29 2.2
SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37) 28 5.1
SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9) 28 5.1
SB_50518| Best HMM Match : 7tm_1 (HMM E-Value=7.8e-32) 27 8.9
SB_2033| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 8.9
>SB_8490| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 640
Score = 142 bits (343), Expect = 3e-34
Identities = 66/97 (68%), Positives = 80/97 (82%)
Frame = +3
Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416
P+ DAKRLIGR+++DATVQ+DMK WPF+++SD KPKI+V YKGE KTF EE+SSM
Sbjct: 63 PENTIFDAKRLIGRRYDDATVQSDMKLWPFKIISDNNKPKIQVEYKGERKTFAAEEISSM 122
Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527
VL KMKETAEAYLG+ V +AVITVPAYFNDS+ + K
Sbjct: 123 VLGKMKETAEAYLGQKVTSAVITVPAYFNDSQRQATK 159
Score = 133 bits (321), Expect = 1e-31
Identities = 60/67 (89%), Positives = 64/67 (95%)
Frame = +1
Query: 52 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 231
M KAPA+GIDLGTTYSCVGVFQ+GKVEIIANDQGNRTTPSYV FTDTERL+GDAAKNQVA
Sbjct: 1 MGKAPAIGIDLGTTYSCVGVFQNGKVEIIANDQGNRTTPSYVGFTDTERLVGDAAKNQVA 60
Query: 232 MNPNNTI 252
+NP NTI
Sbjct: 61 LNPENTI 67
Score = 66.5 bits (155), Expect = 2e-11
Identities = 30/33 (90%), Positives = 33/33 (100%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607
QRQATKDAGTI+GLNVLR+INEPTAAA+AYGLD
Sbjct: 154 QRQATKDAGTIAGLNVLRVINEPTAAALAYGLD 186
>SB_45540| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1097
Score = 139 bits (336), Expect = 2e-33
Identities = 63/97 (64%), Positives = 77/97 (79%)
Frame = +3
Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416
P+ DAKRLIGRKF+D V DM HWPF V+ +G +PKI+V +KGE K+FFPEE+SSM
Sbjct: 63 PENTVFDAKRLIGRKFDDPVVARDMTHWPFHVIREGERPKIQVEFKGEKKSFFPEEISSM 122
Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527
VLTKMKETAEAYLG V +AV+TVPAYF+DS+ + K
Sbjct: 123 VLTKMKETAEAYLGAKVTDAVVTVPAYFHDSQRQATK 159
Score = 137 bits (332), Expect = 6e-33
Identities = 63/67 (94%), Positives = 65/67 (97%)
Frame = +1
Query: 52 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 231
MAK PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF+DTERLIGDAAKNQVA
Sbjct: 1 MAKTPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFSDTERLIGDAAKNQVA 60
Query: 232 MNPNNTI 252
MNP NT+
Sbjct: 61 MNPENTV 67
Score = 67.7 bits (158), Expect = 7e-12
Identities = 32/33 (96%), Positives = 33/33 (100%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607
QRQATKDAGTI+GLNVLRIINEPTAAAIAYGLD
Sbjct: 154 QRQATKDAGTIAGLNVLRIINEPTAAAIAYGLD 186
>SB_56180| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 746
Score = 136 bits (329), Expect = 1e-32
Identities = 62/67 (92%), Positives = 64/67 (95%)
Frame = +1
Query: 52 MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 231
M KAPA+GIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTD ERL+GDAAKNQVA
Sbjct: 1 MTKAPAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDEERLVGDAAKNQVA 60
Query: 232 MNPNNTI 252
MNP NTI
Sbjct: 61 MNPTNTI 67
Score = 116 bits (280), Expect = 1e-26
Identities = 66/122 (54%), Positives = 77/122 (63%), Gaps = 25/122 (20%)
Frame = +3
Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEV--- 407
P DAKRLIGR+F+D V+ DMKHW FEVV++ G+PK+KV YKGE KTFF EEV
Sbjct: 63 PTNTIFDAKRLIGRRFDDPGVKDDMKHWSFEVVNEAGRPKVKVEYKGETKTFFAEEVVNE 122
Query: 408 ----------------------SSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKP 521
SSMVL KMKETAEAYLG V +AV+TVPAYFNDS+ +
Sbjct: 123 AGRPKVKVEYKGETKTFFAEEISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQA 182
Query: 522 QK 527
K
Sbjct: 183 TK 184
Score = 66.9 bits (156), Expect = 1e-11
Identities = 32/33 (96%), Positives = 32/33 (96%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607
QRQATKDAG ISGLNVLRIINEPTAAAIAYGLD
Sbjct: 247 QRQATKDAGVISGLNVLRIINEPTAAAIAYGLD 279
Score = 62.1 bits (144), Expect = 3e-10
Identities = 29/42 (69%), Positives = 35/42 (83%)
Frame = +3
Query: 402 EVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527
++SSMVL KMKETAEAYLG V +AV+TVPAYFNDS+ + K
Sbjct: 211 QISSMVLNKMKETAEAYLGCKVTDAVVTVPAYFNDSQRQATK 252
>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
Length = 1327
Score = 119 bits (287), Expect = 2e-27
Identities = 57/97 (58%), Positives = 73/97 (75%)
Frame = +3
Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSM 416
P+ DAKRLIGR ++D +VQ D+K +PF+VV KP ++V K E KTF EE+S+M
Sbjct: 761 PENTVFDAKRLIGRSWDDTSVQNDLKFFPFKVVDKNKKPHVQVKVKDEIKTFAAEEISAM 820
Query: 417 VLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527
VLTKMKETAEAYLGK V +AV+TVPAYFND++ + K
Sbjct: 821 VLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATK 857
Score = 105 bits (252), Expect = 3e-23
Identities = 48/62 (77%), Positives = 55/62 (88%), Gaps = 1/62 (1%)
Frame = +1
Query: 70 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDT-ERLIGDAAKNQVAMNPNN 246
+GIDLGTTYSCVGVF++G+VEIIANDQGNR TPSYVAFT+ ERLIGD AKNQ+ NP N
Sbjct: 704 IGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTEEGERLIGDGAKNQLTTNPEN 763
Query: 247 TI 252
T+
Sbjct: 764 TV 765
Score = 61.7 bits (143), Expect = 4e-10
Identities = 28/33 (84%), Positives = 31/33 (93%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGLD 607
QRQATKDAG I+GL V+RIINEPTAAAIAYG+D
Sbjct: 852 QRQATKDAGAIAGLTVMRIINEPTAAAIAYGMD 884
>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1068
Score = 73.3 bits (172), Expect = 1e-13
Identities = 36/83 (43%), Positives = 52/83 (62%), Gaps = 1/83 (1%)
Frame = +3
Query: 261 KRLIGRKFEDATVQADMKHWPFEVVS-DGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 437
KR IGRKF D VQ ++ H P++VV I+V Y G+ + F PE+V +M+ T++K
Sbjct: 127 KRFIGRKFSDPAVQKEIPHLPYKVVELPNDSIGIQVQYLGKQEVFSPEQVMAMLFTRLKT 186
Query: 438 TAEAYLGKTVQNAVITVPAYFND 506
TAE L V + VI+VP+Y+ D
Sbjct: 187 TAEIALKTKVTDCVISVPSYYTD 209
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/52 (42%), Positives = 33/52 (63%)
Frame = +1
Query: 70 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 225
VG D+G + V + G +E +AN+ +R TPS+V+ D +RLIG + KNQ
Sbjct: 4 VGFDVGNQSCYIAVARGGGIETVANEFSDRCTPSFVSLGDKQRLIGTSGKNQ 55
Score = 37.5 bits (83), Expect = 0.008
Identities = 15/32 (46%), Positives = 23/32 (71%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGL 604
QR+ DA +GLN LR++N+ TA ++AYG+
Sbjct: 211 QRRCMLDASATAGLNCLRLMNDTTAVSLAYGI 242
>SB_51590| Best HMM Match : HSP70 (HMM E-Value=4.4e-36)
Length = 437
Score = 71.3 bits (167), Expect = 6e-13
Identities = 32/67 (47%), Positives = 46/67 (68%), Gaps = 2/67 (2%)
Frame = +1
Query: 58 KAPAVGIDLGTTYSCVGVFQ--HGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 231
K +GIDLGTTYSCVG +Q G+++I +++ ++ PS VAFTD L+G+ A +Q
Sbjct: 30 KPKIIGIDLGTTYSCVGAYQAISGRIDIFSDENDDKVVPSLVAFTDKGVLVGEKALSQAE 89
Query: 232 MNPNNTI 252
+NP NTI
Sbjct: 90 INPKNTI 96
Score = 54.8 bits (126), Expect = 5e-08
Identities = 37/105 (35%), Positives = 51/105 (48%), Gaps = 1/105 (0%)
Frame = +3
Query: 237 PQQHNIDAKRLIGRKFEDATVQADMKHWPFEVVSDG-GKPKIKVAYKGEDKTFFPEEVSS 413
P+ DAKR IG+ F + K + F V +D G V KG PEE+
Sbjct: 92 PKNTIYDAKRFIGKTFTKQELLEIGKLYQFRVTTDKRGNAGFLVGKKGNFTVITPEEIGG 151
Query: 414 MVLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQKMQVPSLA 548
V+ K++ AE L TV AV++VPA F D K + ++ SLA
Sbjct: 152 YVIKKLRTMAERNLTTTVTKAVMSVPAEF-DIKQRNATVKAASLA 195
Score = 48.4 bits (110), Expect = 4e-06
Identities = 21/32 (65%), Positives = 27/32 (84%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGL 604
QR AT A +++G+ VLR+INEPTAAA+AYGL
Sbjct: 184 QRNATVKAASLAGITVLRVINEPTAAALAYGL 215
>SB_57217| Best HMM Match : Helicase_C (HMM E-Value=0.049)
Length = 179
Score = 61.7 bits (143), Expect = 4e-10
Identities = 28/57 (49%), Positives = 43/57 (75%)
Frame = +3
Query: 357 IKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSKDKPQK 527
I V GE ++F PE++S++VL ++K AEA+LG+ V+ AVITVPA+FND++ + K
Sbjct: 2 IAVEVCGEKRSFAPEQISAVVLEQLKADAEAFLGQAVKRAVITVPAHFNDAQRQATK 58
Score = 42.7 bits (96), Expect = 2e-04
Identities = 31/57 (54%), Positives = 33/57 (57%), Gaps = 24/57 (42%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVL------------------------RIINEPTAAAIAYGLD 607
QRQATKDAGTI+GL+VL RIINEPTAAAIAYGLD
Sbjct: 53 QRQATKDAGTIAGLDVLSRTASERPPSRFRRRRPHGRSRVLRIINEPTAAAIAYGLD 109
>SB_47391| Best HMM Match : HSP70 (HMM E-Value=2.7e-07)
Length = 592
Score = 41.9 bits (94), Expect = 4e-04
Identities = 26/61 (42%), Positives = 34/61 (55%), Gaps = 3/61 (4%)
Frame = +1
Query: 121 GKVEIIANDQGNRTTPSYVAFTDTERLI--GDAAKNQVAMNPNNTILMP-NVSSDVSSKM 291
G IIAND G+R TP+ VAF+DTE+LI +V N N + P V + + KM
Sbjct: 83 GNTTIIANDAGDRVTPAMVAFSDTEKLIDKDGLPYYEVESNERNIQVSPKEVINMIYKKM 142
Query: 292 L 294
L
Sbjct: 143 L 143
>SB_31398| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1019
Score = 40.7 bits (91), Expect = 9e-04
Identities = 18/54 (33%), Positives = 31/54 (57%)
Frame = +3
Query: 351 PKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSK 512
P +K Y+ D TF PEE+ M+L + E + +++ V+TVP +FN ++
Sbjct: 68 PHVK-NYQQSDVTFTPEELMGMILNHSRFIGEQFADHPIKDVVLTVPPFFNQAE 120
Score = 33.9 bits (74), Expect = 0.10
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = +2
Query: 509 QRQATKDAGTISGLNVLRIINEPTAAAIAYGL 604
+R+A A + GLNVL+I+N TA A+ YGL
Sbjct: 120 ERRALLRAAELVGLNVLQIMNSNTAVALNYGL 151
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 31.1 bits (67), Expect = 0.72
Identities = 15/25 (60%), Positives = 20/25 (80%), Gaps = 1/25 (4%)
Frame = +1
Query: 130 EIIANDQGNRTTPSYVAFT-DTERL 201
++I N +G+RTTPS VAFT + ERL
Sbjct: 7 KVIENAEGSRTTPSVVAFTPEGERL 31
>SB_24848| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1506
Score = 29.9 bits (64), Expect = 1.7
Identities = 19/66 (28%), Positives = 31/66 (46%)
Frame = -1
Query: 445 SAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSACTVASSNLRPMR 266
S F+F ST + +S +KV+ SPL + + P+ G S C V + + P
Sbjct: 1108 SPAKFVFTSTGIVRASTRKVVPSPLRRSKVPPTVPAQRLFNGDRVESICNVQNLSRCPKI 1167
Query: 265 RLASIL 248
A++L
Sbjct: 1168 SNATVL 1173
>SB_7560| Best HMM Match : Metallothio_PEC (HMM E-Value=1.3)
Length = 350
Score = 29.5 bits (63), Expect = 2.2
Identities = 16/33 (48%), Positives = 19/33 (57%)
Frame = -1
Query: 238 GSSPPGSWRHLR*DARCL*TQHKTEWSCCPGRW 140
GS PPG W R +AR T +T +SC PG W
Sbjct: 206 GSCPPGFWCRKRREARAA-TDLET-YSCPPGFW 236
>SB_19079| Best HMM Match : LCM (HMM E-Value=6.3e-37)
Length = 420
Score = 28.3 bits (60), Expect = 5.1
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -1
Query: 259 ASILCCWGSSPPGSWRH 209
A ++CC G SP WRH
Sbjct: 394 ARVVCCQGDSPSPRWRH 410
>SB_58645| Best HMM Match : Kelch_1 (HMM E-Value=1.9)
Length = 340
Score = 28.3 bits (60), Expect = 5.1
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = -1
Query: 259 ASILCCWGSSPPGSWRH 209
A ++CC G SP WRH
Sbjct: 169 ARVVCCQGDSPSPRWRH 185
>SB_50518| Best HMM Match : 7tm_1 (HMM E-Value=7.8e-32)
Length = 375
Score = 27.5 bits (58), Expect = 8.9
Identities = 15/44 (34%), Positives = 19/44 (43%), Gaps = 7/44 (15%)
Frame = +3
Query: 168 VLCCVHRHRASHRRCRQEPGGDEPQQ-------HNIDAKRLIGR 278
+LCC R + RRCR E HN+ AK L+ R
Sbjct: 308 ILCCGKRSKEGKRRCRYLTTSSESSDRQTNMSGHNMSAKELLSR 351
>SB_2033| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 995
Score = 27.5 bits (58), Expect = 8.9
Identities = 13/56 (23%), Positives = 27/56 (48%)
Frame = +1
Query: 115 QHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPNNTILMPNVSSDVS 282
+ G+ ++I TP Y + D + D + +++ + PN ++L+ NV S
Sbjct: 171 ERGQSQVIRCSAEGYPTPHYQWYKDWNPTVLDYSDDRLTLLPNGSLLISNVQESDS 226
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,715,509
Number of Sequences: 59808
Number of extensions: 471917
Number of successful extensions: 1411
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1304
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1407
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1475788250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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