Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0166
(674 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q16822 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 124 3e-27
UniRef50_Q9HLV2 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 95 2e-18
UniRef50_Q8FM16 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 93 7e-18
UniRef50_UPI000038E5C0 Cluster: hypothetical protein Faci_030001... 89 7e-17
UniRef50_A7HGY6 Cluster: Phosphoenolpyruvate carboxykinase; n=16... 88 2e-16
UniRef50_A1SQ84 Cluster: Phosphoenolpyruvate carboxykinase; n=2;... 82 1e-14
UniRef50_Q5MGN2 Cluster: Putative uncharacterized protein; n=1; ... 81 2e-14
UniRef50_Q4J9S8 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 49 1e-04
UniRef50_Q8U410 Cluster: Phosphoenolpyruvate carboxykinase [GTP]... 44 0.004
UniRef50_Q9TYQ8 Cluster: Putative uncharacterized protein; n=2; ... 40 0.073
UniRef50_A1S0E8 Cluster: Phosphoenolpyruvate carboxykinase; n=2;... 39 0.13
UniRef50_Q7SAF0 Cluster: Predicted protein; n=1; Neurospora cras... 35 2.1
UniRef50_A5NU87 Cluster: Transcriptional regulator, Fis family p... 34 2.7
UniRef50_P91756 Cluster: Phosphoenolpyruvate carboxykinase; n=1;... 34 3.6
UniRef50_UPI0000498CBE Cluster: hypothetical protein 114.t00026;... 33 6.3
UniRef50_A7RRG1 Cluster: Predicted protein; n=1; Nematostella ve... 33 6.3
UniRef50_A6RST8 Cluster: Putative uncharacterized protein; n=1; ... 33 6.3
UniRef50_Q9FF35 Cluster: Similarity to protein kinase; n=1; Arab... 33 8.4
UniRef50_Q3E8U5 Cluster: Uncharacterized protein At5g29090.1; n=... 33 8.4
UniRef50_Q00WG4 Cluster: Chromosome 14 contig 1, DNA sequence; n... 33 8.4
>UniRef50_Q16822 Cluster: Phosphoenolpyruvate carboxykinase [GTP],
mitochondrial precursor; n=571; cellular organisms|Rep:
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
precursor - Homo sapiens (Human)
Length = 640
Score = 124 bits (298), Expect = 3e-27
Identities = 53/92 (57%), Positives = 68/92 (73%), Gaps = 1/92 (1%)
Frame = +3
Query: 3 YLKHWLSMP-QPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAE 179
YL+HWLSM + G ++P+IFHVNWFR+DE G FLWPGFGEN+RVLDWI RR +GE E
Sbjct: 511 YLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARE 570
Query: 180 TPLGYIPRAGALNTENLSAVDMNKLSAYLKTF 275
TP+G +P+ GAL+ L A+D +L + K F
Sbjct: 571 TPIGLVPKEGALDLSGLRAIDTTQLFSLPKDF 602
Score = 45.6 bits (103), Expect = 0.001
Identities = 20/44 (45%), Positives = 25/44 (56%)
Frame = +2
Query: 248 QAVSIPKDFWLQEADAIXKYFKEEVGEDLPNEMWDELNKLRNNI 379
Q S+PKDFW QE I Y E+V +DLP E+ EL L +
Sbjct: 594 QLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRV 637
>UniRef50_Q9HLV2 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=3; Thermoplasma|Rep: Phosphoenolpyruvate carboxykinase
[GTP] - Thermoplasma acidophilum
Length = 588
Score = 94.7 bits (225), Expect = 2e-18
Identities = 40/84 (47%), Positives = 54/84 (64%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAET 182
Y +HW+ M + + PKIF+VNWFR+ + G F+WPGF EN RV++WIL R D ET
Sbjct: 464 YFRHWIEMGRKLSRRPKIFYVNWFRRRQDGSFIWPGFSENFRVIEWILYRLDHNDNAIET 523
Query: 183 PLGYIPRAGALNTENLSAVDMNKL 254
P+GYIP + NL+ DM +L
Sbjct: 524 PIGYIPENINTDGLNLTKQDMEEL 547
>UniRef50_Q8FM16 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=116; Bacteria|Rep: Phosphoenolpyruvate carboxykinase
[GTP] - Corynebacterium efficiens
Length = 612
Score = 92.7 bits (220), Expect = 7e-18
Identities = 42/77 (54%), Positives = 52/77 (67%), Gaps = 1/77 (1%)
Frame = +3
Query: 3 YLKHWLSMPQPGR-KMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAE 179
YL++W+ M G KMP IF VNWFR+ E G+FLWPGFGENSRVL W++ R +G E
Sbjct: 479 YLQNWIDMGNKGGDKMPSIFLVNWFRRGEDGRFLWPGFGENSRVLKWVIDRIEGRVGAEE 538
Query: 180 TPLGYIPRAGALNTENL 230
T +GY RA L+ E L
Sbjct: 539 TVVGYTARAEDLDLEGL 555
>UniRef50_UPI000038E5C0 Cluster: hypothetical protein Faci_03000127;
n=1; Ferroplasma acidarmanus fer1|Rep: hypothetical
protein Faci_03000127 - Ferroplasma acidarmanus fer1
Length = 598
Score = 89.4 bits (212), Expect = 7e-17
Identities = 38/84 (45%), Positives = 51/84 (60%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAET 182
Y +HWL M + + P+IF+VNWFRKD G F+WPGF EN RV++WI R D + ET
Sbjct: 470 YFQHWLDMGKLVQHRPEIFYVNWFRKDSDGNFIWPGFSENFRVIEWIASRLDAKANAIET 529
Query: 183 PLGYIPRAGALNTENLSAVDMNKL 254
P+G IP + NL + +L
Sbjct: 530 PVGLIPDIANFASGNLGKNKLEQL 553
Score = 37.9 bits (84), Expect = 0.22
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = +2
Query: 245 EQAVSIPKDFWLQEADAIXKYFKEEVGEDLPNEMWDELNKLRNNIQGQ 388
EQ I WL+E + I +FK+ +G+ P E+W E + +++ ++ Q
Sbjct: 551 EQLFEIDYPGWLKELNEIEPFFKK-IGDSFPEELWREFHNMKDGVEKQ 597
>UniRef50_A7HGY6 Cluster: Phosphoenolpyruvate carboxykinase; n=16;
cellular organisms|Rep: Phosphoenolpyruvate
carboxykinase - Anaeromyxobacter sp. Fw109-5
Length = 595
Score = 88.2 bits (209), Expect = 2e-16
Identities = 38/78 (48%), Positives = 48/78 (61%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAET 182
Y +HWL M PK++ VNWFRK + GKFLWPG+G+N RVL W+L R G ET
Sbjct: 462 YFQHWLDMQSRIPNPPKVYMVNWFRKSDDGKFLWPGYGDNMRVLKWMLDRAAGRVGAQET 521
Query: 183 PLGYIPRAGALNTENLSA 236
LG P+AG L+ + A
Sbjct: 522 LLGNTPKAGDLDLSGIDA 539
>UniRef50_A1SQ84 Cluster: Phosphoenolpyruvate carboxykinase; n=2;
Nocardioides sp. JS614|Rep: Phosphoenolpyruvate
carboxykinase - Nocardioides sp. (strain BAA-499 /
JS614)
Length = 617
Score = 82.2 bits (194), Expect = 1e-14
Identities = 35/88 (39%), Positives = 51/88 (57%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAET 182
Y +HWL++ R P HVNWF++ + G+FLWPG+ EN R L W+++ +GE ET
Sbjct: 486 YAQHWLNVIAGARNKPIFAHVNWFQRGDDGRFLWPGYRENLRPLVWLMQLKNGEVTGVET 545
Query: 183 PLGYIPRAGALNTENLSAVDMNKLSAYL 266
P+G +P LN + L + L A L
Sbjct: 546 PVGILPSRDELNLDGLDEQTLADLDAVL 573
>UniRef50_Q5MGN2 Cluster: Putative uncharacterized protein; n=1;
Lonomia obliqua|Rep: Putative uncharacterized protein -
Lonomia obliqua (Moth)
Length = 53
Score = 81.0 bits (191), Expect = 2e-14
Identities = 35/41 (85%), Positives = 37/41 (90%)
Frame = +2
Query: 257 SIPKDFWLQEADAIXKYFKEEVGEDLPNEMWDELNKLRNNI 379
SIPKDFWLQE+DA KYFKEEVGEDLP EMW+ELNKLR NI
Sbjct: 10 SIPKDFWLQESDAFEKYFKEEVGEDLPVEMWEELNKLRKNI 50
>UniRef50_Q4J9S8 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=4; Sulfolobaceae|Rep: Phosphoenolpyruvate
carboxykinase [GTP] - Sulfolobus acidocaldarius
Length = 604
Score = 48.8 bits (111), Expect = 1e-04
Identities = 26/67 (38%), Positives = 40/67 (59%), Gaps = 1/67 (1%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRV-LDWILRRCDGEPCHAE 179
YL ++L+ + + +PKIF N+F KDE KFL E+ RV + W ++R + E
Sbjct: 456 YLNNYLTFGRNLKYVPKIFSFNYFLKDENNKFL--NSKEDKRVWVKWAVKRVESETDAIY 513
Query: 180 TPLGYIP 200
TP+G+IP
Sbjct: 514 TPVGFIP 520
>UniRef50_Q8U410 Cluster: Phosphoenolpyruvate carboxykinase [GTP];
n=6; cellular organisms|Rep: Phosphoenolpyruvate
carboxykinase [GTP] - Pyrococcus furiosus
Length = 624
Score = 43.6 bits (98), Expect = 0.004
Identities = 25/67 (37%), Positives = 39/67 (58%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAET 182
YL+++L + +K PKIF VN+F + E G++L + + L W+ R + ET
Sbjct: 478 YLRNYLEFGRKLKKTPKIFAVNYFLR-ENGQWLNEKL-DKAVWLKWMELRVHNDVGAIET 535
Query: 183 PLGYIPR 203
P+GYIPR
Sbjct: 536 PIGYIPR 542
>UniRef50_Q9TYQ8 Cluster: Putative uncharacterized protein; n=2;
Caenorhabditis|Rep: Putative uncharacterized protein -
Caenorhabditis elegans
Length = 624
Score = 39.5 bits (88), Expect = 0.073
Identities = 25/79 (31%), Positives = 41/79 (51%), Gaps = 12/79 (15%)
Frame = +3
Query: 3 YLKHWLSMPQPGRKM---------PKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRC 155
Y+KHWL M + P+IF N +++ + GK LWPG +N+R+ ++I RC
Sbjct: 492 YVKHWLEMGVGVKSSSENFENPPPPQIFFTNLYQEVD-GKPLWPGGVDNARIFEYIYERC 550
Query: 156 DGEPCHAET---PLGYIPR 203
++T LG +P+
Sbjct: 551 ANPADLSKTISSGLGIVPK 569
>UniRef50_A1S0E8 Cluster: Phosphoenolpyruvate carboxykinase; n=2;
Thermoprotei|Rep: Phosphoenolpyruvate carboxykinase -
Thermofilum pendens (strain Hrk 5)
Length = 636
Score = 38.7 bits (86), Expect = 0.13
Identities = 20/54 (37%), Positives = 30/54 (55%)
Frame = +3
Query: 39 RKMPKIFHVNWFRKDEQGKFLWPGFGENSRVLDWILRRCDGEPCHAETPLGYIP 200
R PKIF VN+F + + G +L + L W+ R +GE +TP+GY+P
Sbjct: 500 RVTPKIFGVNYFLRGKDGNYLAEK-RDKLAWLRWMEARVNGEVGAVKTPVGYVP 552
>UniRef50_Q7SAF0 Cluster: Predicted protein; n=1; Neurospora
crassa|Rep: Predicted protein - Neurospora crassa
Length = 589
Score = 34.7 bits (76), Expect = 2.1
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = -3
Query: 570 GDLEVDPRQVEGRSAEGQKGNNVADEDVEDRAKWGSRIGKAGPT 439
G+LEV PR EG G KG+ V+D + + W GK PT
Sbjct: 175 GNLEVHPRNREGSLRGGNKGSEVSDRAQKQQPSWVPP-GKTSPT 217
>UniRef50_A5NU87 Cluster: Transcriptional regulator, Fis family
precursor; n=1; Methylobacterium sp. 4-46|Rep:
Transcriptional regulator, Fis family precursor -
Methylobacterium sp. 4-46
Length = 782
Score = 34.3 bits (75), Expect = 2.7
Identities = 19/40 (47%), Positives = 21/40 (52%)
Frame = -3
Query: 207 RLSVCTPVESRRGRAPHRSDVRSSLRPESSRRNRARGTYP 88
R S T RRGRA RSD R+S RP R R R +P
Sbjct: 124 RTSARTSARERRGRARERSDERTSFRPIDFAR-RVRSAFP 162
>UniRef50_P91756 Cluster: Phosphoenolpyruvate carboxykinase; n=1;
Lumbricus rubellus|Rep: Phosphoenolpyruvate
carboxykinase - Lumbricus rubellus (Humus earthworm)
Length = 71
Score = 33.9 bits (74), Expect = 3.6
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = +2
Query: 248 QAVSIPKDFWLQEADAIXKYFKEEVGEDLPNEMWDELNK 364
Q ++PK +W ++ ++ KE+VG DLP + +L++
Sbjct: 25 QMFALPKAYWTEDIAETKRFLKEQVGSDLPEAIRRQLDE 63
>UniRef50_UPI0000498CBE Cluster: hypothetical protein 114.t00026;
n=1; Entamoeba histolytica HM-1:IMSS|Rep: hypothetical
protein 114.t00026 - Entamoeba histolytica HM-1:IMSS
Length = 751
Score = 33.1 bits (72), Expect = 6.3
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -3
Query: 369 LNLFSSSHISLGRSSPTSSLKYFXMASASCSQKSLGMLTACSCRQRLGFPYSEHRLSVCT 190
L F S I++ S+ S + +C +K + L + + RQR+ FPYS + +
Sbjct: 86 LGCFFSGIINIDSSASNLSRTRSPLPLVNCIEKKVSELKSSTSRQRIEFPYSHQKSKIEA 145
Query: 189 P 187
P
Sbjct: 146 P 146
>UniRef50_A7RRG1 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 1024
Score = 33.1 bits (72), Expect = 6.3
Identities = 15/42 (35%), Positives = 27/42 (64%)
Frame = -2
Query: 163 SPSQRRKIQSKTREFSPKPGQRNLPCSSLRNQLTWKILGILR 38
+P+Q+++++SK R SP PG NLP N ++++G+ R
Sbjct: 790 TPNQKQRLKSKRRVDSPTPGAENLPVFRTPN---FRLVGVAR 828
>UniRef50_A6RST8 Cluster: Putative uncharacterized protein; n=1;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 4513
Score = 33.1 bits (72), Expect = 6.3
Identities = 25/94 (26%), Positives = 39/94 (41%)
Frame = -2
Query: 307 VLXDGVSLLQPKVFRYADSLFMSTALRFSVFRAPALGMYPSGVSAWQGSPSQRRKIQSKT 128
VL D V+LL V R A + AL F+ P G Y S + + ++
Sbjct: 2258 VLPDSVALLHQYVERGAKTWPDKVALEFATCLQP--GNYQSQKWTYLQLDEESNRVAQML 2315
Query: 127 REFSPKPGQRNLPCSSLRNQLTWKILGILRPGCG 26
PG+ C + ++ I+GI++ GCG
Sbjct: 2316 HARGTTPGEIIAVCFDKCAEASFAIIGIMKAGCG 2349
>UniRef50_Q9FF35 Cluster: Similarity to protein kinase; n=1;
Arabidopsis thaliana|Rep: Similarity to protein kinase -
Arabidopsis thaliana (Mouse-ear cress)
Length = 978
Score = 32.7 bits (71), Expect = 8.4
Identities = 18/49 (36%), Positives = 23/49 (46%)
Frame = +3
Query: 93 KFLWPGFGENSRVLDWILRRCDGEPCHAETPLGYIPRAGALNTENLSAV 239
KF+ P G S D + G + P+GY P GALN NL +V
Sbjct: 149 KFICPNKGVGSIHQDDLYHNHCGGIFNITVPIGYAPEEGALNVTNLESV 197
>UniRef50_Q3E8U5 Cluster: Uncharacterized protein At5g29090.1; n=1;
Arabidopsis thaliana|Rep: Uncharacterized protein
At5g29090.1 - Arabidopsis thaliana (Mouse-ear cress)
Length = 195
Score = 32.7 bits (71), Expect = 8.4
Identities = 17/38 (44%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = +3
Query: 102 WPGFGENSRVLDWILRRCDGEPCHA--ETPLGYIPRAG 209
W G+ N RVLD + RCDG+ H E+ G I R G
Sbjct: 54 WSGWASNVRVLDGVAGRCDGQNGHVLNESIGGPIGRGG 91
>UniRef50_Q00WG4 Cluster: Chromosome 14 contig 1, DNA sequence; n=1;
Ostreococcus tauri|Rep: Chromosome 14 contig 1, DNA
sequence - Ostreococcus tauri
Length = 350
Score = 32.7 bits (71), Expect = 8.4
Identities = 18/39 (46%), Positives = 20/39 (51%)
Frame = -3
Query: 216 SEHRLSVCTPVESRRGRAPHRSDVRSSLRPESSRRNRAR 100
S H V +P RGR R V SS RP + RR RAR
Sbjct: 286 SAHPTRVPSPPTRARGRRRGRDTVASSRRPRTPRRRRAR 324
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 680,638,065
Number of Sequences: 1657284
Number of extensions: 14152430
Number of successful extensions: 44738
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 42886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 44700
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 52066120554
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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