Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0149
(677 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g76150.1 68414.m08843 maoC-like dehydratase domain-containing... 58 4e-09
At5g60340.1 68418.m07564 maoC-like dehydratase domain-containing... 35 0.043
At1g49910.1 68414.m05597 WD-40 repeat family protein / mitotic c... 32 0.40
At5g54570.1 68418.m06793 glycosyl hydrolase family 1 protein con... 29 2.8
At5g11070.1 68418.m01293 expressed protein 29 2.8
At1g12000.1 68414.m01386 pyrophosphate--fructose-6-phosphate 1-p... 29 2.8
At4g26990.1 68417.m03883 expressed protein 29 3.7
At1g50950.1 68414.m05728 thioredoxin-related contains weak hit t... 29 3.7
At1g65610.1 68414.m07442 endo-1,4-beta-glucanase, putative / cel... 28 4.9
At1g63530.1 68414.m07182 hypothetical protein 28 6.5
At5g57740.1 68418.m07218 zinc finger (C3HC4-type RING finger) fa... 27 8.6
At4g03380.1 68417.m00460 expressed protein 27 8.6
At3g16680.1 68416.m02131 expressed protein ; expression supporte... 27 8.6
At3g09650.1 68416.m01144 pentatricopeptide (PPR) repeat-containi... 27 8.6
At2g45460.1 68415.m05654 forkhead-associated domain-containing p... 27 8.6
At2g44030.1 68415.m05474 kelch repeat-containing F-box family pr... 27 8.6
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ... 27 8.6
>At1g76150.1 68414.m08843 maoC-like dehydratase domain-containing
protein contains similarity to Swiss-Prot:P51659
estradiol 17 beta-dehydrogenase 4 (17-beta-HSD 4)
(17-beta-hydroxysteroid dehydrogenase 4) [Homo sapiens];
contains Pfam profile PF01575: MaoC like domain
Length = 309
Score = 58.4 bits (135), Expect = 4e-09
Identities = 26/54 (48%), Positives = 35/54 (64%)
Frame = +1
Query: 511 LYRMSGDLNPLHIDPNVATASGHKKPILHGLASLGFSARHVLAKFGGNDSSNVK 672
LYR+SGD NPLH DP A +G +PILHGL +LGF+ + ++ D + VK
Sbjct: 202 LYRLSGDYNPLHSDPEFAKLAGFPRPILHGLCTLGFAIKAIIKCVCKGDPTAVK 255
Score = 47.6 bits (108), Expect = 8e-06
Identities = 31/89 (34%), Positives = 45/89 (50%), Gaps = 5/89 (5%)
Frame = +3
Query: 6 YDYNAKDLVIYALGVGASVENPTD---LKFLY--ESHENFSPLPSFFILAGMCMEAPIVA 170
Y YN +D+ IYALG+GA ++ D LKF+Y + LP+F A + +
Sbjct: 23 YTYNERDVAIYALGIGACGQDAVDSDELKFVYHRNGQDLIQVLPTF---ASLFTLGSLTE 79
Query: 171 NAMPPGKFADFTNVLHGEQYIEFVGDFPA 257
PG D + +LHG+QYIE P+
Sbjct: 80 GLDLPGFKYDPSLLLHGQQYIEIYRPLPS 108
Score = 35.9 bits (79), Expect = 0.025
Identities = 30/101 (29%), Positives = 50/101 (49%), Gaps = 9/101 (8%)
Frame = +2
Query: 236 IRR*LPCTEGVFTTRCYVVDILDKGSSAVAIVNSEIFQ--NKQLVCRTQQHIFVLGQGGF 409
I R LP ++ + + + DKG +A+ + + ++ + +L+C + +F+ G GGF
Sbjct: 102 IYRPLP-SKASLINKVSLAGLQDKGKAAILELETRSYEEGSGELLCMNRTTVFLRGAGGF 160
Query: 410 GG---PRNSKN-AIGVGNA---PKRNPDAVVEQRTAEDQAL 511
P + KN G A P+R P V E+RT QAL
Sbjct: 161 SNSSQPFSYKNYPSNQGLAVKIPQRQPLTVCEERTQPSQAL 201
>At5g60340.1 68418.m07564 maoC-like dehydratase domain-containing
protein contains similarity to (R)-specific enoyl-CoA
hydratase PhaJ1 [Pseudomonas oleovorans]
gi|22506675|gb|AAM97601; contains Pfam domain PF01575:
MaoC like domain
Length = 337
Score = 35.1 bits (77), Expect = 0.043
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +1
Query: 520 MSGDLNPLHIDPNVATASGHKKPILHGLASLGFSARHVLAKFGG 651
+S D NPLH DP A +G + ++HG+ R + A F G
Sbjct: 220 VSHDWNPLHFDPESARKAGFENRLVHGMLVSSMFPRIISAHFPG 263
>At1g49910.1 68414.m05597 WD-40 repeat family protein / mitotic
checkpoint protein, putative contains 5 WD-40 repeats
(PF00400) (1 weak); similar to testis mitotic checkpoint
protein BUB3 (GB:AAC28439,SP:O43684)[Homo sapiens]
Length = 339
Score = 31.9 bits (69), Expect = 0.40
Identities = 21/71 (29%), Positives = 33/71 (46%), Gaps = 4/71 (5%)
Frame = -3
Query: 663 AGVVSTKFS*NMSS*KTKGCESMENRLLVSTCGRNVGI----NMQRIEVTRHSVERAWSS 496
+G T+ M + + NRL+V+T GR+V I NM + E R S + +
Sbjct: 130 SGTERTQIGTYMQPERVNSLSLVGNRLVVATAGRHVNIYDLRNMSQPEQRRESSLKYQTR 189
Query: 495 AVRCSTTASGF 463
VRC +G+
Sbjct: 190 CVRCYPNGTGY 200
>At5g54570.1 68418.m06793 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to amygdalin hydrolase isoform AH I precursor
(GI:16757966) [Prunus serotina]
Length = 535
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
Frame = +3
Query: 183 PGKFADFTNV-LHGEQYIEFVGDFPAPRESSQPVVTLSISWTR 308
PGK DF+N +QY F D ++ SISW+R
Sbjct: 72 PGKILDFSNADTTVDQYHRFHNDIDLMKDLRMDAYRFSISWSR 114
>At5g11070.1 68418.m01293 expressed protein
Length = 152
Score = 29.1 bits (62), Expect = 2.8
Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 1/47 (2%)
Frame = +1
Query: 121 PRSSY-WLECAWRPRSLLTPCRRANSLISLMCFTESSTLNSSVTSLH 258
PRS Y WL+ + L CRR S I + C + N+ V H
Sbjct: 49 PRSPYEWLKSTAQELELRDRCRRVKSRIKVTCRNNNCAYNNCVHHHH 95
>At1g12000.1 68414.m01386 pyrophosphate--fructose-6-phosphate
1-phosphotransferase beta subunit, putative /
pyrophosphate-dependent 6-phosphofructose-1-kinase,
putative strong similarity to SP|Q41141
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
beta subunit (EC 2.7.1.90) (PFP) ((PPI-PFK) {Ricinus
communis}; contains Pfam profile PF00365:
Phosphofructokinase
Length = 566
Score = 29.1 bits (62), Expect = 2.8
Identities = 20/70 (28%), Positives = 32/70 (45%)
Frame = +3
Query: 105 NFSPLPSFFILAGMCMEAPIVANAMPPGKFADFTNVLHGEQYIEFVGDFPAPRESSQPVV 284
N PLPS A +E P + A P + A L+G+ + V D AP + + +
Sbjct: 39 NTLPLPSVLKGAFKIVEGPASSAAGNPDEIAKLFPGLYGQPSVAVVPDQDAPSSAPKLKI 98
Query: 285 TLSISWTRAP 314
+ +S +AP
Sbjct: 99 GVVLSGGQAP 108
>At4g26990.1 68417.m03883 expressed protein
Length = 474
Score = 28.7 bits (61), Expect = 3.7
Identities = 16/42 (38%), Positives = 19/42 (45%)
Frame = +3
Query: 159 PIVANAMPPGKFADFTNVLHGEQYIEFVGDFPAPRESSQPVV 284
P V A+PP KF + NV G VG F P+ P V
Sbjct: 306 PYVPQALPPSKFVPYGNVTAGHA----VGGFQFPQHMIGPTV 343
>At1g50950.1 68414.m05728 thioredoxin-related contains weak hit to
Pfam PF00085: Thioredoxin; contains 2 predicted
transmembrane domains
Length = 484
Score = 28.7 bits (61), Expect = 3.7
Identities = 17/53 (32%), Positives = 26/53 (49%)
Frame = -1
Query: 191 FARRHGVSNDRGLHAHSSQYEERG*GREILVALVKELQIGRVLDRSSHSQGVD 33
F R G+ D G H H S Y +R + LV +V+EL + + + H +D
Sbjct: 228 FRRGSGLREDHGNHEHESYYGDR--DTDSLVKMVEELL--KPIKKEDHKLALD 276
>At1g65610.1 68414.m07442 endo-1,4-beta-glucanase, putative /
cellulase, putative similar to endo-1,4-beta-glucanase
GI:2065530 from [Lycopersicon esculentum]
Length = 623
Score = 28.3 bits (60), Expect = 4.9
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +3
Query: 402 EVSVDPAIARTLSASETHRSEILMPSWNNAPPKTRL 509
+ + P++ +T A +R E+++PSWNN P L
Sbjct: 354 QFATTPSVPQTAKAF-ANRPELMVPSWNNKLPGAML 388
>At1g63530.1 68414.m07182 hypothetical protein
Length = 499
Score = 27.9 bits (59), Expect = 6.5
Identities = 18/54 (33%), Positives = 29/54 (53%)
Frame = -3
Query: 483 STTASGFRFGAFPTPIAFLLLRGPPKPPWPSTKMCCWVRHTNCLFWNISLFTMA 322
+TT+ G + PTPI+FL P PP PS+ + ++ W+IS ++ A
Sbjct: 80 ATTSVGVSSTSTPTPISFLPFGYVPPPP-PSSSLSGPAQNVP---WSISAYSTA 129
>At5g57740.1 68418.m07218 zinc finger (C3HC4-type RING finger)
family protein / ankyrin repeat family protein contains
Pfam profile: PF00097 zinc finger, C3HC4 type (RING
finger) and Pfam profile: PF00023 ankyrin repeat
Length = 508
Score = 27.5 bits (58), Expect = 8.6
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +1
Query: 142 ECAWRPRSLLTPCR 183
EC WR LTPCR
Sbjct: 305 ECGWRENDCLTPCR 318
>At4g03380.1 68417.m00460 expressed protein
Length = 200
Score = 27.5 bits (58), Expect = 8.6
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +3
Query: 369 EPSSTFSC*AKEVSVDPAIARTLSASETHRSEILMPSWNNAPPKT 503
+P S+FS AKE + A+ ++ HRSE + P+ P +T
Sbjct: 146 KPYSSFSRNAKEDDRNDGEAQGTMTNDVHRSEAIQPTLLPGPNQT 190
>At3g16680.1 68416.m02131 expressed protein ; expression supported
by MPSS
Length = 87
Score = 27.5 bits (58), Expect = 8.6
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = +2
Query: 326 IVNSEIFQNKQLVCRTQQHIFV 391
I + E+FQ K+L+ ++H+FV
Sbjct: 40 IFHIEVFQEKELIVNVKEHVFV 61
>At3g09650.1 68416.m01144 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 778
Score = 27.5 bits (58), Expect = 8.6
Identities = 16/61 (26%), Positives = 24/61 (39%)
Frame = +2
Query: 320 VAIVNSEIFQNKQLVCRTQQHIFVLGQGGFGGPRNSKNAIGVGNAPKRNPDAVVEQRTAE 499
+ V I VC T H V GFG R ++ + +R+ V+ + AE
Sbjct: 290 IVFVLERIIDKGIKVCMTTMHSLVAAYVGFGDLRTAERIVQAMREKRRDLCKVLRECNAE 349
Query: 500 D 502
D
Sbjct: 350 D 350
>At2g45460.1 68415.m05654 forkhead-associated domain-containing
protein / FHA domain-containing protein
Length = 915
Score = 27.5 bits (58), Expect = 8.6
Identities = 16/46 (34%), Positives = 22/46 (47%)
Frame = +1
Query: 529 DLNPLHIDPNVATASGHKKPILHGLASLGFSARHVLAKFGGNDSSN 666
DLNP++ PN A G K+ H L F+AR + G + N
Sbjct: 574 DLNPINRSPNRANTQGDKRATSH----LNFAARASSSTSGQRSTRN 615
>At2g44030.1 68415.m05474 kelch repeat-containing F-box family
protein low similarity to SKP1 interacting partner 6
[Arabidopsis thaliana] GI:10716957; contains Pfam
profiles PF01344: Kelch motif, PF00646: F-box domain
Length = 380
Score = 27.5 bits (58), Expect = 8.6
Identities = 24/89 (26%), Positives = 38/89 (42%), Gaps = 4/89 (4%)
Frame = +3
Query: 60 VENPTDLKFLYESHENFSPLPSFFILAGMCMEAPIVANAMP-PGKFADFTNVLHGEQYIE 236
++N T L + N +P PS+FIL+ I ++P P + + V+ I
Sbjct: 66 LKNETCLYVCLNLNNNNNPNPSWFILSQTPKHKLIPLPSLPYPDPHPNCSTVVSTGSEIY 125
Query: 237 FVGDFPAPRESSQPVVTL---SISWTRAP 314
+G F A + S+ L S W R P
Sbjct: 126 LLGGFVAKEKRSRRAYVLDCKSHQWRRLP 154
>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
similar to GTL1 [Arabidopsis thaliana] GI:2664198
Length = 669
Score = 27.5 bits (58), Expect = 8.6
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +3
Query: 252 PAPRESSQPVVTLSISWTRAPAPWPL 329
P P+ +QP T ++S+T P P PL
Sbjct: 183 PQPQTQTQPPQTHNVSFTPTPPPLPL 208
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,698,222
Number of Sequences: 28952
Number of extensions: 399773
Number of successful extensions: 1306
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1304
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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