Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0148
(810 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATP... 77 2e-14
At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putati... 75 6e-14
At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putati... 65 5e-11
At5g05400.1 68418.m00582 disease resistance protein (CC-NBS-LRR ... 29 2.8
At1g64940.1 68414.m07361 cytochrome P450, putative similar to cy... 29 2.8
At5g26680.1 68418.m03171 endonuclease, putative similar to Swiss... 29 3.7
At4g27180.1 68417.m03904 kinesin-like protein B (KATB) 29 3.7
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 29 4.8
At3g03790.2 68416.m00389 ankyrin repeat family protein / regulat... 29 4.8
At3g03790.1 68416.m00388 ankyrin repeat family protein / regulat... 29 4.8
At3g58810.2 68416.m06555 zinc transporter, putative similar to z... 28 6.4
At3g58810.1 68416.m06554 zinc transporter, putative similar to z... 28 6.4
At5g61560.1 68418.m07725 protein kinase family protein contains ... 28 8.4
At3g56780.1 68416.m06315 F-box family protein contains F-box dom... 28 8.4
At1g62730.1 68414.m07080 expressed protein 28 8.4
>At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATPase
E subunit / vacuolar proton pump E subunit (VATE)
identical to SP|Q39258 Vacuolar ATP synthase subunit E
(EC 3.6.3.14) (V-ATPase E subunit) (Vacuolar proton pump
E subunit) {Arabidopsis thaliana}
Length = 230
Score = 76.6 bits (180), Expect = 2e-14
Identities = 42/95 (44%), Positives = 60/95 (63%)
Frame = +1
Query: 226 QDYGIL*KEGKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKL 405
QDY K+ KQ +++KKI S LN +R+KVL+ ++D V + D+A K L V +D
Sbjct: 53 QDYE---KKEKQADVRKKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYA 109
Query: 406 YSELLVTLIVQALFQLMEPTVTIRVRQTDKALVES 510
Y +LL LIVQ L +L EP+V +R R+ D LVE+
Sbjct: 110 YKQLLKDLIVQCLLRLKEPSVLLRCREEDLGLVEA 144
Score = 43.6 bits (98), Expect = 2e-04
Identities = 22/58 (37%), Positives = 32/58 (55%)
Frame = +2
Query: 80 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYYEKK 253
++D DV +QI+ M+ FI Q FNIEK +LV+ ++ KI + YEKK
Sbjct: 1 MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKK 58
Score = 42.3 bits (95), Expect = 4e-04
Identities = 29/83 (34%), Positives = 45/83 (54%), Gaps = 11/83 (13%)
Frame = +3
Query: 510 LLGKAQQDYKNKIKKDVV-LKVDTENFLSPDT---------C-GGIELVAARGRIKISNT 656
+L A+++Y K K + VDT+ FL P C GG+ L + G+I NT
Sbjct: 145 VLDDAKEEYAGKAKVHAPEVAVDTKIFLPPPPKSNDPHGLHCSGGVVLASRDGKIVCENT 204
Query: 657 LESRLELIAQQLLPEIRNALFGR 725
L++RL++ + LP IR +LFG+
Sbjct: 205 LDARLDVAFRMKLPVIRKSLFGQ 227
>At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 235
Score = 74.9 bits (176), Expect = 6e-14
Identities = 40/98 (40%), Positives = 62/98 (63%)
Frame = +1
Query: 226 QDYGIL*KEGKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKL 405
QDY ++ KQV+++K+I S LN +R+K L+ ++D V + D A K L V D
Sbjct: 53 QDYD---RKLKQVDIRKRIDYSTQLNASRIKYLQAQDDVVTAMKDSAAKDLLRVSNDKNN 109
Query: 406 YSELLVTLIVQALFQLMEPTVTIRVRQTDKALVESCSE 519
Y +LL +LI+++L +L EP+V +R R+ DK +VES E
Sbjct: 110 YKKLLKSLIIESLLRLKEPSVLLRCREMDKKVVESVIE 147
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/58 (32%), Positives = 32/58 (55%)
Frame = +2
Query: 80 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYYEKK 253
++DADV KQI+ M+ FI Q FNIE+ +L++ + K+ + Y++K
Sbjct: 1 MNDADVSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKLRQDYDRK 58
Score = 39.9 bits (89), Expect = 0.002
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = +3
Query: 594 PDTCGGIELVAARGRIKISNTLESRLELIAQQLLPEIRNALFG 722
P GG+ L + G+I NTL++RL++ +Q LP+IR L G
Sbjct: 186 PHCSGGVVLASQDGKIVCENTLDARLDVAFRQKLPQIRTRLVG 228
>At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putative
/ V-ATPase E subunit, putative / vacuolar proton pump E
subunit, putative similar to SP|Q39258 Vacuolar ATP
synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
(Vacuolar proton pump E subunit) {Arabidopsis thaliana};
contains Pfam profile PF01991: ATP synthase (E/31 kDa)
subunit
Length = 237
Score = 65.3 bits (152), Expect = 5e-11
Identities = 39/94 (41%), Positives = 57/94 (60%), Gaps = 6/94 (6%)
Frame = +1
Query: 247 KEGKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPK------DTKLY 408
K+ KQV+++KKI S LN +R+KVL+ ++D V + +EA K+L +V + Y
Sbjct: 57 KKEKQVDVRKKIDYSMQLNASRIKVLQAQDDIVNAMKEEAAKQLLKVSQHGFFNHHHHQY 116
Query: 409 SELLVTLIVQALFQLMEPTVTIRVRQTDKALVES 510
LL LIVQ L +L EP V +R R+ D +VES
Sbjct: 117 KHLLKDLIVQCLLRLKEPAVLLRCREEDLDIVES 150
Score = 44.0 bits (99), Expect = 1e-04
Identities = 30/83 (36%), Positives = 45/83 (54%), Gaps = 11/83 (13%)
Frame = +3
Query: 510 LLGKAQQDYKNKIKKDVV-LKVDTENFLSPD---------TC-GGIELVAARGRIKISNT 656
+L A ++Y K K + VD + FL P +C GG+ L + G+I NT
Sbjct: 151 MLDDASEEYCKKAKVHAPEIIVDKDIFLPPAPSDDDPHALSCAGGVVLASRDGKIVCENT 210
Query: 657 LESRLELIAQQLLPEIRNALFGR 725
L++RLE+ + LPEIR +LFG+
Sbjct: 211 LDARLEVAFRNKLPEIRKSLFGK 233
Score = 41.9 bits (94), Expect = 5e-04
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = +2
Query: 80 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMEYYEKK 253
++DAD QI+ M+ FI Q FNIEK +LV+ ++ KI + YEKK
Sbjct: 1 MNDADASIQIQQMVRFIRQEAEEKANEISISSEEEFNIEKLQLVEAEKKKIRQEYEKK 58
>At5g05400.1 68418.m00582 disease resistance protein (CC-NBS-LRR
class), putative domain signature CC-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 874
Score = 29.5 bits (63), Expect = 2.8
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +1
Query: 268 LQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEV 387
L+K ++ LK +KV+ED N+LDE ++ L+EV
Sbjct: 39 LKKTVRQLEARRDDLLKRIKVQEDRGLNLLDEVQQWLSEV 78
>At1g64940.1 68414.m07361 cytochrome P450, putative similar to
cytochrome p450 GI:438242 from [Solanum melongena]
Length = 511
Score = 29.5 bits (63), Expect = 2.8
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = +1
Query: 346 RNVLDEARKRLAEVPKDTKLYSELLVTLIVQALFQLMEPTVTIRVRQTDKALVESCSEKL 525
R ++DE +KR +E KD K Y V V L + P ++ + + +V CSE L
Sbjct: 258 RKIVDERKKRSSEEEKDNKEY----VQSYVDTLLDVELPDEKRKLNEDE--IVSLCSEFL 311
Query: 526 NKTT 537
N T
Sbjct: 312 NAGT 315
>At5g26680.1 68418.m03171 endonuclease, putative similar to
Swiss-Prot:P39748 FLAP endonuclease-1 (Maturation factor
1) (MF1) [Homo sapiens]
Length = 453
Score = 29.1 bits (62), Expect = 3.7
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = -2
Query: 680 DQLQARLQSVADLDTSPGCNQLDTTTGVGRQKVLSVDFQH 561
++LQ + DL GC+ D+ G+G Q L + QH
Sbjct: 215 EELQLTMDQFIDLCILSGCDYCDSIRGIGGQTALKLIRQH 254
>At4g27180.1 68417.m03904 kinesin-like protein B (KATB)
Length = 745
Score = 29.1 bits (62), Expect = 3.7
Identities = 17/43 (39%), Positives = 29/43 (67%), Gaps = 4/43 (9%)
Frame = +1
Query: 271 QKKIQSSNMLNQ---ARLKV-LKVREDHVRNVLDEARKRLAEV 387
Q+K++++ +N+ A L+V LKV+E+ + V+DE RK A V
Sbjct: 87 QEKLKNAMEMNEKHCADLEVNLKVKEEELNMVIDELRKNFASV 129
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 28.7 bits (61), Expect = 4.8
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = +3
Query: 492 QGSGGVLLGKAQQDYKNKIKKDVVLKVDTENFLSPDTCGGIELVAARGRIKISNTLESR 668
QG+ G + + N + +D+ L++ TE L GG E V GRI + + +R
Sbjct: 280 QGAAGGAEARPFVTFHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRIFRNEGISTR 338
>At3g03790.2 68416.m00389 ankyrin repeat family protein / regulator
of chromosome condensation (RCC1) family protein similar
to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
contains Pfam PF00415: Regulator of chromosome
condensation (RCC1); contains Pfam PF00023: Ankyrin
repeat; similar to rjs (GI:3414809) [Mus musculus];
similar to HERC2 (GI:4079809) [Homo sapiens]
Length = 1081
Score = 28.7 bits (61), Expect = 4.8
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 22 ALYRPFFVLVVLKISSSHGAQRCRCSETDQAHDG 123
+LY P + +VLK S + A +CR E ++ +G
Sbjct: 508 SLYHPAYAPIVLKKSQTLQADKCREEENEELDEG 541
>At3g03790.1 68416.m00388 ankyrin repeat family protein / regulator
of chromosome condensation (RCC1) family protein similar
to hect domain and RLD 2 GB:NP_004658 [Homo sapiens];
contains Pfam PF00415: Regulator of chromosome
condensation (RCC1); contains Pfam PF00023: Ankyrin
repeat; similar to rjs (GI:3414809) [Mus musculus];
similar to HERC2 (GI:4079809) [Homo sapiens]
Length = 1078
Score = 28.7 bits (61), Expect = 4.8
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +1
Query: 22 ALYRPFFVLVVLKISSSHGAQRCRCSETDQAHDG 123
+LY P + +VLK S + A +CR E ++ +G
Sbjct: 505 SLYHPAYAPIVLKKSQTLQADKCREEENEELDEG 538
>At3g58810.2 68416.m06555 zinc transporter, putative similar to zinc
transporter 4; ZnT4 [Mus musculus]
gi|2582990|gb|AAB82593; similar to zinc transporter ZAT
[Arabidopsis thaliana] gi|4206640|gb|AAD11757; member of
the cation diffusion facilitator (CDF) family, or cation
efflux (CE) family, PMID:11500563
Length = 393
Score = 28.3 bits (60), Expect = 6.4
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3
Query: 394 PLALQPGACELHQVHYVRDLHALSVPSDELGSAC 293
P L+ G CE+ +V V +LH ++ +L AC
Sbjct: 323 PTMLEKGVCEIEEVVAVHELHIWAITVGKLLLAC 356
>At3g58810.1 68416.m06554 zinc transporter, putative similar to zinc
transporter 4; ZnT4 [Mus musculus]
gi|2582990|gb|AAB82593; similar to zinc transporter ZAT
[Arabidopsis thaliana] gi|4206640|gb|AAD11757; member of
the cation diffusion facilitator (CDF) family, or cation
efflux (CE) family, PMID:11500563
Length = 432
Score = 28.3 bits (60), Expect = 6.4
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = -3
Query: 394 PLALQPGACELHQVHYVRDLHALSVPSDELGSAC 293
P L+ G CE+ +V V +LH ++ +L AC
Sbjct: 362 PTMLEKGVCEIEEVVAVHELHIWAITVGKLLLAC 395
>At5g61560.1 68418.m07725 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 796
Score = 27.9 bits (59), Expect = 8.4
Identities = 15/60 (25%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
Frame = +1
Query: 250 EGKQVELQKKIQSSNMLNQ---ARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSELL 420
+ + ++ KK+Q N RLK L +RE+ V++ R+R + + +L E +
Sbjct: 317 QSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEADEVVEMERERQEDAENEAELVRECI 376
>At3g56780.1 68416.m06315 F-box family protein contains F-box domain
Pfam:PF00646
Length = 431
Score = 27.9 bits (59), Expect = 8.4
Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 3/46 (6%)
Frame = -1
Query: 633 PWLQPARYHHRC--RATKSSQCRLSTQRPS*SYSC-SLVELFRAGL 505
PW++ A +HH C R + C + T P ++C +LV L G+
Sbjct: 93 PWIRTALHHHHCHLRELEIDACIVHTVLPPELFTCKTLVVLKLKGI 138
>At1g62730.1 68414.m07080 expressed protein
Length = 304
Score = 27.9 bits (59), Expect = 8.4
Identities = 21/89 (23%), Positives = 43/89 (48%), Gaps = 3/89 (3%)
Frame = +1
Query: 211 PAATSQDYGIL*KEGKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVP 390
PA ++ +G+L K+G ++E+ S L+ ++ V H+ + A K AE
Sbjct: 192 PADLAEKHGLLVKQGGRLEILLDNDSREGLSNVVFEIASVANAHLLKARELAGKVPAEA- 250
Query: 391 KDTKLYS---ELLVTLIVQALFQLMEPTV 468
K L+S ++L+ + + F + +P +
Sbjct: 251 KPVLLHSVPVQVLLDSLNKVQFDVFDPRI 279
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,851,559
Number of Sequences: 28952
Number of extensions: 310110
Number of successful extensions: 968
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 942
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 967
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1843581600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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