BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0146
(639 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40810.2 68415.m05035 WD-40 repeat protein family similar to ... 73 2e-13
At2g40810.1 68415.m05034 WD-40 repeat protein family similar to ... 73 2e-13
At3g56440.1 68416.m06277 WD-40 repeat protein family contains 4 ... 72 3e-13
At3g62770.2 68416.m07051 transport protein-related weak similari... 63 1e-10
At5g05150.1 68418.m00547 transport protein-related contains 2 WD... 62 4e-10
At4g30510.2 68417.m04332 transport protein-related weak similari... 59 3e-09
At4g30510.1 68417.m04331 transport protein-related weak similari... 59 3e-09
At3g62770.1 68416.m07052 transport protein-related weak similari... 49 3e-06
At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5 ... 36 0.030
At4g02730.1 68417.m00372 transducin family protein / WD-40 repea... 32 0.28
At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) ne... 32 0.37
At3g49660.1 68416.m05427 transducin family protein / WD-40 repea... 31 0.49
At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 ... 31 0.49
At1g71692.1 68414.m08279 MADS-box protein (AGL12) identical to G... 31 0.49
At5g54730.1 68418.m06815 expressed protein 29 3.4
At4g00090.1 68417.m00009 transducin family protein / WD-40 repea... 28 4.5
At5g24710.1 68418.m02919 WD-40 repeat family protein contains 3 ... 28 6.0
At3g18060.1 68416.m02297 transducin family protein / WD-40 repea... 28 6.0
At2g46290.1 68415.m05758 eukaryotic translation initiation facto... 28 6.0
At4g25510.1 68417.m03677 hypothetical protein 27 7.9
>At2g40810.2 68415.m05035 WD-40 repeat protein family similar to
Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam
PF00400: WD domain, G-beta repeats
Length = 393
Score = 72.5 bits (170), Expect = 2e-13
Identities = 33/64 (51%), Positives = 45/64 (70%)
Frame = +2
Query: 74 LIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFN 253
+I AH++ + C+ L + G LATASTKGTLIR+F+T G +L E+RRG +A IY I +
Sbjct: 196 IINAHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALS 255
Query: 254 PQVQ 265
P VQ
Sbjct: 256 PNVQ 259
Score = 48.8 bits (111), Expect(2) = 1e-08
Identities = 23/68 (33%), Positives = 38/68 (55%)
Frame = +1
Query: 361 LPKYFSSNWSFCKFTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMIS 540
LPKYFSS WS+ +F + AFG +++ ++ DGS+Y+ F + N EM +
Sbjct: 325 LPKYFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSF-DPVNGGEMGQLE 382
Query: 541 FWRLLKID 564
+ +K+D
Sbjct: 383 YIHFMKMD 390
Score = 27.9 bits (59), Expect(2) = 1e-08
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +1
Query: 268 LCVTSDHGTVHVFSLEDEKLNKQS 339
L V+SD GTVH+FSL + + S
Sbjct: 261 LAVSSDKGTVHIFSLRVRVVGEDS 284
>At2g40810.1 68415.m05034 WD-40 repeat protein family similar to
Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam
PF00400: WD domain, G-beta repeats
Length = 393
Score = 72.5 bits (170), Expect = 2e-13
Identities = 33/64 (51%), Positives = 45/64 (70%)
Frame = +2
Query: 74 LIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFN 253
+I AH++ + C+ L + G LATASTKGTLIR+F+T G +L E+RRG +A IY I +
Sbjct: 196 IINAHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALS 255
Query: 254 PQVQ 265
P VQ
Sbjct: 256 PNVQ 259
Score = 48.8 bits (111), Expect(2) = 1e-08
Identities = 23/68 (33%), Positives = 38/68 (55%)
Frame = +1
Query: 361 LPKYFSSNWSFCKFTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMIS 540
LPKYFSS WS+ +F + AFG +++ ++ DGS+Y+ F + N EM +
Sbjct: 325 LPKYFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSF-DPVNGGEMGQLE 382
Query: 541 FWRLLKID 564
+ +K+D
Sbjct: 383 YIHFMKMD 390
Score = 27.9 bits (59), Expect(2) = 1e-08
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +1
Query: 268 LCVTSDHGTVHVFSLEDEKLNKQS 339
L V+SD GTVH+FSL + + S
Sbjct: 261 LAVSSDKGTVHIFSLRVRVVGEDS 284
>At3g56440.1 68416.m06277 WD-40 repeat protein family contains 4
WD-40 repeats (PF00400) (2 weak); PS00778 Histidine acid
phosphatases active site signature; similar to Gsa12p
(GI:18307769) {Pichia pastoris}similar to
uncharacterized protein JM5 (GP:3114828) [Homo sapiens]
Length = 391
Score = 72.1 bits (169), Expect = 3e-13
Identities = 33/64 (51%), Positives = 45/64 (70%)
Frame = +2
Query: 74 LIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFN 253
+I AH++ + C+ L + G LATASTKGTLIR+F+T G +L E+RRG +A IY I +
Sbjct: 200 IINAHDSNIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALS 259
Query: 254 PQVQ 265
P VQ
Sbjct: 260 PNVQ 263
Score = 53.2 bits (122), Expect = 1e-07
Identities = 27/68 (39%), Positives = 39/68 (57%)
Frame = +1
Query: 361 LPKYFSSNWSFCKFTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMIS 540
LPKYFSS WSF +F +P AFG +++I ++ DGS+Y+ F + N EM +
Sbjct: 323 LPKYFSSEWSFSQFHVPEVTQYFAAFGA-QNTIAIIGLDGSFYRCNF-DPVNGGEMTQLE 380
Query: 541 FWRLLKID 564
+ LK D
Sbjct: 381 HFHFLKQD 388
>At3g62770.2 68416.m07051 transport protein-related weak similarity
to Gsa12p [Pichia pastoris] GI:18307769; contains 1
WD-40 repeat (PF00400); putative proteins - different
species
Length = 432
Score = 63.3 bits (147), Expect = 1e-10
Identities = 39/118 (33%), Positives = 61/118 (51%), Gaps = 16/118 (13%)
Frame = +1
Query: 268 LCVTSDHGTVHVFSL---------EDEKL-------NKQSTLATVNFLPKYFSSNWSFCK 399
L V+SD GTVHVF L + ++ + S+L+ LP+YFSS WS +
Sbjct: 316 LAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKVLPRYFSSEWSVAQ 375
Query: 400 FTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMISFWRLLKIDKYF 573
F + G I AFG K++++++ DGS+Y+ +F + N EM + + LK F
Sbjct: 376 FRLVEGTQYIAAFGHQKNTVVILGMDGSFYRCQF-DPVNGGEMSQLEYHNCLKPPSVF 432
Score = 48.0 bits (109), Expect = 5e-06
Identities = 27/71 (38%), Positives = 39/71 (54%), Gaps = 8/71 (11%)
Frame = +2
Query: 77 IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAEL--------RRGAHQAT 232
+ AH++ + C AL G LATAS+KGTL+R+F+T G E + GA +A
Sbjct: 244 VMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQESGTSEDEIGKEGADRAE 303
Query: 233 IYCINFNPQVQ 265
IY + F+ Q
Sbjct: 304 IYSLAFSSNAQ 314
>At5g05150.1 68418.m00547 transport protein-related contains 2 WD-40
repeats (PF00400); similar to transport protein Gsa12p
(GI:18307769) [Pichia pastoris]
Length = 374
Score = 61.7 bits (143), Expect = 4e-10
Identities = 28/54 (51%), Positives = 37/54 (68%)
Frame = +2
Query: 77 IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIY 238
I AH++ + C+ L + G+ LATASTKGTLIR+F+ G L E RRG +A IY
Sbjct: 200 IKAHDSAIACMTLTLDGSLLATASTKGTLIRIFNAVDGTLLQEFRRGVERAEIY 253
Score = 43.6 bits (98), Expect = 1e-04
Identities = 27/84 (32%), Positives = 48/84 (57%), Gaps = 5/84 (5%)
Frame = +1
Query: 268 LCVTSDHGTVHVFSLEDEKLN----KQSTLATVNFLPKY-FSSNWSFCKFTIPNGPPCIC 432
+ +S+ GT+HVF L + L+ S+ V LPKY + + SF +F++P I
Sbjct: 264 VAASSEKGTLHVFRLRPDILSFDPASSSSFIRV-ILPKYLYENERSFAQFSLPASTKFIV 322
Query: 433 AFGVDKSSIIVVCADGSYYKYKFN 504
FG ++++++V DGS+ + KF+
Sbjct: 323 GFG-SENTVLLVGIDGSFRRCKFD 345
>At4g30510.2 68417.m04332 transport protein-related weak similarity
to Gsa12p [Pichia pastoris] GI:18307769; contains 1
WD-40 repeat (PF00400); similar to several hypothetical
proteins
Length = 261
Score = 58.8 bits (136), Expect = 3e-09
Identities = 30/69 (43%), Positives = 41/69 (59%), Gaps = 1/69 (1%)
Frame = +2
Query: 65 ESHL-IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYC 241
+SH I AH +PL +AL+ G +AT S +GTLIRV S K RRG + +TIY
Sbjct: 130 QSHSEIDAHRSPLAAIALSSNGMYIATGSEQGTLIRVHLVSEATKSYSFRRGTYPSTIYS 189
Query: 242 INFNPQVQI 268
++F P Q+
Sbjct: 190 LSFGPSTQL 198
>At4g30510.1 68417.m04331 transport protein-related weak similarity
to Gsa12p [Pichia pastoris] GI:18307769; contains 1
WD-40 repeat (PF00400); similar to several hypothetical
proteins
Length = 312
Score = 58.8 bits (136), Expect = 3e-09
Identities = 30/69 (43%), Positives = 41/69 (59%), Gaps = 1/69 (1%)
Frame = +2
Query: 65 ESHL-IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYC 241
+SH I AH +PL +AL+ G +AT S +GTLIRV S K RRG + +TIY
Sbjct: 117 QSHSEIDAHRSPLAAIALSSNGMYIATGSEQGTLIRVHLVSEATKSYSFRRGTYPSTIYS 176
Query: 242 INFNPQVQI 268
++F P Q+
Sbjct: 177 LSFGPSTQL 185
>At3g62770.1 68416.m07052 transport protein-related weak similarity
to Gsa12p [Pichia pastoris] GI:18307769; contains 1
WD-40 repeat (PF00400); putative proteins - different
species
Length = 307
Score = 48.8 bits (111), Expect = 3e-06
Identities = 22/46 (47%), Positives = 31/46 (67%)
Frame = +2
Query: 77 IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRR 214
+ AH++ + C AL G LATAS+KGTL+R+F+T G E+RR
Sbjct: 244 VMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRR 289
>At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5
WD-40 repeats (PF0400); similar to WD-40 repeat protein
MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 496
Score = 35.5 bits (78), Expect = 0.030
Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 4/75 (5%)
Frame = +2
Query: 44 AGPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFD----TSTGQKLAELR 211
A T+P + AH+A L C+ N L + +RVFD TS G +
Sbjct: 311 ARTGTSPAMKVEKAHDADLHCVDWNPHDNNLILTGSADNTVRVFDRRNLTSNGVGSPVYK 370
Query: 212 RGAHQATIYCINFNP 256
H+A + C+ ++P
Sbjct: 371 FEGHRAAVLCVQWSP 385
Score = 27.5 bits (58), Expect = 7.9
Identities = 16/70 (22%), Positives = 31/70 (44%)
Frame = +2
Query: 47 GPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQ 226
GPS P + H+ + +A + + + + ++D TG A AH
Sbjct: 268 GPSVGPRG-IYNGHKDTVEDVAFCPSSAQEFCSVGDDSCLMLWDARTGTSPAMKVEKAHD 326
Query: 227 ATIYCINFNP 256
A ++C+++NP
Sbjct: 327 ADLHCVDWNP 336
>At4g02730.1 68417.m00372 transducin family protein / WD-40 repeat
family protein similar to C. elegans putative WD-repeat
protein C14B1.4 (SP:Q17963)
Length = 333
Score = 32.3 bits (70), Expect = 0.28
Identities = 19/68 (27%), Positives = 33/68 (48%)
Frame = +2
Query: 71 HLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINF 250
H H + + LA + +AS TL R++D + + ++ RG H ++C+NF
Sbjct: 79 HRYEGHSSGISDLAWSSDSHYTCSASDDCTL-RIWDARSPYECLKVLRG-HTNFVFCVNF 136
Query: 251 NPQVQIFV 274
NP + V
Sbjct: 137 NPPSNLIV 144
>At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K)
nearly identical to gi:4467359
Length = 1121
Score = 31.9 bits (69), Expect = 0.37
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +1
Query: 268 LCVTSDHGTVHVFSLEDEKLNKQSTLATVNF 360
LC TS +G V +F +ED K+ + +LA +NF
Sbjct: 561 LCETS-YGLVDIFPIEDRKIGLRESLAEINF 590
>At3g49660.1 68416.m05427 transducin family protein / WD-40 repeat
family protein beta-transducin, Schizosaccharomyces
pombe, EMBL:CAA17803
Length = 317
Score = 31.5 bits (68), Expect = 0.49
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +2
Query: 86 HEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFNPQVQ 265
HE + +A + + +AS TL +++D TG + L H +C+NFNPQ
Sbjct: 70 HENGISDVAFSSDARFIVSASDDKTL-KLWDVETGSLIKTLI--GHTNYAFCVNFNPQSN 126
Query: 266 IFV 274
+ V
Sbjct: 127 MIV 129
>At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 (4
significant) WD-40 repeats (PF0400); identical to WD-40
repeat protein MSI4 (SP:O22607) [Arabidopsis thaliana]
Length = 507
Score = 31.5 bits (68), Expect = 0.49
Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 4/75 (5%)
Frame = +2
Query: 44 AGPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFD----TSTGQKLAELR 211
A T P + + AH+A L C+ N L + +R+FD T+ G +
Sbjct: 322 ARTGTNPVTKVEKAHDADLHCVDWNPHDDNLILTGSADNTVRLFDRRKLTANGVGSPIYK 381
Query: 212 RGAHQATIYCINFNP 256
H+A + C+ ++P
Sbjct: 382 FEGHKAAVLCVQWSP 396
>At1g71692.1 68414.m08279 MADS-box protein (AGL12) identical to
GB:AAC49085 GI:862650 from (Arabidopsis thaliana) (Plant
Cell 7 (8), 1259-1269 (1995))
Length = 211
Score = 31.5 bits (68), Expect = 0.49
Identities = 21/55 (38%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
Frame = -2
Query: 458 IELLSTPNAQMQGGPFGIVNLQKLQLLEKYLG---KKLTVAKVDCLLSFSSSKLN 303
IE+L + M GG G +NL++L LLEK+L ++ AK+D +L S N
Sbjct: 109 IEMLQKGISYMFGGGDGAMNLEELLLLEKHLEYWISQIRSAKMDVMLQEIQSLRN 163
>At5g54730.1 68418.m06815 expressed protein
Length = 763
Score = 28.7 bits (61), Expect = 3.4
Identities = 24/76 (31%), Positives = 33/76 (43%), Gaps = 12/76 (15%)
Frame = +2
Query: 83 AHEAPLCCLALNVGGTRLATASTKGTLIRVF------------DTSTGQKLAELRRGAHQ 226
AH++P+ L + G L TAS +G I VF T+T L L+RG
Sbjct: 345 AHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTSRAVKTTTFAHLFRLQRGFTN 404
Query: 227 ATIYCINFNPQVQIFV 274
A I I F+ + V
Sbjct: 405 AVIQDICFSKDSNLIV 420
>At4g00090.1 68417.m00009 transducin family protein / WD-40 repeat
family protein similar to Transducin beta-like 2 protein
(WS beta-transducin repeats protein) (WS-betaTRP)
(Williams-Beuren syndrome chromosome region 13 protein)
(SP:Q9Y4P3) {Homo sapiens}
Length = 430
Score = 28.3 bits (60), Expect = 4.5
Identities = 16/44 (36%), Positives = 22/44 (50%), Gaps = 4/44 (9%)
Frame = +1
Query: 376 SSNWSFCKFTIP-NGPPCICAFGVDKSSIIVVC---ADGSYYKY 495
S ++ F + +P G P AF D SSI+V C + S Y Y
Sbjct: 120 SKSFKFLRINLPAGGHPTAVAFADDASSIVVACHHMSGSSLYMY 163
>At5g24710.1 68418.m02919 WD-40 repeat family protein contains 3
Pfam PF00400: WD domain, G-beta repeats;
Length = 1327
Score = 27.9 bits (59), Expect = 6.0
Identities = 19/43 (44%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = +2
Query: 125 GTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCI-NF 250
G +A ST G +IRV T KLA G H+ +IYC+ NF
Sbjct: 101 GPLVAFGSTDG-VIRVLSMITW-KLARRYTGGHKGSIYCLMNF 141
>At3g18060.1 68416.m02297 transducin family protein / WD-40 repeat
family protein similar to 66 kDa stress protein
(SP:P90587) [Physarum polycephalum (Slime mold)];
similar to WDR1 protein GB:AAD05042 [Gallus gallus]
(Genomics 56 (1), 59-69 (1999)); contains 11 WD-40
repeats (PF00400)
Length = 609
Score = 27.9 bits (59), Expect = 6.0
Identities = 16/55 (29%), Positives = 25/55 (45%)
Frame = +2
Query: 86 HEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINF 250
H A + CLA + T +AT S T + V++ +GAH +Y + F
Sbjct: 533 HSARINCLAWSPNSTMVATGSL-DTCVIVYEVDKPASSRMTIKGAHLGGVYGLGF 586
>At2g46290.1 68415.m05758 eukaryotic translation initiation factor 3
subunit 2, putative / eIF-3 beta, putative / eIF3i,
putative strong similarity to SP|Q38884 Eukaryotic
translation initiation factor 3 subunit 2 (eIF-3 beta)
(eIF3 p36) (eIF3i) (TGF-beta receptor interacting
protein 1) (TRIP-1) {Arabidopsis thaliana}; contains
Pfam PF00400: WD domain, G-beta repeat (5
copies)|19799885|gb|AU231175.1|AU231175
Length = 355
Score = 27.9 bits (59), Expect = 6.0
Identities = 20/73 (27%), Positives = 30/73 (41%)
Frame = +2
Query: 35 SSHAGPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRR 214
SSH T L+ HE PL L N G L + + K V+ G++L R
Sbjct: 19 SSHQTLETRMRPILMKGHERPLTFLRYNRNGDLLFSCA-KDHTPTVWFADNGERLGTYR- 76
Query: 215 GAHQATIYCINFN 253
H ++C + +
Sbjct: 77 -GHSGAVWCCDIS 88
>At4g25510.1 68417.m03677 hypothetical protein
Length = 149
Score = 27.5 bits (58), Expect = 7.9
Identities = 13/45 (28%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Frame = +3
Query: 183 QQDRNWLNCEEELIRQLYTALILTHKYKSLCD--LRSWNCSCIQF 311
Q+ NW N ++ I +LY++++L +KY D +++ C Q+
Sbjct: 80 QRSENWRNPVDKEIIKLYSSILLGNKYTKADDQCRQNYKSICSQY 124
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,280,462
Number of Sequences: 28952
Number of extensions: 265772
Number of successful extensions: 702
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 668
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 701
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1314848736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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