BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0146 (639 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g40810.2 68415.m05035 WD-40 repeat protein family similar to ... 73 2e-13 At2g40810.1 68415.m05034 WD-40 repeat protein family similar to ... 73 2e-13 At3g56440.1 68416.m06277 WD-40 repeat protein family contains 4 ... 72 3e-13 At3g62770.2 68416.m07051 transport protein-related weak similari... 63 1e-10 At5g05150.1 68418.m00547 transport protein-related contains 2 WD... 62 4e-10 At4g30510.2 68417.m04332 transport protein-related weak similari... 59 3e-09 At4g30510.1 68417.m04331 transport protein-related weak similari... 59 3e-09 At3g62770.1 68416.m07052 transport protein-related weak similari... 49 3e-06 At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5 ... 36 0.030 At4g02730.1 68417.m00372 transducin family protein / WD-40 repea... 32 0.28 At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) ne... 32 0.37 At3g49660.1 68416.m05427 transducin family protein / WD-40 repea... 31 0.49 At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 ... 31 0.49 At1g71692.1 68414.m08279 MADS-box protein (AGL12) identical to G... 31 0.49 At5g54730.1 68418.m06815 expressed protein 29 3.4 At4g00090.1 68417.m00009 transducin family protein / WD-40 repea... 28 4.5 At5g24710.1 68418.m02919 WD-40 repeat family protein contains 3 ... 28 6.0 At3g18060.1 68416.m02297 transducin family protein / WD-40 repea... 28 6.0 At2g46290.1 68415.m05758 eukaryotic translation initiation facto... 28 6.0 At4g25510.1 68417.m03677 hypothetical protein 27 7.9 >At2g40810.2 68415.m05035 WD-40 repeat protein family similar to Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam PF00400: WD domain, G-beta repeats Length = 393 Score = 72.5 bits (170), Expect = 2e-13 Identities = 33/64 (51%), Positives = 45/64 (70%) Frame = +2 Query: 74 LIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFN 253 +I AH++ + C+ L + G LATASTKGTLIR+F+T G +L E+RRG +A IY I + Sbjct: 196 IINAHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALS 255 Query: 254 PQVQ 265 P VQ Sbjct: 256 PNVQ 259 Score = 48.8 bits (111), Expect(2) = 1e-08 Identities = 23/68 (33%), Positives = 38/68 (55%) Frame = +1 Query: 361 LPKYFSSNWSFCKFTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMIS 540 LPKYFSS WS+ +F + AFG +++ ++ DGS+Y+ F + N EM + Sbjct: 325 LPKYFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSF-DPVNGGEMGQLE 382 Query: 541 FWRLLKID 564 + +K+D Sbjct: 383 YIHFMKMD 390 Score = 27.9 bits (59), Expect(2) = 1e-08 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = +1 Query: 268 LCVTSDHGTVHVFSLEDEKLNKQS 339 L V+SD GTVH+FSL + + S Sbjct: 261 LAVSSDKGTVHIFSLRVRVVGEDS 284 >At2g40810.1 68415.m05034 WD-40 repeat protein family similar to Gsa12p(GI:18307769)[Pichia pastoris]; contains 3 Pfam PF00400: WD domain, G-beta repeats Length = 393 Score = 72.5 bits (170), Expect = 2e-13 Identities = 33/64 (51%), Positives = 45/64 (70%) Frame = +2 Query: 74 LIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFN 253 +I AH++ + C+ L + G LATASTKGTLIR+F+T G +L E+RRG +A IY I + Sbjct: 196 IINAHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALS 255 Query: 254 PQVQ 265 P VQ Sbjct: 256 PNVQ 259 Score = 48.8 bits (111), Expect(2) = 1e-08 Identities = 23/68 (33%), Positives = 38/68 (55%) Frame = +1 Query: 361 LPKYFSSNWSFCKFTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMIS 540 LPKYFSS WS+ +F + AFG +++ ++ DGS+Y+ F + N EM + Sbjct: 325 LPKYFSSEWSYAQFHVSEVTQFFAAFG-SNNTVAIIGMDGSFYRCSF-DPVNGGEMGQLE 382 Query: 541 FWRLLKID 564 + +K+D Sbjct: 383 YIHFMKMD 390 Score = 27.9 bits (59), Expect(2) = 1e-08 Identities = 12/24 (50%), Positives = 16/24 (66%) Frame = +1 Query: 268 LCVTSDHGTVHVFSLEDEKLNKQS 339 L V+SD GTVH+FSL + + S Sbjct: 261 LAVSSDKGTVHIFSLRVRVVGEDS 284 >At3g56440.1 68416.m06277 WD-40 repeat protein family contains 4 WD-40 repeats (PF00400) (2 weak); PS00778 Histidine acid phosphatases active site signature; similar to Gsa12p (GI:18307769) {Pichia pastoris}similar to uncharacterized protein JM5 (GP:3114828) [Homo sapiens] Length = 391 Score = 72.1 bits (169), Expect = 3e-13 Identities = 33/64 (51%), Positives = 45/64 (70%) Frame = +2 Query: 74 LIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFN 253 +I AH++ + C+ L + G LATASTKGTLIR+F+T G +L E+RRG +A IY I + Sbjct: 200 IINAHDSNIACMTLTLDGLLLATASTKGTLIRIFNTMDGTRLQEVRRGVDRADIYSIALS 259 Query: 254 PQVQ 265 P VQ Sbjct: 260 PNVQ 263 Score = 53.2 bits (122), Expect = 1e-07 Identities = 27/68 (39%), Positives = 39/68 (57%) Frame = +1 Query: 361 LPKYFSSNWSFCKFTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMIS 540 LPKYFSS WSF +F +P AFG +++I ++ DGS+Y+ F + N EM + Sbjct: 323 LPKYFSSEWSFSQFHVPEVTQYFAAFGA-QNTIAIIGLDGSFYRCNF-DPVNGGEMTQLE 380 Query: 541 FWRLLKID 564 + LK D Sbjct: 381 HFHFLKQD 388 >At3g62770.2 68416.m07051 transport protein-related weak similarity to Gsa12p [Pichia pastoris] GI:18307769; contains 1 WD-40 repeat (PF00400); putative proteins - different species Length = 432 Score = 63.3 bits (147), Expect = 1e-10 Identities = 39/118 (33%), Positives = 61/118 (51%), Gaps = 16/118 (13%) Frame = +1 Query: 268 LCVTSDHGTVHVFSL---------EDEKL-------NKQSTLATVNFLPKYFSSNWSFCK 399 L V+SD GTVHVF L + ++ + S+L+ LP+YFSS WS + Sbjct: 316 LAVSSDKGTVHVFGLKVNSGSQVKDSSRIAPDATPSSPSSSLSLFKVLPRYFSSEWSVAQ 375 Query: 400 FTIPNGPPCICAFGVDKSSIIVVCADGSYYKYKFNERGNAPEMFMISFWRLLKIDKYF 573 F + G I AFG K++++++ DGS+Y+ +F + N EM + + LK F Sbjct: 376 FRLVEGTQYIAAFGHQKNTVVILGMDGSFYRCQF-DPVNGGEMSQLEYHNCLKPPSVF 432 Score = 48.0 bits (109), Expect = 5e-06 Identities = 27/71 (38%), Positives = 39/71 (54%), Gaps = 8/71 (11%) Frame = +2 Query: 77 IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAEL--------RRGAHQAT 232 + AH++ + C AL G LATAS+KGTL+R+F+T G E + GA +A Sbjct: 244 VMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQESGTSEDEIGKEGADRAE 303 Query: 233 IYCINFNPQVQ 265 IY + F+ Q Sbjct: 304 IYSLAFSSNAQ 314 >At5g05150.1 68418.m00547 transport protein-related contains 2 WD-40 repeats (PF00400); similar to transport protein Gsa12p (GI:18307769) [Pichia pastoris] Length = 374 Score = 61.7 bits (143), Expect = 4e-10 Identities = 28/54 (51%), Positives = 37/54 (68%) Frame = +2 Query: 77 IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIY 238 I AH++ + C+ L + G+ LATASTKGTLIR+F+ G L E RRG +A IY Sbjct: 200 IKAHDSAIACMTLTLDGSLLATASTKGTLIRIFNAVDGTLLQEFRRGVERAEIY 253 Score = 43.6 bits (98), Expect = 1e-04 Identities = 27/84 (32%), Positives = 48/84 (57%), Gaps = 5/84 (5%) Frame = +1 Query: 268 LCVTSDHGTVHVFSLEDEKLN----KQSTLATVNFLPKY-FSSNWSFCKFTIPNGPPCIC 432 + +S+ GT+HVF L + L+ S+ V LPKY + + SF +F++P I Sbjct: 264 VAASSEKGTLHVFRLRPDILSFDPASSSSFIRV-ILPKYLYENERSFAQFSLPASTKFIV 322 Query: 433 AFGVDKSSIIVVCADGSYYKYKFN 504 FG ++++++V DGS+ + KF+ Sbjct: 323 GFG-SENTVLLVGIDGSFRRCKFD 345 >At4g30510.2 68417.m04332 transport protein-related weak similarity to Gsa12p [Pichia pastoris] GI:18307769; contains 1 WD-40 repeat (PF00400); similar to several hypothetical proteins Length = 261 Score = 58.8 bits (136), Expect = 3e-09 Identities = 30/69 (43%), Positives = 41/69 (59%), Gaps = 1/69 (1%) Frame = +2 Query: 65 ESHL-IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYC 241 +SH I AH +PL +AL+ G +AT S +GTLIRV S K RRG + +TIY Sbjct: 130 QSHSEIDAHRSPLAAIALSSNGMYIATGSEQGTLIRVHLVSEATKSYSFRRGTYPSTIYS 189 Query: 242 INFNPQVQI 268 ++F P Q+ Sbjct: 190 LSFGPSTQL 198 >At4g30510.1 68417.m04331 transport protein-related weak similarity to Gsa12p [Pichia pastoris] GI:18307769; contains 1 WD-40 repeat (PF00400); similar to several hypothetical proteins Length = 312 Score = 58.8 bits (136), Expect = 3e-09 Identities = 30/69 (43%), Positives = 41/69 (59%), Gaps = 1/69 (1%) Frame = +2 Query: 65 ESHL-IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYC 241 +SH I AH +PL +AL+ G +AT S +GTLIRV S K RRG + +TIY Sbjct: 117 QSHSEIDAHRSPLAAIALSSNGMYIATGSEQGTLIRVHLVSEATKSYSFRRGTYPSTIYS 176 Query: 242 INFNPQVQI 268 ++F P Q+ Sbjct: 177 LSFGPSTQL 185 >At3g62770.1 68416.m07052 transport protein-related weak similarity to Gsa12p [Pichia pastoris] GI:18307769; contains 1 WD-40 repeat (PF00400); putative proteins - different species Length = 307 Score = 48.8 bits (111), Expect = 3e-06 Identities = 22/46 (47%), Positives = 31/46 (67%) Frame = +2 Query: 77 IAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRR 214 + AH++ + C AL G LATAS+KGTL+R+F+T G E+RR Sbjct: 244 VMAHDSRIACFALTQDGHLLATASSKGTLVRIFNTVDGTLRQEVRR 289 >At4g29730.1 68417.m04233 WD-40 repeat family protein contains 5 WD-40 repeats (PF0400); similar to WD-40 repeat protein MSI4 (SP:O22607) [Arabidopsis thaliana] Length = 496 Score = 35.5 bits (78), Expect = 0.030 Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 4/75 (5%) Frame = +2 Query: 44 AGPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFD----TSTGQKLAELR 211 A T+P + AH+A L C+ N L + +RVFD TS G + Sbjct: 311 ARTGTSPAMKVEKAHDADLHCVDWNPHDNNLILTGSADNTVRVFDRRNLTSNGVGSPVYK 370 Query: 212 RGAHQATIYCINFNP 256 H+A + C+ ++P Sbjct: 371 FEGHRAAVLCVQWSP 385 Score = 27.5 bits (58), Expect = 7.9 Identities = 16/70 (22%), Positives = 31/70 (44%) Frame = +2 Query: 47 GPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQ 226 GPS P + H+ + +A + + + + ++D TG A AH Sbjct: 268 GPSVGPRG-IYNGHKDTVEDVAFCPSSAQEFCSVGDDSCLMLWDARTGTSPAMKVEKAHD 326 Query: 227 ATIYCINFNP 256 A ++C+++NP Sbjct: 327 ADLHCVDWNP 336 >At4g02730.1 68417.m00372 transducin family protein / WD-40 repeat family protein similar to C. elegans putative WD-repeat protein C14B1.4 (SP:Q17963) Length = 333 Score = 32.3 bits (70), Expect = 0.28 Identities = 19/68 (27%), Positives = 33/68 (48%) Frame = +2 Query: 71 HLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINF 250 H H + + LA + +AS TL R++D + + ++ RG H ++C+NF Sbjct: 79 HRYEGHSSGISDLAWSSDSHYTCSASDDCTL-RIWDARSPYECLKVLRG-HTNFVFCVNF 136 Query: 251 NPQVQIFV 274 NP + V Sbjct: 137 NPPSNLIV 144 >At5g64070.1 68418.m08046 phosphatidylinositol 4-kinase (PI4K) nearly identical to gi:4467359 Length = 1121 Score = 31.9 bits (69), Expect = 0.37 Identities = 14/31 (45%), Positives = 21/31 (67%) Frame = +1 Query: 268 LCVTSDHGTVHVFSLEDEKLNKQSTLATVNF 360 LC TS +G V +F +ED K+ + +LA +NF Sbjct: 561 LCETS-YGLVDIFPIEDRKIGLRESLAEINF 590 >At3g49660.1 68416.m05427 transducin family protein / WD-40 repeat family protein beta-transducin, Schizosaccharomyces pombe, EMBL:CAA17803 Length = 317 Score = 31.5 bits (68), Expect = 0.49 Identities = 19/63 (30%), Positives = 31/63 (49%) Frame = +2 Query: 86 HEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINFNPQVQ 265 HE + +A + + +AS TL +++D TG + L H +C+NFNPQ Sbjct: 70 HENGISDVAFSSDARFIVSASDDKTL-KLWDVETGSLIKTLI--GHTNYAFCVNFNPQSN 126 Query: 266 IFV 274 + V Sbjct: 127 MIV 129 >At2g19520.1 68415.m02281 WD-40 repeat protein (MSI4) contains 6 (4 significant) WD-40 repeats (PF0400); identical to WD-40 repeat protein MSI4 (SP:O22607) [Arabidopsis thaliana] Length = 507 Score = 31.5 bits (68), Expect = 0.49 Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 4/75 (5%) Frame = +2 Query: 44 AGPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFD----TSTGQKLAELR 211 A T P + + AH+A L C+ N L + +R+FD T+ G + Sbjct: 322 ARTGTNPVTKVEKAHDADLHCVDWNPHDDNLILTGSADNTVRLFDRRKLTANGVGSPIYK 381 Query: 212 RGAHQATIYCINFNP 256 H+A + C+ ++P Sbjct: 382 FEGHKAAVLCVQWSP 396 >At1g71692.1 68414.m08279 MADS-box protein (AGL12) identical to GB:AAC49085 GI:862650 from (Arabidopsis thaliana) (Plant Cell 7 (8), 1259-1269 (1995)) Length = 211 Score = 31.5 bits (68), Expect = 0.49 Identities = 21/55 (38%), Positives = 31/55 (56%), Gaps = 3/55 (5%) Frame = -2 Query: 458 IELLSTPNAQMQGGPFGIVNLQKLQLLEKYLG---KKLTVAKVDCLLSFSSSKLN 303 IE+L + M GG G +NL++L LLEK+L ++ AK+D +L S N Sbjct: 109 IEMLQKGISYMFGGGDGAMNLEELLLLEKHLEYWISQIRSAKMDVMLQEIQSLRN 163 >At5g54730.1 68418.m06815 expressed protein Length = 763 Score = 28.7 bits (61), Expect = 3.4 Identities = 24/76 (31%), Positives = 33/76 (43%), Gaps = 12/76 (15%) Frame = +2 Query: 83 AHEAPLCCLALNVGGTRLATASTKGTLIRVF------------DTSTGQKLAELRRGAHQ 226 AH++P+ L + G L TAS +G I VF T+T L L+RG Sbjct: 345 AHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTSRAVKTTTFAHLFRLQRGFTN 404 Query: 227 ATIYCINFNPQVQIFV 274 A I I F+ + V Sbjct: 405 AVIQDICFSKDSNLIV 420 >At4g00090.1 68417.m00009 transducin family protein / WD-40 repeat family protein similar to Transducin beta-like 2 protein (WS beta-transducin repeats protein) (WS-betaTRP) (Williams-Beuren syndrome chromosome region 13 protein) (SP:Q9Y4P3) {Homo sapiens} Length = 430 Score = 28.3 bits (60), Expect = 4.5 Identities = 16/44 (36%), Positives = 22/44 (50%), Gaps = 4/44 (9%) Frame = +1 Query: 376 SSNWSFCKFTIP-NGPPCICAFGVDKSSIIVVC---ADGSYYKY 495 S ++ F + +P G P AF D SSI+V C + S Y Y Sbjct: 120 SKSFKFLRINLPAGGHPTAVAFADDASSIVVACHHMSGSSLYMY 163 >At5g24710.1 68418.m02919 WD-40 repeat family protein contains 3 Pfam PF00400: WD domain, G-beta repeats; Length = 1327 Score = 27.9 bits (59), Expect = 6.0 Identities = 19/43 (44%), Positives = 24/43 (55%), Gaps = 1/43 (2%) Frame = +2 Query: 125 GTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCI-NF 250 G +A ST G +IRV T KLA G H+ +IYC+ NF Sbjct: 101 GPLVAFGSTDG-VIRVLSMITW-KLARRYTGGHKGSIYCLMNF 141 >At3g18060.1 68416.m02297 transducin family protein / WD-40 repeat family protein similar to 66 kDa stress protein (SP:P90587) [Physarum polycephalum (Slime mold)]; similar to WDR1 protein GB:AAD05042 [Gallus gallus] (Genomics 56 (1), 59-69 (1999)); contains 11 WD-40 repeats (PF00400) Length = 609 Score = 27.9 bits (59), Expect = 6.0 Identities = 16/55 (29%), Positives = 25/55 (45%) Frame = +2 Query: 86 HEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRRGAHQATIYCINF 250 H A + CLA + T +AT S T + V++ +GAH +Y + F Sbjct: 533 HSARINCLAWSPNSTMVATGSL-DTCVIVYEVDKPASSRMTIKGAHLGGVYGLGF 586 >At2g46290.1 68415.m05758 eukaryotic translation initiation factor 3 subunit 2, putative / eIF-3 beta, putative / eIF3i, putative strong similarity to SP|Q38884 Eukaryotic translation initiation factor 3 subunit 2 (eIF-3 beta) (eIF3 p36) (eIF3i) (TGF-beta receptor interacting protein 1) (TRIP-1) {Arabidopsis thaliana}; contains Pfam PF00400: WD domain, G-beta repeat (5 copies)|19799885|gb|AU231175.1|AU231175 Length = 355 Score = 27.9 bits (59), Expect = 6.0 Identities = 20/73 (27%), Positives = 30/73 (41%) Frame = +2 Query: 35 SSHAGPSTAPESHLIAAHEAPLCCLALNVGGTRLATASTKGTLIRVFDTSTGQKLAELRR 214 SSH T L+ HE PL L N G L + + K V+ G++L R Sbjct: 19 SSHQTLETRMRPILMKGHERPLTFLRYNRNGDLLFSCA-KDHTPTVWFADNGERLGTYR- 76 Query: 215 GAHQATIYCINFN 253 H ++C + + Sbjct: 77 -GHSGAVWCCDIS 88 >At4g25510.1 68417.m03677 hypothetical protein Length = 149 Score = 27.5 bits (58), Expect = 7.9 Identities = 13/45 (28%), Positives = 26/45 (57%), Gaps = 2/45 (4%) Frame = +3 Query: 183 QQDRNWLNCEEELIRQLYTALILTHKYKSLCD--LRSWNCSCIQF 311 Q+ NW N ++ I +LY++++L +KY D +++ C Q+ Sbjct: 80 QRSENWRNPVDKEIIKLYSSILLGNKYTKADDQCRQNYKSICSQY 124 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,280,462 Number of Sequences: 28952 Number of extensions: 265772 Number of successful extensions: 702 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 668 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 701 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1314848736 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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