BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0142 (741 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 62 5e-10 At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 58 6e-09 At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 55 4e-08 At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 54 1e-07 At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 50 2e-06 At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 50 2e-06 At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 48 9e-06 At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 47 1e-05 At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 47 1e-05 At3g55950.1 68416.m06217 protein kinase family protein contains ... 28 5.7 At3g54960.1 68416.m06094 thioredoxin family protein similar to p... 28 5.7 At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 28 7.5 At1g02170.1 68414.m00145 latex-abundant family protein (AMC1) / ... 27 9.9 >At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from [Arabidopsis thaliana] Length = 307 Score = 61.7 bits (143), Expect = 5e-10 Identities = 33/91 (36%), Positives = 45/91 (49%) Frame = +2 Query: 287 FLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVV 466 F + A+W T +F Y GLGIT HR AH+S+K L LL +A Q + Sbjct: 61 FTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPI 116 Query: 467 DWARDHRMHHKYSEPMRTPTTQPEDSSFPTL 559 DW HR HH++++ R P + E F L Sbjct: 117 DWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147 Score = 35.9 bits (79), Expect = 0.028 Identities = 18/55 (32%), Positives = 28/55 (50%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660 Q ++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180 >At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180 GI:2970034 from [Arabidopsis thaliana] Length = 305 Score = 58.0 bits (134), Expect = 6e-09 Identities = 29/89 (32%), Positives = 40/89 (44%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472 F W A+ +Y GLGIT HR AH+S+K L +A Q +DW Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116 Query: 473 ARDHRMHHKYSEPMRTPTTQPEDSSFPTL 559 HR HH++++ R P + E F L Sbjct: 117 VSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145 Score = 35.9 bits (79), Expect = 0.028 Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 2/62 (3%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQ 669 Q +D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYH 183 Query: 670 IL 675 IL Sbjct: 184 IL 185 >At1g06360.1 68414.m00672 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 55.2 bits (127), Expect = 4e-08 Identities = 30/93 (32%), Positives = 40/93 (43%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472 F W F LY + L IT HR AH+S+K L L F A Q +DW Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110 Query: 473 ARDHRMHHKYSEPMRTPTTQPEDSSFPTLAGCC 571 HR HH++++ R P + E F + C Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143 Score = 36.3 bits (80), Expect = 0.021 Identities = 16/45 (35%), Positives = 26/45 (57%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 630 Q +D DPH+ GF+FSH+ W+ ++ + K G +V DL+ Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162 >At3g15850.1 68416.m02005 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 371 Score = 54.0 bits (124), Expect = 1e-07 Identities = 28/78 (35%), Positives = 38/78 (48%), Gaps = 1/78 (1%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 469 F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180 Query: 470 WARDHRMHHKYSEPMRTP 523 W HR HH++ + R P Sbjct: 181 WVSTHRYHHQFCDSDRDP 198 Score = 43.2 bits (97), Expect = 2e-04 Identities = 24/64 (37%), Positives = 33/64 (51%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 675 Q +D DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL Sbjct: 190 QFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-IL 247 Query: 676 MPLA 687 PLA Sbjct: 248 HPLA 251 >At1g06090.1 68414.m00638 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana] Length = 299 Score = 50.0 bits (114), Expect = 2e-06 Identities = 24/77 (31%), Positives = 33/77 (42%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472 F W F + L + + L IT HR HKS+K L A Q +DW Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110 Query: 473 ARDHRMHHKYSEPMRTP 523 HR HH++++ R P Sbjct: 111 VSTHRFHHQFTDSDRDP 127 Score = 36.3 bits (80), Expect = 0.021 Identities = 18/52 (34%), Positives = 26/52 (50%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 651 Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169 >At3g15870.1 68416.m02007 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 292 Score = 49.6 bits (113), Expect = 2e-06 Identities = 24/78 (30%), Positives = 38/78 (48%) Frame = +2 Query: 329 FLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSE 508 +L +G+ IT HR +H+S+ L L +AFQ ++W +HR HHK+ E Sbjct: 57 WLVFINGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCE 116 Query: 509 PMRTPTTQPEDSSFPTLA 562 R P + + F +A Sbjct: 117 TQRDPHSPTQGFWFSHMA 134 Score = 37.5 bits (83), Expect = 0.009 Identities = 17/48 (35%), Positives = 28/48 (58%) Frame = +1 Query: 517 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660 DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++ Sbjct: 120 DPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQR 166 >At1g06350.1 68414.m00671 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 300 Score = 47.6 bits (108), Expect = 9e-06 Identities = 25/86 (29%), Positives = 37/86 (43%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472 F W F + L+ + L IT HR +H+S+K L A Q +DW Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111 Query: 473 ARDHRMHHKYSEPMRTPTTQPEDSSF 550 HR HH++++ R P + E F Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLF 137 Score = 34.3 bits (75), Expect = 0.086 Identities = 17/55 (30%), Positives = 28/55 (50%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660 Q +D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++ Sbjct: 120 QFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173 >At1g06120.1 68414.m00641 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase GB:BAA25180 GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported by cDNA:gi_12083275_gb_AF332434.1_AF332434 Length = 299 Score = 47.2 bits (107), Expect = 1e-05 Identities = 23/77 (29%), Positives = 33/77 (42%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472 F W FA + + + L IT HR H+S+K L A Q +DW Sbjct: 51 FNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDW 110 Query: 473 ARDHRMHHKYSEPMRTP 523 HR HH++++ R P Sbjct: 111 VSTHRFHHQFTDSDRDP 127 Score = 36.3 bits (80), Expect = 0.021 Identities = 18/52 (34%), Positives = 26/52 (50%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 651 Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169 >At1g06100.1 68414.m00639 fatty acid desaturase family protein similar to delta 9 acyl-lipid desaturase (ADS1) GI:2970034 from [Arabidopsis thaliana] Length = 299 Score = 47.2 bits (107), Expect = 1e-05 Identities = 24/77 (31%), Positives = 34/77 (44%) Frame = +2 Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472 F W F I L + + L IT HR H+S+K L +A Q +DW Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110 Query: 473 ARDHRMHHKYSEPMRTP 523 HR HH++++ R P Sbjct: 111 VSIHRFHHQFTDSDRDP 127 Score = 38.3 bits (85), Expect = 0.005 Identities = 20/55 (36%), Positives = 28/55 (50%) Frame = +1 Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660 Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK 172 >At3g55950.1 68416.m06217 protein kinase family protein contains protein kinase domain, Pfam:PF00069; similar to cytokinin-regulated kinase 1 [Nicotiana tabacum] gi|10998537|gb|AAG25966 Length = 814 Score = 28.3 bits (60), Expect = 5.7 Identities = 15/41 (36%), Positives = 18/41 (43%) Frame = +3 Query: 168 PEDAPPHVPPSAEKRPWKIVWRNVILFFICMSVVFMEGTCS 290 P PP PS P K + R ++ F I SV G CS Sbjct: 372 PPPPPPPPSPSTSSPPSKALTRGLLAFAIVGSVGAFAGICS 412 >At3g54960.1 68416.m06094 thioredoxin family protein similar to protein disulfide isomerase GI:5902592 from [Volvox carteri f. nagariensis], GI:2708314 from Chlamydomonas reinhardtii; contains Pfam profile: PF00085 Thioredoxin Length = 579 Score = 28.3 bits (60), Expect = 5.7 Identities = 10/41 (24%), Positives = 21/41 (51%) Frame = -3 Query: 325 GEYRSPPHGFEKEQVPSINTTDMQIKNRITFLHTIFQGLFS 203 GE+ HG+E+E +P ++ D+ + + F + F+ Sbjct: 79 GEFHHGDHGYEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFA 119 >At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Lycopersicon esculentum] GI:7381227; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 468 Score = 27.9 bits (59), Expect = 7.5 Identities = 14/49 (28%), Positives = 28/49 (57%) Frame = +2 Query: 401 ARLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSEPMRTPTTQPEDSS 547 +R P+ + T+ I F +++ + + R+HH+ S+P +P+ P D S Sbjct: 5 SRDPILCITTLILIITF--SLLSYGTEARLHHQASQP-PSPSPNPNDPS 50 Score = 27.9 bits (59), Expect = 7.5 Identities = 11/34 (32%), Positives = 16/34 (47%) Frame = +3 Query: 132 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWR 233 S+ ++ D +P D P P S KR W +R Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 157 >At1g02170.1 68414.m00145 latex-abundant family protein (AMC1) / caspase family protein contains similarity to latex-abundant protein [Hevea brasiliensis] gb:AAD13216; contains Pfam profile PF00656: ICE-like protease (caspase) p20 domain Length = 367 Score = 27.5 bits (58), Expect = 9.9 Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 4/38 (10%) Frame = -2 Query: 581 CFLNN-NQPMWEKKNPRVALW-GSA--SALNICGACDD 480 C +N Q +WE PR LW G+A A++I G CDD Sbjct: 232 CRMNRAGQYVWEDHRPRSGLWKGTAGGEAISISG-CDD 268 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 18,602,714 Number of Sequences: 28952 Number of extensions: 440731 Number of successful extensions: 1267 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 1188 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1260 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1633819784 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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