BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0142
(741 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to ... 62 5e-10
At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to ... 58 6e-09
At1g06360.1 68414.m00672 fatty acid desaturase family protein si... 55 4e-08
At3g15850.1 68416.m02005 fatty acid desaturase family protein si... 54 1e-07
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 50 2e-06
At3g15870.1 68416.m02007 fatty acid desaturase family protein si... 50 2e-06
At1g06350.1 68414.m00671 fatty acid desaturase family protein si... 48 9e-06
At1g06120.1 68414.m00641 fatty acid desaturase family protein si... 47 1e-05
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 47 1e-05
At3g55950.1 68416.m06217 protein kinase family protein contains ... 28 5.7
At3g54960.1 68416.m06094 thioredoxin family protein similar to p... 28 5.7
At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /... 28 7.5
At1g02170.1 68414.m00145 latex-abundant family protein (AMC1) / ... 27 9.9
>At2g31360.1 68415.m03831 delta 9 desaturase (ADS2) identical to
delta 9 acyl-lipid desaturase (ADS2) GI:2970036 from
[Arabidopsis thaliana]
Length = 307
Score = 61.7 bits (143), Expect = 5e-10
Identities = 33/91 (36%), Positives = 45/91 (49%)
Frame = +2
Query: 287 FLFKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVV 466
F + A+W T +F Y GLGIT HR AH+S+K L LL +A Q +
Sbjct: 61 FTWSALWVTFLF----YTIGGLGITVSYHRNLAHRSFKVPKWLEYLLAYCALLAIQGDPI 116
Query: 467 DWARDHRMHHKYSEPMRTPTTQPEDSSFPTL 559
DW HR HH++++ R P + E F L
Sbjct: 117 DWVSTHRYHHQFTDSERDPHSPKEGFWFSHL 147
Score = 35.9 bits (79), Expect = 0.028
Identities = 18/55 (32%), Positives = 28/55 (50%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660
Q ++ DPH+ GF+FSH+ W+ + K G +V DL+ RF +K
Sbjct: 127 QFTDSERDPHSPKEGFWFSHLLWIYDSAYLVSKC-GRRANVEDLKRQWFYRFLQK 180
>At1g06080.1 68414.m00637 delta 9 desaturase (ADS1) identical to
delta 9 acyl-lipid desaturase (ADS1) GB:BAA25180
GI:2970034 from [Arabidopsis thaliana]
Length = 305
Score = 58.0 bits (134), Expect = 6e-09
Identities = 29/89 (32%), Positives = 40/89 (44%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472
F W A+ +Y GLGIT HR AH+S+K L +A Q +DW
Sbjct: 57 FNFTWPALRVALIVYTVGGLGITVSYHRNLAHRSFKVPKWLEYFFAYCGLLAIQGDPIDW 116
Query: 473 ARDHRMHHKYSEPMRTPTTQPEDSSFPTL 559
HR HH++++ R P + E F L
Sbjct: 117 VSTHRYHHQFTDSDRDPHSPNEGFWFSHL 145
Score = 35.9 bits (79), Expect = 0.028
Identities = 20/62 (32%), Positives = 32/62 (51%), Gaps = 2/62 (3%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK--YYQ 669
Q +D DPH+ GF+FSH+ WL + ++ G +V DL+ +F ++ Y
Sbjct: 125 QFTDSDRDPHSPNEGFWFSHLLWLFDTGY-LVEKCGRRTNVEDLKRQWYYKFLQRTVLYH 183
Query: 670 IL 675
IL
Sbjct: 184 IL 185
>At1g06360.1 68414.m00672 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 55.2 bits (127), Expect = 4e-08
Identities = 30/93 (32%), Positives = 40/93 (43%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472
F W F LY + L IT HR AH+S+K L L F A Q +DW
Sbjct: 51 FNYKWEALRFGFVLYALTSLSITFSYHRNLAHRSFKLPKWLEYPLAYFAVFALQGDPLDW 110
Query: 473 ARDHRMHHKYSEPMRTPTTQPEDSSFPTLAGCC 571
HR HH++++ R P + E F + C
Sbjct: 111 VSIHRFHHQFTDSDRDPHSPIEGFWFSHVWWIC 143
Score = 36.3 bits (80), Expect = 0.021
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLR 630
Q +D DPH+ GF+FSH+ W+ ++ + K G +V DL+
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVWWICDTRYIKYKCGGRN-NVMDLK 162
>At3g15850.1 68416.m02005 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 371
Score = 54.0 bits (124), Expect = 1e-07
Identities = 28/78 (35%), Positives = 38/78 (48%), Gaps = 1/78 (1%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGL-GITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVD 469
F+ WR A LY+ +GL GIT HR +HK++K L L A Q +D
Sbjct: 121 FQFNWRAVSVAFGLYIVTGLLGITLSFHRNLSHKAFKLPKWLEYLFAYCGAQALQGNPID 180
Query: 470 WARDHRMHHKYSEPMRTP 523
W HR HH++ + R P
Sbjct: 181 WVSTHRYHHQFCDSDRDP 198
Score = 43.2 bits (97), Expect = 2e-04
Identities = 24/64 (37%), Positives = 33/64 (51%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKKYYQIL 675
Q +D DPH+ GF+FSH+ W + + + G +V DL P RF + Y IL
Sbjct: 190 QFCDSDRDPHSPLDGFWFSHMNW-MFDTNTITQRCGEPNNVGDLEKQPFYRFLRTTY-IL 247
Query: 676 MPLA 687
PLA
Sbjct: 248 HPLA 251
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 50.0 bits (114), Expect = 2e-06
Identities = 24/77 (31%), Positives = 33/77 (42%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472
F W F + L + + L IT HR HKS+K L A Q +DW
Sbjct: 51 FNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 473 ARDHRMHHKYSEPMRTP 523
HR HH++++ R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 36.3 bits (80), Expect = 0.021
Identities = 18/52 (34%), Positives = 26/52 (50%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 651
Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At3g15870.1 68416.m02007 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 292
Score = 49.6 bits (113), Expect = 2e-06
Identities = 24/78 (30%), Positives = 38/78 (48%)
Frame = +2
Query: 329 FLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSE 508
+L +G+ IT HR +H+S+ L L +AFQ ++W +HR HHK+ E
Sbjct: 57 WLVFINGICITLSYHRNLSHRSFDLPKWLEYLFAYGGVLAFQGDPIEWVSNHRYHHKHCE 116
Query: 509 PMRTPTTQPEDSSFPTLA 562
R P + + F +A
Sbjct: 117 TQRDPHSPTQGFWFSHMA 134
Score = 37.5 bits (83), Expect = 0.009
Identities = 17/48 (35%), Positives = 28/48 (58%)
Frame = +1
Query: 517 DPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660
DPH+ T+GF+FSH+ W + ++ G +V+DL P RF ++
Sbjct: 120 DPHSPTQGFWFSHMAW-IFDTSSILENCGGEENVDDLVRQPFYRFLQR 166
>At1g06350.1 68414.m00671 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 300
Score = 47.6 bits (108), Expect = 9e-06
Identities = 25/86 (29%), Positives = 37/86 (43%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472
F W F + L+ + L IT HR +H+S+K L A Q +DW
Sbjct: 52 FNFKWEALRFGLVLFALTTLSITFSFHRNLSHRSFKIPKWLEYPWAYSAVFALQGDPMDW 111
Query: 473 ARDHRMHHKYSEPMRTPTTQPEDSSF 550
HR HH++++ R P + E F
Sbjct: 112 VSIHRFHHQFTDSDRDPHSPKEGLLF 137
Score = 34.3 bits (75), Expect = 0.086
Identities = 17/55 (30%), Positives = 28/55 (50%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660
Q +D DPH+ G FSHI W+ ++ + K G +V DL+ +F ++
Sbjct: 120 QFTDSDRDPHSPKEGLLFSHILWIFDTQYIKYKCGGRD-NVLDLKKQWFYKFLRR 173
>At1g06120.1 68414.m00641 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]; supported
by cDNA:gi_12083275_gb_AF332434.1_AF332434
Length = 299
Score = 47.2 bits (107), Expect = 1e-05
Identities = 23/77 (29%), Positives = 33/77 (42%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472
F W FA + + + L IT HR H+S+K L A Q +DW
Sbjct: 51 FNYTWEAFRFAAMVGISTNLSITFSYHRNLTHRSFKLPKWLEYPFAYSALFALQGHPIDW 110
Query: 473 ARDHRMHHKYSEPMRTP 523
HR HH++++ R P
Sbjct: 111 VSTHRFHHQFTDSDRDP 127
Score = 36.3 bits (80), Expect = 0.021
Identities = 18/52 (34%), Positives = 26/52 (50%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRF 651
Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVFWIFDTSYIREKCGGRD-NVMDLKQQWFYRF 169
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 47.2 bits (107), Expect = 1e-05
Identities = 24/77 (31%), Positives = 34/77 (44%)
Frame = +2
Query: 293 FKAMWRTSIFAIFLYLCSGLGITAGAHRLWAHKSYKARLPLRILLTIFNTIAFQDAVVDW 472
F W F I L + + L IT HR H+S+K L +A Q +DW
Sbjct: 51 FNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEYPFAYSALLALQGDPLDW 110
Query: 473 ARDHRMHHKYSEPMRTP 523
HR HH++++ R P
Sbjct: 111 VSIHRFHHQFTDSDRDP 127
Score = 38.3 bits (85), Expect = 0.005
Identities = 20/55 (36%), Positives = 28/55 (50%)
Frame = +1
Query: 496 QIFRADADPHNATRGFFFSHIGWLLLRKHPEIKAKGHTVDVNDLRNDPILRFQKK 660
Q +D DPH+ GF+FSH+ W+ + K G +V DL+ RF KK
Sbjct: 119 QFTDSDRDPHSPIEGFWFSHVLWIFDTDYIREKC-GRRNNVMDLKQQWFYRFLKK 172
>At3g55950.1 68416.m06217 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
cytokinin-regulated kinase 1 [Nicotiana tabacum]
gi|10998537|gb|AAG25966
Length = 814
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/41 (36%), Positives = 18/41 (43%)
Frame = +3
Query: 168 PEDAPPHVPPSAEKRPWKIVWRNVILFFICMSVVFMEGTCS 290
P PP PS P K + R ++ F I SV G CS
Sbjct: 372 PPPPPPPPSPSTSSPPSKALTRGLLAFAIVGSVGAFAGICS 412
>At3g54960.1 68416.m06094 thioredoxin family protein similar to
protein disulfide isomerase GI:5902592 from [Volvox
carteri f. nagariensis], GI:2708314 from Chlamydomonas
reinhardtii; contains Pfam profile: PF00085 Thioredoxin
Length = 579
Score = 28.3 bits (60), Expect = 5.7
Identities = 10/41 (24%), Positives = 21/41 (51%)
Frame = -3
Query: 325 GEYRSPPHGFEKEQVPSINTTDMQIKNRITFLHTIFQGLFS 203
GE+ HG+E+E +P ++ D+ + + F + F+
Sbjct: 79 GEFHHGDHGYEEEPLPPVDEKDVAVLTKDNFTEFVGNNSFA 119
>At4g01890.1 68417.m00247 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase [Lycopersicon esculentum] GI:7381227;
contains PF00295: Glycosyl hydrolases family 28
(polygalacturonases)
Length = 468
Score = 27.9 bits (59), Expect = 7.5
Identities = 14/49 (28%), Positives = 28/49 (57%)
Frame = +2
Query: 401 ARLPLRILLTIFNTIAFQDAVVDWARDHRMHHKYSEPMRTPTTQPEDSS 547
+R P+ + T+ I F +++ + + R+HH+ S+P +P+ P D S
Sbjct: 5 SRDPILCITTLILIITF--SLLSYGTEARLHHQASQP-PSPSPNPNDPS 50
Score = 27.9 bits (59), Expect = 7.5
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = +3
Query: 132 SWVLYEADANNLPEDAPPHVPPSAEKRPWKIVWR 233
S+ ++ D +P D P P S KR W +R
Sbjct: 124 SYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYR 157
>At1g02170.1 68414.m00145 latex-abundant family protein (AMC1) /
caspase family protein contains similarity to
latex-abundant protein [Hevea brasiliensis] gb:AAD13216;
contains Pfam profile PF00656: ICE-like protease
(caspase) p20 domain
Length = 367
Score = 27.5 bits (58), Expect = 9.9
Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Frame = -2
Query: 581 CFLNN-NQPMWEKKNPRVALW-GSA--SALNICGACDD 480
C +N Q +WE PR LW G+A A++I G CDD
Sbjct: 232 CRMNRAGQYVWEDHRPRSGLWKGTAGGEAISISG-CDD 268
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,602,714
Number of Sequences: 28952
Number of extensions: 440731
Number of successful extensions: 1267
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 1188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1260
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1633819784
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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