Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0140
(721 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g28770.1 68416.m03591 expressed protein 41 0.001
At1g45545.1 68414.m05199 hypothetical protein contains Pfam prof... 33 0.19
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 33 0.25
At1g52360.1 68414.m05909 coatomer protein complex, subunit beta ... 32 0.44
At3g30820.1 68416.m03953 hypothetical protein 30 1.3
At5g58440.1 68418.m07319 phox (PX) domain-containing protein sim... 29 2.3
At5g60530.1 68418.m07590 late embryogenesis abundant protein-rel... 29 3.1
At3g01770.1 68416.m00116 DNA-binding bromodomain-containing prot... 29 3.1
At5g52800.1 68418.m06552 expressed protein ; expression supporte... 29 4.1
At1g44910.1 68414.m05146 FF domain-containing protein / WW domai... 29 4.1
At5g48610.1 68418.m06012 expressed protein ; expression supporte... 28 5.4
At4g35510.1 68417.m05048 expressed protein 28 5.4
At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA li... 28 5.4
At4g12770.1 68417.m02004 auxilin-related low similarity to SP|Q2... 28 7.2
At3g13460.2 68416.m01694 expressed protein contains Pfam profile... 28 7.2
At3g13460.1 68416.m01693 expressed protein contains Pfam profile... 28 7.2
At2g15860.1 68415.m01818 expressed protein and genefinder 28 7.2
At2g02480.1 68415.m00187 DNA polymerase-related weak similarity ... 28 7.2
At3g59080.1 68416.m06586 aspartyl protease family protein contai... 27 9.5
At2g28650.1 68415.m03483 exocyst subunit EXO70 family protein co... 27 9.5
At1g69760.1 68414.m08027 expressed protein 27 9.5
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 40.7 bits (91), Expect = 0.001
Identities = 34/149 (22%), Positives = 70/149 (46%), Gaps = 1/149 (0%)
Frame = +1
Query: 91 VRKQKERKQFTYKMNTQKC-EQNKEAKEKTFVEKQAERMQRLRNLHTARNEARTQNTKKS 267
++KQ++ K+ T K K E+NK+ KEK E A + + + +++ + + K
Sbjct: 971 LKKQEDNKKETTKSENSKLKEENKDNKEKKESEDSASKNREKKEYEEKKSKTKEEAKK-- 1028
Query: 268 *QKRLGTSYQRTMKLNGARQSG**RIRRNEKKQKQKAKTTIESNC*IFLR*KQSDSNVKK 447
+K+ +R K + R+S + + K K+K + T E K+S+++ K
Sbjct: 1029 -EKKKSQDKKREEKDSEERKSKKEKEESRDLKAKKKEEETKEK--------KESENHKSK 1079
Query: 448 RKRTQTKGSRRMNMPQYDNTKVSEKHATS 534
+K + + +M + ++ K +KH S
Sbjct: 1080 KKEDKKEHEDNKSMKKEEDKKEKKKHEES 1108
>At1g45545.1 68414.m05199 hypothetical protein contains Pfam profile
PF05701: Plant protein of unknown function (DUF827)
Length = 752
Score = 33.1 bits (72), Expect = 0.19
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = +3
Query: 246 DTKHQEVVAEEARNKLPTNYEAKRRQAEWLVEDQKKREEAETEGKNYDRVKLLNISAIE 422
D K Q VVAEEA++++ E R E L + +K E+ E + K + L + +E
Sbjct: 204 DYKKQAVVAEEAKHQVVMELERTRNVVEELKLELEKAEKEEQQAKQDSDLAKLRVEEME 262
>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
RNA helicase [Rattus norvegicus] GI:897915; contains
Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1166
Score = 32.7 bits (71), Expect = 0.25
Identities = 16/45 (35%), Positives = 27/45 (60%)
Frame = +3
Query: 246 DTKHQEVVAEEARNKLPTNYEAKRRQAEWLVEDQKKREEAETEGK 380
+ K +E E+ + KL E +RR+ + E ++K+EEAE+E K
Sbjct: 260 EKKTREEELEDEQKKLDEEVEKRRRRVQEWQELKRKKEEAESESK 304
>At1g52360.1 68414.m05909 coatomer protein complex, subunit beta 2
(beta prime), putative contains 7 WD-40 repeats (PF00400)
(1 weak); similar to (SP:O55029) Coatomer beta' subunit
(Beta'-coat protein) (Beta'-COP) (p102) (SP:O55029) [Mus
musculus]; similar to GI:298096 from [Homo sapiens]
Length = 926
Score = 31.9 bits (69), Expect = 0.44
Identities = 19/61 (31%), Positives = 28/61 (45%)
Frame = +3
Query: 246 DTKHQEVVAEEARNKLPTNYEAKRRQAEWLVEDQKKREEAETEGKNYDRVKLLNISAIEA 425
D H+EVVAEE N+ + E E + + EE +G + D L+N S +
Sbjct: 855 DMDHEEVVAEENGNEQRNEDDVAEHVEEHHEEKEAEEEEGIVDGDSTDGAVLVNGSEADE 914
Query: 426 E 428
E
Sbjct: 915 E 915
>At3g30820.1 68416.m03953 hypothetical protein
Length = 405
Score = 30.3 bits (65), Expect = 1.3
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = +2
Query: 224 TPPETKLGHKTPRSRSRRG*EQATNE 301
TPPE LGH+ RSR + G EQ+++E
Sbjct: 375 TPPEQSLGHRLGRSRRQPG-EQSSSE 399
>At5g58440.1 68418.m07319 phox (PX) domain-containing protein
similar to SP|O60749 Sorting nexin 2 {Homo sapiens};
contains Pfam profile PF00787: PX domain
Length = 587
Score = 29.5 bits (63), Expect = 2.3
Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 1/68 (1%)
Frame = +3
Query: 273 EEARNKLPTNYEAKRRQAEWLVEDQKKREEAETEG-KNYDRVKLLNISAIEAERLERXXX 449
E A +K+ +++ R+ E L E K E+A+ K Y+R+K N S +E ER
Sbjct: 488 EAASSKVFGGDKSRIRKIEELKETIKVTEDAKNVAIKGYERIKENNRSEVERLDRERRAD 547
Query: 450 XXNPDQGF 473
N +GF
Sbjct: 548 FMNMMKGF 555
>At5g60530.1 68418.m07590 late embryogenesis abundant
protein-related / LEA protein-related similar to late
embryogenesis abundant protein [Picea glauca] GI:1350543
Length = 439
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +1
Query: 94 RKQKERKQFTYKMNTQKCEQNKEAKEKTFVEKQAERMQR 210
+K KERK+ K +K +++KE KEK EK+ + ++
Sbjct: 88 KKDKERKEKEKKDKLEKEKKDKERKEKERKEKERKAKEK 126
>At3g01770.1 68416.m00116 DNA-binding bromodomain-containing protein
contains bromodomain, INTERPRO:IPR001487
Length = 620
Score = 29.1 bits (62), Expect = 3.1
Identities = 14/49 (28%), Positives = 23/49 (46%)
Frame = +1
Query: 100 QKERKQFTYKMNTQKCEQNKEAKEKTFVEKQAERMQRLRNLHTARNEAR 246
Q+E+++ + +K EAKE ++AE + R L R AR
Sbjct: 481 QREKEELELQKKKEKARLQAEAKEAEEARRKAEAQEAKRKLELEREAAR 529
>At5g52800.1 68418.m06552 expressed protein ; expression supported
by MPSS
Length = 618
Score = 28.7 bits (61), Expect = 4.1
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -3
Query: 227 VCKLRSLCMRSACFSTNVFS 168
VCK+ S CM++ CF T V S
Sbjct: 370 VCKMESDCMKTLCFDTEVNS 389
>At1g44910.1 68414.m05146 FF domain-containing protein / WW
domain-containing protein contains Pfam profiles PF01846:
FF domain, PF00397: WW domain
Length = 946
Score = 28.7 bits (61), Expect = 4.1
Identities = 33/144 (22%), Positives = 59/144 (40%), Gaps = 1/144 (0%)
Frame = +1
Query: 91 VRKQKERKQFTYKMNTQKCEQNKEAKEKTFVEKQAERMQRLR-NLHTARNEARTQNTKKS 267
VRK+KER + + + K + KE + + EK ER +R + TA + + +K
Sbjct: 806 VRKEKERDEKEKRKDKDKERREKEREREK--EKGKERSKREESDGETAMDVSEGHKDEKR 863
Query: 268 *QKRLGTSYQRTMKLNGARQSG**RIRRNEKKQKQKAKTTIESNC*IFLR*KQSDSNVKK 447
K ++R N R R+E K+ + +S+S +
Sbjct: 864 KGKDRDRKHRRRHHNNSDEDVSSDRDDRDESKKSSRKHGNDRKKSRKHANSPESESE-NR 922
Query: 448 RKRTQTKGSRRMNMPQYDNTKVSE 519
KR + + SRR + ++ +V E
Sbjct: 923 HKRQKKESSRRSGNDELEDGEVGE 946
>At5g48610.1 68418.m06012 expressed protein ; expression supported
by MPSS
Length = 470
Score = 28.3 bits (60), Expect = 5.4
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +1
Query: 97 KQKERKQFTYKMNTQKCEQNKEAKEKTFVEKQAE 198
KQKERK+ K QK ++ + K K EK+AE
Sbjct: 41 KQKERKEKKDKHKDQKDKEKGKEKGKPLEEKKAE 74
>At4g35510.1 68417.m05048 expressed protein
Length = 333
Score = 28.3 bits (60), Expect = 5.4
Identities = 18/74 (24%), Positives = 33/74 (44%)
Frame = +2
Query: 494 NTTIQRLVKNMPPADMERYEKQKQKYGEAFYGGPNVIIHGMHEDHKEAVDKWLMIWKVRL 673
NT+ R+++ + +RY+KQK+ G ++I+ G +D VD + +
Sbjct: 179 NTSYPRVLRTSEE-NGQRYKKQKKNKSVVEGGSSSIIVTGSMKDIAGVVDSDSSYEGLEI 237
Query: 674 QRELNTRGDEFIMM 715
R D +MM
Sbjct: 238 ARSNRDEADSMLMM 251
>At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA
ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA
synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS)
{Arabidopsis thaliana}
Length = 1108
Score = 28.3 bits (60), Expect = 5.4
Identities = 17/59 (28%), Positives = 32/59 (54%)
Frame = +1
Query: 100 QKERKQFTYKMNTQKCEQNKEAKEKTFVEKQAERMQRLRNLHTARNEARTQNTKKS*QK 276
+ E+K T + +K ++ ++AKEK +++A +RL L + + T KKS +K
Sbjct: 47 ESEKKILTEEELERKKKKEEKAKEKELKKQKALEKERLAELKAKQAKDGTNVPKKSAKK 105
>At4g12770.1 68417.m02004 auxilin-related low similarity to
SP|Q27974 Auxilin {Bos taurus}
Length = 909
Score = 27.9 bits (59), Expect = 7.2
Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
Frame = +1
Query: 91 VRKQKERKQFTYKMN-TQKCEQNKEAKEKTFVEKQAERMQRLRNLHTARNEAR 246
VR +ER + +M TQ E+ + +E+ +E++ ER+ + + A EAR
Sbjct: 507 VRLDRERAEREAEMEKTQAREREEREREQKRIERERERLLARQAVERATREAR 559
>At3g13460.2 68416.m01694 expressed protein contains Pfam profile
PF04146: YT521-B-like family
Length = 664
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -2
Query: 510 LCIVVLWHVHTSRTLGLGSFSFFYVRVALLLSQK 409
L IV ++ H+S+T L FSF+ VR +L +K
Sbjct: 570 LKIVKIFKEHSSKTCILDDFSFYEVRQKTILEKK 603
>At3g13460.1 68416.m01693 expressed protein contains Pfam profile
PF04146: YT521-B-like family
Length = 667
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -2
Query: 510 LCIVVLWHVHTSRTLGLGSFSFFYVRVALLLSQK 409
L IV ++ H+S+T L FSF+ VR +L +K
Sbjct: 573 LKIVKIFKEHSSKTCILDDFSFYEVRQKTILEKK 606
>At2g15860.1 68415.m01818 expressed protein and genefinder
Length = 512
Score = 27.9 bits (59), Expect = 7.2
Identities = 22/79 (27%), Positives = 37/79 (46%)
Frame = +3
Query: 249 TKHQEVVAEEARNKLPTNYEAKRRQAEWLVEDQKKREEAETEGKNYDRVKLLNISAIEAE 428
+++ VAE+A + E +E ++++K EEA+TE + D L SA+ E
Sbjct: 66 SRNAAAVAEKAAKSIAEMGEVDE-DSESSAKEEEKTEEADTEQDSDDENAKLKKSAL--E 122
Query: 429 RLERXXXXXNPDQGFSTYE 485
RLE QG ++
Sbjct: 123 RLEGASEESLLSQGLKVFD 141
>At2g02480.1 68415.m00187 DNA polymerase-related weak similarity to
DNA polymerase III holoenzyme tau subunit [Thermus
thermophilus] GI:2583049
Length = 1218
Score = 27.9 bits (59), Expect = 7.2
Identities = 13/35 (37%), Positives = 17/35 (48%)
Frame = +1
Query: 94 RKQKERKQFTYKMNTQKCEQNKEAKEKTFVEKQAE 198
R+ KE+K F Y TQK K K E++ E
Sbjct: 74 RRGKEKKVFLYNWKTQKSSSEKSGLAKNGKEEEEE 108
>At3g59080.1 68416.m06586 aspartyl protease family protein contains
similarity to CND41, chloroplast nucleoid DNA binding
protein (GI:2541876) [Nicotiana tabacum]; contains Pfam
profile PF00026: Eukaryotic aspartyl protease
Length = 535
Score = 27.5 bits (58), Expect = 9.5
Identities = 14/58 (24%), Positives = 26/58 (44%)
Frame = +1
Query: 106 ERKQFTYKMNTQKCEQNKEAKEKTFVEKQAERMQRLRNLHTARNEARTQNTKKS*QKR 279
E K + + ++ ++A + +E Q + R++ LH E QNT QK+
Sbjct: 76 ENKTVKFHLKRRETTTTEKATTNSVLELQIRDLTRIQTLHKRVLEKNNQNTVSQKQKK 133
>At2g28650.1 68415.m03483 exocyst subunit EXO70 family protein
contains Pfam domain PF03081: Exo70 exocyst complex
subunit
Length = 573
Score = 27.5 bits (58), Expect = 9.5
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +3
Query: 264 VVAEEARNKLPTNYE-AKRRQAEWLVEDQKKREE 362
+ AEE + +E A +RQ W+V D K R+E
Sbjct: 478 MTAEEGIKRFSDGFEEAYKRQTGWIVPDSKLRDE 511
>At1g69760.1 68414.m08027 expressed protein
Length = 177
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/54 (27%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +3
Query: 204 AETA*LTHRPKRS*DTKHQEVV--AEEARNKLPTNYEAKRRQAEWLVEDQKKRE 359
AET +T KR D + QE++ EE + + P++ + Q L++D+ + +
Sbjct: 2 AETTTVTSSLKRQRDEQEQEIIKTQEETKRQKPSSSSSSYNQVLTLLKDEAEND 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,422,655
Number of Sequences: 28952
Number of extensions: 283418
Number of successful extensions: 1019
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 957
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1013
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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