BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0135 (781 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g60380.1 68416.m06753 expressed protein 33 0.28 At1g44910.1 68414.m05146 FF domain-containing protein / WW domai... 31 0.86 At4g24260.1 68417.m03481 endo-1,4-beta-glucanase, putative / cel... 29 3.5 At4g03260.1 68417.m00445 leucine-rich repeat family protein cont... 29 3.5 At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family... 29 3.5 At5g47750.1 68418.m05899 protein kinase, putative similar to pro... 29 4.6 At3g03060.1 68416.m00302 AAA-type ATPase family protein contains... 29 4.6 At5g19960.1 68418.m02376 RNA recognition motif (RRM)-containing ... 28 6.0 At5g02070.1 68418.m00128 protein kinase-related contains eukaryo... 28 6.0 At2g01100.3 68415.m00018 expressed protein 28 6.0 At2g01100.2 68415.m00017 expressed protein 28 6.0 At2g01100.1 68415.m00016 expressed protein 28 6.0 At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 28 6.0 At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR... 28 6.0 At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 28 6.0 At5g03040.1 68418.m00252 calmodulin-binding family protein simil... 28 8.0 At4g03550.1 68417.m00486 glycosyl transferase family 48 protein ... 28 8.0 At3g12640.1 68416.m01573 RNA recognition motif (RRM)-containing ... 28 8.0 >At3g60380.1 68416.m06753 expressed protein Length = 743 Score = 32.7 bits (71), Expect = 0.28 Identities = 19/75 (25%), Positives = 33/75 (44%), Gaps = 2/75 (2%) Frame = +1 Query: 229 HGREVYGQFTSLKTSPQTQFESHSRPDLRSPQQLEREIIRQRGLVEGRRAASHPH--LLD 402 HG++V S + + + ES R R P+Q E I+ + RA S P ++ Sbjct: 611 HGKDVKTDGDSSEDRAEAKVESRGRTKSRRPRQEELSIVLHQEKSSETRAKSEPEEVAME 670 Query: 403 EPQSRPEASTPYSHD 447 EPQ+ + + + Sbjct: 671 EPQAEQQPEVTFEEE 685 >At1g44910.1 68414.m05146 FF domain-containing protein / WW domain-containing protein contains Pfam profiles PF01846: FF domain, PF00397: WW domain Length = 946 Score = 31.1 bits (67), Expect = 0.86 Identities = 15/49 (30%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +1 Query: 400 DEPQSRPEASTPYSHDRSRRNSHANLLEATEENLH-RTMKENRMMEGSE 543 D+ ++S + +DR + HAN E+ EN H R KE+ G++ Sbjct: 889 DDRDESKKSSRKHGNDRKKSRKHANSPESESENRHKRQKKESSRRSGND 937 >At4g24260.1 68417.m03481 endo-1,4-beta-glucanase, putative / cellulase, putative similar to endo-1,4-beta-D-glucanase; cellulase GI:5689613 from [Brassica napus] Length = 620 Score = 29.1 bits (62), Expect = 3.5 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +3 Query: 126 CWRRPIDPNLRRLVPTPYTSSTDLRDEEA 212 CW RP D + +R V Y+S +DL E A Sbjct: 247 CWMRPEDIHYKRTVSQCYSSCSDLAAEMA 275 >At4g03260.1 68417.m00445 leucine-rich repeat family protein contains leucine rich repeat (LRR) domains, Pfam:PF00560 Length = 677 Score = 29.1 bits (62), Expect = 3.5 Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 5/74 (6%) Frame = +1 Query: 373 RAASHPHLLDEPQSRPEASTPYS----HDRSRRNSHANLLEATEENLHRTMKENRMMEGS 540 R+ S P++ D S E S+P+ H RS + HA T++++H T +E + E Sbjct: 207 RSNSLPNIAD---SSSEKSSPFKYSSHHSRSSDDLHALDTRQTDKSVHETDEEVKQEEDQ 263 Query: 541 ELD-*LHKGGQVSK 579 + D +H G +K Sbjct: 264 DRDYDMHNSGDNNK 277 >At2g35640.1 68415.m04371 hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO:IPR002965 Length = 340 Score = 29.1 bits (62), Expect = 3.5 Identities = 15/43 (34%), Positives = 21/43 (48%), Gaps = 1/43 (2%) Frame = +1 Query: 103 PPSQIAGDVGVAPSTPTSGAWFRRPI-PAVRTSGTKKRGVPTG 228 PP ++ + P P S ++ PI P V TS TK+R G Sbjct: 199 PPQSLSLSLPSPPQPPPSSSFHAEPIPPTVGTSSTKRRRTTPG 241 >At5g47750.1 68418.m05899 protein kinase, putative similar to protein kinase G11A [Oryza sativa] SWISS-PROT:P47997 Length = 586 Score = 28.7 bits (61), Expect = 4.6 Identities = 25/94 (26%), Positives = 36/94 (38%), Gaps = 2/94 (2%) Frame = +1 Query: 220 PTGHGREVYGQFTSLKTSPQTQFESHSRPDLRSPQQLEREIIRQR--GLVEGRRAASHPH 393 P+GHG EV Q K S ++ + P QL+R + R G+ E +R P Sbjct: 7 PSGHGAEVEAQ----KRSSSNSSTKSAKAETFEPMQLQRSVTNPRAVGIPESKRL---PE 59 Query: 394 LLDEPQSRPEASTPYSHDRSRRNSHANLLEATEE 495 + S P P S H + L E+ Sbjct: 60 SFRKRSSDPAVCKPDFSSLSTVLEHVDSLTIDEK 93 >At3g03060.1 68416.m00302 AAA-type ATPase family protein contains a ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017 Length = 639 Score = 28.7 bits (61), Expect = 4.6 Identities = 33/163 (20%), Positives = 63/163 (38%), Gaps = 8/163 (4%) Frame = +1 Query: 82 PTKDATEPPSQIAGDVGVAP-----STPTSGAWFRRPIPAVRTSGTKKRGVPTGHGREVY 246 P + A+ PP +AG+ AP P + + P R + K + + ++V+ Sbjct: 42 PQQQASPPPPSLAGEESSAPPRARNDNPRTSSGGFDPEALERGAKALKEINHSSYAKKVF 101 Query: 247 GQFTSLKTSPQTQFESHSRPDLRSPQQLEREIIRQRGLV-EGRRAASHPHLLDEPQSRPE 423 + + QT+F + ++ Q E E R + + E ++ A H +R E Sbjct: 102 ESIKQQEETKQTEFATKAQEFKAMQAQAETE--RHKVIYDEQKKLAQHQAQTKSQMARYE 159 Query: 424 ASTPYSHDRSRRNSHANLLEATEENLHRTMKEN--RMMEGSEL 546 ++ ++S E HRT + +M E S + Sbjct: 160 DDLARKRMQACKSS-KQFFNTAENEFHRTRNQELVKMQEDSAI 201 >At5g19960.1 68418.m02376 RNA recognition motif (RRM)-containing protein low similarity to glycine-rich RNA-binding protein [Euphorbia esula] GI:2645699; contains INTERPRO:IPR000504 RNA-binding region RNP-1 (RNA recognition motif) (RRM) domain Length = 337 Score = 28.3 bits (60), Expect = 6.0 Identities = 32/124 (25%), Positives = 49/124 (39%) Frame = +1 Query: 304 PDLRSPQQLEREIIRQRGLVEGRRAASHPHLLDEPQSRPEASTPYSHDRSRRNSHANLLE 483 PD RS ER+ R R S D E + +SHD RRN H Sbjct: 106 PDRRSDGNYERDRYSDRSRERDR---SQDRRKDHRYIEKERAYEHSHDFERRNDH----- 157 Query: 484 ATEENLHRTMKENRMMEGSELD*LHKGGQVSKIESGRVVDL*NLSEYTKMYTEERLTAII 663 + + R + R+ +G E D G S +++GR ++ + E T+ + I+ Sbjct: 158 ---DMVDRNGYKERVFDGDEGD---WRGDRSYVDNGRGINGTSAHEGRSQETKREDSTIL 211 Query: 664 VGRR 675 G R Sbjct: 212 DGGR 215 >At5g02070.1 68418.m00128 protein kinase-related contains eukaryotic protein kinase domain, INTERPRO:IPR000719 Length = 657 Score = 28.3 bits (60), Expect = 6.0 Identities = 13/41 (31%), Positives = 25/41 (60%), Gaps = 2/41 (4%) Frame = +1 Query: 430 TPYSHDRSRRNSHANLLEATEENL--HRTMKENRMMEGSEL 546 T +SH + +++ H N+++ EE L + T K +R+ G E+ Sbjct: 316 TKHSHQKVKKDIHKNIVKEREEMLSANSTGKSSRIFTGREI 356 >At2g01100.3 68415.m00018 expressed protein Length = 247 Score = 28.3 bits (60), Expect = 6.0 Identities = 14/65 (21%), Positives = 32/65 (49%) Frame = +1 Query: 331 EREIIRQRGLVEGRRAASHPHLLDEPQSRPEASTPYSHDRSRRNSHANLLEATEENLHRT 510 E ++ R +G +RAAS + S + SH++ RR++H++ ++ ++ Sbjct: 42 ETKVKRSKGPKRKQRAASESSSESDSSSEVRRKSRRSHNKHRRHAHSDSDDSDRRKEKKS 101 Query: 511 MKENR 525 ++ R Sbjct: 102 RRQKR 106 >At2g01100.2 68415.m00017 expressed protein Length = 247 Score = 28.3 bits (60), Expect = 6.0 Identities = 14/65 (21%), Positives = 32/65 (49%) Frame = +1 Query: 331 EREIIRQRGLVEGRRAASHPHLLDEPQSRPEASTPYSHDRSRRNSHANLLEATEENLHRT 510 E ++ R +G +RAAS + S + SH++ RR++H++ ++ ++ Sbjct: 42 ETKVKRSKGPKRKQRAASESSSESDSSSEVRRKSRRSHNKHRRHAHSDSDDSDRRKEKKS 101 Query: 511 MKENR 525 ++ R Sbjct: 102 RRQKR 106 >At2g01100.1 68415.m00016 expressed protein Length = 247 Score = 28.3 bits (60), Expect = 6.0 Identities = 14/65 (21%), Positives = 32/65 (49%) Frame = +1 Query: 331 EREIIRQRGLVEGRRAASHPHLLDEPQSRPEASTPYSHDRSRRNSHANLLEATEENLHRT 510 E ++ R +G +RAAS + S + SH++ RR++H++ ++ ++ Sbjct: 42 ETKVKRSKGPKRKQRAASESSSESDSSSEVRRKSRRSHNKHRRHAHSDSDDSDRRKEKKS 101 Query: 511 MKENR 525 ++ R Sbjct: 102 RRQKR 106 >At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1 protein identical to SP|O22315 Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis thaliana} Length = 303 Score = 28.3 bits (60), Expect = 6.0 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Frame = +3 Query: 543 ARLAAQGRSSFEDRIRTSGRSIESQRIHENVYRRTPDS-HNRRTP 674 +R ++GRS + R R+ GRS+ R R+P + +RR+P Sbjct: 201 SRSPSRGRSYSKSRSRSRGRSVSRSRSRSRSRSRSPKAKSSRRSP 245 >At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR1 protein identical to SP|O22315 Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis thaliana} Length = 285 Score = 28.3 bits (60), Expect = 6.0 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Frame = +3 Query: 543 ARLAAQGRSSFEDRIRTSGRSIESQRIHENVYRRTPDS-HNRRTP 674 +R ++GRS + R R+ GRS+ R R+P + +RR+P Sbjct: 201 SRSPSRGRSYSKSRSRSRGRSVSRSRSRSRSRSRSPKAKSSRRSP 245 >At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1 protein identical to SP|O22315 Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis thaliana} Length = 303 Score = 28.3 bits (60), Expect = 6.0 Identities = 15/45 (33%), Positives = 25/45 (55%), Gaps = 1/45 (2%) Frame = +3 Query: 543 ARLAAQGRSSFEDRIRTSGRSIESQRIHENVYRRTPDS-HNRRTP 674 +R ++GRS + R R+ GRS+ R R+P + +RR+P Sbjct: 201 SRSPSRGRSYSKSRSRSRGRSVSRSRSRSRSRSRSPKAKSSRRSP 245 >At5g03040.1 68418.m00252 calmodulin-binding family protein similar to SF16 protein [Helianthus annuus] GI:560150; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 291 Score = 27.9 bits (59), Expect = 8.0 Identities = 45/176 (25%), Positives = 72/176 (40%), Gaps = 2/176 (1%) Frame = +1 Query: 178 IPAVRTSGTKKRGVPTGHGREVYGQFTSLKTSPQTQFESH-SRPDLRSPQQLEREIIRQR 354 +P+ G +R PT + + ++ QT F + +R LR+ + L R + Sbjct: 91 VPSSSAPGVVRRATPTRFAGKSNEEAAAILI--QTIFRGYLARRALRAMRGLVRLKLLME 148 Query: 355 GLVEGRRAASHPHLLDEPQSRPEASTPYSHDRSRRNSHANLLEATEENLHRTMKENRMME 534 G V R+AA+ + + SR + S R+RR + +EEN R K+ Sbjct: 149 GSVVKRQAANTLKCM-QTLSRVQ-----SQIRARR------IRMSEENQAR-QKQLLQKH 195 Query: 535 GSELD*LHKGGQVS-KIESGRVVDL*NLSEYTKMYTEERLTAIIVGRRIQWDQNAK 699 EL L G + I+S V+ LS+Y ER A + W N+K Sbjct: 196 AKELAGLKNGDNWNDSIQSKEKVEANLLSKYEATMRRERALAYSYSHQQNWKNNSK 251 >At4g03550.1 68417.m00486 glycosyl transferase family 48 protein contains Pfam profile: PF02364 1,3-beta-glucan synthase Length = 1780 Score = 27.9 bits (59), Expect = 8.0 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -2 Query: 315 SKIWS*MRFELCLWTSFQGCELAIDLTSMSRWYTAL 208 S++W+ +L LW S++G LA + M +Y AL Sbjct: 966 SELWTTKLRDLRLWASYRGQTLARTVRGMMYYYRAL 1001 >At3g12640.1 68416.m01573 RNA recognition motif (RRM)-containing protein contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 674 Score = 27.9 bits (59), Expect = 8.0 Identities = 14/51 (27%), Positives = 23/51 (45%) Frame = +3 Query: 603 SIESQRIHENVYRRTPDSHNRRTPDSVGPKREDTLTTGQAANDENASQKSA 755 S E +IH + HN+R+P + T + N++ AS +SA Sbjct: 153 SSEVHKIHNYEKKDHKHRHNKRSPSPQAQSQRKRSRTDDSRNEQRASLRSA 203 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,780,014 Number of Sequences: 28952 Number of extensions: 358014 Number of successful extensions: 1160 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 1117 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1158 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1746037600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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