BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0128
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 125 7e-30
SPBC17D1.02 |||diphthamide biosynthesis protein |Schizosaccharom... 61 2e-10
SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 26 4.4
>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 436
Score = 125 bits (301), Expect = 7e-30
Identities = 56/82 (68%), Positives = 65/82 (79%)
Frame = +2
Query: 263 LCDIIETFTEADTVIMGDVTYGACCIDDFTAVALGVDLLVHYGHSCLIPIDQTSSIKVLY 442
L DI E F + +T++MGDVTYGACCIDDFTA AL D LVHYGHSCLIP+DQT IKVLY
Sbjct: 107 LSDIFEQFCQVETIVMGDVTYGACCIDDFTARALDCDFLVHYGHSCLIPVDQT-PIKVLY 165
Query: 443 IFVDIKIDPSHFIETIKVNFPS 508
+FVDIKID H + ++K N PS
Sbjct: 166 VFVDIKIDLQHVVSSLKHNLPS 187
Score = 64.9 bits (151), Expect = 1e-11
Identities = 29/39 (74%), Positives = 31/39 (79%)
Frame = +3
Query: 138 ACAALPENYNFEIHKTIWRIRSTAAKRVALQMPEGLTMF 254
A LP+NYNFEIHKTIW IR AKRVALQ+PEGL MF
Sbjct: 65 AIKLLPQNYNFEIHKTIWHIRLRKAKRVALQLPEGLLMF 103
Score = 56.8 bits (131), Expect = 3e-09
Identities = 30/61 (49%), Positives = 40/61 (65%), Gaps = 6/61 (9%)
Frame = +1
Query: 490 KSKLSI*THLALVSTIQFVTTLHSVAKNLRIEE------YMVTVPQTKPLSPGEILGCTA 651
K L + LALV TIQFV +L+S+ L+I++ + V +PQ KPLSPGE LGCT+
Sbjct: 182 KHNLPSNSRLALVGTIQFVGSLNSIKDALQIQDEDGKGGFYVVIPQAKPLSPGEALGCTS 241
Query: 652 P 654
P
Sbjct: 242 P 242
>SPBC17D1.02 |||diphthamide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 503
Score = 60.9 bits (141), Expect = 2e-10
Identities = 27/71 (38%), Positives = 41/71 (57%)
Frame = +2
Query: 254 STTLCDIIETFTEADTVIMGDVTYGACCIDDFTAVALGVDLLVHYGHSCLIPIDQTSSIK 433
S + I+ EA+ I+ D YG+CC+D+ A + D +VHYG +CL P TS +
Sbjct: 66 SGKVASILTNLVEANVQILADTNYGSCCVDEVAAEHMSADAIVHYGRACLSP---TSRLP 122
Query: 434 VLYIFVDIKID 466
VLY+F + I+
Sbjct: 123 VLYVFGRLPIN 133
>SPCC622.07 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 128
Score = 26.2 bits (55), Expect = 4.4
Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 5/70 (7%)
Frame = -1
Query: 454 IHKYVQNLNTTSLIYGN*ARVS-----IVHKEVYTECHSSEVIYTAGSVCHISHYHSIGF 290
IH YV+ L YG RV+ I+H+ +Y + Y VC++ +Y+ F
Sbjct: 6 IHLYVKK----DLTYGECVRVAKEKYKIIHRLLYISIIFLFLNYVVDIVCYVKNYNGFSF 61
Query: 289 GECFYYVTEG 260
C+ ++ G
Sbjct: 62 -FCWVFLNLG 70
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,874,044
Number of Sequences: 5004
Number of extensions: 59891
Number of successful extensions: 127
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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