BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0107 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 169 3e-43 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 162 3e-41 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 76 5e-15 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 41 2e-04 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 1.0 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 28 1.0 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 1.8 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.1 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 5.4 SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 25 9.5 SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 25 9.5 SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe... 25 9.5 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 169 bits (411), Expect = 3e-43 Identities = 74/86 (86%), Positives = 80/86 (93%) Frame = +3 Query: 255 DWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAF 434 DWCPTGFK+GI Y+PP VPG +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAF Sbjct: 349 DWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAF 408 Query: 435 VHWYVGEGMEEGEFSEAREDLAALER 512 VHWYVGEGMEEGEFSEAREDLAALER Sbjct: 409 VHWYVGEGMEEGEFSEAREDLAALER 434 Score = 146 bits (353), Expect = 3e-36 Identities = 65/102 (63%), Positives = 78/102 (76%) Frame = +1 Query: 1 VRYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 180 V YPRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LY Sbjct: 264 VPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLY 323 Query: 181 RGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPP 306 RGDV+P+DV AA+ +IK++RTIQFV P + PP Sbjct: 324 RGDVIPRDVQAAVTSIKSRRTIQFVDWC-PTGFKIGICYEPP 364 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 162 bits (394), Expect = 3e-41 Identities = 71/86 (82%), Positives = 79/86 (91%) Frame = +3 Query: 255 DWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAF 434 DWCPTGFK+GI +PP + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAF Sbjct: 345 DWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAF 404 Query: 435 VHWYVGEGMEEGEFSEAREDLAALER 512 VHWYVGEGMEEGEFSEAREDLAALER Sbjct: 405 VHWYVGEGMEEGEFSEAREDLAALER 430 Score = 150 bits (364), Expect = 1e-37 Identities = 68/102 (66%), Positives = 77/102 (75%) Frame = +1 Query: 1 VRYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 180 V YPRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LY Sbjct: 260 VPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLY 319 Query: 181 RGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPP 306 RGDV+P+DV AA+ TIK KRTIQFV P + PP Sbjct: 320 RGDVIPRDVQAAVTTIKAKRTIQFVDWC-PTGFKIGICDRPP 360 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 75.8 bits (178), Expect = 5e-15 Identities = 37/102 (36%), Positives = 60/102 (58%) Frame = +1 Query: 1 VRYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 180 V +PR+HF +V +AP+ + + + +SV E+T F+ N MV DPRHG+Y+ L+ Sbjct: 258 VPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALF 317 Query: 181 RGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPP 306 RG V K+V+ I +++TK + FV + P + + S PP Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWI-PDNVLKAVCSVPP 358 Score = 59.3 bits (137), Expect = 5e-10 Identities = 31/90 (34%), Positives = 50/90 (55%) Frame = +3 Query: 228 QNQAYYPIRDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKF 407 +N AY+ +W P + PP DL + + + N+T+I E + RL +F Sbjct: 336 KNSAYFV--EWIPDNVLKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQF 385 Query: 408 DLMYAKRAFVHWYVGEGMEEGEFSEAREDL 497 M+ ++AF+HWY GEGM+E EF+EA ++ Sbjct: 386 SAMFRRKAFLHWYTGEGMDEMEFTEAESNM 415 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 40.7 bits (91), Expect = 2e-04 Identities = 26/104 (25%), Positives = 49/104 (47%), Gaps = 4/104 (3%) Frame = +1 Query: 10 PRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKYMACCML 177 PR HF L +Y P + E + +V ++ P NQMV +P + +++ + Sbjct: 264 PRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDI 323 Query: 178 YRGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPPW 309 +G+ P DV+ ++ I+ +R F+ P S V+ + P+ Sbjct: 324 IQGEADPADVHKSLLRIRERRYASFIPW-GPASIQVALSKKSPY 366 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.3 bits (60), Expect = 1.0 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = +1 Query: 94 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 219 + ACFEP N C H K C L + KD N ++ Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.3 bits (60), Expect = 1.0 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = -3 Query: 222 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 79 S G +LGY ++ A++V+A + V HL+ G D + K Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.5 bits (58), Expect = 1.8 Identities = 16/39 (41%), Positives = 22/39 (56%) Frame = +2 Query: 11 PVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 127 P S + T +SS+P P N+ PSP S + AS+ P R Sbjct: 1242 PSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.1 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 44 QSSLPRRPTMNSFPSPRSQTHASSPPTR 127 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 5.4 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -2 Query: 199 WVRRHHGTAYSKPCTCHDGG 140 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizosaccharomyces pombe|chr 2|||Manual Length = 953 Score = 25.0 bits (52), Expect = 9.5 Identities = 14/27 (51%), Positives = 16/27 (59%) Frame = +1 Query: 430 LSCTGTSVRVWRRESSPKPVRTWLPSK 510 LS GTS + + SP PVRT L SK Sbjct: 17 LSNNGTSNKAYSVPKSPAPVRTDLFSK 43 >SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 980 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -1 Query: 284 TDLETGWTPVTNWIVRLVLM 225 T +ETGW + WI R+V++ Sbjct: 607 TLMETGWDILFFWIARMVML 626 >SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/37 (32%), Positives = 22/37 (59%) Frame = -3 Query: 114 LEACVCDLGDGKLFMVGLLGRDDWRVRDQWEVDTGVT 4 + A V DL + ++ G+L +D+ ++R WE G+T Sbjct: 352 ITALVEDLLEKEIISDGVLAQDESQLRVLWERREGIT 388 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,776,964 Number of Sequences: 5004 Number of extensions: 55335 Number of successful extensions: 186 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 171 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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