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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0107
         (650 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   169   3e-43
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   162   3e-41
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    76   5e-15
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    41   2e-04
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo...    28   1.0  
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||...    28   1.0  
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual    27   1.8  
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||...    26   4.1  
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    26   5.4  
SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos...    25   9.5  
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos...    25   9.5  
SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces pombe...    25   9.5  

>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  169 bits (411), Expect = 3e-43
 Identities = 74/86 (86%), Positives = 80/86 (93%)
 Frame = +3

Query: 255 DWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAF 434
           DWCPTGFK+GI Y+PP  VPG  +AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAF
Sbjct: 349 DWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAF 408

Query: 435 VHWYVGEGMEEGEFSEAREDLAALER 512
           VHWYVGEGMEEGEFSEAREDLAALER
Sbjct: 409 VHWYVGEGMEEGEFSEAREDLAALER 434



 Score =  146 bits (353), Expect = 3e-36
 Identities = 65/102 (63%), Positives = 78/102 (76%)
 Frame = +1

Query: 1   VRYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 180
           V YPRIHFPLVTY+P++SA KA+HE  SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 264 VPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLY 323

Query: 181 RGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPP 306
           RGDV+P+DV AA+ +IK++RTIQFV    P    +     PP
Sbjct: 324 RGDVIPRDVQAAVTSIKSRRTIQFVDWC-PTGFKIGICYEPP 364


>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  162 bits (394), Expect = 3e-41
 Identities = 71/86 (82%), Positives = 79/86 (91%)
 Frame = +3

Query: 255 DWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAF 434
           DWCPTGFK+GI  +PP  + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFDLMY+KRAF
Sbjct: 345 DWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAF 404

Query: 435 VHWYVGEGMEEGEFSEAREDLAALER 512
           VHWYVGEGMEEGEFSEAREDLAALER
Sbjct: 405 VHWYVGEGMEEGEFSEAREDLAALER 430



 Score =  150 bits (364), Expect = 1e-37
 Identities = 68/102 (66%), Positives = 77/102 (75%)
 Frame = +1

Query: 1   VRYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 180
           V YPRIHFPLVTYAP++SA KA+HE  SV EITN CFEP NQMVKCDPR G+YMA C+LY
Sbjct: 260 VPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLY 319

Query: 181 RGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPP 306
           RGDV+P+DV AA+ TIK KRTIQFV    P    +     PP
Sbjct: 320 RGDVIPRDVQAAVTTIKAKRTIQFVDWC-PTGFKIGICDRPP 360


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 75.8 bits (178), Expect = 5e-15
 Identities = 37/102 (36%), Positives = 60/102 (58%)
 Frame = +1

Query: 1   VRYPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLY 180
           V +PR+HF +V +AP+ +   +  + +SV E+T   F+  N MV  DPRHG+Y+    L+
Sbjct: 258 VPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALF 317

Query: 181 RGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPP 306
           RG V  K+V+  I +++TK +  FV  + P +   +  S PP
Sbjct: 318 RGKVSMKEVDEQIRSVQTKNSAYFVEWI-PDNVLKAVCSVPP 358



 Score = 59.3 bits (137), Expect = 5e-10
 Identities = 31/90 (34%), Positives = 50/90 (55%)
 Frame = +3

Query: 228 QNQAYYPIRDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKF 407
           +N AY+   +W P      +   PP      DL   + +   + N+T+I E + RL  +F
Sbjct: 336 KNSAYFV--EWIPDNVLKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQF 385

Query: 408 DLMYAKRAFVHWYVGEGMEEGEFSEAREDL 497
             M+ ++AF+HWY GEGM+E EF+EA  ++
Sbjct: 386 SAMFRRKAFLHWYTGEGMDEMEFTEAESNM 415


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 26/104 (25%), Positives = 49/104 (47%), Gaps = 4/104 (3%)
 Frame = +1

Query: 10  PRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVKCDP-RHGKYMACCML 177
           PR HF L +Y P  +    E     + +V ++      P NQMV  +P +   +++   +
Sbjct: 264 PRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDI 323

Query: 178 YRGDVVPKDVNAAIATIKTKRTIQFVTGVQPVSRSVSTTSHPPW 309
            +G+  P DV+ ++  I+ +R   F+    P S  V+ +   P+
Sbjct: 324 IQGEADPADVHKSLLRIRERRYASFIPW-GPASIQVALSKKSPY 366


>SPCC18.03 |||shuttle craft like transcriptional
           regulator|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1077

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 14/42 (33%), Positives = 18/42 (42%)
 Frame = +1

Query: 94  ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 219
           +  ACFEP N    C   H K   C  L    +  KD N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401


>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 3227

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 14/48 (29%), Positives = 23/48 (47%)
 Frame = -3

Query: 222 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 79
           S G   +LGY ++     A++V+A + V   HL+ G      D  + K
Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454


>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1611

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 16/39 (41%), Positives = 22/39 (56%)
 Frame = +2

Query: 11   PVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 127
            P S +    T +SS+P  P  N+ PSP S + AS+ P R
Sbjct: 1242 PSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279


>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1158

 Score = 26.2 bits (55), Expect = 4.1
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +2

Query: 44  QSSLPRRPTMNSFPSPRSQTHASSPPTR 127
           + SLPRRP+     +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 25.8 bits (54), Expect = 5.4
 Identities = 10/20 (50%), Positives = 11/20 (55%)
 Frame = -2

Query: 199 WVRRHHGTAYSKPCTCHDGG 140
           W R H    Y+KPC   DGG
Sbjct: 16  WRRDHPFGFYAKPCKSSDGG 35


>SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase
           Ppk30|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 953

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 14/27 (51%), Positives = 16/27 (59%)
 Frame = +1

Query: 430 LSCTGTSVRVWRRESSPKPVRTWLPSK 510
           LS  GTS + +    SP PVRT L SK
Sbjct: 17  LSNNGTSNKAYSVPKSPAPVRTDLFSK 43


>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 980

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = -1

Query: 284 TDLETGWTPVTNWIVRLVLM 225
           T +ETGW  +  WI R+V++
Sbjct: 607 TLMETGWDILFFWIARMVML 626


>SPBC713.03 |||D-lactate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 526

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 12/37 (32%), Positives = 22/37 (59%)
 Frame = -3

Query: 114 LEACVCDLGDGKLFMVGLLGRDDWRVRDQWEVDTGVT 4
           + A V DL + ++   G+L +D+ ++R  WE   G+T
Sbjct: 352 ITALVEDLLEKEIISDGVLAQDESQLRVLWERREGIT 388


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,776,964
Number of Sequences: 5004
Number of extensions: 55335
Number of successful extensions: 186
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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