Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0096
         (816 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...    62   1e-10
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    34   0.028
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom...    31   0.15 
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   0.60 
SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolo...    27   2.4  
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    27   2.4  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   3.2  
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    26   5.6  
SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr...    25   9.7  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 27/57 (47%), Positives = 38/57 (66%)
 Frame = +1

Query: 283 KQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 453
           + K+  +SW PD A +K KM+YSSS D L+++  G+   IQATD SE + E V EK+
Sbjct: 78  RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134



 Score = 52.4 bits (120), Expect = 7e-08
 Identities = 27/71 (38%), Positives = 40/71 (56%)
 Frame = +2

Query: 35  SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 214
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + +++ FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPE---K 60

Query: 215 ECRYGLFDFEY 247
           +CRY ++DFE+
Sbjct: 61  DCRYAIYDFEF 71


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 33.9 bits (74), Expect = 0.028
 Identities = 15/27 (55%), Positives = 21/27 (77%)
 Frame = +1

Query: 277 SKKQKLFLMSWCPDTAKVKKKMLYSSS 357
           SKK  L L+S+ P+ A V++KMLY+SS
Sbjct: 76  SKKNLLQLISYVPENANVRRKMLYASS 102



 Score = 28.7 bits (61), Expect = 1.0
 Identities = 16/56 (28%), Positives = 32/56 (57%), Gaps = 5/56 (8%)
 Frame = +1

Query: 289 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAV 441
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEI 299


>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 447

 Score = 31.5 bits (68), Expect = 0.15
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = +2

Query: 458 PPIANKQHLHTSSRRNRPALRHSCPDDTRPRHH*PLSIITKEN 586
           PP++ K   + S   N P +RHS     RP+ H   S +T+++
Sbjct: 37  PPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFHRADSTVTEQS 79


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 0.60
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 265 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 444
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 445 EKL 453
           E L
Sbjct: 138 EFL 140


>SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolog
           Aif1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 575

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 8/14 (57%), Positives = 10/14 (71%)
 Frame = -2

Query: 263 PWHGACIQSQTGHI 222
           PWHGAC  + TG +
Sbjct: 82  PWHGACFNAATGDV 95


>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 27.5 bits (58), Expect = 2.4
 Identities = 22/81 (27%), Positives = 32/81 (39%)
 Frame = +3

Query: 402 PSDRPLGSVSGGRRREAPRHRSPINSIYTRARDETDPLSDTPALTTRGHDTTSRLVLLQR 581
           P   P  S  G RR  + R R P      R R+E         L  R      RL L  R
Sbjct: 190 PPAEPAESPFGKRRTNSGRFRDPARDPSDRVREEPREWVRRGPLPPRESSERPRLNLKPR 249

Query: 582 KTNSINMIDFTGGRTSCESAR 644
            ++++N  + T   T+  S++
Sbjct: 250 SSSNVN-TEATPSATTTTSSK 269


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +1

Query: 355 SFDALKKSLVGVQKYIQATDLSEASQ 432
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 314

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 20/74 (27%), Positives = 30/74 (40%)
 Frame = +1

Query: 265 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 444
           T  A+K     +    P T    K  + ++   A KK + G +KY    + S    +  E
Sbjct: 130 TGRATKSNAGVMDVQSPSTMSTSKNNVRNAERPASKKPVFGSKKYFDVINDSNVENKE-E 188

Query: 445 EKLRATDRQ*TAFT 486
            K R  DR  + FT
Sbjct: 189 TKDRNLDRALSKFT 202


>SPBP35G2.03c |sgo1||shugoshin Sgo1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 319

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 14/61 (22%), Positives = 31/61 (50%)
 Frame = +2

Query: 62  KTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTGECRYGLFDF 241
           K + + ++ + K ++     +D K+ D + +  +  EY  F ++L+   TG+  Y   DF
Sbjct: 108 KCSSDFLEANVKSQFTTCENKDSKE-DYQILHNKRLEYVSFNDELKSLETGQPLYCFQDF 166

Query: 242 E 244
           +
Sbjct: 167 Q 167


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,251,382
Number of Sequences: 5004
Number of extensions: 63981
Number of successful extensions: 193
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)