BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0062 (766 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 138 6e-34 SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 119 5e-28 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 116 4e-27 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 109 4e-25 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 86 5e-18 SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB comp... 30 0.42 SPAC664.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 30 0.42 SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|... 29 0.73 SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharom... 28 1.3 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 2.2 SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|c... 27 2.2 SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 26 5.1 SPAC57A7.06 |||U3 snoRNP protein Utp14 |Schizosaccharomyces pomb... 26 6.8 SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyc... 26 6.8 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 26 6.8 SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 25 9.0 SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 25 9.0 SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.0 SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 25 9.0 SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.0 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 138 bits (335), Expect = 6e-34 Identities = 67/84 (79%), Positives = 74/84 (88%) Frame = +2 Query: 2 GEQDTDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVY 181 GE+ +D IVLLDV PLT+GIET GGVMTKLI RNT IPT+KSQIFSTA DNQ+TV IQVY Sbjct: 411 GEEGSDNIVLLDVIPLTLGIETTGGVMTKLIGRNTPIPTRKSQIFSTAVDNQNTVLIQVY 470 Query: 182 EGERPMTKDNHLLGKFDLTGIPPA 253 EGER +TKDN+LLGKFDL GIPPA Sbjct: 471 EGERTLTKDNNLLGKFDLRGIPPA 494 Score = 99.5 bits (237), Expect = 5e-22 Identities = 49/89 (55%), Positives = 60/89 (67%), Gaps = 1/89 (1%) Frame = +1 Query: 244 PSCSRGIPQIEVTFEIDANGILQVSAEDK-GTGNREKIVITNDQNRLTPEDIERMIXXXX 420 P RG+PQIEVTFE+DANG+L VSA DK G G EK+VI ND+ RL+ EDIERM+ Sbjct: 492 PPAPRGVPQIEVTFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEEDIERMVKEAE 551 Query: 421 XXXXXXXXXXXRVESRNELESYAYSIKNQ 507 R+E+RN LE+YAYS+K Q Sbjct: 552 EFAEEDKILKERIEARNTLENYAYSLKGQ 580 Score = 52.8 bits (121), Expect = 5e-08 Identities = 25/59 (42%), Positives = 38/59 (64%), Gaps = 1/59 (1%) Frame = +3 Query: 513 DKEKLGAKVTDDDKAKMEEALDAAIKWLE-DNQDAESEEYKKQKKTLEDVVQPIIAKLY 686 D E+LG KV +DK + +A++ +WLE +DA EE++ Q++ L+ VV PI KLY Sbjct: 583 DDEQLGGKVDPEDKQAVLDAVEDVAEWLEIHGEDASKEEFEDQRQKLDAVVHPITQKLY 641 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 119 bits (286), Expect = 5e-28 Identities = 58/82 (70%), Positives = 67/82 (81%) Frame = +2 Query: 8 QDTDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVYEG 187 + T ++LLDV PL+MGIET GGVMT LI RNT IPTKKS+IFST SDNQ V IQV+EG Sbjct: 384 EKTQDLLLLDVAPLSMGIETAGGVMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQVFEG 443 Query: 188 ERPMTKDNHLLGKFDLTGIPPA 253 ER TKD +LLGKF+L+GIPPA Sbjct: 444 ERARTKDCNLLGKFELSGIPPA 465 Score = 101 bits (243), Expect = 9e-23 Identities = 46/89 (51%), Positives = 62/89 (69%) Frame = +1 Query: 244 PSCSRGIPQIEVTFEIDANGILQVSAEDKGTGNREKIVITNDQNRLTPEDIERMIXXXXX 423 P RG+PQIEVTF++DANGIL VSA +KGTG +KI ITND+ RL+ E+I+RM+ Sbjct: 463 PPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDRMVAEAEK 522 Query: 424 XXXXXXXXXXRVESRNELESYAYSIKNQL 510 R++++N LESYAYS++N L Sbjct: 523 YKAEDEAESGRIQAKNHLESYAYSLRNSL 551 Score = 53.6 bits (123), Expect = 3e-08 Identities = 24/60 (40%), Positives = 37/60 (61%) Frame = +3 Query: 513 DKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYKKQKKTLEDVVQPIIAKLYQA 692 D L KV DK +++A+ I+WL+ N A +E++ ++K LE V PI+AK+YQA Sbjct: 552 DDPNLKDKVDASDKETVDKAVKETIEWLDSNTTAAKDEFEAKQKELESVANPIMAKIYQA 611 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 116 bits (279), Expect = 4e-27 Identities = 55/82 (67%), Positives = 67/82 (81%) Frame = +2 Query: 8 QDTDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVYEG 187 + T ++LLDV PL++GIET GGVMT LI RNT IPTKKS++FST +DNQ V IQV+EG Sbjct: 384 EKTQDLLLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSEVFSTYADNQPGVLIQVFEG 443 Query: 188 ERPMTKDNHLLGKFDLTGIPPA 253 ER TKD +LLGKF+L+GIPPA Sbjct: 444 ERARTKDCNLLGKFELSGIPPA 465 Score = 101 bits (243), Expect = 9e-23 Identities = 46/89 (51%), Positives = 62/89 (69%) Frame = +1 Query: 244 PSCSRGIPQIEVTFEIDANGILQVSAEDKGTGNREKIVITNDQNRLTPEDIERMIXXXXX 423 P RG+PQIEVTF++DANGIL VSA +KGTG +KI ITND+ RL+ E+I+RM+ Sbjct: 463 PPAPRGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDRMVSEAEK 522 Query: 424 XXXXXXXXXXRVESRNELESYAYSIKNQL 510 R++++N LESYAYS++N L Sbjct: 523 YKAEDEAETSRIQAKNHLESYAYSLRNSL 551 Score = 54.8 bits (126), Expect = 1e-08 Identities = 29/81 (35%), Positives = 47/81 (58%), Gaps = 1/81 (1%) Frame = +3 Query: 453 TSRVK-KRARKLCLLNQKPTQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEY 629 TSR++ K + + + + D L KV DK +++A+ I+WL+ N A +EY Sbjct: 531 TSRIQAKNHLESYAYSLRNSLDDPNLKDKVDASDKEAIDKAVKETIEWLDHNTTAAKDEY 590 Query: 630 KKQKKTLEDVVQPIIAKLYQA 692 + ++K LE V PI+AK+YQA Sbjct: 591 EDKQKELEGVANPIMAKIYQA 611 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 109 bits (262), Expect = 4e-25 Identities = 49/77 (63%), Positives = 63/77 (81%) Frame = +2 Query: 23 IVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVYEGERPMT 202 +VLLDV PL++GIET+GGV T+LI RNT IPT+KSQ+FSTA+D Q V I+V++GER + Sbjct: 432 LVLLDVTPLSLGIETLGGVFTRLINRNTTIPTRKSQVFSTAADGQTAVEIRVFQGERELV 491 Query: 203 KDNHLLGKFDLTGIPPA 253 +DN L+G F LTGI PA Sbjct: 492 RDNKLIGNFQLTGIAPA 508 Score = 58.0 bits (134), Expect = 1e-09 Identities = 31/90 (34%), Positives = 48/90 (53%) Frame = +1 Query: 256 RGIPQIEVTFEIDANGILQVSAEDKGTGNREKIVITNDQNRLTPEDIERMIXXXXXXXXX 435 +G PQIEV+F++DA+GI+ VSA DK T N++ + + LT +IE M+ Sbjct: 510 KGQPQIEVSFDVDADGIINVSARDKAT-NKDSSITVAGSSGLTDSEIEAMVADAEKYRAS 568 Query: 436 XXXXXXRVESRNELESYAYSIKNQLRIRKN 525 +E+ N ES I++ L I K+ Sbjct: 569 DMARKEAIENGNRAESVCTDIESNLDIHKD 598 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 86.2 bits (204), Expect = 5e-18 Identities = 41/79 (51%), Positives = 53/79 (67%) Frame = +2 Query: 14 TDAIVLLDVNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVYEGER 193 T ++LLDV PL++G+ G V + PRNT IPT K + F+T +DNQ TVT VY+GER Sbjct: 391 TQDLLLLDVVPLSLGVAMEGNVFGVVCPRNTPIPTIKKRTFTTVADNQTTVTFPVYQGER 450 Query: 194 PMTKDNHLLGKFDLTGIPP 250 +N LG+F LTGIPP Sbjct: 451 VNCAENEPLGEFQLTGIPP 469 Score = 63.3 bits (147), Expect = 4e-11 Identities = 34/99 (34%), Positives = 55/99 (55%) Frame = +1 Query: 244 PSCSRGIPQIEVTFEIDANGILQVSAEDKGTGNREKIVITNDQNRLTPEDIERMIXXXXX 423 P RG ++E TFE+DANGIL+V+A +K TG I ITN L+ I+ MI Sbjct: 468 PPMPRGQAELEATFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQEMIENADK 527 Query: 424 XXXXXXXXXXRVESRNELESYAYSIKNQLRIRKNLVLRL 540 ++E++++LESY +I+ + N++++L Sbjct: 528 FKQQDKDFAKKLEAKSQLESYISNIETTIS-EPNVMMKL 565 >SPBC13E7.10c |brf1|SPBC30D10.20|transcription factor TFIIIB complex subunit Brf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 29.9 bits (64), Expect = 0.42 Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 5/70 (7%) Frame = +3 Query: 516 KEKLGAKVTDDDKAK-----MEEALDAAIKWLEDNQDAESEEYKKQKKTLEDVVQPIIAK 680 +E++ + DDD+ + +E L W+E N+D +EE K K ED+ + I+ + Sbjct: 389 EEEVTLEDVDDDEIEDILLDKDEILTKTQVWMELNKDYLAEEEAKNLKLQEDLKKGIVRQ 448 Query: 681 LYQARVEYRP 710 + R YRP Sbjct: 449 PRKRR-RYRP 457 >SPAC664.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 29.9 bits (64), Expect = 0.42 Identities = 13/48 (27%), Positives = 30/48 (62%) Frame = +3 Query: 537 VTDDDKAKMEEALDAAIKWLEDNQDAESEEYKKQKKTLEDVVQPIIAK 680 V D+ + E+ L+ ++ ++ N+ ESE+YK +++L V + ++A+ Sbjct: 36 VVDEYSKESEDVLENGLEHVQSNEFTESEDYKSIERSLA-VTEAVMAR 82 >SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 29.1 bits (62), Expect = 0.73 Identities = 13/36 (36%), Positives = 23/36 (63%) Frame = -1 Query: 331 PYLQQTLAKYRWHQFQM*LQFVEYHESRRDPS*VKF 224 P+ LA++++ + + LQ++EY E R+P VKF Sbjct: 30 PWYLNFLAQHKYFEDEAFLQYLEYMEYWREPEYVKF 65 >SPBC16C6.06 |pep1|vps10|sorting receptor for CPY|Schizosaccharomyces pombe|chr 2|||Manual Length = 1466 Score = 28.3 bits (60), Expect = 1.3 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = -2 Query: 363 SDYNLFPVSCSLIFSRHLQNTVGINFKCDFNLWNTTRAGGIPVKSNLPSK 214 S+Y+LF + + Q + + K DF W T A G P+ S + K Sbjct: 544 SNYSLFSFNFGKFLPKECQFSNSESNKNDFEKWYTRYANGSPICSEMGKK 593 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 27.5 bits (58), Expect = 2.2 Identities = 17/66 (25%), Positives = 31/66 (46%) Frame = +3 Query: 468 KRARKLCLLNQKPTQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYKKQKKT 647 +R R++ L K+K+ K ++ +EALD ++K ++ +E K K Sbjct: 587 QRLRRMNLYEITFLSMKQKMQLKSLREEIDNTKEALDLSVKERSIQEEKLNESLKTSKTN 646 Query: 648 LEDVVQ 665 LE+ Q Sbjct: 647 LEEQTQ 652 >SPBC19C7.10 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 27.5 bits (58), Expect = 2.2 Identities = 16/65 (24%), Positives = 28/65 (43%) Frame = +3 Query: 438 QEVEGTSRVKKRARKLCLLNQKPTQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAE 617 +E E +K ++ + Q+ EK+G + DDKAK + D + E D + Sbjct: 207 EENETKDEIKSEKKEPEIKKQEGGSSTEKVGQPSSSDDKAKGSTSKDQPSEEEEKTSDIQ 266 Query: 618 SEEYK 632 + K Sbjct: 267 DRKIK 271 >SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual Length = 539 Score = 26.2 bits (55), Expect = 5.1 Identities = 15/42 (35%), Positives = 21/42 (50%) Frame = -3 Query: 338 PVPLSSADTCKIPLASISNVTSICGIPREQEGSQLSQIYQVN 213 PVP +S T + L ++ N S+ G P E S LS + N Sbjct: 242 PVPGNSQQTIYVWLDALINYISVIGYPWLNEKSSLSAGWPAN 283 >SPAC57A7.06 |||U3 snoRNP protein Utp14 |Schizosaccharomyces pombe|chr 1|||Manual Length = 929 Score = 25.8 bits (54), Expect = 6.8 Identities = 15/56 (26%), Positives = 29/56 (51%), Gaps = 2/56 (3%) Frame = +3 Query: 492 LNQKPTQDKEKLGAKVTDDDKAKMEEALDAAIKW--LEDNQDAESEEYKKQKKTLE 653 LNQK +D+ + ++ DD +EE +K+ E N+++E +KT++ Sbjct: 235 LNQKRKKDEADAESVLSSDDNDSIEEISIKKVKYDPHETNKESEYNLIGSSEKTID 290 >SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyces pombe|chr 2|||Manual Length = 503 Score = 25.8 bits (54), Expect = 6.8 Identities = 13/44 (29%), Positives = 25/44 (56%) Frame = +3 Query: 495 NQKPTQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEE 626 +QK T ++LG K+T++ AK E+ D A + ++ + + E Sbjct: 41 DQKATNLSDRLGPKITENVDAKSEQ--DKATNTIAEDANTKQSE 82 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 25.8 bits (54), Expect = 6.8 Identities = 14/62 (22%), Positives = 29/62 (46%) Frame = +2 Query: 38 VNPLTMGIETVGGVMTKLIPRNTVIPTKKSQIFSTASDNQHTVTIQVYEGERPMTKDNHL 217 +NP+T + + V+ +I VIP +K + ST + + + + G + +N+ Sbjct: 641 LNPITNILRVIQKVIQFIISAEFVIPAQKLEAISTLVEKEFHSVVYHFIGIQRSLYENYA 700 Query: 218 LG 223 G Sbjct: 701 QG 702 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 25.4 bits (53), Expect = 9.0 Identities = 16/45 (35%), Positives = 23/45 (51%), Gaps = 1/45 (2%) Frame = +3 Query: 447 EGTSRVKKRARKLCLL-NQKPTQDKEKLGAKVTDDDKAKMEEALD 578 E S +K + + LL NQ KEKL +DD+K ++E D Sbjct: 1079 EVLSAIKSLSDSVMLLKNQIDDLAKEKLPLSSSDDEKVNIKEKTD 1123 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 25.4 bits (53), Expect = 9.0 Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 1/57 (1%) Frame = +3 Query: 507 TQDKEKLGAKVTDDDKAKMEEALDAAIKWLEDNQDAESEEYKKQKKT-LEDVVQPII 674 TQ KE G K+ + K +E K + + E EE+ KQ KT L D V+ ++ Sbjct: 506 TQLKEFEGKKLVNITKDGLELEETDEEKAAREKLEKEYEEFAKQLKTILGDKVEKVV 562 >SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 345 Score = 25.4 bits (53), Expect = 9.0 Identities = 13/39 (33%), Positives = 25/39 (64%), Gaps = 3/39 (7%) Frame = +3 Query: 549 DKAKMEEALDAAIKWLE---DNQDAESEEYKKQKKTLED 656 D + ++ L I+ LE + ++E+E+Y+KQK+ LE+ Sbjct: 150 DLLEEQQQLKLEIERLEAETERANSETEQYEKQKEALEE 188 >SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1362 Score = 25.4 bits (53), Expect = 9.0 Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 2/43 (4%) Frame = -3 Query: 296 ASISNVTSICGIPREQEGSQLSQIY--QVNDCPWSLVAHPRTL 174 AS S+V + P EQ + +I Q+ND P L +PR L Sbjct: 42 ASESHVVDVVKDPFEQYTPEEQEILYKQINDTPAKLSGYPRIL 84 >SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 25.4 bits (53), Expect = 9.0 Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 1/39 (2%) Frame = -2 Query: 459 YSFLQLLVIIGKLLSILNH-SFNIFRCQSVLVISDYNLF 346 Y ++ L+ + S LN +FN FR +L+I DY+LF Sbjct: 218 YHKVEKLLKSANIRSSLNSKTFNDFRTLFLLLIRDYSLF 256 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,783,533 Number of Sequences: 5004 Number of extensions: 52938 Number of successful extensions: 211 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 209 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 367316502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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