Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0057
         (776 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    63   4e-11
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    52   1e-07
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    48   2e-06
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    42   1e-04
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ...    38   0.002
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    37   0.004
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    36   0.006
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    29   0.74 
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    28   1.7  
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9...    27   2.3  
SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    25   9.2  
SPBC32H8.05 |||conserved fungal protein|Schizosaccharomyces pomb...    25   9.2  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 63.3 bits (147), Expect = 4e-11
 Identities = 32/87 (36%), Positives = 51/87 (58%), Gaps = 1/87 (1%)
 Frame = +2

Query: 272 EESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTG 448
           E+  I L +VD T+E DL   Y +RGYPTL  F+NG  I  YSG R+ D ++ +++K+  
Sbjct: 69  EKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL- 127

Query: 449 PPAVEVTSAEQAKELIDANTLLYLVSF 529
            P V+  S +  +  ++    L +V+F
Sbjct: 128 LPTVKPISKDTLENFVEKADDLAVVAF 154



 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 24/50 (48%), Positives = 37/50 (74%), Gaps = 1/50 (2%)
 Frame = +3

Query: 120 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAQQSW 266
           ++E+++VL   NF+ ++   T+ +LVEFYAPWCGHCK+LAP Y K  + +
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEY 402



 Score = 54.8 bits (126), Expect = 1e-08
 Identities = 20/38 (52%), Positives = 28/38 (73%)
 Frame = +3

Query: 141 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKA 254
           ++K     +IT  + ++V+FYAPWCGHCK+LAPEY  A
Sbjct: 27  VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESA 64



 Score = 41.9 bits (94), Expect = 1e-04
 Identities = 25/66 (37%), Positives = 42/66 (63%), Gaps = 6/66 (9%)
 Frame = +2

Query: 260 KLAEE---ESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDI 421
           KLAEE   +S + +AK+DAT E D++ S  + G+PT+ FF+     +P+ Y G R  +D+
Sbjct: 397 KLAEEYSDDSNVVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDL 453

Query: 422 ISWLKK 439
            +++ K
Sbjct: 454 SAFIDK 459


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 51.6 bits (118), Expect = 1e-07
 Identities = 26/83 (31%), Positives = 44/83 (53%), Gaps = 2/83 (2%)
 Frame = +2

Query: 263 LAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLK 436
           L E+ + + + K+DA    D+A+ Y + G+PTL +F  +GS P+ YS  R  D +  ++ 
Sbjct: 67  LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVS 126

Query: 437 KKTGPPAVEVTSAEQAKELIDAN 505
           +KTG    ++       EL   N
Sbjct: 127 EKTGIKKRKIVLPSNVVELDSLN 149



 Score = 48.8 bits (111), Expect = 9e-07
 Identities = 21/40 (52%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
 Frame = +3

Query: 129 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEY 245
           NV+ L   NF+ V+   +  +LVEFYA WCG+CK LAP Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180



 Score = 46.4 bits (105), Expect = 5e-06
 Identities = 24/65 (36%), Positives = 34/65 (52%)
 Frame = +3

Query: 51  IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 230
           + + +L F   AL  L     V   +++  L      T+  + +  L+EFYA WCGHCKS
Sbjct: 1   MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56

Query: 231 LAPEY 245
           LAP Y
Sbjct: 57  LAPVY 61



 Score = 39.9 bits (89), Expect = 4e-04
 Identities = 19/66 (28%), Positives = 35/66 (53%), Gaps = 3/66 (4%)
 Frame = +2

Query: 260 KLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISW 430
           K+ + E  +++ K++A    D+   + V  +PT+KFF       P  Y G R  + +I +
Sbjct: 185 KVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEY 244

Query: 431 LKKKTG 448
           + KK+G
Sbjct: 245 INKKSG 250


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 47.6 bits (108), Expect = 2e-06
 Identities = 22/62 (35%), Positives = 28/62 (45%)
 Frame = +3

Query: 66  LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 245
           L     +L+    G       N + L+  NF   +      LV FYAPWCG+CK L P Y
Sbjct: 11  LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70

Query: 246 AK 251
            K
Sbjct: 71  QK 72



 Score = 30.3 bits (65), Expect = 0.32
 Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%)
 Frame = +2

Query: 281 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 442
           P+     DA Q + +   Y V+G+PT+K       GS +   DY+G R    +  ++   
Sbjct: 82  PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141

Query: 443 TGPPAVEVTSAE 478
             P  V++ ++E
Sbjct: 142 I-PSKVKILTSE 152


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 12/43 (27%), Positives = 29/43 (67%)
 Frame = +3

Query: 138 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAQQSW 266
           V   + F++++   + ++V+F+A WCG CK++AP++ +   ++
Sbjct: 5   VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTY 47



 Score = 27.1 bits (57), Expect = 3.0
 Identities = 11/32 (34%), Positives = 17/32 (53%)
 Frame = +2

Query: 296 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 391
           KVD  Q  ++A   GV   P+   ++NG  I+
Sbjct: 54  KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85


>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 632

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 14/82 (17%), Positives = 46/82 (56%)
 Frame = +2

Query: 284 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 463
           +K+A+V+  +E+++   + ++ +PT + F+    I Y+G  +   ++S+  +     A++
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289

Query: 464 VTSAEQAKELIDANTLLYLVSF 529
           +   +  + + +++ + +LV +
Sbjct: 290 IEEGD-IESIENSHPVFFLVLY 310



 Score = 28.3 bits (60), Expect = 1.3
 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
 Frame = +2

Query: 323 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 469
           LA  YG +  P++   RNG PI Y     R+  D   I  W+   + P   E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 36.7 bits (81), Expect = 0.004
 Identities = 16/41 (39%), Positives = 23/41 (56%)
 Frame = +3

Query: 138 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAQQ 260
           V S  ++ T I+  +  +V+FYA WCG CK L P   K  +
Sbjct: 22  VESFGDYNTRISADKVTVVDFYADWCGPCKYLKPFLEKLSE 62



 Score = 36.3 bits (80), Expect = 0.005
 Identities = 19/60 (31%), Positives = 28/60 (46%)
 Frame = +2

Query: 260 KLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 439
           KL+E+        V+A +  D+A+  GV   PT+  FR G  +D   G     + S L K
Sbjct: 59  KLSEQNQKASFIAVNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
 Frame = +2

Query: 293 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 460
           AKV+  +++ +A   GV+  PT  FF NG  ID   G   QA  + +     K TG  A+
Sbjct: 56  AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115

Query: 461 EVTSAEQAK 487
             +S+   K
Sbjct: 116 ASSSSAPVK 124



 Score = 34.7 bits (76), Expect = 0.015
 Identities = 12/38 (31%), Positives = 25/38 (65%)
 Frame = +3

Query: 138 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAK 251
           + S  ++ + I  + Y+ V+ YA WCG CK+++P +++
Sbjct: 6   IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQ 43


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 29.1 bits (62), Expect = 0.74
 Identities = 10/52 (19%), Positives = 28/52 (53%)
 Frame = +2

Query: 284 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 439
           + +A ++    +   + Y ++ +PT  FF+  + ++Y G     D++S+ ++
Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382



 Score = 25.8 bits (54), Expect = 6.9
 Identities = 8/16 (50%), Positives = 11/16 (68%)
 Frame = +3

Query: 192 VEFYAPWCGHCKSLAP 239
           +++Y P CG CK L P
Sbjct: 47  IKYYLPSCGACKRLGP 62


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 9/18 (50%), Positives = 12/18 (66%)
 Frame = +3

Query: 180 EYILVEFYAPWCGHCKSL 233
           + IL+ FYAPW   CK +
Sbjct: 21  QIILLNFYAPWAAPCKQM 38


>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
           Srb9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1223

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 14/41 (34%), Positives = 19/41 (46%)
 Frame = +1

Query: 127 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 249
           K  +F       L LQP  T   N +L+   + NLW R+ P
Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660


>SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 977

 Score = 25.4 bits (53), Expect = 9.2
 Identities = 16/51 (31%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
 Frame = +2

Query: 236 TGIRQGTTKLAEEESPIKLAKV-DATQEQDLAESYGVRGYPTLKFFRNGSP 385
           +G++   T  A  +     AK  D +  Q+L ES G   YP++ FF    P
Sbjct: 642 SGLKSNNTLRALSQDLKNCAKSKDDSTTQNLTESLGSVCYPSMPFFNQYVP 692


>SPBC32H8.05 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 117

 Score = 25.4 bits (53), Expect = 9.2
 Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
 Frame = +1

Query: 541 SARAKTFLSTAQVVDDQVFAIVSDEKVIKELEAEDEDVV--LFKNFEEKRVKYEDEEITE 714
           SAR+K+     +V+ + VF  V DE+  K L A   D V  L K+   K     D  + E
Sbjct: 4   SARSKSIRRNKKVLRENVFQPVIDERT-KRLSAHLRDQVNDLTKSSSSKEEGIADNSLKE 62


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,933,643
Number of Sequences: 5004
Number of extensions: 56059
Number of successful extensions: 172
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)