BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0049 (746 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 52 1e-07 SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 39 9e-04 SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.076 SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual 29 0.93 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 28 1.6 SPAC56F8.11 |spc3||signal peptidase subunit Spc3 |Schizosaccharo... 27 2.1 SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.8 SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating |S... 26 5.0 SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase |Schiz... 26 5.0 SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|... 26 6.6 SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 6.6 SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 6.6 SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|S... 25 8.7 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 8.7 >SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual Length = 272 Score = 51.6 bits (118), Expect = 1e-07 Identities = 23/73 (31%), Positives = 44/73 (60%) Frame = +1 Query: 517 IVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVF 696 I+ E++EK S+I++N+ + I++ GN+ G +RK H+ F+ + + + P+F Sbjct: 84 IIYGFPEKEEKQSNIIYNSCIYITENGNLGGVYRKVHL-----FDTERKHFKKGSDFPIF 138 Query: 697 ATRYGKIAVNICF 735 T +GK+ V IC+ Sbjct: 139 ETSFGKLGVMICW 151 >SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 38.7 bits (86), Expect = 9e-04 Identities = 24/69 (34%), Positives = 34/69 (49%), Gaps = 5/69 (7%) Frame = +1 Query: 544 EKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESNYYMEGNTGHPVFATRY 708 E+ L+NTA+V +G +I HRK H IP F ES+ G+ + T Y Sbjct: 129 ERKDGKLYNTAMVFDPSGKLIAVHRKIHLFDIDIPGGVSFRESDSLSPGD-AMTMVDTEY 187 Query: 709 GKIAVNICF 735 GK + IC+ Sbjct: 188 GKFGLGICY 196 >SPBC336.05c |||S-adenosylmethionine- dependentmethyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 32.3 bits (70), Expect = 0.076 Identities = 21/64 (32%), Positives = 31/64 (48%) Frame = -3 Query: 483 KVVVGPSSADSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 304 ++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289 Query: 303 LTGR 292 L GR Sbjct: 290 LFGR 293 >SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual Length = 276 Score = 28.7 bits (61), Expect = 0.93 Identities = 25/89 (28%), Positives = 39/89 (43%), Gaps = 6/89 (6%) Frame = +1 Query: 487 SGTRHQVPMVIVSSILERDEKHSDILWNTAVVISDT-GNVIGKHRKNHIPRVGDFN---- 651 S T+H + V+ + K + L N+++ I G +I ++ K H+ V N Sbjct: 72 SATKHSI---FVNICVHEPSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTL 128 Query: 652 -ESNYYMEGNTGHPVFATRYGKIAVNICF 735 ESN + G P T GK+ ICF Sbjct: 129 KESNTTLRGEAILPPCKTPLGKVGSAICF 157 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 27.9 bits (59), Expect = 1.6 Identities = 9/18 (50%), Positives = 16/18 (88%) Frame = +1 Query: 511 MVIVSSILERDEKHSDIL 564 +++VS++LE DEKH D++ Sbjct: 998 LIVVSNLLEMDEKHVDVV 1015 >SPAC56F8.11 |spc3||signal peptidase subunit Spc3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 185 Score = 27.5 bits (58), Expect = 2.1 Identities = 15/61 (24%), Positives = 30/61 (49%) Frame = +1 Query: 493 TRHQVPMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYME 672 T+H V ++ S E+ EK+ ++W+ + + + K ++I + FNE + E Sbjct: 87 TKHVVVYLVASYSTEKHEKNQVVVWDKILSSPEESKMFMKDTLSNI-QAHPFNEYSNQFE 145 Query: 673 G 675 G Sbjct: 146 G 146 >SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosaccharomyces pombe|chr 2|||Manual Length = 2352 Score = 26.6 bits (56), Expect = 3.8 Identities = 11/25 (44%), Positives = 18/25 (72%) Frame = -1 Query: 743 CRPKQMFTAILPYLVANTGWPVLPS 669 C+P +FTA+ P+L A T + ++PS Sbjct: 1528 CKP--VFTAVPPFLFAGTDFALIPS 1550 >SPBC660.16 |||phosphogluconate dehydrogenase, decarboxylating |Schizosaccharomyces pombe|chr 2|||Manual Length = 492 Score = 26.2 bits (55), Expect = 5.0 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Frame = -1 Query: 698 ANTGWPVLPSM**LDSLKSPTLGMWFLR-CFPITFPVSLITTAVFQSMSECFSSLSN 531 A+ G P++ + LD+ G W + + PVSLIT AVF + C SSL + Sbjct: 247 ADDGKPLVEKI--LDAAGQKGTGKWTAQNALEMGTPVSLITEAVF---ARCLSSLKS 298 >SPBC27.08c |sua1|SPBC28F2.01c|sulfate adenylyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 26.2 bits (55), Expect = 5.0 Identities = 13/29 (44%), Positives = 14/29 (48%) Frame = -3 Query: 723 HRDLAVSGRKYRMAGVTFHVVVGFVKIAD 637 HR+L V K A V H VVG K D Sbjct: 202 HRELTVRAAKQHGARVLIHPVVGMTKPGD 230 >SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 972 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -2 Query: 556 PNASRPFLISRTRSPLVLDGEFPKEGRGRP 467 P ++P ++ T + +D PK GRGRP Sbjct: 316 PPLNKPKILFGTSTENKIDENRPKRGRGRP 345 >SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosaccharomyces pombe|chr 2|||Manual Length = 502 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 75 NNLTGRDLEEFNRIHFGRRNNLEI 146 NN+ R +EE N I G+R LE+ Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31 >SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 499 Score = 25.8 bits (54), Expect = 6.6 Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Frame = +3 Query: 45 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 155 E H +E +I + N+T DL F + FG+ N ++ K Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406 >SPAC17A5.04c |mde10|mug139|spore wall assembly peptidase Mde10|Schizosaccharomyces pombe|chr 1|||Manual Length = 512 Score = 25.4 bits (53), Expect = 8.7 Identities = 8/25 (32%), Positives = 14/25 (56%) Frame = -2 Query: 727 CSPRSCRIWSQIQDGRCYLPCSSWI 653 C SC++ Q +DG C++ +I Sbjct: 438 CHSDSCKVSCQNEDGTCFISAKDYI 462 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.4 bits (53), Expect = 8.7 Identities = 24/88 (27%), Positives = 37/88 (42%), Gaps = 1/88 (1%) Frame = +2 Query: 434 PWCEFAESAEDGPTTTFLRELAIKYQW*SCPRY*KGTRSIRTYFGTLRL*LVTPET*SGN 613 P C + + G T L LA ++W PR G+R + F L L + N Sbjct: 523 PSCHWTSVSLGGAVATLLMLLATIFEWIHVPRKFPGSRPLLKRFLILILFFIL------N 576 Query: 614 IARTTFRES-AILTNPTTTWKVTPAILY 694 +A T F + TT ++T AI++ Sbjct: 577 VAPTVFVFGFSTEEQQRTTGRLTVAIVH 604 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,192,339 Number of Sequences: 5004 Number of extensions: 67624 Number of successful extensions: 242 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 222 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 241 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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