BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0029 (740 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 37 0.003 SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 31 0.17 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 29 0.92 SPAC26F1.02 |||pinin homologue|Schizosaccharomyces pombe|chr 1||... 28 1.6 SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyce... 27 2.1 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 27 2.8 SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc... 27 2.8 SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 27 3.7 SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 3.7 SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharom... 27 3.7 SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.9 SPAC17G6.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 4.9 SPCC622.05 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 26 6.5 SPAC3A11.06 |mvp1||sorting nexin Mvp1|Schizosaccharomyces pombe|... 26 6.5 SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 6.5 SPAC3H1.02c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|... 25 8.6 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 8.6 >SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 36.7 bits (81), Expect = 0.003 Identities = 33/112 (29%), Positives = 59/112 (52%), Gaps = 4/112 (3%) Frame = +2 Query: 257 NKELETALRQHQNVIEDQAQEIEYLTKQTVALRE-VNDS-RLRIY-EQLELSIQDLERAN 427 N E + L++ + ++++ +E+ L K LRE DS +LR+ EQLEL QDL++A Sbjct: 497 NNEFDRQLKEQEEDLQNKEEELTELRK---LLREQTQDSQKLRLLVEQLELERQDLKQAG 553 Query: 428 HRLAVDHAAD-KKHIKSLCSNIDTVEAKAKNYKRLWTNLTFN*RSTGEELNE 580 + ++D + IKSL S++ +A+ +++ L +LNE Sbjct: 554 ENHYSNLSSDYETQIKSLESSLTNSQAECVSFQEKINELNSQIDELKLKLNE 605 >SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1727 Score = 31.1 bits (67), Expect = 0.17 Identities = 29/109 (26%), Positives = 53/109 (48%) Frame = +2 Query: 254 SNKELETALRQHQNVIEDQAQEIEYLTKQTVALREVNDSRLRIYEQLELSIQDLERANHR 433 SNK L L +++ VI + ++IE L K +A +N EQLE +Q + A + Sbjct: 1238 SNKSLRENLERNEEVITELREKIETL-KTDLANFRLNK------EQLESQLQTEKAAVKK 1290 Query: 434 LAVDHAADKKHIKSLCSNIDTVEAKAKNYKRLWTNLTFN*RSTGEELNE 580 L + K+H + + ++++ + + + RL L + EELN+ Sbjct: 1291 LENSNEEYKRHNQEILLSLNSSTSTSSDASRLKNELVSK-ENLIEELNQ 1338 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 28.7 bits (61), Expect = 0.92 Identities = 35/170 (20%), Positives = 75/170 (44%), Gaps = 10/170 (5%) Frame = +2 Query: 44 EPLRIMINLCDKNTSMYRIVP*TVNNVRSNGSGE--EEIRWKRRVGARRYHLTSLYARRF 217 + L I + +N+S + + V+ N E +R ++ + +Y LT L R Sbjct: 1168 DSLSQQITVLQQNSSENLNISANLEAVQDNDLRELVSYLRHEKEIMDNKYELTILDNRGL 1227 Query: 218 ATGGRTRENPTRSNKELETALRQHQNVIEDQ--------AQEIEYLTKQTVALREVNDSR 373 ++ ++ T + +LE Q V DQ +QE++ L + LR+ ND++ Sbjct: 1228 NQQVKSLQS-TVDSLQLELNRLQSLPVSNDQTDTPIISGSQEVQLLYESNSVLRKDNDAK 1286 Query: 374 LRIYEQLELSIQDLERANHRLAVDHAADKKHIKSLCSNIDTVEAKAKNYK 523 L ++LE ++ L + + L + K I + ++++ ++ +K Sbjct: 1287 LGKIQELEKEVEKLNASLNPLQTEINELKAEIGAKTASLNLMKEYNSRWK 1336 >SPAC26F1.02 |||pinin homologue|Schizosaccharomyces pombe|chr 1|||Manual Length = 197 Score = 27.9 bits (59), Expect = 1.6 Identities = 18/85 (21%), Positives = 40/85 (47%), Gaps = 2/85 (2%) Frame = +2 Query: 200 LYARRFATGGRTRENPTRSNKELETALRQH--QNVIEDQAQEIEYLTKQTVALREVNDSR 373 ++ T G+ ++ R K R + + + + QE++ L KQ E+ +SR Sbjct: 76 MFGALLGTLGKFQQESEREQKSARKVKRAELEEKLAKRREQELQELEKQEKIEAEILESR 135 Query: 374 LRIYEQLELSIQDLERANHRLAVDH 448 L+ ++ L +L+R + + +D+ Sbjct: 136 LQEQRKVALDELELDRNDLKKVLDN 160 >SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 855 Score = 27.5 bits (58), Expect = 2.1 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = +2 Query: 299 IEDQAQEIEYLTKQTVALREVNDSRLRIYEQLELSIQ 409 I+ + Q I+ + T L NDSR+RIY + S++ Sbjct: 447 IQTRTQMIDNIAGDTEMLVTTNDSRIRIYNLRDKSLE 483 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 27.1 bits (57), Expect = 2.8 Identities = 24/88 (27%), Positives = 38/88 (43%), Gaps = 2/88 (2%) Frame = +2 Query: 161 KRRVGARRYHLTSLYARRFATGGRTRENPTR-SNKELE-TALRQHQNVIEDQAQEIEYLT 334 + ++ A R AR A + +E + S KE E +L + E Q +E+E T Sbjct: 5 REKINAARAETDEAVARAEAAEAKLKEVELQLSLKEQEYESLSRKSEAAESQLEELEEET 64 Query: 335 KQTVALREVNDSRLRIYEQLELSIQDLE 418 KQ + D + EQL ++ LE Sbjct: 65 KQLRLKADNEDIQKTEAEQLSRKVELLE 92 >SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosaccharomyces pombe|chr 2|||Manual Length = 300 Score = 27.1 bits (57), Expect = 2.8 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = +2 Query: 143 EEEIRWKRRVGARRYHLTSLYARRFATGGRTRENPTRSNKELETA 277 E+ +RWKR RR++ L+ + R + N LETA Sbjct: 246 EKRLRWKRENQLRRHNFVGLFVELSKLLVKDRIDKNTWNSTLETA 290 >SPCC1827.04 |||ankyrin repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 3|||Manual Length = 600 Score = 26.6 bits (56), Expect = 3.7 Identities = 20/59 (33%), Positives = 27/59 (45%) Frame = +2 Query: 215 FATGGRTRENPTRSNKELETALRQHQNVIEDQAQEIEYLTKQTVALREVNDSRLRIYEQ 391 F T T R KEL T H + I +AQE E +Q +E+ SRL+ E+ Sbjct: 328 FTTKRATHSELLRCYKELVTPKISHVDSISIKAQE-EERKRQAEIEKEIRQSRLQEEER 385 >SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces pombe|chr 3|||Manual Length = 1526 Score = 26.6 bits (56), Expect = 3.7 Identities = 17/57 (29%), Positives = 30/57 (52%), Gaps = 3/57 (5%) Frame = +2 Query: 257 NKELETALRQHQNVIEDQAQEIEYLTKQTVALREVNDSRL---RIYEQLELSIQDLE 418 NKELE +++ N I+ +E+E ++ L + + + I+E L L + DLE Sbjct: 1112 NKELEAKVKECLNNIKSLTKELENKEEKCQNLSDASLKYIELQEIHENLLLKVSDLE 1168 >SPCP1E11.08 |||ribosome biogenesis protein Nsa2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 260 Score = 26.6 bits (56), Expect = 3.7 Identities = 28/147 (19%), Positives = 61/147 (41%), Gaps = 4/147 (2%) Frame = +2 Query: 119 NVRSNGSGEEEIRWKRRVGARRYHLTSLYARRF-ATGGRTRENPTRSNK-ELETALRQHQ 292 ++R +G + KR+ AR H SLYA++ + + R K +++ ++QH+ Sbjct: 10 SIRKHGRRFDHEERKRKKAAREAHDASLYAQKTRGIKAKLYQEKRRKEKIQMKKTIKQHE 69 Query: 293 --NVIEDQAQEIEYLTKQTVALREVNDSRLRIYEQLELSIQDLERANHRLAVDHAADKKH 466 N + + T L +S+ ++ + + A + + + Sbjct: 70 ERNATQRGSDAQTQGAVPTYLLDREQESQAKMLSSAVKQKRKEKAAKYSVPLPQVRGVAE 129 Query: 467 IKSLCSNIDTVEAKAKNYKRLWTNLTF 547 + + I T ++K ++KR+ T TF Sbjct: 130 -EEMFKVIRTGKSKKNSWKRMITKATF 155 >SPAC323.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 575 Score = 26.2 bits (55), Expect = 4.9 Identities = 10/23 (43%), Positives = 17/23 (73%) Frame = +3 Query: 288 IKMLLKIKHKKSNILQSKRLLCE 356 ++ L K +++KSN+L + R LCE Sbjct: 266 LESLAKSQYRKSNVLAADRSLCE 288 >SPAC17G6.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 26.2 bits (55), Expect = 4.9 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +1 Query: 505 QSEELQKTVDELNVQLEIYRRRAER 579 Q LQ ++ELN Q+E+ +R+ E+ Sbjct: 19 QDHSLQMALEELNQQIEVIQRQEEQ 43 >SPCC622.05 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual Length = 115 Score = 25.8 bits (54), Expect = 6.5 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -3 Query: 144 SPDPLLLTLFTVYGTMRYIDVF 79 SP P L LF+V G + +DVF Sbjct: 48 SPSPFLRWLFSVLGVLMSMDVF 69 >SPAC3A11.06 |mvp1||sorting nexin Mvp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 664 Score = 25.8 bits (54), Expect = 6.5 Identities = 17/62 (27%), Positives = 32/62 (51%), Gaps = 1/62 (1%) Frame = +2 Query: 236 RENPTRSNKELETALRQHQNVIEDQAQEI-EYLTKQTVALREVNDSRLRIYEQLELSIQD 412 REN R ++E+E L Q+ + D ++ + E L K ++ +++L I+ E I Sbjct: 400 RENQKRIDQEIEQFLVVPQSQVPDASETVKERLLKLNMSTTTAINNQLNIFRIFEKMIFT 459 Query: 413 LE 418 L+ Sbjct: 460 LQ 461 >SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 339 Score = 25.8 bits (54), Expect = 6.5 Identities = 16/37 (43%), Positives = 19/37 (51%) Frame = +3 Query: 420 ELTTD*LSIMQPTRNTLSHYAQTLTLWRPKRRTTKDC 530 ELT D + T N LSHY T L RRT ++C Sbjct: 137 ELTKDGYELQIQT-NYLSHYLFTELLLPTLRRTAEEC 172 >SPAC3H1.02c |||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 25.4 bits (53), Expect = 8.6 Identities = 18/62 (29%), Positives = 30/62 (48%) Frame = +3 Query: 135 DLVKKKFGGNEELEQDDITSRRCMLDDLQLAAELGKTLLEATRNSRQPFASIKMLLKIKH 314 DLVK G ++ D+ S +C+ + +EL + +++NS SIK L K + Sbjct: 896 DLVKSLISGEVQVSSFDLESAKCV--SYSIVSELENNAIYSSKNS-FTLLSIKGLNKDED 952 Query: 315 KK 320 K Sbjct: 953 HK 954 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 25.4 bits (53), Expect = 8.6 Identities = 15/59 (25%), Positives = 27/59 (45%) Frame = +2 Query: 260 KELETALRQHQNVIEDQAQEIEYLTKQTVALREVNDSRLRIYEQLELSIQDLERANHRL 436 KE+ AL + + + +EIE L + L+ + + + + +I DLE N L Sbjct: 936 KEVSQALAEANEKLNARDEEIERLKVDIIGLQNASLNMQSLKDSDNRTISDLESKNKEL 994 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,599,773 Number of Sequences: 5004 Number of extensions: 47658 Number of successful extensions: 175 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 169 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 175 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 351258950 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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