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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= e40h0026
         (629 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr 1|...    62   8e-11
SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr 3|||Ma...    34   0.019
SPAC17H9.11 |||cofilin/tropomyosin family protein|Schizosaccharo...    29   0.42 
SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ...    29   0.73 
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom...    29   0.73 
SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    28   1.3  
SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    27   2.2  
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc...    27   3.0  
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    26   5.2  
SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr 1||...    25   6.8  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    25   6.8  
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual     25   9.0  
SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|...    25   9.0  

>SPAC20G4.06c |adf1|cof1|cofilin|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 137

 Score = 61.7 bits (143), Expect = 8e-11
 Identities = 27/57 (47%), Positives = 38/57 (66%)
 Frame = +1

Query: 295 KQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVEEKL 465
           + K+  +SW PD A +K KM+YSSS D L+++  G+   IQATD SE + E V EK+
Sbjct: 78  RNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSEVAYETVLEKV 134



 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 26/70 (37%), Positives = 39/70 (55%)
 Frame = +2

Query: 47  SGVTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDVETVGERNAEYEQFLEDLQKGGTG 226
           SGV VS  C   ++E+K  K  RYVVF + D K   V      + +++ FL DL +    
Sbjct: 4   SGVKVSPECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKSTDKDFDTFLGDLPE---K 60

Query: 227 ECRYGLFDFD 256
           +CRY ++DF+
Sbjct: 61  DCRYAIYDFE 70


>SPCC126.06 |twf1||twinfilin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 328

 Score = 33.9 bits (74), Expect = 0.019
 Identities = 15/27 (55%), Positives = 21/27 (77%)
 Frame = +1

Query: 289 SKKQKLFLMSWCPDTAKVKKKMLYSSS 369
           SKK  L L+S+ P+ A V++KMLY+SS
Sbjct: 76  SKKNLLQLISYVPENANVRRKMLYASS 102



 Score = 33.1 bits (72), Expect = 0.034
 Identities = 22/82 (26%), Positives = 41/82 (50%), Gaps = 5/82 (6%)
 Frame = +1

Query: 301 KLFLMSWCPDTAKVKKKMLYSSS----FDALKKSL-VGVQKYIQATDLSEASQEAVEEKL 465
           K+  +  CP  A VK +M+YSSS     D++K  L + +   I++ D ++ +++ +    
Sbjct: 244 KILFIYICPMQATVKHRMVYSSSKLGLLDSIKAELGIVIDGKIESNDAADITEKEILHAA 303

Query: 466 RATDRQ*TAFTHELGRNRTRSP 531
             +  Q    T + G +R R P
Sbjct: 304 GISSPQAETSTTKTGFSRPRPP 325


>SPAC17H9.11 |||cofilin/tropomyosin family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 141

 Score = 29.5 bits (63), Expect = 0.42
 Identities = 17/63 (26%), Positives = 30/63 (47%)
 Frame = +1

Query: 277 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 456
           T++      LF++ W P        M+Y+S+     + +  V K  +A D  + + EAV+
Sbjct: 79  TTDGRLSTPLFMIYWRPSATPNDLSMIYASA-KVWFQDVSQVHKVFEARDSEDITSEAVD 137

Query: 457 EKL 465
           E L
Sbjct: 138 EFL 140


>SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 335

 Score = 28.7 bits (61), Expect = 0.73
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = -3

Query: 534 CRRAGSVSSELVCKCCLLAIG 472
           C R G +SS  +CK C+L  G
Sbjct: 287 CERCGFISSNRICKACMLLEG 307


>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 447

 Score = 28.7 bits (61), Expect = 0.73
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 470 PPIANKQHLHTSSDETEPALRHSCPDDTRPRHH 568
           PP++ K   + S    EP +RHS     RP+ H
Sbjct: 37  PPLSRKNPSNVSFWSNEPIIRHSSVKTDRPQFH 69


>SPBP4H10.18c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 314

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 21/80 (26%), Positives = 32/80 (40%)
 Frame = +1

Query: 277 TSEASKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQATDLSEASQEAVE 456
           T  A+K     +    P T    K  + ++   A KK + G +KY    + S    +  E
Sbjct: 130 TGRATKSNAGVMDVQSPSTMSTSKNNVRNAERPASKKPVFGSKKYFDVINDSNVENKE-E 188

Query: 457 EKLRATDRQ*TAFTHELGRN 516
            K R  DR  + FT    +N
Sbjct: 189 TKDRNLDRALSKFTQSREKN 208


>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 27.1 bits (57), Expect = 2.2
 Identities = 10/26 (38%), Positives = 19/26 (73%)
 Frame = +1

Query: 367 SFDALKKSLVGVQKYIQATDLSEASQ 444
           S +AL++ L G + Y+Q+TD++  S+
Sbjct: 188 SLEALQEELEGFEDYVQSTDIAAMSK 213


>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
            Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1958

 Score = 26.6 bits (56), Expect = 3.0
 Identities = 16/46 (34%), Positives = 23/46 (50%), Gaps = 6/46 (13%)
 Frame = -1

Query: 332  VSGHHDIRKSFCFLLASDVPW----HWCVYQSQTGHIC-IPR-YRP 213
            + G  ++    C L AS   W    HWC + ++TG +C  PR Y P
Sbjct: 1761 IPGVIELSNHLC-LTASSTEWSLIKHWCNFFTETGPLCDFPRAYYP 1805


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 25.8 bits (54), Expect = 5.2
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +3

Query: 462 APRHRSPINSIYTRARTKPNPLSDTP 539
           APR R P  ++ T A  +P P+S  P
Sbjct: 24  APRKREPARTVSTPAFMEPAPVSKKP 49


>SPAC6C3.09 |||RNase P subunit |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 335

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
 Frame = -1

Query: 518 RFRPSSCVNAVYWRSVARSFSSTAS*DASE--RSVAWMYF*TPTRDFFR 378
           R++PS  V  V + S+   FSS+   +  E  R+++W +    + + FR
Sbjct: 254 RYKPSCAVTDVLYLSIKGLFSSSTCLELYELIRNISWNFIGVESFNSFR 302


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 25.4 bits (53), Expect = 6.8
 Identities = 13/31 (41%), Positives = 18/31 (58%), Gaps = 1/31 (3%)
 Frame = -1

Query: 305 SFCF-LLASDVPWHWCVYQSQTGHICIPRYR 216
           + CF LLA  + +    YQ Q GHI  PR++
Sbjct: 28  TICFALLAVCIAYDQISYQMQKGHIPGPRFK 58


>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 366

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 10/22 (45%), Positives = 11/22 (50%)
 Frame = +2

Query: 56  TVSDACKTTYEEIKKDKKHRYV 121
           TV D C   +  IK   KH YV
Sbjct: 316 TVEDFCNNIHSSIKSQFKHAYV 337


>SPBP26C9.03c |||iron ion transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 584

 Score = 25.0 bits (52), Expect = 9.0
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +2

Query: 53  VTVSDACKTTYEEIKKDKKHRYVVFYIRDEKQIDV 157
           VT  D  KT  +  +K  KH+ V  YIR  K +D+
Sbjct: 113 VTNDDGTKTKVDSDEKKHKHKNVRDYIR-FKHVDI 146


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,325,394
Number of Sequences: 5004
Number of extensions: 43953
Number of successful extensions: 144
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 279695522
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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