Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0021
(611 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q6GLJ2 Cluster: LOC443718 protein; n=4; Tetrapoda|Rep: ... 74 3e-12
UniRef50_Q3B726 Cluster: DNA-directed RNA polymerase I subunit R... 72 1e-11
UniRef50_UPI00005A2C8E Cluster: PREDICTED: similar to TWIST neig... 71 3e-11
UniRef50_Q4RKE7 Cluster: Chromosome 21 SCAF15029, whole genome s... 64 2e-09
UniRef50_Q6PHG8 Cluster: DNA-directed RNA polymerase I subunit R... 62 8e-09
UniRef50_A7RZD8 Cluster: Predicted protein; n=1; Nematostella ve... 56 7e-07
UniRef50_Q9VM72 Cluster: CG13773-PA; n=2; Sophophora|Rep: CG1377... 50 3e-05
UniRef50_Q16XZ3 Cluster: Putative uncharacterized protein; n=1; ... 50 3e-05
UniRef50_Q7PV20 Cluster: ENSANGP00000016750; n=1; Anopheles gamb... 49 8e-05
UniRef50_Q6C2P5 Cluster: Yarrowia lipolytica chromosome F of str... 39 0.081
UniRef50_O43036 Cluster: DNA-directed RNA polymerase I subunit R... 37 0.33
UniRef50_Q6BX66 Cluster: Similar to CA2272|CaRPA43 Candida albic... 36 0.57
UniRef50_A6SBP0 Cluster: Putative uncharacterized protein; n=2; ... 36 1.0
UniRef50_A3LV72 Cluster: DNA-dependent RNA polymerase I subunit ... 36 1.0
UniRef50_Q00W27 Cluster: DNA polymerase epsilon, subunit C; n=3;... 34 2.3
UniRef50_A0CSJ2 Cluster: Chromosome undetermined scaffold_26, wh... 33 4.0
UniRef50_Q55FA4 Cluster: RNA polymerase I subunit; n=1; Dictyost... 33 7.1
>UniRef50_Q6GLJ2 Cluster: LOC443718 protein; n=4; Tetrapoda|Rep:
LOC443718 protein - Xenopus laevis (African clawed frog)
Length = 477
Score = 73.7 bits (173), Expect = 3e-12
Identities = 34/85 (40%), Positives = 48/85 (56%)
Frame = +3
Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
Y N ++ +G I +D IH V+AD+ IF+P G L G+VNK TH+ LVH FN
Sbjct: 94 YDNIKLVGELGDIYDDLGHIHINVEADFVIFKPDCGQTLVGIVNKVAPTHIGCLVHGCFN 153
Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
IP+P + P + W+ VK E+
Sbjct: 154 ASIPKPPKMPIENWLRIGVKIDDEI 178
Score = 34.7 bits (76), Expect = 1.8
Identities = 20/57 (35%), Positives = 33/57 (57%)
Frame = +1
Query: 85 ELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKELNGVLVS 255
E +L + + SCLVV+ ++LAL P L + I L+ ++ K+ + LNGV V+
Sbjct: 37 EACELVSCRYSCLVVETHRRHLALSPKFLRKKRSGIQEQLNTELLKYSERLNGVPVA 93
>UniRef50_Q3B726 Cluster: DNA-directed RNA polymerase I subunit
RPA43; n=16; Amniota|Rep: DNA-directed RNA polymerase I
subunit RPA43 - Homo sapiens (Human)
Length = 338
Score = 71.7 bits (168), Expect = 1e-11
Identities = 32/85 (37%), Positives = 48/85 (56%)
Frame = +3
Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
Y N ++ +G I +D IH ++AD+ IF P G KL G+VNK S+H+ LVH FN
Sbjct: 93 YDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGQKLMGIVNKVSSSHIGCLVHGCFN 152
Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
IP+P + ++W + G E+
Sbjct: 153 ASIPKPEQLSAEQWQTMEINMGDEL 177
>UniRef50_UPI00005A2C8E Cluster: PREDICTED: similar to TWIST
neighbor; n=1; Canis lupus familiaris|Rep: PREDICTED:
similar to TWIST neighbor - Canis familiaris
Length = 423
Score = 70.5 bits (165), Expect = 3e-11
Identities = 32/85 (37%), Positives = 48/85 (56%)
Frame = +3
Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
Y N ++ +G I +D IH ++AD+ IF P G KL G+VNK S+H+ LVH FN
Sbjct: 171 YDNIKVVGELGDIYDDQGHIHLNIEADFVIFCPEPGKKLMGIVNKVSSSHIGCLVHGCFN 230
Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
IP+P + ++W + G E+
Sbjct: 231 ASIPKPEQMSTEQWQILEINVGDEL 255
>UniRef50_Q4RKE7 Cluster: Chromosome 21 SCAF15029, whole genome
shotgun sequence; n=3; Tetraodontidae|Rep: Chromosome 21
SCAF15029, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 352
Score = 64.5 bits (150), Expect = 2e-09
Identities = 32/74 (43%), Positives = 40/74 (54%)
Frame = +3
Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
Y N IL G I +D +H V AD+ +FQP G KL G VNK +H+ LVH FN
Sbjct: 109 YDNIRILGQYGDIVDDGGYVHMDVIADFIVFQPQKGQKLLGKVNKLGVSHVGCLVHGCFN 168
Query: 435 VVIPQPTEEPGDKW 476
IP+P P + W
Sbjct: 169 ASIPKPNLVPMETW 182
>UniRef50_Q6PHG8 Cluster: DNA-directed RNA polymerase I subunit
RPA43; n=3; Danio rerio|Rep: DNA-directed RNA polymerase
I subunit RPA43 - Danio rerio (Zebrafish) (Brachydanio
rerio)
Length = 391
Score = 62.5 bits (145), Expect = 8e-09
Identities = 28/85 (32%), Positives = 47/85 (55%)
Frame = +3
Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
Y N ++ G I +D IHF ++A + IF+P G++L G++NK ++H+ LVH FN
Sbjct: 101 YDNIKVVGQHGNIYDDQGFIHFNIEASFVIFRPKNGSRLMGVINKMGASHVGCLVHGCFN 160
Query: 435 VVIPQPTEEPGDKWIGFSVKEGQEV 509
+ +P D+W + GQ +
Sbjct: 161 ASVMKPNALTSDQWRDSGLCVGQSL 185
>UniRef50_A7RZD8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 159
Score = 56.0 bits (129), Expect = 7e-07
Identities = 28/71 (39%), Positives = 37/71 (52%)
Frame = +3
Query: 255 YKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFN 434
Y +LQ G I +++ +HF Q DY F+P IG KL G+VNK H+ LVH FN
Sbjct: 52 YDKVTLLQRRGNIIDESPFLHFDAQVDYITFKPEIGNKLIGVVNKFGYDHIGCLVHGCFN 111
Query: 435 VVIPQPTEEPG 467
I + G
Sbjct: 112 ASIAKSRTSNG 122
>UniRef50_Q9VM72 Cluster: CG13773-PA; n=2; Sophophora|Rep:
CG13773-PA - Drosophila melanogaster (Fruit fly)
Length = 253
Score = 50.4 bits (115), Expect = 3e-05
Identities = 22/62 (35%), Positives = 37/62 (59%)
Frame = +3
Query: 258 KNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNV 437
KN +L +R D+ +H + AD+++F+P GA L+G+V H+S +++RVFN
Sbjct: 74 KNIKVLGQTAGLRADDPTMHLVINADFYVFRPKAGAILSGVVRHISRHHVSAVIYRVFNT 133
Query: 438 VI 443
I
Sbjct: 134 SI 135
Score = 48.8 bits (111), Expect = 1e-04
Identities = 25/71 (35%), Positives = 42/71 (59%), Gaps = 1/71 (1%)
Frame = +1
Query: 52 IMSKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLL-DYKVGKFD 228
I+ K IKF ++EL+ A+ SC+ +LA+ P+ + N K ++ LL KVG +D
Sbjct: 4 ILQKYIKFSVKELENYASSPESCVRCITTDMHLAMGPYGMANFKHALHELLIRTKVGFYD 63
Query: 229 KELNGVLVSIK 261
L+G+++ IK
Sbjct: 64 SGLDGIVLGIK 74
>UniRef50_Q16XZ3 Cluster: Putative uncharacterized protein; n=1;
Aedes aegypti|Rep: Putative uncharacterized protein -
Aedes aegypti (Yellowfever mosquito)
Length = 571
Score = 50.4 bits (115), Expect = 3e-05
Identities = 22/70 (31%), Positives = 41/70 (58%)
Frame = +3
Query: 234 VERCLSKYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSV 413
V+ + +KN IL + IR ++ IH +V +++++F+P GA + G + +LS
Sbjct: 66 VDGIILAFKNTKILSPLSAIRPNSVRIHVKVSSNFYVFRPISGATIEGTIKYVSKNYLSA 125
Query: 414 LVHRVFNVVI 443
+++ VFNV I
Sbjct: 126 VIYGVFNVTI 135
Score = 47.6 bits (108), Expect = 2e-04
Identities = 21/68 (30%), Positives = 41/68 (60%)
Frame = +1
Query: 58 SKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKEL 237
+K K+ + EL+ D NSC+ + T +++ P LG+I E + L K+GK+D+ +
Sbjct: 7 TKYAKYTVEELESYVADPNSCVQKLEWTNLVSVLPKYLGDICEGVKACLTQKIGKYDRTV 66
Query: 238 NGVLVSIK 261
+G++++ K
Sbjct: 67 DGIILAFK 74
>UniRef50_Q7PV20 Cluster: ENSANGP00000016750; n=1; Anopheles gambiae
str. PEST|Rep: ENSANGP00000016750 - Anopheles gambiae
str. PEST
Length = 246
Score = 49.2 bits (112), Expect = 8e-05
Identities = 20/54 (37%), Positives = 33/54 (61%)
Frame = +3
Query: 282 VGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
+ +R D+ +H + DY++FQP IG+ L G VN +S +++RVFNV +
Sbjct: 82 LSALRLDSPYLHVRTTIDYYVFQPRIGSTLRGTVNYVSKNFVSAVIYRVFNVTV 135
Score = 39.1 bits (87), Expect = 0.081
Identities = 17/66 (25%), Positives = 38/66 (57%)
Frame = +1
Query: 55 MSKIIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKVGKFDKE 234
++K KF EL++ D SC+ ++ + L+L+P +I + + + K+G++ +
Sbjct: 6 IAKYTKFSAEELQKSTADPLSCVAAVQLNEILSLRPRDCEHIVKGVKRCVTDKIGRYHAK 65
Query: 235 LNGVLV 252
LNG+++
Sbjct: 66 LNGIVL 71
>UniRef50_Q6C2P5 Cluster: Yarrowia lipolytica chromosome F of strain
CLIB122 of Yarrowia lipolytica; n=1; Yarrowia
lipolytica|Rep: Yarrowia lipolytica chromosome F of
strain CLIB122 of Yarrowia lipolytica - Yarrowia
lipolytica (Candida lipolytica)
Length = 288
Score = 39.1 bits (87), Expect = 0.081
Identities = 16/54 (29%), Positives = 29/54 (53%)
Frame = +3
Query: 282 VGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
+G N++ + D +++P +G +L G VN + +H+ +LVH FN I
Sbjct: 98 MGKFVNESPFTFMWISTDLLVWKPQVGDRLEGYVNMQTPSHIGLLVHDTFNCSI 151
>UniRef50_O43036 Cluster: DNA-directed RNA polymerase I subunit
RPA43; n=1; Schizosaccharomyces pombe|Rep: DNA-directed
RNA polymerase I subunit RPA43 - Schizosaccharomyces
pombe (Fission yeast)
Length = 173
Score = 37.1 bits (82), Expect = 0.33
Identities = 23/83 (27%), Positives = 38/83 (45%)
Frame = +3
Query: 231 RVERCLSKYKNPCILQNVGTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLS 410
R+ + Y N L+ + D+ V+ D +F P G L G +N +H+
Sbjct: 43 RINGIVLAYDNIRFLEKSAKVMYDSPFSFIWVRVDVLVFSPKKGDCLEGKINLVSPSHIG 102
Query: 411 VLVHRVFNVVIPQPTEEPGDKWI 479
+L+ +FN IP+ + P D WI
Sbjct: 103 LLILGIFNASIPRKS-IPKD-WI 123
>UniRef50_Q6BX66 Cluster: Similar to CA2272|CaRPA43 Candida albicans
CaRPA43 DNA-directed RNA polymerase I; n=3;
Saccharomycetales|Rep: Similar to CA2272|CaRPA43 Candida
albicans CaRPA43 DNA-directed RNA polymerase I -
Debaryomyces hansenii (Yeast) (Torulaspora hansenii)
Length = 311
Score = 36.3 bits (80), Expect = 0.57
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +3
Query: 324 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
V D+ I++P +G L G + + ++H+ +LVH FN I
Sbjct: 122 VSVDFLIWRPQVGDTLEGYIYMQTASHIGLLVHDTFNASI 161
>UniRef50_A6SBP0 Cluster: Putative uncharacterized protein; n=2;
Sclerotiniaceae|Rep: Putative uncharacterized protein -
Botryotinia fuckeliana B05.10
Length = 323
Score = 35.5 bits (78), Expect = 1.0
Identities = 17/58 (29%), Positives = 30/58 (51%)
Frame = +3
Query: 324 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVIPQPTEEPGDKWIGFSVKE 497
V A++ +F+P GA L G +N + HL ++ +FN I + +W+G +E
Sbjct: 150 VTAEFLLFKPEKGAWLEGYINLQNEGHLGLVCWNLFNASIERARLPKEWRWVGVEDQE 207
>UniRef50_A3LV72 Cluster: DNA-dependent RNA polymerase I subunit
A43; n=2; Pichia|Rep: DNA-dependent RNA polymerase I
subunit A43 - Pichia stipitis (Yeast)
Length = 307
Score = 35.5 bits (78), Expect = 1.0
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +3
Query: 324 VQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
+ D+ I++P +G L G + + ++HL +L+H FN I
Sbjct: 122 ITVDFLIWRPQVGDVLEGYIYMQTASHLGLLIHDTFNASI 161
>UniRef50_Q00W27 Cluster: DNA polymerase epsilon, subunit C; n=3;
Ostreococcus|Rep: DNA polymerase epsilon, subunit C -
Ostreococcus tauri
Length = 569
Score = 34.3 bits (75), Expect = 2.3
Identities = 18/53 (33%), Positives = 27/53 (50%)
Frame = +3
Query: 285 GTIRNDNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVI 443
G + + + + V+AD +F P G++L G VNK + LV FNV I
Sbjct: 101 GRVLHASGHVDVCVEADCDVFTPVRGSRLVGTVNKIAHDFIGALVLDKFNVAI 153
>UniRef50_A0CSJ2 Cluster: Chromosome undetermined scaffold_26, whole
genome shotgun sequence; n=1; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_26,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 678
Score = 33.5 bits (73), Expect = 4.0
Identities = 25/89 (28%), Positives = 45/89 (50%), Gaps = 2/89 (2%)
Frame = +1
Query: 64 IIKFDLRELKQLANDKNSCLVVKKITQNLALQPWCLGNIKESITNLLDYKV--GKFDKEL 237
+ +FD+ ++ K L++ K T N+ LQ +C+ I I LL YK KF KE
Sbjct: 124 LTEFDIFIIRVQCLYKWGKLIMIKYTNNINLQRFCIQRILTEIKPLLHYKYFDEKFQKES 183
Query: 238 NGVLVSIKILVYFRMLALYAMIMRIYISK 324
+L I+ + + ++YA+ + ++K
Sbjct: 184 LNLLAKIREWL-AKEQSIYALCLSYKLTK 211
>UniRef50_Q55FA4 Cluster: RNA polymerase I subunit; n=1;
Dictyostelium discoideum AX4|Rep: RNA polymerase I
subunit - Dictyostelium discoideum AX4
Length = 356
Score = 32.7 bits (71), Expect = 7.1
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = +3
Query: 300 DNADIHFQVQADYFIFQPYIGAKLTGLVNKKCSTHLSVLVHRVFNVVIPQ 449
D+ + + + +F+P+ L G+V + +TH+S+LV + IP+
Sbjct: 79 DSPYLGMNIHVKFLVFKPFKDQILNGVVKRVSTTHISLLVFGTISASIPK 128
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 542,570,583
Number of Sequences: 1657284
Number of extensions: 9996755
Number of successful extensions: 21515
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 21044
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 21512
length of database: 575,637,011
effective HSP length: 97
effective length of database: 414,880,463
effective search space used: 43977329078
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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