BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0018 (727 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 102 5e-23 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 102 5e-23 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 70 3e-13 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 55 1e-08 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 44 3e-05 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 3e-05 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 40 3e-04 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.024 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.024 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.024 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 31 0.13 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.51 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 28 1.6 SPBC25H2.15 |||programmed cell death protein homolog|Schizosacch... 27 3.6 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 4.8 SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.3 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 25 8.3 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 8.3 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 8.3 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 8.3 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 102 bits (245), Expect = 5e-23 Identities = 47/70 (67%), Positives = 57/70 (81%) Frame = +3 Query: 51 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 230 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 231 KDEQDRCIPL 260 DEQ+R + + Sbjct: 61 ADEQERGVTI 70 Score = 89.8 bits (213), Expect = 3e-19 Identities = 50/85 (58%), Positives = 59/85 (69%), Gaps = 1/85 (1%) Frame = +2 Query: 257 IKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 433 IKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 434 ALXXXXXXXXXXXQTETVLRQAIAE 508 AL QTETVLRQA+ E Sbjct: 125 ALVVVDTIEGVCVQTETVLRQALGE 149 Score = 72.9 bits (171), Expect = 4e-14 Identities = 34/72 (47%), Positives = 46/72 (63%) Frame = +1 Query: 511 IKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGPMGEVLVDPSKGS 690 I+P++ +NK+DR YQ F R+VE+VNV+I+TY D +G+ V P KG+ Sbjct: 151 IRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDK--VLGDCQVFPDKGT 208 Query: 691 VGFGSGLHGWAF 726 V F SGLHGWAF Sbjct: 209 VAFASGLHGWAF 220 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 102 bits (245), Expect = 5e-23 Identities = 47/70 (67%), Positives = 57/70 (81%) Frame = +3 Query: 51 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTR 230 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+AG+ RF DTR Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTR 60 Query: 231 KDEQDRCIPL 260 DEQ+R + + Sbjct: 61 ADEQERGVTI 70 Score = 89.8 bits (213), Expect = 3e-19 Identities = 50/85 (58%), Positives = 59/85 (69%), Gaps = 1/85 (1%) Frame = +2 Query: 257 IKSTAISMFFELEEKDLVFITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDG 433 IKSTAIS+F E+ + D+ D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDG Sbjct: 70 IKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDG 124 Query: 434 ALXXXXXXXXXXXQTETVLRQAIAE 508 AL QTETVLRQA+ E Sbjct: 125 ALVVVDTIEGVCVQTETVLRQALGE 149 Score = 72.9 bits (171), Expect = 4e-14 Identities = 34/72 (47%), Positives = 46/72 (63%) Frame = +1 Query: 511 IKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGPMGEVLVDPSKGS 690 I+P++ +NK+DR YQ F R+VE+VNV+I+TY D +G+ V P KG+ Sbjct: 151 IRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDK--VLGDCQVFPDKGT 208 Query: 691 VGFGSGLHGWAF 726 V F SGLHGWAF Sbjct: 209 VAFASGLHGWAF 220 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 70.1 bits (164), Expect = 3e-13 Identities = 41/81 (50%), Positives = 54/81 (66%) Frame = +2 Query: 257 IKSTAISMFFELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 436 +KS+AIS+FF++ I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ DGA Sbjct: 70 MKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDGA 121 Query: 437 LXXXXXXXXXXXQTETVLRQA 499 QT TVLRQA Sbjct: 122 FVLVDAVEGVCSQTITVLRQA 142 Score = 64.1 bits (149), Expect = 2e-11 Identities = 29/64 (45%), Positives = 43/64 (67%) Frame = +3 Query: 69 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDR 248 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ AG RF D R+DE R Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITR 66 Query: 249 CIPL 260 I + Sbjct: 67 GITM 70 Score = 39.5 bits (88), Expect = 5e-04 Identities = 27/82 (32%), Positives = 36/82 (43%), Gaps = 10/82 (12%) Frame = +1 Query: 511 IKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATY----------NDDGGPMG 660 IK IL +NKMDR + R+VE VN +I T+ ND+ Sbjct: 147 IKVILVINKMDRLITELKLSPIEAHYHLLRLVEQVNAVIGTFYTGELMQLADNDEVISDE 206 Query: 661 EVLVDPSKGSVGFGSGLHGWAF 726 + P +G+V F S GWAF Sbjct: 207 GIYFAPEQGNVVFASAYDGWAF 228 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 54.8 bits (126), Expect = 1e-08 Identities = 28/59 (47%), Positives = 35/59 (59%) Frame = +2 Query: 320 NPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQ 496 N Q+ EK + IN+ID+PGH+DF+ EV ALRV DGA+ QT TV RQ Sbjct: 134 NEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQ 192 Score = 35.1 bits (77), Expect = 0.010 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +3 Query: 57 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 188 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 43.6 bits (98), Expect = 3e-05 Identities = 25/70 (35%), Positives = 40/70 (57%) Frame = +3 Query: 75 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCI 254 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I +F D K E +R Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI-NEHNFRNQFLD--KLEVERRR 104 Query: 255 PLNLRPSLCS 284 + ++ CS Sbjct: 105 GITVKAQTCS 114 Score = 39.1 bits (87), Expect = 6e-04 Identities = 15/31 (48%), Positives = 23/31 (74%) Frame = +2 Query: 347 KGFLINLIDSPGHVDFSSEVTAALRVTDGAL 439 + +L+NLID+PGHVDF +EV +L +G + Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCI 152 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 43.6 bits (98), Expect = 3e-05 Identities = 21/47 (44%), Positives = 28/47 (59%) Frame = +2 Query: 359 INLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQA 499 INLID+PGH DF+ EV ++ V DGA+ QT+ V +QA Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQA 141 Score = 35.1 bits (77), Expect = 0.010 Identities = 20/57 (35%), Positives = 31/57 (54%), Gaps = 3/57 (5%) Frame = +3 Query: 102 NIRNMSVIAHVDHGKSTLTDSLVSKAGI---IAGARAGETRFTDTRKDEQDRCIPLN 263 +IRN+ +IAH+D GK+TLT+ ++ G G+T D E+ R I +N Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGGFTSHFGNVDTGDT-VMDYLPAERQRGITIN 82 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 40.3 bits (90), Expect = 3e-04 Identities = 18/52 (34%), Positives = 26/52 (50%) Frame = +2 Query: 347 KGFLINLIDSPGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRQAI 502 K F ID+PGHVDF EV A + ++DG + T +++ AI Sbjct: 207 KTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAI 258 Score = 25.8 bits (54), Expect = 6.3 Identities = 18/69 (26%), Positives = 29/69 (42%) Frame = +1 Query: 520 ILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGPMGEVLVDPSKGSVGF 699 +L +NK+DR Y + +++ VN I + D + V P G+V F Sbjct: 265 VLVLNKVDRLILELRLPPNDAYHKLRHVIDEVNDNICQISKD----LKYRVSPELGNVCF 320 Query: 700 GSGLHGWAF 726 S G+ F Sbjct: 321 ASCDLGYCF 329 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.024 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 87 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 182 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.024 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 87 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 182 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.024 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +3 Query: 87 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 182 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 31.5 bits (68), Expect = 0.13 Identities = 18/57 (31%), Positives = 30/57 (52%) Frame = +3 Query: 93 KKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCIPLN 263 KK ++ N+ I HVDHGK+TLT ++ + A + D +E+ R I ++ Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITIS 105 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 29.5 bits (63), Expect = 0.51 Identities = 20/87 (22%), Positives = 41/87 (47%) Frame = -2 Query: 525 QNRLDASAIA*RSTVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPF 346 Q + +S+++ ++ S + + +TTT +PS + S++ +S S+ S Sbjct: 124 QTTVSSSSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSS 183 Query: 345 SLFSRWSGFVMNTKSFSSSSKNIEMAV 265 S S S ++ S SSSS + + + Sbjct: 184 SSSSSSSSSSSSSSSSSSSSSSSSVPI 210 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.9 bits (59), Expect = 1.6 Identities = 13/34 (38%), Positives = 22/34 (64%) Frame = +3 Query: 114 MSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETR 215 ++++ HVDHGK+TL D+ K+ I + G T+ Sbjct: 174 VTLMGHVDHGKTTLLDAF-RKSTIASTEHGGITQ 206 >SPBC25H2.15 |||programmed cell death protein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 26.6 bits (56), Expect = 3.6 Identities = 16/82 (19%), Positives = 39/82 (47%) Frame = +3 Query: 105 IRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCIPLNLRPSLCS 284 +++ I+H++ KS+ + V+ I + + G F+D + ++ PS S Sbjct: 112 VKSPKAISHLEEKKSSPKEKKVNPFAITSESSRGLNPFSDATSANNPFSLSTDVNPSKPS 171 Query: 285 SSLKRKI*YSSQTLTSVKRVRK 350 S++ K ++++ S+ +K Sbjct: 172 SNVFSKPSFAAKAQQSITDQQK 193 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 4.8 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +3 Query: 111 NMSVIAHVDHGKSTLTDSLVSKAGII 188 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPCC1322.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 262 Score = 25.4 bits (53), Expect = 8.3 Identities = 19/70 (27%), Positives = 33/70 (47%) Frame = -2 Query: 489 STVSVCTHTPDTQSTTTRAPSVTRSAAVTSEEKSTCPGESIKLIKKPFSLFSRWSGFVMN 310 S+ S T TP + STT+ + S + S ++S S+ + + S S S + Sbjct: 140 SSTSSSTATPSSSSTTSSSSSSSSSTPISSSITSSISSSASSSVSSS-SASSSGSISSAD 198 Query: 309 TKSFSSSSKN 280 K+ S+SS + Sbjct: 199 AKTVSASSNS 208 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 1/33 (3%) Frame = -2 Query: 351 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLM 256 P S +S W+ V+NTK SFS ++M +L+ Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELI 199 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 8.3 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -3 Query: 107 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 12 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 8.3 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -2 Query: 102 SASCPSSHGSRLP*NSPF*MVYDFCSIK 19 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.4 bits (53), Expect = 8.3 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = +1 Query: 136 ITASQPSRTRWFPR-PVSLLVREPERPVSLTRVRTNK 243 +T S T + P P S + REP P+S R+R+++ Sbjct: 48 LTPEPSSNTFYAPSSPASAVRREPLSPMSFVRMRSHR 84 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,034,745 Number of Sequences: 5004 Number of extensions: 60624 Number of successful extensions: 216 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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