BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= e40h0017 (797 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 151 1e-37 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 95 1e-20 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 83 3e-17 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 81 2e-16 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 74 3e-14 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 72 8e-14 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 70 4e-13 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 69 6e-13 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 66 5e-12 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 63 5e-11 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 62 1e-10 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 52 1e-07 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 47 3e-06 SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 32 0.083 SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 30 0.33 SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 1.8 SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 26 5.4 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 151 bits (366), Expect = 1e-37 Identities = 62/88 (70%), Positives = 75/88 (85%) Frame = +2 Query: 245 IEFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLS 424 ++ M W+ IPGK T WEGGLYKL M F ++YP+ PPKC+F PPLFHPNVYPSGTVCLS Sbjct: 36 LDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLS 95 Query: 425 LLDEEKDWRPAITIKQILLGIQDLLNEP 508 +L+EE+ W+PAITIKQILLGIQDLL++P Sbjct: 96 ILNEEEGWKPAITIKQILLGIQDLLDDP 123 Score = 57.2 bits (132), Expect = 3e-09 Identities = 23/38 (60%), Positives = 28/38 (73%) Frame = +3 Query: 141 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNL 254 MS + RL EERK WR+DHPFGF A+P K+ DG L+L Sbjct: 1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDL 38 Score = 29.9 bits (64), Expect = 0.44 Identities = 10/22 (45%), Positives = 18/22 (81%) Frame = +1 Query: 511 VKDPAQAEAYTIYCQNRLEYDK 576 + PAQ EAYT++ ++++EY+K Sbjct: 125 IASPAQTEAYTMFKKDKVEYEK 146 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 94.7 bits (225), Expect = 1e-20 Identities = 44/98 (44%), Positives = 56/98 (57%), Gaps = 11/98 (11%) Frame = +2 Query: 248 EFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 427 +F TW+C I G GTP+EGGLY + F DYP PP KFE FHPNVY GTVC+S+ Sbjct: 34 DFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISI 93 Query: 428 LDEEKD-----------WRPAITIKQILLGIQDLLNEP 508 L D W P ++++ILL + +L EP Sbjct: 94 LHAPGDDPNMYESSSERWSPVQSVEKILLSVMSMLAEP 131 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 83.4 bits (197), Expect = 3e-17 Identities = 37/85 (43%), Positives = 49/85 (57%) Frame = +2 Query: 254 MTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLD 433 M W I G TP+E G +KL + F + YP+ PP KF +FHPNVY +G +CL +L Sbjct: 34 MLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDIL- 92 Query: 434 EEKDWRPAITIKQILLGIQDLLNEP 508 + W P + IL IQ LLN+P Sbjct: 93 -QNRWSPTYDVAAILTSIQSLLNDP 116 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 80.6 bits (190), Expect = 2e-16 Identities = 33/83 (39%), Positives = 52/83 (62%) Frame = +2 Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439 W+ I G +P+ GG++ L + F DYP PPK F ++HPN+ +G++CL +L ++ Sbjct: 33 WQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQ 92 Query: 440 KDWRPAITIKQILLGIQDLLNEP 508 W PA+TI ++LL I LL +P Sbjct: 93 --WSPALTISKVLLSICSLLTDP 113 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 73.7 bits (173), Expect = 3e-14 Identities = 32/83 (38%), Positives = 50/83 (60%) Frame = +2 Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439 ++ + G + + +EGG + L + D+YP PP +F ++HPNV G +CLS L + Sbjct: 34 FKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLSTL--K 91 Query: 440 KDWRPAITIKQILLGIQDLLNEP 508 KDW PA+ I+ +LL IQ L+ P Sbjct: 92 KDWSPALQIRTVLLSIQALMGAP 114 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 72.1 bits (169), Expect = 8e-14 Identities = 34/83 (40%), Positives = 47/83 (56%) Frame = +2 Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439 W I G T +EG +K+ M F +YP SPP F P++HPNV SG +CL +L ++ Sbjct: 61 WAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDILKDK 120 Query: 440 KDWRPAITIKQILLGIQDLLNEP 508 W ++ ILL +Q LL EP Sbjct: 121 --WSAVYNVQTILLSLQSLLGEP 141 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 69.7 bits (163), Expect = 4e-13 Identities = 34/94 (36%), Positives = 51/94 (54%), Gaps = 11/94 (11%) Frame = +2 Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLL--- 430 WE I G + T +EGG + + F DYP PPK KF ++HPNV+P+G VC+S+L Sbjct: 38 WEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPP 97 Query: 431 --------DEEKDWRPAITIKQILLGIQDLLNEP 508 D + W P + + IL+ + +L+ P Sbjct: 98 GDDKYGYEDAGERWLPVHSPETILISVISMLSSP 131 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 69.3 bits (162), Expect = 6e-13 Identities = 31/78 (39%), Positives = 47/78 (60%), Gaps = 1/78 (1%) Frame = +2 Query: 278 GKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRP 454 G +GTP+EGG + + + DYP PPK F+ ++HPNV +G +CL +L ++ W P Sbjct: 42 GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSP 99 Query: 455 AITIKQILLGIQDLLNEP 508 T+K L+ +Q LL P Sbjct: 100 VYTMKSALISLQSLLCTP 117 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 66.1 bits (154), Expect = 5e-12 Identities = 28/71 (39%), Positives = 43/71 (60%) Frame = +2 Query: 296 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 475 ++GG +K R+ D+YP PPK K ++HPN+ G VCL++L +DW P + + I Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122 Query: 476 LLGIQDLLNEP 508 L+G+Q L P Sbjct: 123 LVGLQFLFLSP 133 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 62.9 bits (146), Expect = 5e-11 Identities = 28/83 (33%), Positives = 46/83 (55%) Frame = +2 Query: 260 WECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEE 439 W C G + + GG + + F DYP PP +F ++HPN G VCL++L ++ Sbjct: 39 WACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAIL-KQ 97 Query: 440 KDWRPAITIKQILLGIQDLLNEP 508 + ++P+I ++ +L I LL EP Sbjct: 98 QVFKPSIKLRSVLEQILQLLREP 120 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 61.7 bits (143), Expect = 1e-10 Identities = 29/87 (33%), Positives = 48/87 (55%), Gaps = 1/87 (1%) Frame = +2 Query: 245 IEFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 421 ++ W+ I G TP+EGG + L + + YP SPP F+ + HPN+ + +G VC+ Sbjct: 32 VDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCM 91 Query: 422 SLLDEEKDWRPAITIKQILLGIQDLLN 502 +L + W PA +++ L I LL+ Sbjct: 92 DIL--KTHWSPAWSLQSACLAIISLLS 116 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 51.6 bits (118), Expect = 1e-07 Identities = 27/79 (34%), Positives = 37/79 (46%), Gaps = 1/79 (1%) Frame = +2 Query: 254 MTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLD 433 + W I G TP+EGG Y +IF DYP PP + P + +CLS D Sbjct: 35 LEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSFSD 92 Query: 434 -EEKDWRPAITIKQILLGI 487 K W P+ + IL+G+ Sbjct: 93 FHPKSWNPSWMVSTILVGL 111 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 47.2 bits (107), Expect = 3e-06 Identities = 25/79 (31%), Positives = 41/79 (51%), Gaps = 2/79 (2%) Frame = +2 Query: 278 GKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP-SGTVCLSLLDEEKDWRP 454 G TP+ GG++K+ + +YP P F +FHPN+ SG+VCL ++++ W P Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQ--TWSP 95 Query: 455 AITIKQIL-LGIQDLLNEP 508 + I + + LL P Sbjct: 96 MFDMINIFEVFLPQLLRYP 114 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 32.3 bits (70), Expect = 0.083 Identities = 26/97 (26%), Positives = 41/97 (42%), Gaps = 1/97 (1%) Frame = +2 Query: 245 IEFMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCL 421 I W I G + E +Y L++ +YP +PP F + P V +G V Sbjct: 36 ITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNP 95 Query: 422 SLLDEEKDWRPAITIKQILLGIQDLLNEPT*KIQRKL 532 +D + W+ +++ +LL DL E RKL Sbjct: 96 HKIDCLRHWKREYSMETVLL---DLKKEMASSSNRKL 129 >SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 509 Score = 30.3 bits (65), Expect = 0.33 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = -3 Query: 369 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQVINS 247 N F G+ L + S SP HG P +PG+ H+ + NS Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASLSNS 482 >SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 27.9 bits (59), Expect = 1.8 Identities = 12/36 (33%), Positives = 22/36 (61%) Frame = -2 Query: 199 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 92 W LR+ +S++ + ++F K NSIY +F++ L Sbjct: 71 WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106 >SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 26.2 bits (55), Expect = 5.4 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +2 Query: 194 GPSLWFCREAYEESRWFIEFMTWECAIPGKKGTPWEGGLYK 316 GPS R+ + + ++ TW C++ G+ G +G L+K Sbjct: 122 GPSKVVSRK-HAVVEYDLDDQTWNCSVYGRNGIKVDGKLFK 161 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,332,181 Number of Sequences: 5004 Number of extensions: 73073 Number of successful extensions: 171 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 156 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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