Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= e40h0010
(773 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD... 138 5e-33
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 91 7e-19
At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate dehy... 39 0.004
At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate dehy... 37 0.013
At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate dehy... 37 0.013
At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein... 30 1.5
At5g55120.1 68418.m06871 expressed protein strong similarity to ... 30 2.0
At5g43870.1 68418.m05363 expressed protein 29 2.6
At4g26850.1 68417.m03865 expressed protein 29 2.6
At2g25170.1 68415.m03010 chromatin remodeling factor CHD3 (PICKL... 29 2.6
At1g06100.1 68414.m00639 fatty acid desaturase family protein si... 29 2.6
At4g23460.1 68417.m03381 beta-adaptin, putative strong similarit... 29 4.5
At2g01290.1 68415.m00043 expressed protein 28 6.0
At1g06090.1 68414.m00638 fatty acid desaturase family protein si... 28 7.9
>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
/ GPDH strong similarity to SP|P52425
Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
{Cuphea lanceolata}; contains Pfam profile PF01210:
NAD-dependent glycerol-3-phosphate dehydrogenase
Length = 400
Score = 138 bits (333), Expect = 5e-33
Identities = 75/170 (44%), Positives = 100/170 (58%), Gaps = 2/170 (1%)
Frame = +1
Query: 4 KLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIAEG 183
KL NVVA PD+ A KDA++L+FV PHQF+ IC L GKI A+SL+KG ++ +
Sbjct: 120 KLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDGKITGDVEAISLVKGMEVKKE 179
Query: 184 GWHRSYITYYYKMPKNSLCCI-NGSNIASEVAEEKFCETTIGCRDVM-LAPLMRDIIQTD 357
G I+ CC+ G+NIA+E+A EKF E T+G R +A + T
Sbjct: 180 G--PCMISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYRGSREIADTWVQLFSTP 237
Query: 358 YFRXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAVIRLGLME 507
YF +CG LKN+VA+ AGFVDGL G+NTKAA++R+GL E
Sbjct: 238 YFMVTPVHDVEGVELCGTLKNVVAIAAGFVDGLEMGNNTKAAIMRIGLRE 287
Score = 95.1 bits (226), Expect = 5e-20
Identities = 50/81 (61%), Positives = 56/81 (69%), Gaps = 2/81 (2%)
Frame = +3
Query: 525 VFYPGSKLSTFFESCGVADLITTCYGGRNRRVAEAFVKT--GRSIKELEDEMLNGQKLQG 698
+ +P K STFFESCGVAD+ITTC GGRNRRVAEAF K+ RS ELE EML GQKLQG
Sbjct: 294 LLFPSVKDSTFFESCGVADVITTCLGGRNRRVAEAFAKSRGKRSFDELEAEMLQGQKLQG 353
Query: 699 PITAEEVNHMLANKNMENKFP 761
TA EV +L + FP
Sbjct: 354 VSTAREVYEVLKHCGWLEMFP 374
>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein low similarity to SP|Q26756
Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
(EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 420
Score = 91.1 bits (216), Expect = 7e-19
Identities = 50/169 (29%), Positives = 78/169 (46%)
Frame = +1
Query: 1 HKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIKGFDIAE 180
HKLP NV+A D A DAD + VP QF + + + P +SL KG ++
Sbjct: 141 HKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYVDPGLPFISLSKGLELNT 200
Query: 181 GGWHRSYITYYYKMPKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDY 360
I K P+ ++G + A E+ + +D LA ++ ++ + Y
Sbjct: 201 LRMMSQIIPIALKNPRQPFVALSGPSFALELMNNLPTAMVVASKDKKLANAVQQLLASSY 260
Query: 361 FRXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAVIRLGLME 507
R I GALKN++A+ AG VDG+ G+N+ AA++ G E
Sbjct: 261 LRINTSSDVTGVEIAGALKNVLAIAAGIVDGMNLGNNSMAALVSQGCSE 309
>At3g07690.1 68416.m00923 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 455
Score = 38.7 bits (86), Expect = 0.004
Identities = 43/162 (26%), Positives = 70/162 (43%), Gaps = 9/162 (5%)
Frame = +1
Query: 28 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIAEGGWHRS 198
V ++ EA DAD++I +P + + + K A +SL KG + AE H
Sbjct: 141 VTNLQEAVWDADIVINGLPSTETFQVFNEISKYWKERVNAPVIISLAKGVE-AEFEPHPR 199
Query: 199 YITYYYKM------PKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDY 360
+T + P ++ + G NIASEV +++ I + PL + + Q+ +
Sbjct: 200 IVTPTQMIHRATGIPLENILYLGGPNIASEVYNKEYANARICGSEKWRKPLGKFLRQS-H 258
Query: 361 FRXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAV 486
F + G LKN+ A+GAG V L T +V
Sbjct: 259 FIVWDNSDLITHEVMGGLKNVYAIGAGMVATLTKESATSKSV 300
>At2g41540.2 68415.m05134 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 37.1 bits (82), Expect = 0.013
Identities = 39/161 (24%), Positives = 67/161 (41%), Gaps = 8/161 (4%)
Frame = +1
Query: 28 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIA-EGGWH- 192
V ++ EA DAD+++ +P R + + K +SL KG + A E H
Sbjct: 149 VTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETALEPVPHI 208
Query: 193 ---RSYITYYYKMPKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 363
I +P +++ + G NIA+E+ +++ I PL + ++ +F
Sbjct: 209 ITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLRQPHF 267
Query: 364 RXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAV 486
+ G LKN+ A+GAG V L T +V
Sbjct: 268 IVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSV 308
>At2g41540.1 68415.m05133 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein weak similarity to
SP|P46919 Glycerol-3-phosphate dehydrogenase [NAD(P)+]
(EC 1.1.1.94) {Bacillus subtilis}; contains Pfam profile
PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 462
Score = 37.1 bits (82), Expect = 0.013
Identities = 39/161 (24%), Positives = 67/161 (41%), Gaps = 8/161 (4%)
Frame = +1
Query: 28 VPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKIKPTAAA---LSLIKGFDIA-EGGWH- 192
V ++ EA DAD+++ +P R + + K +SL KG + A E H
Sbjct: 149 VTNLQEAVWDADIVVNGLPSTETREVFEEISKYWKERITVPIIISLSKGIETALEPVPHI 208
Query: 193 ---RSYITYYYKMPKNSLCCINGSNIASEVAEEKFCETTIGCRDVMLAPLMRDIIQTDYF 363
I +P +++ + G NIA+E+ +++ I PL + ++ +F
Sbjct: 209 ITPTKMIHQATGVPIDNVLYLGGPNIAAEIYNKEYANARICGAAKWRKPLAK-FLRQPHF 267
Query: 364 RXXXXXXXXXXXICGALKNIVAVGAGFVDGLGYGDNTKAAV 486
+ G LKN+ A+GAG V L T +V
Sbjct: 268 IVWDNSDLVTHEVMGGLKNVYAIGAGMVAALTNESATSKSV 308
>At2g15180.1 68415.m01731 zinc knuckle (CCHC-type) family protein
contains Pfam domain PF00098: Zinc knuckle
Length = 474
Score = 30.3 bits (65), Expect = 1.5
Identities = 12/40 (30%), Positives = 17/40 (42%)
Frame = +2
Query: 362 SGSWWWTMRTQSKYVER*RTLWQWVRVSWMASATVITQRL 481
S WWW +++ ER + W R+ W A Q L
Sbjct: 151 SALWWWDQDEYNRWYERRAPIRTWERLKWNMCAKYSPQSL 190
>At5g55120.1 68418.m06871 expressed protein strong similarity to
unknown protein (pir||T04808)
Length = 431
Score = 29.9 bits (64), Expect = 2.0
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = -3
Query: 237 QGIFRHLVIICDIRSMPATFGYIKSLNQ 154
+G+FR+ V C+ + +P +G+I LN+
Sbjct: 78 RGLFRYDVTACETKVIPGKYGFIAQLNE 105
>At5g43870.1 68418.m05363 expressed protein
Length = 453
Score = 29.5 bits (63), Expect = 2.6
Identities = 15/57 (26%), Positives = 27/57 (47%)
Frame = +2
Query: 350 RQTTSGSWWWTMRTQSKYVER*RTLWQWVRVSWMASATVITQRLLSSDSVSWRDQIR 520
+ T G WW K E +++ +W ASAT +++ +++S S Q+R
Sbjct: 4 KSKTIGEQWWRSNPTFKPPETPLDSMEFLSRTWSASATEVSRAVVASPPTSQPPQMR 60
>At4g26850.1 68417.m03865 expressed protein
Length = 442
Score = 29.5 bits (63), Expect = 2.6
Identities = 9/28 (32%), Positives = 19/28 (67%)
Frame = -3
Query: 237 QGIFRHLVIICDIRSMPATFGYIKSLNQ 154
+G+FR+ V C+ + +P +G++ LN+
Sbjct: 84 RGLFRYDVTACETKVIPGKYGFVAQLNE 111
>At2g25170.1 68415.m03010 chromatin remodeling factor CHD3 (PICKLE)
identical to chromatin remodeling factor CHD3
[Arabidopsis thaliana] GI:6478518
Length = 1384
Score = 29.5 bits (63), Expect = 2.6
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 642 GRSIKELEDEMLNGQKLQGPITAEEVNHMLANKN 743
G S E++DEML+G PIT+EE+ + N
Sbjct: 1220 GVSFIEVDDEMLDGLPKTDPITSEEIMGAAVDNN 1253
>At1g06100.1 68414.m00639 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase (ADS1)
GI:2970034 from [Arabidopsis thaliana]
Length = 299
Score = 29.5 bits (63), Expect = 2.6
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = -3
Query: 300 GRFAEFFLSNLRCNIAPINTAQGIFRHLVIICDIRSMPATFGYIKSLNQRQSSC-SWLYF 124
GR + +L C +AP N FR +I+ + ++ TF Y ++L R WL +
Sbjct: 34 GRASTVGTVHLLCLLAPFNYKWEAFRFGIILAILTNLCITFSYHRNLTHRSFKLPKWLEY 93
>At4g23460.1 68417.m03381 beta-adaptin, putative strong similarity
to SP|Q10567 Adapter-related protein complex 1 beta 1
subunit (Beta-adaptin 1) [Homo sapiens], beta-adaptin
[Drosophila melanogaster] GI:434902; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 893
Score = 28.7 bits (61), Expect = 4.5
Identities = 11/33 (33%), Positives = 22/33 (66%)
Frame = -1
Query: 329 GASITSRQPMVVSQNFSSATSDAILLPLIQHRE 231
GAS + PMV+SQN S+ ++ ++L +++ +
Sbjct: 733 GASARTMMPMVLSQNMSTGSTSSVLQVAVKNNQ 765
>At2g01290.1 68415.m00043 expressed protein
Length = 265
Score = 28.3 bits (60), Expect = 6.0
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1
Query: 409 ALKNIVAVGAGFVDGLGYGDNTKAAVIRLGLMERSNSL 522
A K + V +G V GLG G K AV R+G + R L
Sbjct: 41 AYKAVEFVESGMVLGLGTGSTAKHAVDRIGELLRQGKL 78
>At1g06090.1 68414.m00638 fatty acid desaturase family protein
similar to delta 9 acyl-lipid desaturase GB:BAA25180
GI:2970034 (ADS1) from [Arabidopsis thaliana]
Length = 299
Score = 27.9 bits (59), Expect = 7.9
Identities = 15/51 (29%), Positives = 24/51 (47%), Gaps = 1/51 (1%)
Frame = -3
Query: 273 NLRCNIAPINTAQGIFRHLVIICDIRSMPATFGYIKSLNQRQSSC-SWLYF 124
+L C +AP N R VI+ + S+ TF Y ++L + WL +
Sbjct: 43 HLLCLLAPFNYKWEALRFGVILAIVTSLSITFSYHRNLTHKSFKLPKWLEY 93
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,562,417
Number of Sequences: 28952
Number of extensions: 341339
Number of successful extensions: 953
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 922
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 948
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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