Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= cesb0095
         (491 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   218   4e-58
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   209   2e-55
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   115   3e-27
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    97   1e-21
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   3.5  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   3.5  
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom...    25   4.7  
SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2 |Schizos...    25   6.2  
SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac...    25   8.2  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  218 bits (532), Expect = 4e-58
 Identities = 95/141 (67%), Positives = 114/141 (80%)
 Frame = +3

Query: 69  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 248
           MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 249 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEILNLVLD 428
           +VPR+++VDLEP V+D+VRTG YR LFHP QLITGKEDA+NNYARGHYT+GKE+++ V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 429 IIHKLTYQCTILQRFLIFHSF 491
            I ++   C+ LQ FL+FHSF
Sbjct: 121 KIRRIADNCSGLQGFLVFHSF 141


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  209 bits (510), Expect = 2e-55
 Identities = 96/146 (65%), Positives = 116/146 (79%), Gaps = 5/146 (3%)
 Frame = +3

Query: 69  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 233
           MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 234 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEIL 413
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHP Q++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 414 NLVLDIIHKLTYQCTILQRFLIFHSF 491
           + VL+ I ++   C+ LQ FL+FHSF
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSF 145


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  115 bits (277), Expect = 3e-27
 Identities = 56/140 (40%), Positives = 78/140 (55%)
 Frame = +3

Query: 69  MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 248
           MRE + +  GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 249 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKEDAANNYARGHYTIGKEILNLVLD 428
           +VPRAV VDLEP  +D V++G +  LF P  +I G+  A N +A+GHYT G E+ + VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 429 IIHKLTYQCTILQRFLIFHS 488
           ++ +    C  LQ F + HS
Sbjct: 119 VVRREAEACDALQGFQLTHS 138


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 97.5 bits (232), Expect = 1e-21
 Identities = 51/141 (36%), Positives = 81/141 (57%), Gaps = 2/141 (1%)
 Frame = +3

Query: 72  RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 251
           RE I++  GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60

Query: 252 VPRAVFVDLEPTVVDEVRTGTYRQLFHPXQLITGKE--DAANNYARGHYTIGKEILNLVL 425
           +PRA+ +DLEP VV+ + + TY  L++P  ++  K    A NN+A G Y+  + I   ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 426 DIIHKLTYQCTILQRFLIFHS 488
           D+I +       L+ F + HS
Sbjct: 120 DMIDREADGSDSLEGFSLLHS 140


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 3.5
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +1

Query: 148 STASSLMARCPQTRPSGVETILSTLSSARPELAST 252
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 3.5
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 167 WPDAHRQDHRGWRRFFQHFLQR 232
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 4.7
 Identities = 15/41 (36%), Positives = 21/41 (51%)
 Frame = +1

Query: 58  LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 180
           L+S        +L+ P SR++ P  S +  STASSL    P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210


>SPAC23C4.12 |hhp2||serine/threonine protein kinase Hhp2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 400

 Score = 25.0 bits (52), Expect = 6.2
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = -1

Query: 122 ITDLDSGLANVYRDALTHFDLSF 54
           +T +D GLA  YRD  TH  + +
Sbjct: 146 VTMIDFGLAKKYRDFKTHVHIPY 168


>SPBC3H7.15 |hhp1||serine/threonine protein kinase
           Hhp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 365

 Score = 24.6 bits (51), Expect = 8.2
 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
 Frame = -1

Query: 176 HLAIRLDAVLQAV-KLPAGITDLDSGLANVYRDALTHFDLSF 54
           H  I+ D  L  + K    +  +D GLA  YRD  TH  + +
Sbjct: 128 HRDIKPDNFLMGIGKRGNQVNIIDFGLAKKYRDHKTHLHIPY 169


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,115,086
Number of Sequences: 5004
Number of extensions: 43550
Number of successful extensions: 128
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 125
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 192109570
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)