SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= cesb0091
         (530 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g55350.4 68414.m06326 calpain-type cysteine protease family i...   117   4e-27
At1g55350.3 68414.m06325 calpain-type cysteine protease family i...   117   4e-27
At1g55350.2 68414.m06324 calpain-type cysteine protease family i...   117   4e-27
At1g55350.1 68414.m06323 calpain-type cysteine protease family i...   117   4e-27
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil...    31   0.37 
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329...    30   0.85 
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329...    30   0.85 
At5g15870.1 68418.m01857 glycosyl hydrolase family 81 protein si...    30   0.85 
At3g45370.1 68416.m04898 hypothetical protein                          30   0.85 
At1g01790.1 68414.m00098 K+ efflux antiporter, putative (KEA1) i...    30   1.1  
At1g51220.1 68414.m05761 zinc finger (C2H2 type) protein (WIP5) ...    29   1.5  
At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29) c...    29   2.0  
At2g01610.1 68415.m00086 invertase/pectin methylesterase inhibit...    29   2.6  
At5g37670.1 68418.m04537 15.7 kDa class I-related small heat sho...    28   3.4  
At3g57785.1 68416.m06437 expressed protein                             28   4.5  
At3g07660.1 68416.m00918 expressed protein                             28   4.5  
At1g18310.1 68414.m02288 glycosyl hydrolase family 81 protein si...    28   4.5  
At5g45050.2 68418.m05524 disease resistance protein-related simi...    27   6.0  
At4g29060.1 68417.m04157 elongation factor Ts family protein sim...    27   6.0  
At4g00630.1 68417.m00087 K+ efflux antiporter, putative (KEA2) M...    27   7.9  
At3g18010.1 68416.m02289 homeobox-leucine zipper transcription f...    27   7.9  

>At1g55350.4 68414.m06326 calpain-type cysteine protease family
            identical to calpain-like protein GI:20268660 from
            [Arabidopsis thaliana]; contains Pfam profiles: PF00648
            Calpain family cysteine protease, PF01067 Calpain large
            subunit,domain III; identical to cDNA calpain-like
            protein GI:20268659
          Length = 2151

 Score =  117 bits (282), Expect = 4e-27
 Identities = 71/184 (38%), Positives = 105/184 (57%), Gaps = 10/184 (5%)
 Frame = +3

Query: 6    DVQQGELGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGVFHFRFWQYGRWVDVVID 185
            DV QG LGDCW L+AVA LT   ++   ++  +  ++E+  G++  RF   G WV VVID
Sbjct: 1752 DVCQGRLGDCWFLSAVAVLTEVSRISEVIITPE--YNEE--GIYTVRFCIQGEWVPVVID 1807

Query: 186  DRLPTYR-GKLVFLHSAEANEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVTEM 362
            D +P    GK  F  S + NE W +++EKAYAKLHGSYEAL+GG   +A+ D TGG  E 
Sbjct: 1808 DWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEE 1867

Query: 363  YEMSELPP-------NFYTILLKAYERNSLMGCSIE--PDPHILKAETPAGLIRGHAYSV 515
             ++              ++ LL+  +   L+G       D H+    + +G+++GHAYSV
Sbjct: 1868 IDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHV----SSSGIVQGHAYSV 1923

Query: 516  TRVK 527
             +V+
Sbjct: 1924 LQVR 1927


>At1g55350.3 68414.m06325 calpain-type cysteine protease family
            identical to calpain-like protein GI:20268660 from
            [Arabidopsis thaliana]; contains Pfam profiles: PF00648
            Calpain family cysteine protease, PF01067 Calpain large
            subunit,domain III; identical to cDNA calpain-like
            protein GI:20268659
          Length = 2151

 Score =  117 bits (282), Expect = 4e-27
 Identities = 71/184 (38%), Positives = 105/184 (57%), Gaps = 10/184 (5%)
 Frame = +3

Query: 6    DVQQGELGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGVFHFRFWQYGRWVDVVID 185
            DV QG LGDCW L+AVA LT   ++   ++  +  ++E+  G++  RF   G WV VVID
Sbjct: 1752 DVCQGRLGDCWFLSAVAVLTEVSRISEVIITPE--YNEE--GIYTVRFCIQGEWVPVVID 1807

Query: 186  DRLPTYR-GKLVFLHSAEANEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVTEM 362
            D +P    GK  F  S + NE W +++EKAYAKLHGSYEAL+GG   +A+ D TGG  E 
Sbjct: 1808 DWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEE 1867

Query: 363  YEMSELPP-------NFYTILLKAYERNSLMGCSIE--PDPHILKAETPAGLIRGHAYSV 515
             ++              ++ LL+  +   L+G       D H+    + +G+++GHAYSV
Sbjct: 1868 IDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHV----SSSGIVQGHAYSV 1923

Query: 516  TRVK 527
             +V+
Sbjct: 1924 LQVR 1927


>At1g55350.2 68414.m06324 calpain-type cysteine protease family
            identical to calpain-like protein GI:20268660 from
            [Arabidopsis thaliana]; contains Pfam profiles: PF00648
            Calpain family cysteine protease, PF01067 Calpain large
            subunit,domain III; identical to cDNA calpain-like
            protein GI:20268659
          Length = 2151

 Score =  117 bits (282), Expect = 4e-27
 Identities = 71/184 (38%), Positives = 105/184 (57%), Gaps = 10/184 (5%)
 Frame = +3

Query: 6    DVQQGELGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGVFHFRFWQYGRWVDVVID 185
            DV QG LGDCW L+AVA LT   ++   ++  +  ++E+  G++  RF   G WV VVID
Sbjct: 1752 DVCQGRLGDCWFLSAVAVLTEVSRISEVIITPE--YNEE--GIYTVRFCIQGEWVPVVID 1807

Query: 186  DRLPTYR-GKLVFLHSAEANEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVTEM 362
            D +P    GK  F  S + NE W +++EKAYAKLHGSYEAL+GG   +A+ D TGG  E 
Sbjct: 1808 DWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEE 1867

Query: 363  YEMSELPP-------NFYTILLKAYERNSLMGCSIE--PDPHILKAETPAGLIRGHAYSV 515
             ++              ++ LL+  +   L+G       D H+    + +G+++GHAYSV
Sbjct: 1868 IDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHV----SSSGIVQGHAYSV 1923

Query: 516  TRVK 527
             +V+
Sbjct: 1924 LQVR 1927


>At1g55350.1 68414.m06323 calpain-type cysteine protease family
            identical to calpain-like protein GI:20268660 from
            [Arabidopsis thaliana]; contains Pfam profiles: PF00648
            Calpain family cysteine protease, PF01067 Calpain large
            subunit,domain III; identical to cDNA calpain-like
            protein GI:20268659
          Length = 2151

 Score =  117 bits (282), Expect = 4e-27
 Identities = 71/184 (38%), Positives = 105/184 (57%), Gaps = 10/184 (5%)
 Frame = +3

Query: 6    DVQQGELGDCWLLAAVANLTLHRKLFFQVVPDDQSFDEDYAGVFHFRFWQYGRWVDVVID 185
            DV QG LGDCW L+AVA LT   ++   ++  +  ++E+  G++  RF   G WV VVID
Sbjct: 1752 DVCQGRLGDCWFLSAVAVLTEVSRISEVIITPE--YNEE--GIYTVRFCIQGEWVPVVID 1807

Query: 186  DRLPTYR-GKLVFLHSAEANEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVTEM 362
            D +P    GK  F  S + NE W +++EKAYAKLHGSYEAL+GG   +A+ D TGG  E 
Sbjct: 1808 DWIPCESPGKPAFATSRKLNELWVSMVEKAYAKLHGSYEALEGGLVQDALVDLTGGAGEE 1867

Query: 363  YEMSELPP-------NFYTILLKAYERNSLMGCSIE--PDPHILKAETPAGLIRGHAYSV 515
             ++              ++ LL+  +   L+G       D H+    + +G+++GHAYSV
Sbjct: 1868 IDLRSAQAQIDLASGRLWSQLLRFKQEGFLLGAGSPSGSDVHV----SSSGIVQGHAYSV 1923

Query: 516  TRVK 527
             +V+
Sbjct: 1924 LQVR 1927


>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
           protein / long-chain acyl-CoA synthetase family protein
           (LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
           LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
           HMM hit: AMP-binding enzymes PF00501
          Length = 691

 Score = 31.5 bits (68), Expect = 0.37
 Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
 Frame = +3

Query: 267 KAYAKLH-GSYEALKGGSTCEAMEDFTGGVTEMYEMSE 377
           K + KLH G YE L  G T EA+ DF  G+ ++   +E
Sbjct: 94  KTFEKLHLGDYEWLTFGKTLEAVCDFASGLVQIGHKTE 131


>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
           Protein of unknown function (DUF731)
          Length = 442

 Score = 30.3 bits (65), Expect = 0.85
 Identities = 22/82 (26%), Positives = 33/82 (40%), Gaps = 6/82 (7%)
 Frame = -2

Query: 409 FSRIV*KFGGSSDISYISVTPPVKSSMASQVEPPLRASYDP----WSFAYAFSSRALQNS 242
           FS  V   GG   ++ +SV PP+  +   +  PP +  Y P    W     F  + +Q  
Sbjct: 38  FSATVISGGGKKPLAKVSVKPPLNVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFG 97

Query: 241 FAS--AEWRNTSFPRYVGRRSS 182
             S    W  T   +  G +SS
Sbjct: 98  PCSDGKFWAGTVISQRSGSKSS 119


>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
           Protein of unknown function (DUF731)
          Length = 440

 Score = 30.3 bits (65), Expect = 0.85
 Identities = 22/82 (26%), Positives = 33/82 (40%), Gaps = 6/82 (7%)
 Frame = -2

Query: 409 FSRIV*KFGGSSDISYISVTPPVKSSMASQVEPPLRASYDP----WSFAYAFSSRALQNS 242
           FS  V   GG   ++ +SV PP+  +   +  PP +  Y P    W     F  + +Q  
Sbjct: 38  FSATVISGGGKKPLAKVSVKPPLNVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFG 97

Query: 241 FAS--AEWRNTSFPRYVGRRSS 182
             S    W  T   +  G +SS
Sbjct: 98  PCSDGKFWAGTVISQRSGSKSS 119


>At5g15870.1 68418.m01857 glycosyl hydrolase family 81 protein
           similar to beta-glucan-elicitor receptor GI:1752734 from
           [Glycine max]
          Length = 745

 Score = 30.3 bits (65), Expect = 0.85
 Identities = 10/36 (27%), Positives = 21/36 (58%)
 Frame = -2

Query: 379 SSDISYISVTPPVKSSMASQVEPPLRASYDPWSFAY 272
           + ++ Y+ V P +++ + + +EP L  S+ P  F Y
Sbjct: 401 AEEVCYLDVIPKIRTYLKNMIEPWLNGSFGPNGFLY 436


>At3g45370.1 68416.m04898 hypothetical protein
          Length = 224

 Score = 30.3 bits (65), Expect = 0.85
 Identities = 15/43 (34%), Positives = 18/43 (41%)
 Frame = +1

Query: 217 CSSTRPRRTSSGVLYSRRHMRNSTDRTKPSREARPARPWRTSP 345
           CSS  P      + + RRH+     R  P  E  P  PW  SP
Sbjct: 31  CSSPPPPEWLVDIGFIRRHLGVPLHRVIPLGEVEPTVPWMRSP 73


>At1g01790.1 68414.m00098 K+ efflux antiporter, putative (KEA1)
           identical to GB:AAD01191 GI:4101473 from [ Arabidopsis
           thaliana]; Monovalent cation:proton antiporter family 2
           (CPA2 family) member, PMID:11500563
          Length = 618

 Score = 29.9 bits (64), Expect = 1.1
 Identities = 20/46 (43%), Positives = 26/46 (56%), Gaps = 4/46 (8%)
 Frame = -1

Query: 437 AHKRVPLVRLQ--QDRVEVWRQFGHLVHFGDAAG-EVLHGL-AGRA 312
           + + +P V L    DRV + R     V+FGDA   EVLH + AGRA
Sbjct: 440 SERLIPFVALDVSSDRVTIGRSLDLPVYFGDAGSKEVLHKIGAGRA 485


>At1g51220.1 68414.m05761 zinc finger (C2H2 type) protein (WIP5)
           identical to WIP5 protein [Arabidopsis thaliana]
           gi|18376498|emb|CAC86167; contains Pfam domain, PF00096:
           Zinc finger, C2H2 type
          Length = 337

 Score = 29.5 bits (63), Expect = 1.5
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = -2

Query: 283 SFAYAFSSRALQNSFASAEWRNTSFPRYVGRRSSMTTSTQRP 158
           SF Y+ S   + NS  S  + NT+ P Y+    + +TS   P
Sbjct: 18  SFNYSTSLSYIYNSHGSYYYSNTTNPNYINHTHTTSTSPNSP 59


>At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29)
           contains Pfam profile PF02902: Ulp1 protease family,
           C-terminal catalytic domain; snoR29 gene for small
           nucleolar RNA GI:15706258
          Length = 917

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 12/25 (48%), Positives = 17/25 (68%)
 Frame = -3

Query: 279 SHMPSRVEHSRTRSPRPSGGTPVSL 205
           S+MPSR+  S +++P PSG T   L
Sbjct: 493 SNMPSRIADSSSKNPAPSGQTHAQL 517


>At2g01610.1 68415.m00086 invertase/pectin methylesterase inhibitor
           family protein low similarity to SP|Q42534
           Pectinesterase 2 precursor (EC 3.1.1.11) (Pectin
           methylesterase 2) (PE 2) {Arabidopsis thaliana};
           contains Pfam profile PF04043: Plant invertase/pectin
           methylesterase inhibitor
          Length = 222

 Score = 28.7 bits (61), Expect = 2.6
 Identities = 18/39 (46%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
 Frame = -1

Query: 494 DEPRRSLR-LEDV-GVGLNTAAHKRVPLVRLQQDRVEVW 384
           DE R SLR L D+ G G  TAA + V   R Q   V+ W
Sbjct: 129 DEMRGSLRQLRDMNGRGGGTAARRSVETFRFQMSNVQTW 167


>At5g37670.1 68418.m04537 15.7 kDa class I-related small heat shock
           protein-like (HSP15.7-CI) contains Pfam profile: PF00011
           Hsp20/alpha crystallin family; identified in Scharf,
           K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 137

 Score = 28.3 bits (60), Expect = 3.4
 Identities = 14/41 (34%), Positives = 20/41 (48%)
 Frame = +3

Query: 333 EDFTGGVTEMYEMSELPPNFYTILLKAYERNSLMGCSIEPD 455
           E F+GG +E     ELP N     +KAY  N ++   +  D
Sbjct: 80  EAFSGGGSEFLRRIELPENVKVDQVKAYVENGVLTVVVPKD 120


>At3g57785.1 68416.m06437 expressed protein
          Length = 114

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 14/39 (35%), Positives = 21/39 (53%)
 Frame = +1

Query: 220 SSTRPRRTSSGVLYSRRHMRNSTDRTKPSREARPARPWR 336
           +ST+P   ++  L +R H R+ +  T PSR A P    R
Sbjct: 2   ASTKPLIVAAKTLRNRIHFRSGSTSTGPSRWATPGHEER 40


>At3g07660.1 68416.m00918 expressed protein
          Length = 841

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 12/47 (25%), Positives = 20/47 (42%)
 Frame = -3

Query: 162 ARTARNGNGRRPHNLHRNSDHQVQLGRITCDEVSSWQRQPTANNRPT 22
           A +  N  G RP + + N  HQ    +     +  W+ +P  N+  T
Sbjct: 273 ASSHSNSTGSRPSSNYSNRSHQTVGPQRGAGSIKEWKPKPVVNHNTT 319


>At1g18310.1 68414.m02288 glycosyl hydrolase family 81 protein
           similar to beta-glucan binding protein GI:6625560 from
           [Phaseolus vulgaris]
          Length = 649

 Score = 27.9 bits (59), Expect = 4.5
 Identities = 10/36 (27%), Positives = 20/36 (55%)
 Frame = -2

Query: 379 SSDISYISVTPPVKSSMASQVEPPLRASYDPWSFAY 272
           + ++ Y+ V P + + + + +EP L  S+ P  F Y
Sbjct: 350 AEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLY 385


>At5g45050.2 68418.m05524 disease resistance protein-related similar
           to NL27 [Solanum tuberosum] GI:3947735; contains Pfam
           profiles PF03106: WRKY DNA -binding domain, PF00931:
           NB-ARC domain, PF00560: Leucine Rich Repeat
          Length = 1344

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 13/44 (29%), Positives = 23/44 (52%)
 Frame = +3

Query: 348 GVTEMYEMSELPPNFYTILLKAYERNSLMGCSIEPDPHILKAET 479
           G TE+    E+PPN  T+ L+    ++L    ++P   ++K  T
Sbjct: 647 GCTEIKSFPEIPPNIETLNLQGTGVSNLEQSDLKPLTSLMKIST 690


>At4g29060.1 68417.m04157 elongation factor Ts family protein
           similar to SP|P35019 Elongation factor Ts (EF-Ts)
           {Galdieria sulphuraria}; contains Pfam profiles PF00627:
           UBA/TS-N domain, PF00889: Elongation factor TS, PF00575:
           S1 RNA binding domain
          Length = 953

 Score = 27.5 bits (58), Expect = 6.0
 Identities = 17/45 (37%), Positives = 25/45 (55%)
 Frame = -1

Query: 263 E*STPELVRLGRVEEHQFPSVRWETVVDDHVHPAPVLPETEMEDA 129
           E S+PE +    VE+ Q  +   ET VD+   PAPV+ E   E++
Sbjct: 466 EVSSPEALASEEVEKEQVVA---ETPVDEVKTPAPVVTEASSEES 507


>At4g00630.1 68417.m00087 K+ efflux antiporter, putative (KEA2)
           Monovalent cation:proton antiporter family 2 (CPA2
           family) member, PMID:11500563; similar to
           SWISS-PROT:SPP03819 Glutathione-regulated
           potassium-efflux system protein kefC (K(+)/H(+)
           antiporter) [Escherichia coli]
          Length = 627

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 4/46 (8%)
 Frame = -1

Query: 437 AHKRVPLVRLQ--QDRVEVWRQFGHLVHFGDAAG-EVLHGL-AGRA 312
           + + +P V L    DRV + R     V+FGDA   EVLH + A RA
Sbjct: 448 SERLIPFVALDVSSDRVAIGRSLDLPVYFGDAGSREVLHKIGADRA 493


>At3g18010.1 68416.m02289 homeobox-leucine zipper transcription
           factor family protein similar to wuschel protein
           (GI:22087128) [Arabidopsis thaliana]; similar to HB2
           homeodomain protein GB:CAA09367 from [Populus tremula x
           Populus tremuloides]
          Length = 366

 Score = 27.1 bits (57), Expect = 7.9
 Identities = 9/29 (31%), Positives = 19/29 (65%)
 Frame = +3

Query: 348 GVTEMYEMSELPPNFYTILLKAYERNSLM 434
           G+   ++M +LPP+FY+     ++RN ++
Sbjct: 235 GINTTWQMMQLPPSFYSSSHHHHQRNLIL 263


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,496,576
Number of Sequences: 28952
Number of extensions: 280431
Number of successful extensions: 1035
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 970
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1022
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 987020800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -