BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= cesb0084 (510 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 209 2e-55 SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 148 6e-37 SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 36 0.004 SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 32 0.043 SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 30 0.18 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 0.71 SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 28 0.93 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 27 1.2 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 1.2 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 1.6 SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 26 2.9 SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre... 26 3.8 SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharo... 26 3.8 SPAP11E10.01 |||ornithine cyclodeaminase family |Schizosaccharom... 25 5.0 SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|... 25 5.0 SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|... 25 6.6 SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 25 6.6 SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 25 6.6 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 6.6 >SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 980 Score = 209 bits (510), Expect = 2e-55 Identities = 95/157 (60%), Positives = 120/157 (76%), Gaps = 1/157 (0%) Frame = +3 Query: 42 SYDWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVDKLELT 221 S+DW+R FTMD ++ RAV E F+RLH+ IYRANRLVNW L++ +S++EV+ +++ Sbjct: 232 SFDWTREAFTMDENLSRAVVETFVRLHEENIIYRANRLVNWCTALQTTLSNLEVENVDVP 291 Query: 222 GRTLLAIPGYEQKVEFGVLVHFAYKVEGSDEEVIVATTRVETMLGDVAVAVHPNDARYKH 401 GRTLL +PGY++ VE GVL AY VEGSDE +++ATTR ET+LGD AVAVHP D RYKH Sbjct: 292 GRTLLKVPGYDEPVEVGVLTSIAYAVEGSDERIVIATTRPETLLGDTAVAVHPQDPRYKH 351 Query: 402 LIGKNLIHPICNLKMPIIADE-YVDMAFGTGAVKITP 509 L GK + HP CN +PII D+ VDM FGTGAVKITP Sbjct: 352 LHGKFVKHPFCNRSIPIICDDIIVDMEFGTGAVKITP 388 >SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizosaccharomyces pombe|chr 1|||Manual Length = 950 Score = 148 bits (358), Expect = 6e-37 Identities = 77/157 (49%), Positives = 104/157 (66%), Gaps = 2/157 (1%) Frame = +3 Query: 45 YDWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVDKLELTG 224 +DW + +TMD + AVNEAFI L +AG IYRANR VNW L+SA+SDIEV+ ++ Sbjct: 160 FDWDNIFYTMDQNRSEAVNEAFISLFNAGLIYRANRFVNWCPKLESAVSDIEVESQQINK 219 Query: 225 RTLLAIPGYEQKVEFGVLVHFAYKVEGSD-EEVIVATTRVETMLGDVAVAVHPNDARYKH 401 + VEFG L +Y++EGSD E++ V+TTR ET+ GD A+AV P+D RYK Sbjct: 220 PVTKYVD--NTPVEFGWLYEISYQLEGSDNEQLNVSTTRPETIFGDRAIAVSPHDERYKK 277 Query: 402 LIGKNLIHP-ICNLKMPIIADEYVDMAFGTGAVKITP 509 +G+ + HP I +L +P+I D VD FGTG +KITP Sbjct: 278 YVGRFVKHPLIDDLLIPVICDNAVDRHFGTGVLKITP 314 >SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1064 Score = 35.9 bits (79), Expect = 0.004 Identities = 28/116 (24%), Positives = 54/116 (46%) Frame = +3 Query: 69 TMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVDKLELTGRTLLAIPG 248 T+ PS +V F LH G +YR R++ +S + +S+ E + Sbjct: 144 TLYPSFMESVWWVFKELHTKGKVYRGYRVMPYSTACTTPLSNFEAQQ------------N 191 Query: 249 YEQKVEFGVLVHFAYKVEGSDEEVIVATTRVETMLGDVAVAVHPNDARYKHLIGKN 416 Y++ + ++V F + + + TT T+ ++A+AVHP D +Y ++ K+ Sbjct: 192 YKEVPDPAIVVAF-QSISDPEVSFLAWTTTPWTLPSNLALAVHP-DLQYIKILDKD 245 >SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 973 Score = 32.3 bits (70), Expect = 0.043 Identities = 31/122 (25%), Positives = 53/122 (43%), Gaps = 4/122 (3%) Frame = +3 Query: 48 DWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAI--SDIEVDKLELT 221 DW TM A + F+ L I+R N+ V WS + +SA+ S+IE D ++ Sbjct: 175 DWPSRYITMSDKFEIAELKVFLSLLQKDLIFRQNKPVYWSSSSRSALAESEIEYDDNHVS 234 Query: 222 GRTLLAIPGYEQKVEFGVLVHFAYKVEGSDEEVIVATTRVETMLGDVAVAVHP--NDARY 395 P ++ E ++ + +V TT T+ ++A++ HP N Y Sbjct: 235 TSIYFTFPVNSFSID---------GCEYNNVKALVWTTTPWTIPSNLALSYHPEINYGLY 285 Query: 396 KH 401 +H Sbjct: 286 QH 287 >SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 874 Score = 30.3 bits (65), Expect = 0.18 Identities = 14/54 (25%), Positives = 26/54 (48%) Frame = +3 Query: 45 YDWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVD 206 +DW R T +P + F+++ G Y+A VNW + +++ +VD Sbjct: 144 FDWDREISTCNPDYYKWSQYLFLQMFHKGLAYQAEATVNWDPVDCTVLANEQVD 197 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 28.3 bits (60), Expect = 0.71 Identities = 21/64 (32%), Positives = 33/64 (51%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSM 194 +ATATS S+ ST + TSSS S+ T++ +T S + P+ S+ ST+ Sbjct: 205 SATATSSSLSSTAASNSATSSSLASSSLNS-TTSATATSSSISSTVSSSTPLTSSNSTTA 263 Query: 193 SEIA 182 + A Sbjct: 264 ATSA 267 Score = 24.6 bits (51), Expect = 8.7 Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 1/60 (1%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSS-EPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTS 197 T++ATS S+ ST + TSSS S+L + ++T S+ S +N +S+L++S Sbjct: 173 TSSATSSSLSSTAASNSATSSSLASSSLNSTTSATATSSSLSSTAASNSA--TSSSLASS 230 >SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 1563 Score = 27.9 bits (59), Expect = 0.93 Identities = 24/78 (30%), Positives = 32/78 (41%) Frame = -3 Query: 478 AMSTYSSAMMGILRLQIG*IKFLPIKCLYLASLGWTATATSPSMVSTLVVATITSSSEPS 299 A+ST++S +Q FLP S A S ST V T ++S P+ Sbjct: 118 AISTFTSGKEASYAIQASPSTFLPDSTTTSGSQVSNAVEAS----STFVADTTSTSCNPA 173 Query: 298 TLYAK*TSTPNSTFCSYP 245 T+ TS ST C P Sbjct: 174 TVLIVTTSGSTSTSCPPP 191 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 27.5 bits (58), Expect = 1.2 Identities = 21/69 (30%), Positives = 35/69 (50%) Frame = -3 Query: 397 LYLASLGWTATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPV 218 +YL G T S S VS+ + +SSS PS+ T++P+S+ S ++ Sbjct: 115 VYLTGNGVLQTTVSSSSVSS--TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSS 172 Query: 217 NSNLSTSMS 191 +S+ S+S S Sbjct: 173 SSSSSSSSS 181 Score = 24.6 bits (51), Expect = 8.7 Identities = 17/60 (28%), Positives = 33/60 (55%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSM 194 T T TSPS S+ ++ +SSS S+ + +S+ +S+ S ++ +S+ S+S+ Sbjct: 149 TTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSV 208 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 27.5 bits (58), Expect = 1.2 Identities = 18/52 (34%), Positives = 24/52 (46%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPV 218 T TS S S + T TSS++ ++ TST +T S P N PV Sbjct: 250 TVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPV 301 Score = 26.6 bits (56), Expect = 2.2 Identities = 21/65 (32%), Positives = 26/65 (40%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSM 194 T TS S ST TSS+ S+ TST +T S P N PV + + Sbjct: 358 TVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVPPTS 417 Query: 193 SEIAL 179 S L Sbjct: 418 SSTPL 422 Score = 25.4 bits (53), Expect = 5.0 Identities = 19/52 (36%), Positives = 22/52 (42%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPV 218 T TS S ST TSS+ S+ TST +T S P N PV Sbjct: 304 TVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPV 355 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 27.1 bits (57), Expect = 1.6 Identities = 20/70 (28%), Positives = 40/70 (57%), Gaps = 2/70 (2%) Frame = -3 Query: 394 YLASLGWTATATSPSMVS--TLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLP 221 YL+S ++++SPS S TL +++++SS PST + +++ + + S A+ Sbjct: 209 YLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASSSSS 268 Query: 220 VNSNLSTSMS 191 +S +S+S S Sbjct: 269 SSSIISSSSS 278 Score = 27.1 bits (57), Expect = 1.6 Identities = 14/61 (22%), Positives = 37/61 (60%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSM 194 +++++SP+ S+ + ++ +SSS S+ + + + +S+F S P ++ + +S+ +S Sbjct: 294 SSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSS 353 Query: 193 S 191 S Sbjct: 354 S 354 Score = 26.2 bits (55), Expect = 2.9 Identities = 18/59 (30%), Positives = 34/59 (57%) Frame = -3 Query: 367 TATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSMS 191 T+TS S +S+ V ++SSS PS+ + ++ + + S P ++ +S+LS+S S Sbjct: 203 TSTSSSYLSSSSV--VSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSS 259 Score = 25.0 bits (52), Expect = 6.6 Identities = 17/46 (36%), Positives = 28/46 (60%) Frame = -3 Query: 328 ATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSMS 191 AT TSSSEPS ++++ ++TP+ T +++ P S L +S S Sbjct: 76 ATPTSSSEPS-IFSE-SATPSETNSYSSPVSSYSDPATSQLPSSTS 119 Score = 25.0 bits (52), Expect = 6.6 Identities = 12/59 (20%), Positives = 37/59 (62%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTS 197 +++++SP+ S+ + ++ +SSS P++ + +S+ +S+ +++ + +S+ S+S Sbjct: 277 SSSSSSPTSTSSTISSSSSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSS 335 Score = 24.6 bits (51), Expect = 8.7 Identities = 17/65 (26%), Positives = 36/65 (55%) Frame = -3 Query: 391 LASLGWTATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNS 212 + S ++++TS S+ S+ +T +SSS S++ + +S+ +S + I++ +S Sbjct: 240 IPSTSSSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSS 299 Query: 211 NLSTS 197 STS Sbjct: 300 PTSTS 304 >SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 741 Score = 26.2 bits (55), Expect = 2.9 Identities = 21/67 (31%), Positives = 30/67 (44%) Frame = -3 Query: 325 TITSSSEPSTLYAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSMSEIALFNVQDQLTNRF 146 T S S PSTL TS N+ F S P + P+ SNL+++ + D T+ Sbjct: 284 TTPSDSRPSTL----TSLFNAFFSSMPDRPSSNEPMTSNLTSNSGSMTNSTSTDLPTSNL 339 Query: 145 AR*MSPA 125 +PA Sbjct: 340 PSQNAPA 346 >SPBC19C2.09 |sre1||sterol regulatory element binding protein Sre1|Schizosaccharomyces pombe|chr 2|||Manual Length = 900 Score = 25.8 bits (54), Expect = 3.8 Identities = 22/79 (27%), Positives = 32/79 (40%), Gaps = 5/79 (6%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTPN-----STFCSYPGIANRVLPVNSN 209 T T SP S V I SSS ++ TSTP + C I P +N Sbjct: 19 TPTKASPDSKSPNSVGAIPSSSPLASSTKASTSTPFVENCSNLLCDLASIVEDSPPTLTN 78 Query: 208 LSTSMSEIALFNVQDQLTN 152 S S +L +++ ++N Sbjct: 79 TSLSPHSFSLSDMESSMSN 97 >SPBC31F10.10c |||zf-MYND type zinc finger protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 574 Score = 25.8 bits (54), Expect = 3.8 Identities = 12/34 (35%), Positives = 20/34 (58%) Frame = -3 Query: 274 TPNSTFCSYPGIANRVLPVNSNLSTSMSEIALFN 173 TP++T P A+ P+NSN+S S+ ++ N Sbjct: 141 TPSTT--PPPSSASAPFPINSNISNSLDSVSQHN 172 >SPAP11E10.01 |||ornithine cyclodeaminase family |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 25.4 bits (53), Expect = 5.0 Identities = 20/59 (33%), Positives = 27/59 (45%), Gaps = 4/59 (6%) Frame = -2 Query: 479 SHVHIFISNDGHLEIANRMNQILAYQVFIPG----IIRVDSHSHIPKHGFYSSSSHYHL 315 + +H I D AN N +L +IPG + +V+ KHG S SSHY L Sbjct: 38 TRLHYPIDEDNPSSTAN--NILLIMPCWIPGKFLGVKQVNVFPENTKHGLPSLSSHYLL 94 >SPAC4A8.06c |||esterase/lipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 578 Score = 25.4 bits (53), Expect = 5.0 Identities = 8/25 (32%), Positives = 15/25 (60%) Frame = -2 Query: 395 IPGIIRVDSHSHIPKHGFYSSSSHY 321 +P ++ D+ +IP GF+ +S Y Sbjct: 251 LPSVVADDAADYIPSEGFHHRASRY 275 >SPAC17G8.02 |||uridine ribohydrolase |Schizosaccharomyces pombe|chr 1|||Manual Length = 330 Score = 25.0 bits (52), Expect = 6.6 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = -3 Query: 292 YAK*TSTPNSTFCSYPGIANRVLPVNSNLSTSMSEIAL 179 Y K +TP++ F Y I+N PV + ++ IAL Sbjct: 109 YPKKKATPDAVFAMYTTISNYPEPVTLVATGPLTNIAL 146 >SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 25.0 bits (52), Expect = 6.6 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = -3 Query: 409 PIKCLYLASLGWTATATSPSMVSTLVVATITSSSEPSTLY 290 PI+ LYL + T T P++VS + + + S T++ Sbjct: 514 PIRALYLIAKNGTPTLKKPNLVSKNLKSFLNSCLTIDTIF 553 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 25.0 bits (52), Expect = 6.6 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +3 Query: 42 SYDWSRVRFTMDPSMCRA 95 SY+W+R F S+CRA Sbjct: 913 SYNWARENFLQYKSLCRA 930 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 25.0 bits (52), Expect = 6.6 Identities = 22/66 (33%), Positives = 34/66 (51%), Gaps = 3/66 (4%) Frame = -3 Query: 373 TATATSPSMVSTLVVATITSSSEPSTLYAK*TSTP---NSTFCSYPGIANRVLPVNSNLS 203 +++ +SPS S +I+SSS ST + TS+P +S+ S I + P S+L Sbjct: 581 SSSTSSPSSTSL----SISSSSTSSTFSSASTSSPSSISSSISSSSTILSSPTPSTSSLM 636 Query: 202 TSMSEI 185 S S I Sbjct: 637 ISSSSI 642 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,078,838 Number of Sequences: 5004 Number of extensions: 40056 Number of successful extensions: 168 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 204242806 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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