SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= cesb0084
         (510 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA li...   199   8e-52
At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)...   141   2e-34
At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)...    38   0.005
At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative / leuc...    37   0.007
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d...    27   5.5  
At2g40970.1 68415.m05060 myb family transcription factor contain...    27   5.5  
At1g73110.1 68414.m08453 ribulose bisphosphate carboxylase/oxyge...    27   9.7  
At1g69450.1 68414.m07980 early-responsive to dehydration protein...    27   9.7  

>At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA
           ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA
           synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS)
           {Arabidopsis thaliana}
          Length = 1108

 Score =  199 bits (486), Expect = 8e-52
 Identities = 96/158 (60%), Positives = 117/158 (74%), Gaps = 1/158 (0%)
 Frame = +3

Query: 39  SSYDWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVDKLEL 218
           +S DWSR  FTMD    +AV EAF+RL+  G IYR  RLVNW C L++AISD+EV+ +++
Sbjct: 269 ASLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIRLVNWDCILRTAISDVEVEYIDI 328

Query: 219 TGRTLLAIPGYEQKVEFGVLVHFAYKVEGSDEEVIVATTRVETMLGDVAVAVHPNDARYK 398
             +TLL +PGYE+ VEFG+L  FAY +EG   EVIVATTRVETMLGD A+A+HP+DARYK
Sbjct: 329 KEKTLLKVPGYEKPVEFGLLTSFAYPLEGGLGEVIVATTRVETMLGDTAIAIHPDDARYK 388

Query: 399 HLIGKNLIHPICNLKMPIIADE-YVDMAFGTGAVKITP 509
           HL GK  +HP    K+PII D   VD  FGTG VKITP
Sbjct: 389 HLHGKFAVHPFNGRKLPIICDGILVDPNFGTGCVKITP 426


>At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)
           family protein similar to SP|P11931 Valyl-tRNA
           synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS)
           {Bacillus stearothermophilus}; contains Pfam profile
           PF00133: tRNA synthetases class I (I, L, M and V)
          Length = 970

 Score =  141 bits (342), Expect = 2e-34
 Identities = 75/158 (47%), Positives = 101/158 (63%), Gaps = 1/158 (0%)
 Frame = +3

Query: 39  SSYDWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVDKLEL 218
           +S DWSR RFT+D  + RAV EAF++LHD G IY+ + +VNWS  L++A+SD+EV+  E 
Sbjct: 200 ASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVNWSPNLQTAVSDLEVEYSE- 258

Query: 219 TGRTLLAIPGYEQKVEFGVLVHFAYKVEGSDEEVIVATTRVETMLGDVAVAVHPNDARYK 398
                          E G L H  Y+V GS + + +ATTR ET+ GDVA+AVHP D RY 
Sbjct: 259 ---------------EPGFLYHIKYRVAGSPDFLTIATTRPETLFGDVALAVHPEDDRYS 303

Query: 399 HLIGKNLIHPIC-NLKMPIIADEYVDMAFGTGAVKITP 509
             +G+  I P+     +PIIAD+YVD  FGTG +KI+P
Sbjct: 304 KYVGQTAIVPMTYGRHVPIIADKYVDKDFGTGVLKISP 341


>At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)
           family protein similar to SP|P41972 Isoleucyl-tRNA
           synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase)
           (IleRS) {Staphylococcus aureus}; contains Pfam profile
           PF00133: tRNA synthetases class I (I, L, M and V)
          Length = 1093

 Score = 37.5 bits (83), Expect = 0.005
 Identities = 17/53 (32%), Positives = 31/53 (58%)
 Frame = +3

Query: 48  DWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEVD 206
           DW+    T+DP    A  E F ++   G IYR  + V+WS + ++A+++ E++
Sbjct: 247 DWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELE 299


>At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative /
           leucine--tRNA ligase, putative similar to SP|P36430
           Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine--tRNA
           ligase) (LeuRS) {Bacillus subtilis}; contains Pfam
           profile PF00133: tRNA synthetases class I (I, L, M and
           V)
          Length = 973

 Score = 37.1 bits (82), Expect = 0.007
 Identities = 18/54 (33%), Positives = 28/54 (51%)
 Frame = +3

Query: 42  SYDWSRVRFTMDPSMCRAVNEAFIRLHDAGDIYRANRLVNWSCTLKSAISDIEV 203
           SYDW R   T +P   +     F++L+  G  Y+A   VNW   L + +++ EV
Sbjct: 201 SYDWDRELSTTEPDYYKWTQWIFLQLYKKGLAYQAEVPVNWCPALGTVLANEEV 254


>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
           domain PF04931: DNA polymerase V
          Length = 1306

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 14/27 (51%), Positives = 19/27 (70%)
 Frame = -3

Query: 442 LRLQIG*IKFLPIKCLYLASLGWTATA 362
           LRLQ   +KFL ++ L+LASLG   T+
Sbjct: 653 LRLQKQILKFLAVQGLFLASLGTEVTS 679


>At2g40970.1 68415.m05060 myb family transcription factor contains
           Pfam profile: PF00249 myb-like DNA-binding domain
          Length = 248

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 12/42 (28%), Positives = 22/42 (52%)
 Frame = -2

Query: 464 FISNDGHLEIANRMNQILAYQVFIPGIIRVDSHSHIPKHGFY 339
           F+   GHL I N + + +   + + G+ R +  SH+ K+  Y
Sbjct: 118 FVDAVGHLGIKNAVPKTIMQLMSVEGLTRENVASHLQKYRLY 159


>At1g73110.1 68414.m08453 ribulose bisphosphate
           carboxylase/oxygenase activase, putative / RuBisCO
           activase, putative similar to ribulose bisphosphate
           carboxylase/oxygenase activase, chloroplast precursor
           (RuBisCO activase, RA) [Oryza sativa] SWISS-PROT:P93431
          Length = 432

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = +3

Query: 327 ATTRVETMLGDVAVAVHPNDARYKHLIGKNLIHPICNLKMPII 455
           AT   E + GD  +A    D    H++ KN + P  N+K+P+I
Sbjct: 125 ATRSFEHLQGDYYIAPSFLDKVAVHIV-KNYLAPSLNIKIPLI 166


>At1g69450.1 68414.m07980 early-responsive to dehydration
           protein-related / ERD protein-related low similarity to
           ERD4 protein (early-responsive to dehydration stress)
           [Arabidopsis thaliana] GI:15375406; contains Pfam
           profile PF02714: Domain of unknown function DUF221
          Length = 646

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 13/32 (40%), Positives = 17/32 (53%)
 Frame = -3

Query: 394 YLASLGWTATATSPSMVSTLVVATITSSSEPS 299
           Y+ + GWT T T    V   +V+ I  S EPS
Sbjct: 427 YVVTTGWTDTLTELFRVVPFMVSYIKRSFEPS 458


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,987,503
Number of Sequences: 28952
Number of extensions: 213994
Number of successful extensions: 620
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 616
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -