BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= cesb0069 (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces ... 62 6e-11 SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pom... 58 2e-09 SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 36 0.007 SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|c... 31 0.12 SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||M... 31 0.21 SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 28 1.1 SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyc... 28 1.5 SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc... 26 5.9 SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation fac... 25 7.9 >SPCC1223.06 |tea1|alp8|cell end marker Tea1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1147 Score = 62.5 bits (145), Expect = 6e-11 Identities = 63/236 (26%), Positives = 91/236 (38%), Gaps = 11/236 (4%) Frame = +3 Query: 3 GEHPPSLQEHTATAHGHRIYVFGGEAG---ALTNETPLWIYDTETQIWRKLPGQVNYTSS 173 GE P H + G+ VFGG A + L++ +T + +W+K S Sbjct: 128 GETPSPRLGHASILIGNAFIVFGGLTNHDVADRQDNSLYLLNTSSLVWQKANA-----SG 182 Query: 174 VRRRARDPRGAVSGPRGRRGHTAHALKDCLLIYGGYQDLKGSTNELWAFHYE----SES- 338 R P GR GHT L + ++GG + L N+L F S+S Sbjct: 183 AR------------PSGRYGHTISCLGSKICLFGG-RLLDYYFNDLVCFDLNNLNTSDSR 229 Query: 339 WHQVRTWN-PGPARHRHAAALHDERLYVHGGQCDLRDCADLWFYDTMSRVWREV-XXXXX 512 W N P PAR H A ++LY+ GG DLW Y W +V Sbjct: 230 WELASVVNDPPPARAGHVAFTFSDKLYIFGGTDGANFFNDLWCYHPKQSAWSKVETFGVA 289 Query: 513 XXXXXXXXXXXXXXXLYIFGGDSHSHPH-NDLWRFHFATETWEHIPNTVKWPSPRT 677 LY+FGG + NDL+ F +++ W + + PSPR+ Sbjct: 290 PNPRAGHAASVVEGILYVFGGRASDGTFLNDLYAFRLSSKHWYKLSDLPFTPSPRS 345 Score = 46.8 bits (106), Expect = 3e-06 Identities = 51/231 (22%), Positives = 79/231 (34%), Gaps = 11/231 (4%) Frame = +3 Query: 30 HTATAHGHRIYVFGGEAGALTNETPLWIYDTETQIWRKLPGQVNYTSSVRRRARDPRGAV 209 H G IY+FGG A + LW+ + T + L R Sbjct: 86 HLYAEGGQEIYIFGGVASDSQPKNDLWVLNLATSQFTSL-----------------RSLG 128 Query: 210 SGPRGRRGHTAHALKDCLLIYGGYQD---LKGSTNELWAFHYESESWHQVRTWNPGPA-R 377 P R GH + + + +++GG + N L+ + S W + P+ R Sbjct: 129 ETPSPRLGHASILIGNAFIVFGGLTNHDVADRQDNSLYLLNTSSLVWQKANASGARPSGR 188 Query: 378 HRHAAALHDERLYVHGGQC------DLRDCADLWFYDTMSRVWREVXXXXXXXXXXXXXX 539 + H + ++ + GG+ DL C DL +T W Sbjct: 189 YGHTISCLGSKICLFGGRLLDYYFNDLV-CFDLNNLNTSDSRWELASVVNDPPPARAGHV 247 Query: 540 XXXXXX-LYIFGGDSHSHPHNDLWRFHFATETWEHIPNTVKWPSPRTDTCA 689 LYIFGG ++ NDLW +H W + P+PR A Sbjct: 248 AFTFSDKLYIFGGTDGANFFNDLWCYHPKQSAWSKVETFGVAPNPRAGHAA 298 Score = 37.5 bits (83), Expect = 0.002 Identities = 19/46 (41%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Frame = +3 Query: 558 LYIFGG-DSHSHPHNDLWRFHFATETWEHIPNTVKWPSPRTDTCAI 692 +YIFGG S S P NDLW + AT + + + + PSPR +I Sbjct: 95 IYIFGGVASDSQPKNDLWVLNLATSQFTSLRSLGETPSPRLGHASI 140 Score = 32.7 bits (71), Expect = 0.052 Identities = 26/98 (26%), Positives = 43/98 (43%), Gaps = 8/98 (8%) Frame = +3 Query: 225 RRGHTAHALKDC---LLIYGGYQDLKGSTNELWAFHYESESWHQVRTWNPGPA-RHRHAA 392 R H +H + + I+GG N+LW + + + +R+ P+ R HA+ Sbjct: 80 RYSHASHLYAEGGQEIYIFGGVASDSQPKNDLWVLNLATSQFTSLRSLGETPSPRLGHAS 139 Query: 393 ALHDERLYVHGG--QCDLRDCAD--LWFYDTMSRVWRE 494 L V GG D+ D D L+ +T S VW++ Sbjct: 140 ILIGNAFIVFGGLTNHDVADRQDNSLYLLNTSSLVWQK 177 >SPAC6G10.02c |tea3||cell end marker Tea3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1125 Score = 57.6 bits (133), Expect = 2e-09 Identities = 46/123 (37%), Positives = 60/123 (48%), Gaps = 3/123 (2%) Frame = +3 Query: 12 PPSLQEHTATAHGHRIYVFGG--EAGALTNETPLWIYDTETQIWRKLPGQVNYTSSVRRR 185 PP H+ T +I+V GG + G L++ LW++D ET W + VR Sbjct: 245 PPPRSNHSVTLVQGKIFVHGGHNDTGPLSD---LWLFDLETLSW----------TEVRSI 291 Query: 186 ARDPRGAVSGPRGRRGHTAHALKDCLLIYGGYQDLKGST-NELWAFHYESESWHQVRTWN 362 R P GPR GH A + D + IYGG +D KG NELWAF+Y + W V N Sbjct: 292 GRFP-----GPR--EGHQATTIDDTVYIYGG-RDNKGLILNELWAFNYSQQRWSLVS--N 341 Query: 363 PGP 371 P P Sbjct: 342 PIP 344 Score = 54.4 bits (125), Expect = 1e-08 Identities = 30/98 (30%), Positives = 51/98 (52%), Gaps = 6/98 (6%) Frame = +3 Query: 198 RGAVSGPRGRRGHTAHALKDCLLIYGGYQDLKGST-----NELWAFHYESESWHQVRTWN 362 RG + R GH+ D + ++GG ST N L+A++++S W+ V T + Sbjct: 104 RGEDNDSPARVGHSIVCSADTIYLFGGCDSETDSTFEVGDNSLYAYNFKSNQWNLVSTQS 163 Query: 363 PGPA-RHRHAAALHDERLYVHGGQCDLRDCADLWFYDT 473 P P+ R H+ L D +L++ GG+C + D+ +DT Sbjct: 164 PLPSPRTGHSMLLVDSKLWIFGGECQGKYLNDIHLFDT 201 Score = 54.0 bits (124), Expect = 2e-08 Identities = 27/98 (27%), Positives = 44/98 (44%), Gaps = 2/98 (2%) Frame = +3 Query: 369 PARHRHAAALHDERLYVHGGQCDLRDCADLWFYDTMSRVWREV-XXXXXXXXXXXXXXXX 545 P R H+ L +++VHGG D +DLW +D + W EV Sbjct: 246 PPRSNHSVTLVQGKIFVHGGHNDTGPLSDLWLFDLETLSWTEVRSIGRFPGPREGHQATT 305 Query: 546 XXXXLYIFGG-DSHSHPHNDLWRFHFATETWEHIPNTV 656 +YI+GG D+ N+LW F+++ + W + N + Sbjct: 306 IDDTVYIYGGRDNKGLILNELWAFNYSQQRWSLVSNPI 343 Score = 35.5 bits (78), Expect = 0.007 Identities = 12/39 (30%), Positives = 23/39 (58%) Frame = +3 Query: 558 LYIFGGDSHSHPHNDLWRFHFATETWEHIPNTVKWPSPR 674 +++ GG + + P +DLW F T +W + + ++P PR Sbjct: 260 IFVHGGHNDTGPLSDLWLFDLETLSWTEVRSIGRFPGPR 298 Score = 29.5 bits (63), Expect = 0.48 Identities = 15/47 (31%), Positives = 22/47 (46%), Gaps = 2/47 (4%) Frame = +3 Query: 3 GEHPPSLQEHTATAHGHRIYVFGGE--AGALTNETPLWIYDTETQIW 137 G P + H AT +Y++GG G + NE LW ++ Q W Sbjct: 292 GRFPGPREGHQATTIDDTVYIYGGRDNKGLILNE--LWAFNYSQQRW 336 >SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 35.5 bits (78), Expect = 0.007 Identities = 27/84 (32%), Positives = 37/84 (44%), Gaps = 7/84 (8%) Frame = +3 Query: 111 IYDTETQIWRKLPGQV--NYTSSVRRRAR----DPRGAVSGPRGRRGHTAHALKDCLLIY 272 IYD ET+ W +PG + N T+ + D + P R GH L L+I+ Sbjct: 120 IYDIETKQWSHIPGMITENQTNDDAKEVNGSDVDEKSKHLYPSARYGHLHCVLDHYLIIF 179 Query: 273 GGYQDLKGS-TNELWAFHYESESW 341 G QDL S E+ F +S W Sbjct: 180 CG-QDLSNSYIEEINIFDLDSGKW 202 >SPAC15A10.10 |mde6||Muskelin homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 716 Score = 31.5 bits (68), Expect = 0.12 Identities = 18/73 (24%), Positives = 35/73 (47%), Gaps = 4/73 (5%) Frame = +3 Query: 282 QDLKGSTNELWAFHYESE-SWHQVRTWNPGPAR---HRHAAALHDERLYVHGGQCDLRDC 449 Q+L+ E W H ++ H++ T+ G +R H+ D +Y++GG ++ Sbjct: 230 QELQAEIIEKWPKHKLTKLRSHRIATFLKGISRRSGHQMVYNPKDNCIYLYGGWDGVKTF 289 Query: 450 ADLWFYDTMSRVW 488 +D W Y+ +W Sbjct: 290 SDFWIYNVDKDLW 302 >SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 30.7 bits (66), Expect = 0.21 Identities = 27/91 (29%), Positives = 41/91 (45%), Gaps = 11/91 (12%) Frame = +3 Query: 105 LWIYDTET-QIWR--KLPGQVNYTSSVRRRARDPRGAVSGPRGRRGH--TAHAL----KD 257 ++I+D E+ Q+ + ++ GQ N+ S+ R DP G GR GH HAL Sbjct: 285 MYIWDLESAQVQKVSRMYGQENFQPSMERFHVDPTGKYIALEGRSGHINLLHALTGQFAT 344 Query: 258 CLLIYGGYQDL--KGSTNELWAFHYESESWH 344 I G D+ +E+ Y +E WH Sbjct: 345 SFKIEGVLSDVLFTSDGSEMLVLSYGAEVWH 375 >SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces pombe|chr 1|||Manual Length = 754 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/38 (31%), Positives = 15/38 (39%) Frame = +2 Query: 134 LAQASRPSELHFLRASSREGPSWRCQWTSRSEGTHSSC 247 L RP + +AS PSW W+ G H C Sbjct: 313 LTGKKRPGDFALWKASKPGEPSWDSPWSKGRPGWHIEC 350 >SPBC15C4.05 |||ATP-dependent RNA/DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1428 Score = 27.9 bits (59), Expect = 1.5 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = -2 Query: 636 PMFLLQNETATGRCAGVNVSLPRRCRGV 553 P FLL+N T G+ + V+ PRR + Sbjct: 665 PAFLLENSTKNGKAVKIYVTEPRRISAI 692 >SPAC3F10.11c |abc2||glutathione S-conjugate-exporting ATPase Abc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1463 Score = 25.8 bits (54), Expect = 5.9 Identities = 11/36 (30%), Positives = 20/36 (55%) Frame = -3 Query: 278 PSINEETVLKGMSCVSPPTSRSTDSATRVPRATTHG 171 P ++V+ + V+ SRS+D+ + P+AT G Sbjct: 822 PLSRSQSVITSSTDVTSSASRSSDTVSNYPKATIKG 857 >SPBC1709.15c |cft2||cleavage factor two Cft2/polyadenylation factor CPSF-73 |Schizosaccharomyces pombe|chr 2|||Manual Length = 797 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/31 (38%), Positives = 16/31 (51%) Frame = +3 Query: 309 LWAFHYESESWHQVRTWNPGPARHRHAAALH 401 LW+ ESES WN +H + AAL+ Sbjct: 145 LWSLIKESESVLYAVDWNHSKDKHLNGAALY 175 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,524,419 Number of Sequences: 5004 Number of extensions: 47814 Number of successful extensions: 161 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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